发布求助
文献互助 智能选刊 最新文献

Nature Cell Biology最新文献

筛选
英文 中文
Nuclear pores safeguard the integrity of the nuclear envelope 核孔保护核膜的完整性
IF 21.3 1区 生物学
Nature Cell Biology Pub Date : 2025-04-09 DOI: 10.1038/s41556-025-01648-3
Reiya Taniguchi, Clarisse Orniacki, Jan Philipp Kreysing, Vojtech Zila, Christian E. Zimmerli, Stefanie Böhm, Beata Turoňová, Hans-Georg Kräusslich, Valérie Doye, Martin Beck
{"title":"Nuclear pores safeguard the integrity of the nuclear envelope","authors":"Reiya Taniguchi, Clarisse Orniacki, Jan Philipp Kreysing, Vojtech Zila, Christian E. Zimmerli, Stefanie Böhm, Beata Turoňová, Hans-Georg Kräusslich, Valérie Doye, Martin Beck","doi":"10.1038/s41556-025-01648-3","DOIUrl":"https://doi.org/10.1038/s41556-025-01648-3","url":null,"abstract":"<p>Nuclear pore complexes (NPCs) mediate nucleocytoplasmic exchange, which is essential for eukaryotes. Mutations in the central scaffolding components of NPCs are associated with genetic diseases, but how they manifest only in specific tissues remains unclear. This is exemplified in Nup133-deficient mouse embryonic stem cells, which grow normally during pluripotency, but differentiate poorly into neurons. Here, using an innovative in situ structural biology approach, we show that <i>Nup133</i><sup>−/−</sup> mouse embryonic stem cells have heterogeneous NPCs with non-canonical symmetries and missing subunits. During neuronal differentiation, Nup133-deficient NPCs frequently disintegrate, resulting in abnormally large nuclear envelope openings. We propose that the elasticity of the NPC scaffold has a protective function for the nuclear envelope and that its perturbation becomes critical under conditions that impose an increased mechanical load onto nuclei.</p>","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"24 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143805724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Autophagy does not always decline with ageing
IF 21.3 1区 生物学
Nature Cell Biology Pub Date : 2025-04-08 DOI: 10.1038/s41556-025-01654-5
Sanjna Singh, Julian M. Carosi, Linh Dang, Timothy J. Sargeant
{"title":"Autophagy does not always decline with ageing","authors":"Sanjna Singh, Julian M. Carosi, Linh Dang, Timothy J. Sargeant","doi":"10.1038/s41556-025-01654-5","DOIUrl":"https://doi.org/10.1038/s41556-025-01654-5","url":null,"abstract":"Autophagy has long been presumed to decline with age, underpinning its designation as a hallmark of ageing. However, emerging evidence challenges this notion, showing tissue-specific variability and, in some cases, age-related increases in autophagic activity. Understanding these dynamics is vital for targeted therapeutic strategies.","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"8 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143797981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Emerging roles of lysine lactyltransferases and lactylation
IF 21.3 1区 生物学
Nature Cell Biology Pub Date : 2025-04-04 DOI: 10.1038/s41556-025-01635-8
Zhi Zong, Jiang Ren, Bing Yang, Long Zhang, Fangfang Zhou
{"title":"Emerging roles of lysine lactyltransferases and lactylation","authors":"Zhi Zong, Jiang Ren, Bing Yang, Long Zhang, Fangfang Zhou","doi":"10.1038/s41556-025-01635-8","DOIUrl":"https://doi.org/10.1038/s41556-025-01635-8","url":null,"abstract":"<p>Given its various roles in cellular functions, lactate is no longer considered a waste product of metabolism and lactate sensing is a pivotal step in the transduction of lactate signals. Lysine lactylation is a recently identified post-translational modification that serves as an intracellular mechanism of lactate sensing and transfer. Although acetyltransferases such as p300 exhibit general acyl transfer activity, no bona fide lactyltransferases have been identified. Recently, the protein synthesis machinery, alanyl-tRNA synthetase 1 (AARS1), AARS2 and their <i>Escherichia coli</i> orthologue AlaRS, have been shown to be able to sense lactate and mediate lactyl transfer and are thus considered pan-lactyltransferases. Here we highlight the mechanisms and functions of these lactyltransferases and discuss potential strategies that could be exploited for the treatment of human diseases.</p>","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"21 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The guinea pig serves as an alternative model to study human preimplantation development
