南方医科大学学报杂志最新文献

{"title":"[Cannabidiol regulates circadian rhythm to improve sleep disorders following general anesthesia in rats].","authors":"Xinshun Wu, Jingcao Li, Ying Liu, Renhong Qiu, Henglin Wang, Rui Xye, Yang Zhang, Shuo Li, Qiongyin Fan, Huajin Dong, Youzhi Zhang, Jiangbei Cao","doi":"10.12122/j.issn.1673-4254.2025.04.09","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.09","url":null,"abstract":"<p><strong>Objectives: </strong>To assess the regulatory effect of cannabidiol (CBD) on circadian rhythm sleep disorders following general anesthesia and explore its potential mechanism in a rat model of propofol-induced rhythm sleep disorder.</p><p><strong>Methods: </strong>An electrode was embedded in the skull for cortical EEG recording in 24 male SD rats, which were randomized into control, propofol, CBD treatment, and diazepam treatment groups (<i>n</i>=6). Eight days later, a single dose of propofol (10 mg/kg) was injected via the tail vein with anesthesia maintenance for 3 h in the latter 3 groups, and daily treatment with saline, CBD or diazepam was administered via gavage; the control rats received only saline injection. A wireless system was used for collecting EEG, EMG, and body temperature data within 72 h after propofol injection. After data collection, blood samples and hypothalamic tissue samples were collected for determining serum levels of oxidative stress markers and hypothalamic expressions of the key clock proteins.</p><p><strong>Results: </strong>Compared with the control rats, the rats with CBD treatment showed significantly increased sleep time at night (20:00-6:00), especially during the time period of 4:00-6:00 am. Compared with the rats in propofol group, which had prolonged SWS time and increased sleep episodes during 18:00-24:00 and sleep-wake transitions, the CBD-treated rats exhibited a significant reduction of SWS time and fewer SWS-to-active-awake transitions with increased SWS aspects and sleep-wake transitions at night (24:00-08:00). Diazepam treatment produced similar effect to CBD but with a weaker effect on sleep-wake transitions. Propofol caused significant changes in protein expressions and redox state, which were effectively reversed by CBD treatment.</p><p><strong>Conclusions: </strong>CBD can improve sleep structure and circadian rhythm in rats with propofol-induced sleep disorder possibly by regulating hypothalamic expressions of the key circadian clock proteins, suggesting a new treatment option for perioperative sleep disorders.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"744-750"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Hypertension exacerbates postoperative learning and memory impairment in rats possibly due to UCP2 downregulation-mediated mitochondrial dysfunction].","authors":"Luyu Liu, Maowei Gong, Guosong Liao, Weixing Zhao, Qiang Fu","doi":"10.12122/j.issn.1673-4254.2025.04.07","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.07","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the correlation of hypertension with postoperative cognitive dysfunction and its possible mechanism.</p><p><strong>Methods: </strong>Twelve-week-old spontaneously hypertensive rats (SHRs) and Wistar-Kyoto (WKY) rats were both randomized into control group and surgical group (<i>n=</i>8). In the latter group, the rats received carotid artery exposure surgery under sevoflurane anesthesia to establish models of postoperative learning and memory impairment. Postoperative cognitive function changes of the rats were evaluated using behavioral tests. The hippocampus of the rats were collected for determining ATP level and mitochondrial membrane potential (MMP) and for detecting expressions of UCP2 and astrocyte markers (GFAP and NOX4) using Western blotting and immunofluorescence staining. Serum levels of ROS, IL-6, IL-1β and TNF‑α were detected using ELISA. Nissl staining was used to examine hippocampal neuronal loss in the CA1 region.</p><p><strong>Results: </strong>The SHRs exhibited exacerbated learning and memory deficits following the surgery as shown by significantly reduced performance in novel object recognition tests and context-related and tone-related fear conditioning experiments. Compared with WKY rats, the SHRs had significantly decreased mitochondrial UCP2 expression and MMP in the hippocampus, increased hippocampal ATP level, and markedly increased serum levels of ROS and inflammatory factors, showing also increased activation of hippocampal astrocytes and microglia and reduced number of neurons positive for Nissl staining.</p><p><strong>Conclusions: </strong>Hypertension can exacerbate major postoperative learning and memory impairment in rats possibly as a result of UCP2-mediated mitochondrial dysfunction and oxidative stress damage, which further leads to astrocyte overactivation and neuronal damage.