南方医科大学学报杂志最新文献

{"title":"[Latest research progress of rare earth-magnesium alloys in orthopedics].","authors":"Zhengming Sun, Kun Zuo, Xinke Zhu, Hao Yue, Zhengchao Gao","doi":"10.12122/j.issn.1673-4254.2025.02.24","DOIUrl":"10.12122/j.issn.1673-4254.2025.02.24","url":null,"abstract":"<p><p>Due to their good properties of elastic modulus, degradability and ability to promote bone repair, magnesium alloys have become a research hotspot in research of orthopedic implants. Nevertheless, most of the biomedical magnesium alloys currently available fail to meet the requirements in orthopedics because of their rapid degradation after implantation. Rare earth-magnesium alloys possess excellent corrosion resistance and are expected to become important materials as clinical orthopedic implants. This review summarizes the recent progress in studies of the physiological functions of rare earth elements, the effects of supplementation of rare earth elements on biomechanical properties and the <i>in vitro</i> and <i>in vivo</i> biocompatibility of magnesium alloys, and their contribution to tendon-bone healing, addressing also the current clinical orthopedic applications of different rare earth-magnesium alloys, challenges, and future strategies for improving these alloys.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 2","pages":"437-442"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A rapid method for detecting <i>prfA</i> and <i>hly</i> toxin genes of <i>Listeria monocytogenes</i> using double nucleic acid colloidal gold strips].","authors":"Yan Liu, Jianyu Yang, Yujiao Zhou, Wenbo Ding, Xianyu Zhang, Linran Gao, Beizhen Pan, Jifei Yang, Yundong Zhao","doi":"10.12122/j.issn.1673-4254.2025.02.20","DOIUrl":"10.12122/j.issn.1673-4254.2025.02.20","url":null,"abstract":"<p><strong>Objectives: </strong>To detect <i>prfA</i> and <i>hly</i> toxin genes of <i>Listeria monocytogenes</i> using polymerase chain reaction (PCR) and colloidal gold technology.</p><p><strong>Methods: </strong><i>L. monocytogenes</i> DNA was extracted by boiling method. With <i>prfA</i> and <i>hly</i> of <i>L. monocytogenes</i> as the target genes, the 5' ends of upstream and downstream primers of <i>prfA</i> gene were labeled with 6-FAM and biotin, and the 5' ends of upstream and downstream primers of <i>hly</i> gene were labeled with digoxin and biotin, respectively, to establish the toxin gene detection method. Using cloning transformation, sequencing analysis, cloning of positive control products, the detection kid was developed and its specificity, sensitivity, reproducibility and stability were tested, followed by verification with sample testing.</p><p><strong>Results: </strong>The concentration of <i>L. monocytogenes</i> DNA extracted by boiling method was 148.81±0.97 ng/μL, and the A₂₆₀/A₂₈₀ ratio ranged from 1.8 to 2.0. The PCR products showed a 100% homology with the gene sequences in GenBank database after cloning, transformation and sequencing. The colloidal gold strip yielded positive results only for <i>L. monocytogenes</i> samples without cross-reactions with <i>Staphylococcus aureus</i>, <i>Escherichia coli</i> or <i>Bacillus cereus</i>, and its minimum detection limit was 10^-2 ng/μL, demonstrating a 10-fold greater sensitivity of the test than agarose gel electrophoresis. The test also showed good reproducibility of the results when performed by different operators with good stability of the test strips after storage for 6 to 12 months. The test results showed that this kit could accurately and quickly detect <i>L.monocytogenes</i> in the test samples.</p><p><strong>Conclusions: </strong>The detection kit developed in this study can simultaneously detect <i>prfA</i> and <i>hly</i> toxin genes of <i>L. monocytogenes</i> with good specificity, sensitivity, reproducibility and stability for use in food safety inspection.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 2","pages":"387-394"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Low-intensity pulsed ultrasound combined with nystatin treatment synergistically inhibits vaginal <i>Candida albicans</i> biofilm infection in rabbits].","authors":"Mengyao Xie, Min Yang, Xin Li, Yonghong DU","doi":"10.12122/j.issn.1673-4254.2025.02.10","DOIUrl":"10.12122/j.issn.1673-4254.2025.02.10","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the efficacy of low-intensity pulsed ultrasound (LIPUS) combined with nystatin for treatment of vaginal <i>Candida albicans</i> biofilm infection.