IF 21.3 1区 生物学
Nature Cell Biology Pub Date : 2025-04-04 DOI: 10.1038/s41556-025-01642-9
Jesica Romina Canizo, Cheng Zhao, Sophie Petropoulos
{"title":"The guinea pig serves as an alternative model to study human preimplantation development","authors":"Jesica Romina Canizo, Cheng Zhao, Sophie Petropoulos","doi":"10.1038/s41556-025-01642-9","DOIUrl":"https://doi.org/10.1038/s41556-025-01642-9","url":null,"abstract":"<p>Preimplantation development is an important window of human embryogenesis. However, ethical constraints and the limitations involved in studying human embryos often necessitate the use of alternative model systems. Here we identify the guinea pig as a promising small animal model to study human preimplantation development. Using single-cell RNA-sequencing, we generated an atlas of guinea pig preimplantation development, revealing its close resemblance to early human embryogenesis in terms of the timing of compaction, early-, mid- and late-blastocyst formation, and implantation, and the spatio-temporal expression of key lineage markers. We also show conserved roles of Hippo, MEK-ERK and JAK-STAT signalling. Furthermore, multi-species analysis highlights the spatio-temporal expression of conserved and divergent genes during preimplantation development and pluripotency. The guinea pig serves as a valuable animal model for advancing preimplantation development and stem cell research, and can be leveraged to better understand the longer-term impact of early exposures on offspring outcomes.</p>","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"58 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Supra-second tracking and live-cell karyotyping reveal principles of mitotic chromosome dynamics
IF 21.3 1区 生物学
Nature Cell Biology Pub Date : 2025-04-04 DOI: 10.1038/s41556-025-01637-6
Rumen Stamatov, Sonya Uzunova, Yoana Kicheva, Maria Karaboeva, Tavian Blagoev, Stoyno Stoynov
{"title":"Supra-second tracking and live-cell karyotyping reveal principles of mitotic chromosome dynamics","authors":"Rumen Stamatov, Sonya Uzunova, Yoana Kicheva, Maria Karaboeva, Tavian Blagoev, Stoyno Stoynov","doi":"10.1038/s41556-025-01637-6","DOIUrl":"https://doi.org/10.1038/s41556-025-01637-6","url":null,"abstract":"<p>Mitotic chromosome dynamics are essential for the three-dimensional organization of the genome during the cell cycle, but the spatiotemporal characteristics of this process remain unclear due to methodological challenges. While Hi-C methods capture interchromosomal contacts, they lack single-cell temporal dynamics, whereas microscopy struggles with bleaching and phototoxicity. Here, to overcome these limitations, we introduce Facilitated Segmentation and Tracking of Chromosomes in Mitosis Pipeline (FAST CHIMP), pairing time-lapse super-resolution microscopy with deep learning. FAST CHIMP tracked all human chromosomes with 8-s resolution from prophase to telophase, identified 15 out of 23 homologue pairs in single cells and compared chromosomal positioning between mother and daughter cells. It revealed a centrosome-motion-dependent flow that governs the mapping between chromosome locations at prophase and their metaphase plate position. In addition, FAST CHIMP measured supra-second dynamics of intra- and interchromosomal contacts. This tool adds a dynamic dimension to the study of chromatin behaviour in live cells, promising advances beyond the scope of existing methods.</p>","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"33 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modulation of bone marrow haematopoietic stem cell activity as a therapeutic strategy after myocardial infarction: a preclinical study
IF 21.3 1区 生物学
Nature Cell Biology Pub Date : 2025-04-02 DOI: 10.1038/s41556-025-01639-4