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"725-735"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[High expression of DTX2 promotes proliferation, invasion and epithelial-mesenchymal transition of oxaliplatin-resistant colorectal cancer cells].","authors":"Zhennan Ma, Fuquan Liu, Xuefeng Zhao, Xiaowei Zhang","doi":"10.12122/j.issn.1673-4254.2025.04.18","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.18","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the role of DTX2 in regulating biological behaviors of oxaliplatin-resistant colorectal cancer cells (CRC/OXA cells).</p><p><strong>Methods: </strong>CCK8 assay was used to determine the inhibition rate of oxaliplatin-treated CRC cells. A CRC/OXA cell line was constructed, in which DTX2 expression level was detected. The cells were transfected with a DTX2-shRNA plasmid or co-transfected with DTX2-shRNA and pcDNA-Notch2, and the changes in cell proliferation, migration and invasion ability were evaluated using plate cloning assay, scratch assay and Transwell invasion assay. The expression levels of Notch2, NICD and epithelial-mesenchymal transition (EMT) proteins of the transfected cells were detected with Western blotting. In a nude mouse model bearing SW620/OXA cell xenografts, the effects of DTX2 knockdown and Notch2 overexpression in the implanted cells on tumor growth and protein expressions were tested.</p><p><strong>Results: </strong>The IC₅₀ of oxaliplatin was 6.00 μmol/L in SW620 cells and 8.00 μmol/L in LoVo cells. CRC/OXA cells showed a significantly increased expression of DTX2. DTX2 knockdown in CRC/OXA cells significantly inhibited cell proliferation, migration and invasion, and these effects were reversed by co-transfection of the cells with pcDNA-Notch2. DTX2 knockdown significantly reduced the expression levels of Notch2, NICD and vimentin proteins and increased E-cadherin expression in CRC/OXA cells, and co-transfection with pcDNA-Notch2 potently attenuated the changes in these proteins. In the tumor-bearing mice, DTX2 overexpression obviously promoted the growth of SW620/OXA cell xenograft, enhanced the protein expressions of Notch2, NICD and vimentin, and lowered the expression of E-cadherin.</p><p><strong>Conclusions: </strong>High expression of DTX2 promotes proliferation, migration, invasion and EMT of CRC/OXA cells through the Notch2 signaling pathway, suggesting the potential of DTX2 as a target to improve the efficacy of oxaliplatin.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"829-836"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Early life <i>Bifidobacterium bifidum</i> BD-1 intervention alleviates hyperactivity of juvenile female rats with attention deficit hyperactivity disorder].","authors":"Yang Yang, Kai Wang, Jianxiu Liu, Zhimo Zhou, Wen Jia, Simou Wu, Jinxing Li, Fang He, Ruyue Cheng","doi":"10.12122/j.issn.1673-4254.2025.04.04","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.04","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effects of early life intervention with <i>Bifidobacterium bifidum</i> BD-1 (<i>B. bifidum</i> BD-1) on hyperactivity in a female mouse model of attention deficit hyperactivity disorder (ADHD) and explore the underlying mechanisms.</p><p><strong>Methods: </strong>Eight newborn female Wistar-Kyoto (WKY) rats and 6 spontaneous hypertensive rats (SHRs) were gavaged with saline and another 6 SHRs were gavaged with <i>B. bifidum</i> BD-1 (10⁹ CFU) daily for 3 weeks. Open field test of the rats was conducted at 7 weeks, and fecal samples were collected at weaning (3 weeks) and at 7 weeks for 16S rRNA sequencing. Immunofluorescent staining was used to detect dopamine transporter (DAT) and tyrosine hydroxylase (Th) levels in the striatum and activated microglia in the prefrontal cortex. Treg cells in the mesenteric lymph nodes, spleen and blood were analyzed using flow cytometry.</p><p><strong>Results: </strong>The SHRs traveled a significantly greater distance in open fields test than WKY rats, and this behavior was significantly attenuated by <i>B. bifidum</i> BD-1 intervention. The expression of DAT and Th in the striatum was significantly lower in the SHRs than in WKY rats, while <i>B. bifidum</i> BD-1 treatment obviously increased Th levels in the SHRs. <i>B. bifidum</i> BD-1 intervention significantly deceased the number of activated microglia and increased Treg cell counts in the spleen of SHRs. The treatment also enhanced α diversity in gut microbiota of the SHRs and resulted in a decreased <i>Firmicutes</i>/<i>Bacteroidota</i> ratio, more active <i>Muribaculaceae</i> growth, and suppression of <i>Clostridia_UCG-014</i> proliferation.</p><p><strong>Conclusions: </strong>Early life intervention with <i>B. bifidum</i> BD-1 alleviates hyperactivity in female SHRs by modulating the gut microbiota and peripheral immune response, suppressing neuroinflammation and improving dopaminergic system function. These findings provide evidence for early prevention strategies and support the development and application of psychobiotics for ADHD.