</p><p><strong>Methods: </strong><i>In vitro</i> cultured <i>Candida albicans</i> biofilm were treated with LIPUS, nystatin, or both, and the minimum inhibitory concentration (MIC) of nystatin was determined. Crystal violet staining, confocal laser microscopy (CLSM) and scanning electron microscopy were used to quantify the biofilm and observe the activity and morphological changes of the biofilms; DCFH-DA was used to detect the changes in reactive oxygen species (ROS). Twenty female New Zealand White rabbits with vaginal inoculation of <i>Candida albicans</i> biofilm were randomized into 4 groups for treatment with normal saline, LIPUS, nystatin, or both LIPUS and nystatin. The changes in vulvar symptoms of the rabbits were observed, and the histopathological and ultrastructural changes of the vagina before and after treatment were observed using HE staining and transmission electron microscopy.</p><p><strong>Results: </strong>In the combined treatment group, the MIC₅₀ and MIC₈₀ of nystatin in <i>Candida albicans</i> biofilms were both reduced by 50% compared with those in nystatin group, and the biofilm clearance rate increased by 26% and 68% compared with nystatin and LIPUS groups, respectively. Compared with nystatin and LIPUS treatment alone, the combined treatment produced stronger effects for inhibiting biofilm activity, causing structural disruption and promoting ROS production. In the rabbit models, the combined treatment more effectively improved vulvar symptoms and inflammatory infiltration, reduced residual vaginal hyphae/strains, and improved ultrastructure of the vaginal epithelium than LIPUS and nystatin treatment alone.</p><p><strong>Conclusions: </strong>LIPUS combined with nystatin produces a significant synergistic antifungal effect against <i>Candida albicans</i> biofilm both <i>in vitro</i> and <i>in vivo</i>.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 2","pages":"296-303"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[An efficient and lightweight skin pathology detection method based on multi-scale feature fusion using an improved RT-DETR model].","authors":"Yuying Ren, Lingxiao Huang, Fang DU, Xinbo Yao","doi":"10.12122/j.issn.1673-4254.2025.02.22","DOIUrl":"10.12122/j.issn.1673-4254.2025.02.22","url":null,"abstract":"<p><strong>Objectives: </strong>The presence of multi-scale skin lesion regions and image noise interference and limited resources of auxiliary diagnostic equipment affect the accuracy of skin disease detection in skin disease detection tasks. To solve these problems, we propose a highly efficient and lightweight skin disease detection model using an improved RT-DETR model.</p><p><strong>Methods: </strong>A lightweight FasterNet was introduced as the backbone network and the FasterNetBlock module was parametrically refined. A Convolutional and Attention Fusion Module (CAFM) was used to replace the multi-head self-attention mechanism in the neck network to enhance the ability of the AIFI-CAFM module for capturing global dependencies and local detail information. The DRB-HSFPN feature pyramid network was designed to replace the Cross-Scale Feature Fusion Module (CCFM) to allow the integration of contextual information across different scales to improve the semantic feature expression capacity of the neck network. Finally, combining the advantages of Inner-IoU and EIoU, the Inner-EIoU was used to replace the original loss function GIOU to further enhance the model's inference accuracy and convergence speed.</p><p><strong>Results: </strong>The experimental results on the HAM10000 dataset showed that the improved RT-DETR model, as compared with the original model, had increased mAP@50 and mAP@50:95 by 4.5% and 2.8%, respectively, with a detection speed of 59.1 frames per second (FPS). The improved model had a parameter count of 10.9 M and a computational load of 19.3 GFLOPs, which were reduced by 46.0% and 67.2% compared to those of the original model, validating the effectiveness of the improved model.</p><p><strong>Conclusions: </strong>The proposed SD-DETR model significantly improves the performance of skin disease detection tasks by effectively extracting and integrating multi-scale features while reducing both parameter count and computational load.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 2","pages":"409-421"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Quercetin mitigates HIV-1 gp120-induced rat astrocyte neurotoxicity <i>via</i> promoting G3BP1 disassembly in stress granules].","authors":"Pengwei Huang, Jie Chen, Jinhu Zou, Xuefeng Gao, Hong Cao","doi":"10.12122/j.issn.1673-4254.2025.02.11","DOIUrl":"10.12122/j.issn.1673-4254.2025.02.11","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the effect of quercetin for mitigating HIV-1 gp120-induced astrocyte neurotoxicity and its underlying mechanism.