Jasmin Rettkowski, Mari Carmen Romero-Mulero, Indranil Singh, Carolin Wadle, Jan Wrobel, Diana Chiang, Natalie Hoppe, Julian Mess, Katharina Schönberger, Maria-Eleni Lalioti, Karin Jäcklein, Beatriz SilvaRego, Timon Bühler, Noémie Karabacz, Mirijam Egg, Helen Demollin, Nadine Obier, Yu Wei Zhang, Claus Jülicher, Anne Hetkamp, Martin Czerny, Michael-Jason Jones, Hana Seung, Ritika Jain, Constantin von zur Mühlen, Alexander Maier, Achim Lother, Ingo Hilgendorf, Peter van Galen, Antonia Kreso, Dirk Westermann, Alejo E. Rodriguez-Fraticelli, Timo Heidt, Nina Cabezas-Wallscheid
{"title":"Modulation of bone marrow haematopoietic stem cell activity as a therapeutic strategy after myocardial infarction: a preclinical study","authors":"Jasmin Rettkowski, Mari Carmen Romero-Mulero, Indranil Singh, Carolin Wadle, Jan Wrobel, Diana Chiang, Natalie Hoppe, Julian Mess, Katharina Schönberger, Maria-Eleni Lalioti, Karin Jäcklein, Beatriz SilvaRego, Timon Bühler, Noémie Karabacz, Mirijam Egg, Helen Demollin, Nadine Obier, Yu Wei Zhang, Claus Jülicher, Anne Hetkamp, Martin Czerny, Michael-Jason Jones, Hana Seung, Ritika Jain, Constantin von zur Mühlen, Alexander Maier, Achim Lother, Ingo Hilgendorf, Peter van Galen, Antonia Kreso, Dirk Westermann, Alejo E. Rodriguez-Fraticelli, Timo Heidt, Nina Cabezas-Wallscheid","doi":"10.1038/s41556-025-01639-4","DOIUrl":"https://doi.org/10.1038/s41556-025-01639-4","url":null,"abstract":"<p>Myocardial infarction (MI) is a major global health concern. Although myeloid cells are crucial for tissue repair in emergency haematopoiesis after MI, excessive myelopoiesis can exacerbate scarring and impair cardiac function. Bone marrow (BM) haematopoietic stem cells (HSCs) have the unique capability to replenish the haematopoietic system, but their role in emergency haematopoiesis after MI has not yet been established. Here we collected human sternal BM samples from over 150 cardiac surgery patients, selecting 49 with preserved cardiac function. We show that MI causes detrimental transcriptional and functional changes in human BM HSCs. Lineage tracing experiments suggest that HSCs are contributors of pro-inflammatory myeloid cells infiltrating cardiac tissue after MI. Therapeutically, enforcing HSC quiescence with the vitamin A metabolite 4-oxo-retinoic acid dampens inflammatory myelopoiesis, thereby modulating tissue remodelling and preserving long-term cardiac function after MI.</p>","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"105 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Publisher Correction: m6A RNA methylation orchestrates transcriptional dormancy during paused pluripotency
IF 21.3 1区 生物学
Nature Cell Biology Pub Date : 2025-04-02 DOI: 10.1038/s41556-025-01664-3
Evelyne Collignon, Brandon Cho, Giacomo Furlan, Julie Fothergill-Robinson, Sylvia-Bryn Martin, Sarah A. McClymont, Robert L. Ross, Patrick A. Limbach, Miguel Ramalho-Santos
{"title":"Publisher Correction: m6A RNA methylation orchestrates transcriptional dormancy during paused pluripotency","authors":"Evelyne Collignon, Brandon Cho, Giacomo Furlan, Julie Fothergill-Robinson, Sylvia-Bryn Martin, Sarah A. McClymont, Robert L. Ross, Patrick A. Limbach, Miguel Ramalho-Santos","doi":"10.1038/s41556-025-01664-3","DOIUrl":"https://doi.org/10.1038/s41556-025-01664-3","url":null,"abstract":"<p>Correction to: <i>Nature Cell Biology</i> https://doi.org/10.1038/s41556-023-01212-x, published online 7 September 2023.</p>","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"32 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phosphoinositide signalling in cell motility and adhesion
IF 21.3 1区 生物学
Nature Cell Biology Pub Date : 2025-04-01 DOI: 10.1038/s41556-025-01647-4
Xiaoting Hou, Chang Ren, Jing Jin, Yu Chen, Xinyu Lyu, Kangle Bi, Noah D. Carrillo, Vincent L. Cryns, Richard A. Anderson, Jichao Sun, Mo Chen