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"702-710"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Activation of astrocytes in the dorsomedial hypothalamus accelerates sevoflurane anesthesia emergence in mice].","authors":"Shuting Guo, Fuyang Cao, Yongxin Guo, Yanxiang Li, Xinyu Hao, Zhuoning Zhang, Zhikang Zhou, Li Tong, Jiangbei Cao","doi":"10.12122/j.issn.1673-4254.2025.04.10","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.10","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the regulatory role of astrocytes in the dorsomedial hypothalamus (DMH) during sevoflurane anesthesia emergence.</p><p><strong>Methods: </strong>Forty-two male C57BL/6 mice were randomized into 6 groups (<i>n</i>=7) for assessing astrocyte activation in the dorsomedial hypothalamus (DMH) under sevoflurane anesthesia. Two groups of mice received microinjection of agfaABC1D promoter-driven AAV2 vector into the DMH for GCaMP6 overexpression, and the changes in astrocyte activity during sevoflurane or air inhalation were recorded using calcium imaging. For assessing optogenetic activation of astrocytes, another two groups of mice received microinjection of an optogenetic virus or a control vector into the DMH with optic fiber implantation, and sevoflurane anesthesia emergence was compared using behavioral experiments. In the remaining two groups, electroencephalogram (EEG) recording during sevoflurane anesthesia emergence was conducted after injection of the hChR2-expressing and control vectors. Anesthesia induction and recovery were assessed by observing the righting reflex. EEG data were recorded under 2.0% sevoflurane to calculate the burst suppression ratio (BSR) and under 1.5% sevoflurane for power spectrum analysis. Immunofluorescence staining was performed to visualize the colocalization of GFAP-positive astrocytes with viral protein signals.</p><p><strong>Results: </strong>Astrocyte activity in the DMH decreased progressively as sevoflurane concentration increased. During 2.0% sevoflurane anesthesia, the mice injected with the ChR2-expressing virus exhibited a significantly shortened wake-up time (<i>P</i><0.05), and optogenetic activation of the DMH astrocytes led to a marked reduction in BSR (<i>P</i><0.001). Under 1.5% sevoflurane anesthesia, optogenetic activation resulted in a significant increase in EEG gamma power and a significant decrease in delta power in ChR2 group (<i>P</i><0.01).</p><p><strong>Conclusions: </strong>Optogenetic activation of DMH astrocytes facilitates sevoflurane anesthesia emergence but does not significantly influence anesthesia induction. These findings offer new insights into the mechanisms underlying anesthesia emergence and may provide a potential target for accelerating postoperative recovery and managing anesthesia-related complications.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"751-759"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Causal relationship between autoimmune diseases and aplastic anemia: A Mendelian randomization study].","authors":"Wenjie Li, Yaonan Hong, Rui Huang, Yuchen Li, Ying Zhang, Yun Zhang, Dijiong Wu","doi":"10.12122/j.issn.1673-4254.2025.04.23","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.23","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the causal associations between autoimmune diseases and aplastic anemia (AA) using Mendelian randomization analysis.</p><p><strong>Methods: </strong>Publicly available genome-wide association study (GWAS) data were utilized to obtain single nucleotide polymorphisms (SNPs) associated with autoimmune diseases and AA for analysis. The inverse variance weighted (IVW) method was employed as the primary analytical approach, with MR Egger, Weighted Mode, Weighted Median, and Simple Mode methods serving as complementary analyses. Heterogeneity and pleiotropy analyses were conducted using designated functions, and the robustness of Mendelian randomization results was assessed using leave-one-out analysis.</p><p><strong>Results: </strong>The two-sample Mendelian randomization analysis using the IVW method revealed significant positive causal associations of rheumatoid arthritis (OR=1.094, 95% <i>CI</i>: 1.023-1.170, <i>P</i>=0.009, adjusted <i>P</i>=0.042), systemic lupus erythematosus (OR=1.111, 95% <i>CI</i>: 1.021-1.208, <i>P</i>=0.015, adjusted <i>P</i>=0.036), Hashimoto thyroiditis (OR=1.206, 95% <i>CI</i>: 1.049-1.387, <i>P</i>=0.009, adjusted <i>P</i>=0.029), and Sicca syndrome (OR=1.173, 95% <i>CI</i>: 1.054-1.306, <i>P</i>=0.004, adjusted <i>P</i>=0.035) with AA, which was supported by the results from the Weighted Median method. Sensitivity analyses indicated no evidence of pleiotropy or heterogeneity, and leave-one-out analysis confirmed the robustness of the causal relationships. No direct evidence was found linking Graves' disease, ulcerative colitis, Crohn's disease, autoimmune hepatitis, primary biliary cholangitis, or primary sclerosing cholangitis with AA (<i>P</i>>0.05, adjusted <i>P</i>>0.05), indicating a lack of causal association. Reverse Mendelian randomization results and multiple corrections indicated that AA was not an influencing factor for autoimmune diseases (adjusted <i>P</i>>0.05).