</p><p><strong>Methods: </strong>Primary rat astrocytes were isolated and treated with quercetin, HIV-1 gp120, or gradient concentrations of quercetin combined with HIV-1 gp120. The formation of stress granules (SGs) in the treated cells was observed with immunofluorescence assay, and the levels of oxidative stress markers and protein expressions were measured using specific assay kits and Western blotting. HIV-1 gp120 transgenic mice were treated with quercetin (50 mg/kg) by gavage for 4 weeks, and the changes in cognitive functions and oxidative stress levels were examined by behavioral assessments, oxidative stress index analysis in serum, and immunohistochemical and Western blotting of the brain tissue.</p><p><strong>Results: </strong>In primary rat astrocytes, treatment with quercetin significantly reduced HIV-1 gp120-induced SG formation, increased the levels of antioxidant indexes, decreased the levels of oxidative substances, and up-regulated protein level associated with SG depolymerization. In the transgenic mouse models, quercetin obviously improved the cognitive function of the rats, reduced oxidative stress levels, and promoted the expression of proteins associate with SG depolymerization in the brain tissues.</p><p><strong>Conclusions: </strong>Quercetin mitigates HIV-1 gp120-induced astrocyte neurotoxicity and cognitive function impairment by inhibiting oxidative stress, enhancing expressions of SG depolymerization-related proteins, and promoting SG disassembly, suggesting the value of quercetin as a potential therapeutic agent for neuroprotection in HIV-associated neurocognitive disorders.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 2","pages":"304-312"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Ag₂Se nanoparticles suppress growth of murine esophageal cancer allograft in mice by eliminating <i>Porphyromonas gingivalis</i>].","authors":"Yali Zhao, Jiayi Li, Bianli Gu, Pan Chen, Li Zhang, Xiaoman Zhang, Pingjuan Yang, Linlin Shi, Shegan Gao","doi":"10.12122/j.issn.1673-4254.2025.02.05","DOIUrl":"10.12122/j.issn.1673-4254.2025.02.05","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the efficacy of Ag₂Se nanoparticles for eliminating intracellular <i>Porphyromonas gingivalis</i> (<i>P. gingivalis</i>) in esophageal cancer and examine the effect of <i>P. gingivalis</i> clearance on progression of esophageal cancer.</p><p><strong>Methods: </strong>Ag₂Se nanoparticles were synthesized <i>via</i> a chemical synthesis method. The effects of Ag₂Se nanoparticles on <i>P. gingivalis</i> viability and colony-forming ability were assessed using fluorescence staining and colony formation assays. In a mouse model bearing subcutaneous murine esophageal cancer cell allograft with <i>P. gingivalis</i> infection, the effect of treatment with Ag₂Se nanoparticles on the abundance of <i>P. gingivalis</i> in the tumor tissues was quantified using RNAscope <i>in situ</i> hybridization and quantitative polymerase chain reaction (qPCR), and the changes in tumor volume were monitored. The biosafety of Ag₂Se nanoparticles was assessed by examining liver and kidney functions and pathological changes in the major organs of the mice.</p><p><strong>Results: </strong>Transmission electron microscopy revealed that the synthesized Ag₂Se nanoparticles were uniformly dispersed spherical particles with a diameter around 50 nm. <i>In vitro</i> experiments demonstrated that exposure to Ag₂Se nanoparticles significantly reduced the viability and clonal proliferation capacity of <i>P. gingivalis</i> in a dose-dependent manner. In the tumor-bearing mice, treatment with Ag₂Se nanoparticles significantly reduced the abundance of <i>P. gingivalis</i> in tumor tissues and suppressed tumor cell proliferation. No significant damages to the liver and kidney functions or the major organs were observed in Ag₂Se nanoparticle-treated mice, demonstrating good biocompatibility of Ag₂Se nanoparticles.</p><p><strong>Conclusions: </strong>Ag₂Se nanoparticles exhibit significant bactericidal and inhibitory effects against <i>P. gingivalis</i>, and can effectively eliminate intracellular <i>P. gingivalis</i> to suppress the growth of esophageal cancer allograft in mice, suggesting the potential of Ag₂Se nanoparticles in the treatment of esophageal cancer.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 2","pages":"245-253"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A segmented backprojection tensor degradation feature encoding model for motion artifacts correction in dental cone beam computed tomography].","authors":"Zhixiong Zeng, Yongbo Wang, Zongyue Lin, Zhaoying Bian, Jianhua Ma","doi":"10.12122/j.issn.1673-4254.2025.02.23","DOIUrl":"10.12122/j.issn.1673-4254.2025.02.23","url":null,"abstract":"<p><strong>Objectives: </strong>We propose a segmented backprojection tensor degradation feature encoding (SBP-MAC) model for motion artifact correction in dental cone beam computed tomography (CBCT) to improve the quality of the reconstructed images.