{"title":"Phosphoinositide signalling in cell motility and adhesion","authors":"Xiaoting Hou, Chang Ren, Jing Jin, Yu Chen, Xinyu Lyu, Kangle Bi, Noah D. Carrillo, Vincent L. Cryns, Richard A. Anderson, Jichao Sun, Mo Chen","doi":"10.1038/s41556-025-01647-4","DOIUrl":"https://doi.org/10.1038/s41556-025-01647-4","url":null,"abstract":"<p>Cell motility and adhesion are fundamental components for diverse physiological functions, including embryonic development, immune responses and tissue repair. Dysregulation of these processes can lead to a range of diseases, including cancer. Cell motility and adhesion are complex and often require regulation by an intricate network of signalling pathways, with phosphatidylinositol phosphates (PIPs) having a central role. PIPs are derived from phosphatidylinositol phosphorylation and are instrumental in mediating membrane dynamics, intracellular trafficking, cytoskeletal organization and signal transduction, all of which are crucial for cellular responses to environmental stimuli. Here we discuss the mechanisms through which PIPs modulate cell motility and adhesion by examining their roles at focal adhesions, within the cytoskeleton, at protein scaffolds and in the nucleus. By providing a comprehensive overview of PIP signalling, this Review underscores their significance in maintaining cellular homeostasis and highlights their potential as therapeutic targets in diseases characterized by aberrant cell motility and adhesion.</p>","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"43 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lysosome repair fails in ageing and Alzheimer’s disease
IF 21.3 1区 生物学
Nature Cell Biology Pub Date : 2025-03-26 DOI: 10.1038/s41556-024-01608-3
Céline Vrancx, Wim Annaert
{"title":"Lysosome repair fails in ageing and Alzheimer’s disease","authors":"Céline Vrancx, Wim Annaert","doi":"10.1038/s41556-024-01608-3","DOIUrl":"https://doi.org/10.1038/s41556-024-01608-3","url":null,"abstract":"Decades of research have linked lysosomal failure to Alzheimer’s disease, but the role of ageing has remained unknown, hindered by the absence of in vitro models that combine both ageing and Alzheimer’s. A study now identifies how disrupted lysosome repair fuels dysproteostasis and inflammation in both aged and diseased neurons.","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"57 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteostasis and lysosomal repair deficits in transdifferentiated neurons of Alzheimer’s disease
IF 21.3 1区 生物学
Nature Cell Biology Pub Date : 2025-03-26 DOI: 10.1038/s41556-025-01623-y
Ching-Chieh Chou, Ryan Vest, Miguel A. Prado, Joshua Wilson-Grady, Joao A. Paulo, Yohei Shibuya, Patricia Moran-Losada, Ting-Ting Lee, Jian Luo, Steven P. Gygi, Jeffery W. Kelly, Daniel Finley, Marius Wernig, Tony Wyss-Coray, Judith Frydman
{"title":"Proteostasis and lysosomal repair deficits in transdifferentiated neurons of Alzheimer’s disease","authors":"Ching-Chieh Chou, Ryan Vest, Miguel A. Prado, Joshua Wilson-Grady, Joao A. Paulo, Yohei Shibuya, Patricia Moran-Losada, Ting-Ting Lee, Jian Luo, Steven P. Gygi, Jeffery W. Kelly, Daniel Finley, Marius Wernig, Tony Wyss-Coray, Judith Frydman","doi":"10.1038/s41556-025-01623-y","DOIUrl":"https://doi.org/10.1038/s41556-025-01623-y","url":null,"abstract":"<p>Ageing is the most prominent risk factor for Alzheimer’s disease (AD). However, the cellular mechanisms linking neuronal proteostasis decline to the characteristic aberrant protein deposits in the brains of patients with AD remain elusive. Here we develop transdifferentiated neurons (tNeurons) from human dermal fibroblasts as a neuronal model that retains ageing hallmarks and exhibits AD-linked vulnerabilities. Remarkably, AD tNeurons accumulate proteotoxic deposits, including phospho-tau and amyloid β, resembling those in APP mouse brains and the brains of patients with AD. Quantitative tNeuron proteomics identify ageing- and AD-linked deficits in proteostasis and organelle homeostasis, most notably in endosome–lysosomal components. Lysosomal deficits in aged tNeurons, including constitutive lysosomal damage and ESCRT-mediated lysosomal repair defects, are exacerbated in AD tNeurons and linked to inflammatory cytokine secretion and cell death. Providing support for the centrality of lysosomal deficits in AD, compounds ameliorating lysosomal function reduce amyloid β deposits and cytokine secretion. Thus, the tNeuron model system reveals impaired lysosomal homeostasis as an early event of ageing and AD.</p>","PeriodicalId":18977,"journal":{"name":"Nature Cell Biology","volume":"29 1","pages":""},"PeriodicalIF":21.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信