</p><p><strong>Conclusions: </strong>Our findings support at the genetic level that rheumatoid arthritis, systemic lupus erythematosus, Hashimoto thyroiditis, and Sicca syndrome are risk factors for AA, and confirm a causal association of the these 4 autoimmune diseases with an increased risk of AA.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"871-879"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A low-dose CT image restoration method based on central guidance and alternating optimization].","authors":"Xiaoyu Zhang, Hao Wang, Dong Zeng, Zhaoying Bian","doi":"10.12122/j.issn.1673-4254.2025.04.20","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.20","url":null,"abstract":"<p><strong>Objectives: </strong>We propose a low-dose CT image restoration method based on central guidance and alternating optimization (FedGP).</p><p><strong>Methods: </strong>The FedGP framework revolutionizes the traditional federated learning model by adopting a structure without a fixed central server, where each institution alternatively serves as the central server. This method uses an institution-modulated CT image restoration network as the core of client-side local training. Through a federated learning approach of central guidance and alternating optimization, the central server leverages local labeled data to guide client-side network training to enhance the generalization capability of the CT imaging model across multiple institutions.</p><p><strong>Results: </strong>In the low-dose and sparse-view CT image restoration tasks, the FedGP method showed significant advantages in both visual and quantitative evaluation and achieved the highest PSNR (40.25 and 38.84), the highest SSIM (0.95 and 0.92), and the lowest RMSE (2.39 and 2.56). Ablation study of FedGP demonstrated that compared with FedGP(w/o GP) without central guidance, the FedGP method better adapted to data heterogeneity across institutions, thus ensuring robustness and generalization capability of the model in different imaging conditions.</p><p><strong>Conclusions: </strong>FedGP provides a more flexible FL framework to solve the problem of CT imaging heterogeneity and well adapts to multi-institutional data characteristics to improve generalization ability of the model under diverse imaging geometric configurations.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"844-852"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Aurora-A overexpression promotes cervical cancer cell invasion and metastasis by activating the NF-κBp65/ARPC4 signaling axis].","authors":"Yaqing Yue, Zhaoxia Mu, Xibo Wang, Yan Liu","doi":"10.12122/j.issn.1673-4254.2025.04.19","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.19","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the regulatory effects of Aurora-A in regulating proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of cervical cancer cells and the role of actin-related protein 2/3 complex subunit 4 (ARPC4) in mediating its effects.</p><p><strong>Methods: </strong>The plasmids pCDH-NC, pCDH-Aurora-A, and shRNA-ARPC4 were used for inducing Aurora-A overexpression or ARPC4 knockdown in HeLa cells. The cells were divided into vector group, Aurora-A overexpression group, Aurora-A overexpression+ARPC4 knockdown group, and Aurora-A overexpression+NF‑κBp65 inhibitor group and transfected with the corresponding plasmids. The proliferation, colony-forming ability, migration and invasion of the treated Hela cells was evaluated using EdU immunofluorescence assay, crystal violet staining, scratch assay, Transwell assay, and Matrigel assay. Western blotting was performed to detect the changes in cellular expressions of EMT-related proteins and expression levels of NF-κBp65 and ARPC4.</p><p><strong>Results: </strong>The expression of ARPC4 was significantly decreased in HeLa cells with Aurora-A knockdown and increased in Aurora-A-overexpressing cells. Aurora-A overexpression obviously promoted proliferation, migration, and invasion abilities of HeLa cells, and these effects was significantly antagonized by ARPC4 knockdown. In Aurora-A-overexpressing cells, the phosphorylation level of NF-κBp65 and the expression level of ARPC4 were increased significantly, and application of the NF‑κBp65 inhibitor obviously lowered the expression level of ARPC4.</p><p><strong>Conclusions: </strong>Aurora-A overexpression upregulates the expression of ARPC4 by activating the NF-κBp65 signaling pathway, thereby promoting migration, invasion and EMT of HeLa cells.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"837-843"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Risk factors for overall postoperative complications in elderly patients undergoing gastrointestinal surgeries: a multicenter observational study].","authors":"Xuecai Lü, Yanhong Liu, Shiyi Han, Haoyun Zhang, Aisheng Hou, Zhikang Zhou, Likai Shi, Jie Gao, Jiangbei Cao, Hong Zhang, Weidong Mi","doi":"10.12122/j.issn.1673-4254.2025.04.08","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.08","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the risk factors of overall postoperative complications in elderly patients undergoing gastrointestinal surgeries.