Nature Cell Biology最新文献_第2页

Spatial transcriptomic characterization of a Carnegie stage 7 human embryo 卡内基7期人类胚胎的空间转录组学特征

IF 21.3 1区生物学

Nature Cell Biology Pub Date : 2025-01-10 DOI: 10.1038/s41556-024-01597-3

Lina Cui, Sirui Lin, Xiaolong Yang, Xinwei Xie, Xiaoyan Wang, Nannan He, Jingyu Yang, Xin Zhang, Xiaojian Lu, Xiaodi Yan, Yifei Guo, Bailing Zhang, Ran Li, Hefan Miao, Mei Ji, Runzhao Zhang, Leqian Yu, Zhenyu Xiao, Yulei Wei, Jingtao Guo

{"title":"Spatial transcriptomic characterization of a Carnegie stage 7 human embryo","authors":"Lina Cui, Sirui Lin, Xiaolong Yang, Xinwei Xie, Xiaoyan Wang, Nannan He, Jingyu Yang, Xin Zhang, Xiaojian Lu, Xiaodi Yan, Yifei Guo, Bailing Zhang, Ran Li, Hefan Miao, Mei Ji, Runzhao Zhang, Leqian Yu, Zhenyu Xiao, Yulei Wei, Jingtao Guo","doi":"10.1038/s41556-024-01597-3","DOIUrl":"https://doi.org/10.1038/s41556-024-01597-3","url":null,"abstract":"Gastrulation marks a pivotal stage in mammalian embryonic development, establishing the three germ layers and body axis through lineage diversification and morphogenetic movements. However, studying human gastrulating embryos is challenging due to limited access to early tissues. Here we show the use of spatial transcriptomics to analyse a fully intact Carnegie stage 7 human embryo at single-cell resolution, along with immunofluorescence validations in a second embryo. Employing 82 serial cryosections and Stereo-seq technology, we reconstructed a three-dimensional model of the embryo. Our findings reveal early specification of distinct mesoderm subtypes and the presence of the anterior visceral endoderm. Notably, primordial germ cells were located in the connecting stalk, and haematopoietic stem cell-independent haematopoiesis was observed in the yolk sac. This study advances our understanding of human gastrulation and provides a valuable dataset for future research in early human development.","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"40 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142961337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boosting cargo turnover with receptor mobility 利用受体流动性促进货物周转率

IF 21.3 1区生物学

Nature Cell Biology Pub Date : 2025-01-09 DOI: 10.1038/s41556-024-01576-8

Yi Lu, Chunmei Chang

引用次数: 0

MDM2 functions as a timer reporting the length of mitosis MDM2作为一个计时器，报告有丝分裂的长度

IF 21.3 1区生物学

Nature Cell Biology Pub Date : 2025-01-09 DOI: 10.1038/s41556-024-01592-8

Luke J. Fulcher, Tomoaki Sobajima, Caleb Batley, Ian Gibbs-Seymour, Francis A. Barr

引用次数: 0

The nuclear matrix stabilizes primed-specific genes in human pluripotent stem cells 核基质稳定了人类多能干细胞中的引物特异性基因

IF 21.3 1区生物学

Nature Cell Biology Pub Date : 2025-01-09 DOI: 10.1038/s41556-024-01595-5

Gang Ma, Xiuling Fu, Lulu Zhou, Isaac A. Babarinde, Liyang Shi, Wenting Yang, Jiao Chen, Zhen Xiao, Yu Qiao, Lisha Ma, Yuhao Ou, Yuhao Li, Chen Chang, Boping Deng, Ran Zhang, Li Sun, Guoqing Tong, Dongwei Li, Yiming Li, Andrew P. Hutchins