</p><p><strong>Methods: </strong>The proposed motion artifact correction model consists of a generator and a degradation encoder. The segmented limited-angle reconstructed sub-images are stacked into the tensors and used as the model input. A degradation encoder is used to extract spatially varying motion information in the tensor, and the generator's skip connection features are adaptively modulated to guide the model for correcting artifacts caused by different motion waveforms. The artifact consistency loss function was designed to simplify the learning task of the generator.</p><p><strong>Results: </strong>The proposed model could effectively remove motion artifacts and improve the quality of the reconstructed images. For simulated data, the proposed model increased the peak signal-to-noise ratio by 8.28%, increased the structural similarity index measurement by 2.29%, and decreased the root mean square error by 23.84%. For real clinical data, the proposed model achieved the highest expert score of 4.4221 (against a 5-point scale), which was significantly higher than those of all the other comparison methods.</p><p><strong>Conclusions: </strong>The SBP-MAC model can effectively extract spatially varying motion information in the tensors and achieve adaptive artifact correction from the tensor domain to the image domain to improve the quality of reconstructed dental CBCT images.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 2","pages":"422-436"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[High expression of apolipoprotein C1 promotes proliferation and inhibits apoptosis of papillary thyroid carcinoma cells by activating the JAK2/STAT3 signaling pathway].","authors":"Yu Bin, Ziwen Li, Suwei Zuo, Sinuo Sun, Min Li, Jiayin Song, Xu Lin, Gang Xue, Jingfang Wu","doi":"10.12122/j.issn.1673-4254.2025.02.17","DOIUrl":"10.12122/j.issn.1673-4254.2025.02.17","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the expression of apolipoprotein C1 (APOC1) in papillary thyroid carcinoma (PTC) and its effects on proliferation and apoptosis of PTC cells.</p><p><strong>Methods: </strong>The expression level of APOC1 in PTC and its impact on prognosis were analyzed using GEPIA 2 and Kaplan-Meier databases. Immunohistochemistry (IHC) and Western blotting were used to detect the expression of APOC1 in PTC and adjacent tissues and in 3 PTC cell lines and normal thyroid Nthyori 3-1 cells. In TPC-1 and BCPAP cells, the effect of Lipofectamine 2000-mediated transfection with APOC1 siRNA or an APOC1-overexpressing plasmid on cell growth and colony formation ability were examined by observing the growth curves and using colony-forming assay. The changes in cell cycle and apoptosis of the transfected cells were analyzed with flow cytometry. RT-qPCR and Western blotting were used to detect the changes in expressions of P21, P27, CDK4, cyclin D1, Bcl-2, Bax, caspase-3 and caspase-9 and the key proteins in the JAK2/STAT3 signaling pathway.</p><p><strong>Results: </strong>APOC1 expression was significantly higher in PTC tissues and the 3 PTC cell lines than in the adjacent tissues and Nthyori 3-1 cells, respectively. In TPC-1 and BCPAP cells, APOC1 knockdown obviously reduced cell proliferative activity, increased the percentage of G0/G1 phase cells, lowered the percentages of S and G2 phase cells, promoted cell apoptosis, and downregulated mRNA and protein expression levels of CDK4, cyclin D1 and Bcl-2 and the protein levels of p-JAK2 and p-STAT3. APOC1 overexpression in the cells produced the opposite effects on cell proliferation, apoptosis, cell cycle and the mRNA and protein expressions. The application of AG490, a JAK2 inhibitor, strongly attenuated APOC1 overexpression-induced activation of the JAK2/STAT3 signaling pathway in BCPAP cells.</p><p><strong>Conclusions: </strong>APOC1 overexpression promotes proliferation and inhibits apoptosis of PTC cells possibly by activating the JAK2/STAT3 signaling pathway and accelerating cell cycle progression.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 2","pages":"359-370"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[High expression of miR-124 improves cognitive function of sleep-deprived rats by modulating the PI3K/AKT signaling pathway].","authors":"Yuejiao Pei, Huimin Liu, Yu Xin, Bo Liu","doi":"10.12122/j.issn.1673-4254.2025.02.15","DOIUrl":"10.12122/j.issn.1673-4254.2025.02.15","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the molecular mechanism by which miR-124 affects cognitive function of sleep-deprived rats.