</p><p><strong>Methods: </strong>This study was conducted among a total of 1388 elderly patients, who underwent elective gastrointestinal surgeries at 17 centers across China between April, 2020 and April, 2022. The primary outcome was the incidence of postoperative complications within 30 days, including procedure-related, neuropsychiatric, respiratory, cardiovascular, and gastrointestinal complications as well as acute kidney injury. Baseline characteristics, preoperative psychological and functional status, intraoperative anesthesia and surgical factors, intraoperative medication, use of nerve block, and postoperative analgesia methods were compared between the patients experiencing one or more postoperative complications and those without complications. Univariate and multivariate logistic regression analyses were performed to identify the independent risk factors for postoperative complications. The relationship between postoperative acute pain and each type of complication were explored.</p><p><strong>Results: </strong>The incidence of overall postoperative complications was 50.8% (705/1388) in these patients. Multivariate analysis showed that age (OR: 1.026; 95% <i>CI</i>: 1.006-1.046), prognostic nutritional index (OR: 0.998; 95% <i>CI</i>: 0.997-1.000), preoperative EuroQol-5 dimensions score (OR: 0.094; 95% <i>CI</i>: 0.018-0.500), blood loss (OR: 1.002; 95% <i>CI</i>: 1.001-1.003), and acute postoperative pain (OR: 1.308; 95% <i>CI</i>: 1.033-1.657) were significantly associated with the occurrence of postoperative complications. Specifically, patients experiencing severe postoperative pain had a significantly higher incidence of neuropsychiatric (27.2% <i>vs</i> 19.8%), procedure-related (17.3% <i>vs</i> 10.2%), and cardiovascular complications (3.6% <i>vs</i> 1.7%).</p><p><strong>Conclusions: </strong>An advanced age, a low preoperative nutritional index, a poor quality of life score, a greater volume of intraoperative blood loss, and acute postoperative pain are independent risk factors for postoperative complications in elderly patients undergoing gastrointestinal surgeries. There is a significant association between acute postoperative pain and multi-system complications.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"736-743"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[High expression of CDKN3 promotes migration and invasion of gastric cancer cells by regulating the p53/NF-κB signaling pathway and inhibiting cell apoptosis].","authors":"Yi Zhang, Yu Shen, Zhiqiang Wan, Song Tao, Yakui Liu, Shuanhu Wang","doi":"10.12122/j.issn.1673-4254.2025.04.21","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.21","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the expression of CDKN3 in gastric cancer and its impact on prognosis of gastric cancer patients.</p><p><strong>Methods: </strong>We analyzed CDKN3 expression in clinical specimens from 114 gastric cancer patients and assessed its association with 5-year postoperative survival of the patients. GO and KEGG enrichment analyses were used to predict the biological function and possible mechanism of CDKN3. The effects of lentivirus-mediated CDKN3 knockdown on biological behaviors of gastric cancer cells were evaluated using Transwell assay, CCK-8 assay, TUNEL staining, flow cytometry, and Western blotting.</p><p><strong>Results: </strong>CDKN3 expression was significantly higher in gastric cancer tissues than in the adjacent tissues with significant correlations with CEA level, CA19-9 level, and T and N staging (<i>P</i><0.05). High CDKN3 expression was an independent risk factor affecting 5-year postoperative survival of the patients and predictive for long-term prognosis (<i>P</i><0.01). Enrichment analyses suggested a probable association of CDKN3 with apoptosis. In MGC-803 cells, CDKN3 knockdown significantly lowered migration and invasion capacities of the cells, while CDKN3 overexpression produced the opposite effects. TUNEL staining revealed a significantly lower level of cell apoptosis in gastric cancer tissues than in adjacent tissues (<i>P</i><0.01). CDKN3 knockdown obviously inhibited proliferation and increased apoptosis of MGC-803 cells. CDKN3 overexpression down-regulated the expressions of p53, p21 and Bax and up-regulated the expressions of p-p65 and Bcl-2.</p><p><strong>Conclusions: </strong>CDKN3 is highly expressed in gastric cancer tissues and affects patient prognosis. CDKN3 overexpression promotes proliferation, invasion and migration and suppressed apoptosis of gastric cancer cells possibly through the p53/NF-κB signaling pathway.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"853-861"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}