引用次数: 0

Transcription factor networks in cellular quiescence 细胞静止中的转录因子网络

IF 17.3 1区生物学

Nature Cell Biology Pub Date : 2025-01-09 DOI: 10.1038/s41556-024-01582-w

Mithun Mitra, Sandra L. Batista, Hilary A. Coller

{"title":"Transcription factor networks in cellular quiescence","authors":"Mithun Mitra, Sandra L. Batista, Hilary A. Coller","doi":"10.1038/s41556-024-01582-w","DOIUrl":"10.1038/s41556-024-01582-w","url":null,"abstract":"Many of the cells in mammalian tissues are in a reversible quiescent state; they are not dividing, but retain the ability to proliferate in response to extracellular signals. Quiescence relies on the activities of transcription factors (TFs) that orchestrate the repression of genes that promote proliferation and establish a quiescence-specific gene expression program. Here we discuss how the coordinated activities of TFs in different quiescent stem cells and differentiated cells maintain reversible cell cycle arrest and establish cell-protective signalling pathways. We further cover the emerging mechanisms governing the dysregulation of quiescence TF networks with age. We explore how recent developments in single-cell technologies have enhanced our understanding of quiescence heterogeneity and gene regulatory networks. We further discuss how TFs and their activities are themselves regulated at the RNA, protein and chromatin levels. Finally, we summarize the challenges associated with defining TF networks in quiescent cells. Recent developments in single-cell technologies have increased our understanding of how the coordinated activities of transcription factors in different quiescent cells and differentiated cells maintain reversible cell cycle arrest.","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"27 1","pages":"14-27"},"PeriodicalIF":17.3,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142937087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precursor occupancy controls mitochondrial import channel via proteolysis 前体占用通过蛋白水解控制线粒体输入通道

IF 21.3 1区生物学

Nature Cell Biology Pub Date : 2025-01-08 DOI: 10.1038/s41556-024-01575-9

引用次数: 0

Triacylglycerol mobilization underpins mitochondrial stress recovery 三酰基甘油动员支持线粒体应激恢复

IF 21.3 1区生物学

Nature Cell Biology Pub Date : 2025-01-08 DOI: 10.1038/s41556-024-01586-6

Zakery N. Baker, Yunyun Zhu, Rachel M. Guerra, Andrew J. Smith, Aline Arra, Lia R. Serrano, Katherine A. Overmyer, Shankar Mukherji, Elizabeth A. Craig, Joshua J. Coon, David J. Pagliarini

{"title":"Triacylglycerol mobilization underpins mitochondrial stress recovery","authors":"Zakery N. Baker, Yunyun Zhu, Rachel M. Guerra, Andrew J. Smith, Aline Arra, Lia R. Serrano, Katherine A. Overmyer, Shankar Mukherji, Elizabeth A. Craig, Joshua J. Coon, David J. Pagliarini","doi":"10.1038/s41556-024-01586-6","DOIUrl":"https://doi.org/10.1038/s41556-024-01586-6","url":null,"abstract":"Mitochondria are central to myriad biochemical processes, and thus even their moderate impairment could have drastic cellular consequences if not rectified. Here, to explore cellular strategies for surmounting mitochondrial stress, we conducted a series of chemical and genetic perturbations to Saccharomyces cerevisiae and analysed the cellular responses using deep multiomic mass spectrometry profiling. We discovered that mobilization of lipid droplet triacylglycerol stores was necessary for strains to mount a successful recovery response. In particular, acyl chains from these stores were liberated by triacylglycerol lipases and used to fuel biosynthesis of the quintessential mitochondrial membrane lipid cardiolipin to support new mitochondrial biogenesis. We demonstrate that a comparable recovery pathway exists in mammalian cells, which fail to recover from doxycycline treatment when lacking the ATGL lipase. Collectively, our work reveals a key component of mitochondrial stress recovery and offers a rich resource for further exploration of the broad cellular responses to mitochondrial dysfunction.","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"28 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Misshapen chromosomes in check by mechanics 机械控制畸形染色体

IF 17.3 1区生物学

Nature Cell Biology Pub Date : 2025-01-08 DOI: 10.1038/s41556-024-01573-x

Joana C. Macedo, Maria M. da Silva, Elsa Logarinho

引用次数: 0

Gallbladder-derived retinoic acid signalling drives reconstruction of the damaged intrahepatic biliary ducts 胆囊源性维甲酸信号驱动受损肝内胆管的重建

IF 17.3 1区生物学

Nature Cell Biology Pub Date : 2025-01-08 DOI: 10.1038/s41556-024-01568-8

Jianbo He, Shuang Li, Zhuolin Yang, Jianlong Ma, Chuanfang Qian, Zhuofu Huang, Linke Li, Yun Yang, Jingying Chen, Yunfan Sun, Tianyu Zhao, Lingfei Luo