</p><p><strong>Methods: </strong>Fifty-four adult male SD rats were randomized into 6 groups (<i>n</i>=9), including a normal control group, a sleep deprivation (SD) model group, and 4 intracerebral microinjection groups in which the rats were subjected to stereotactic injection of miR-124 agomir, miR-124 agomir NC, miR-124 antagomir, or miR-124 antagomir into the lateral ventricle 7 days before SD modeling. The cognitive functions of the rats were evaluated with Morris water maze test, and pathological changes in the hippocampus were observed using HE staining. The expression level of miR-124 in hippocampal tissues of the rats was detected with qRT-PCR, and the expression level of apoptosis-related proteins and signaling pathway proteins were determined using Western blotting.</p><p><strong>Results: </strong>In Morris water maze test, the SD rat models treated with miR-124 agomir showed a significantly shorter escape latency and fewer platform crossings with increased percentage of time and swimming distance in the fourth quadrant as compared with those in SD model group, while the rats treated with miR-124 antagomir exhibited worsened performance in the test. In the SD rat models, treatment with miR-124 agomir obviously lessened pathological changes in the hippocampus, while treatment with miR-124 antagomir significantly worsened the pathological changes. Compared with those in SD model group, the miR-124 agomir-treated rats showed an increased hippocampal expression of miR-124 with upregulated protein expressions of PI3K, p-AKT/AKT, and Bcl-2 and downregulated expressions of Bax and caspase-3 proteins, while rats treated with miR-124 antagomir showed significantly decreased hippocampal expression of miR-124 with lowered expressions of PI3K, p-AKT/AKT and Bcl-2 proteins and increased Bax and caspase-3 protein expressions.</p><p><strong>Conclusions: </strong>High expression of miR-124 alleviates SD-induced cognitive decline and neuronal apoptosis in rats by activating the PI3K/AKT signaling pathway.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 2","pages":"340-346"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[<i>Jiawei Xiaoyao</i> Pills improves depression-like behavior in rats by regulating neurotransmitters, inhibiting inflammation and oxidation and modulating intestinal flora].","authors":"Ying Liu, Borui Li, Yongcai Li, Lubo Chang, Jiao Wang, Lin Yang, Yonggang Yan, Kai Qv, Jiping Liu, Gang Zhang, Xia Shen","doi":"10.12122/j.issn.1673-4254.2025.02.16","DOIUrl":"10.12122/j.issn.1673-4254.2025.02.16","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the bioactive components in <i>Jiawei Xiaoyao</i> Pills (JWXYP) and their mechanisms for alleviating depression-like behaviors.</p><p><strong>Methods: </strong>The active compounds, key targets, and pathways of JWXYP were identified using TCMSP and TCMIP databases. Thirty-six SD rats were randomized equally into 6 groups including a control group and 5 chronic unpredictable mild stress (CUMS)-induced depression groups. After modeling, the 5 model groups were treated with daily gavage of normal saline, 1.8 mg/kg fluoxetine hydrochloride (positive control drug), or JWXYP at 1.44, 2.88, and 4.32 g/kg. The depression-like behaviors of the rats were evaluated using behavioral tests, and pathological changes in the liver and hippocampus were examined with HE staining. The biochemical indicators in the serum and brain tissues were detected using ELISA. Serum metabolomics analysis was performed to identify the differential metabolites using OPLS-DA, and gut microbiota changes were analyzed using 16S rDNA sequencing.</p><p><strong>Results: </strong>Network pharmacology revealed that menthone and paeonol in JWXYP were capable of penetrating the blood-brain barrier to regulate inflammatory pathways and protect the nervous system. In the rat models subjected to CUMS, treatment with JWXYP significantly improved body weight loss, sucrose preference and open field activities, reduced liver inflammation, alleviated structural changes in the hippocampal neurons, decreased serum levels of TNF‑α, IL-1β, IL-6 and LBP, and increased 5-HT and VIP concentrations in the serum and brain tissue, and these effects were the most pronounced in the high-dose group. Metabolomics analysis showed changes in such metabolites as indole-3-acetamide and acetyl-L-carnitine in JWXYP-treated rats, involving the pathways for bile acid biosynthesis and amino acid metabolism. 16S rDNA analysis demonstrated increased gut microbiota diversity and increased abundance of Lactobacillus species in JWXYP-treated rats.</p><p><strong>Conclusions: </strong>JWXYP alleviates depression-like symptoms in rats by regulating the neurotransmitters, inhibiting inflammation and oxidation, and modulating gut microbiota.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 2","pages":"347-358"},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}