{"title":"Gallbladder-derived retinoic acid signalling drives reconstruction of the damaged intrahepatic biliary ducts","authors":"Jianbo He, Shuang Li, Zhuolin Yang, Jianlong Ma, Chuanfang Qian, Zhuofu Huang, Linke Li, Yun Yang, Jingying Chen, Yunfan Sun, Tianyu Zhao, Lingfei Luo","doi":"10.1038/s41556-024-01568-8","DOIUrl":"10.1038/s41556-024-01568-8","url":null,"abstract":"Severe damage to the intrahepatic biliary duct (IHBD) network occurs in multiple human advanced cholangiopathies, such as primary sclerosing cholangitis, biliary atresia and end-stage primary biliary cholangitis. Whether and how a severely damaged IHBD network could reconstruct has remained unclear. Here we show that, although the gallbladder is not directly connected to the IHBD, there is a common hepatic duct (CHD) in between, and severe damage to the IHBD network induces migration of gallbladder smooth muscle cells (SMCs) to coat the CHD in mouse and zebrafish models. These gallbladder-derived, CHD-coating SMCs produce retinoic acid to activate Sox9b in the CHD, which drives proliferation and ingrowth of CHD cells into the inner liver to reconstruct the IHBD network. This study reveals a hitherto unappreciated function of the gallbladder in the recovery of injured liver, and characterizes mechanisms involved in how the gallbladder and liver communicate through inter-organ cell migration to drive tissue regeneration. Carrying out cholecystectomy will thus cause previously unexpected impairments to liver health. Luo et al. show in zebrafish and mouse that, upon intrahepatic biliary duct damage, gallbladder smooth muscle cells migrate to the common hepatic duct, where they produce retinoic acid to promote regeneration of the intrahepatic biliary duct.","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"27 1","pages":"39-47"},"PeriodicalIF":17.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

H3K36 methylation regulates cell plasticity and regeneration in the intestinal epithelium H3K36甲基化调节肠上皮细胞的可塑性和再生

IF 21.3 1区生物学

Nature Cell Biology Pub Date : 2025-01-08 DOI: 10.1038/s41556-024-01580-y

Alison R. S. Pashos, Anne R. Meyer, Cameron Bussey-Sutton, Erin S. O’Connor, Mariel Coradin, Marilyne Coulombe, Kent A. Riemondy, Sanjana Potlapelly, Brian D. Strahl, Gunnar C. Hansson, Peter J. Dempsey, Justin Brumbaugh

{"title":"H3K36 methylation regulates cell plasticity and regeneration in the intestinal epithelium","authors":"Alison R. S. Pashos, Anne R. Meyer, Cameron Bussey-Sutton, Erin S. O’Connor, Mariel Coradin, Marilyne Coulombe, Kent A. Riemondy, Sanjana Potlapelly, Brian D. Strahl, Gunnar C. Hansson, Peter J. Dempsey, Justin Brumbaugh","doi":"10.1038/s41556-024-01580-y","DOIUrl":"https://doi.org/10.1038/s41556-024-01580-y","url":null,"abstract":"Plasticity is needed during development and homeostasis to generate diverse cell types from stem and progenitor cells. Following differentiation, plasticity must be restricted in specialized cells to maintain tissue integrity and function. For this reason, specialized cell identity is stable under homeostatic conditions; however, cells in some tissues regain plasticity during injury-induced regeneration. While precise gene expression controls these processes, the regulatory mechanisms that restrict or promote cell plasticity are poorly understood. Here we use the mouse small intestine as a model system to study cell plasticity. We find that H3K36 methylation reinforces expression of cell-type-associated genes to maintain specialized cell identity in intestinal epithelial cells. Depleting H3K36 methylation disrupts lineage commitment and activates regenerative gene expression. Correspondingly, we observe rapid and reversible remodelling of H3K36 methylation following injury-induced regeneration. These data suggest a fundamental role for H3K36 methylation in reinforcing specialized lineages and regulating cell plasticity and regeneration.","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"23 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142936059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0