南方医科大学学报杂志最新文献

{"title":"<i>Pulsatilla saponin D</i> inhibits invasion and metastasis of triple-negative breast cancer cells through multiple targets and pathways.","authors":"Qiao Chu, Xiaona Wang, Jiaying Xu, Huilin Peng, Yulin Zhao, Jing Zhang, Guoyu Lu, Kai Wang","doi":"10.12122/j.issn.1673-4254.2025.01.18","DOIUrl":"10.12122/j.issn.1673-4254.2025.01.18","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the mechanism by which <i>Pulsatilla saponin D</i> (PSD) inhibits invasion and metastasis of triple-negative breast cancer (TNBC).</p><p><strong>Methods: </strong>The public databases were used to identify the potential targets of PSD and the invasion and metastasis targets of TNBC to obtain the intersection targets between PSD and TNBC. The \"PSD-target-disease\" interaction network was constructed and protein-protein interaction (PPI) analysis was performed to obtain the core targets, which were analyzed for KEGG pathway and GO functional enrichment. Molecular docking study of the core targets and PSD was performed, and the therapeutic effect and mechanism of PSD were verified using Transwell assay and Western blotting in cultured TNBC cells.</p><p><strong>Results: </strong>Network pharmacology analysis identified a total of 285 potential PSD targets and 26 drug-disease intersection core targets. GO analysis yielded 175 entries related to the binding of biomolecules (protein, DNA and RNA), enzyme activities, and regulation of gene transcription. KEGG analysis yielded 46 entries involving pathways in cancer, chemical carcinogenesis-receptor activation, microRNAs in cancer, chemical carcinogenesis-reactive oxygen species, PD-L1 expression and PD-1 checkpoint pathway in cancer. Molecular docking showed high binding affinities of PSD to MTOR, HDAC2, ABL1, CDK1, TLR4, TERT, PIK3R1, NFE2L2 and PTPN1. In cultured TNBC cells, treatment with PSD significantly inhibited cell invasion and migration and lowered the expressions of MMP2, MMP9, N-cadherin and the core proteins p-mTOR, ABL1, TERT, PTPN1, HDAC2, PIK3R1, CDK1, TLR4 as well as NFE2L2 expressionin the cell nuclei.</p><p><strong>Conclusions: </strong>The inhibitory effects of PSD on TNBC invasion and metastasis are mediated by multiple targets and pathways.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 1","pages":"150-161"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin improves heart failure by inhibiting cardiomyocyte apoptosis <i>via</i> suppressing the MAPK signaling pathway.","authors":"Xiupeng Long, Shun Tao, Shen Yang, Suyun Li, Libing Rao, Li Li, Zhe Zhang","doi":"10.12122/j.issn.1673-4254.2025.01.22","DOIUrl":"10.12122/j.issn.1673-4254.2025.01.22","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the mechanism that mediate the therapeutic effect of quercetin on heart failure.</p><p><strong>Methods: </strong>We searched the TCMSP and Swiss ADME databases for the therapeutic targets of quercetin and retrieved heart failure targets from the Genecards and OMIM databases. The intersecting targets were analyzed with GO and KEGG pathway analysis using DAVID database, and the key genes were identified <i>via</i> PPI analysis. Molecular docking between the core targets and quercetin was performed using PyMOL and AutoDock Tools. In a heart failure model established in H9C2 cardiomyocytes by treatment with isoproterenol, the effect of quercetin on the expressions of the MAPK signaling pathway was tested.</p><p><strong>Results: </strong>A total of 60 intersecting targets were identified. Enrichment analysis revealed that quercetin may inhibit heart failure through the MAPK signaling pathway. The core genes, including AMPK3 and BCL-2, were identified as potential key regulators in quercetin-mediated improvement of heart failure. Cellular experiments demonstrated that quercetin significantly reduced isoproterenol-induced apoptosis of cardiomyocytes in a dose-dependent manner and obviously decreased the Bax/Bcl-2 ratio and the expression levels of caspase-3, ERK and p38 in the cells.</p><p><strong>Conclusions: </strong>Quercetin improves heart failure possibly by inhibiting cardiomyocyte apoptosis through the MAPK signaling pathway.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 1","pages":"187-196"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NLRP6 overexpression improves nonalcoholic fatty liver disease by promoting lipid oxidation and decomposition in hepatocytes through the AMPK/CPT1A/PGC1A pathway.","authors":"Qing Shi, Suye Ran, Lingyu Song, Hong Yang, Wenjuan Wang, Hanlin Liu, Qi Liu","doi":"10.12122/j.issn.1673-4254.2025.01.15","DOIUrl":"10.12122/j.issn.1673-4254.2025.01.15","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the regulatory role of nucleotide-bound oligomerized domain-like receptor containing pyrin-domain protein 6 (NLRP6) in liver lipid metabolism and non-alcoholic fatty liver disease (NAFLD).</p><p><strong>Methods: </strong>Mouse models with high-fat diet (HFD) feeding for 16 weeks (<i>n</i>=6) or with methionine choline-deficient diet (MCD) feeding for 8 weeks (<i>n</i>=6) were examined for the development of NAFLD using HE and oil red O staining, and hepatic expressions of NLRP6 were detected with RT-qPCR, Western blotting, and immunohistochemical staining. Cultured human hepatocytes (LO2 cells) with adenovirus-mediated NLRP6 overexpression or knock-down were treated with palmitic acid (PA) in the presence or absence of compound C (an AMPK inhibitor), and the changes in cellular lipid metabolism were examined by measuring triglyceride, ATP and β-hydroxybutyrate levels and using oil red staining, RT-qPCR, and Western blotting.</p><p><strong>Results: </strong>HFD and MCD feeding both resulted in the development of NAFLD in mice, which showed significantly decreased NLRP6 expression in the liver. In PA-treated LO2 cells, NLRP6 overexpression significantly decreased cellular TG content and lipid deposition, while NLRP6 knockdown caused the opposite effects. NLRP6 overexpression in PA-treated LO2 cells also increased mRNA and protein expressions of PGC1A and CPT1A, levels of ATP and β-hydroxybutyrate, and the phosphorylation level of AMPK pathway; the oxidative decomposition of lipids induced by Ad-NLRP6 was inhibited by the use of AMPK inhibitors.</p><p><strong>Conclusions: </strong>NLRP6 overexpression promotes lipid oxidation and decomposition through AMPK/CPT1A/PGC1A to alleviate lipid deposition in hepatocytes.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 1","pages":"118-125"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of CHMP2B suppresses proliferation of renal clear cell carcinoma cells.","authors":"Xiaorui Chen, Qingzheng Wei, Zongliang Zhang, Jiangshui Yuan, Weiqing Song","doi":"10.12122/j.issn.1673-4254.2025.01.16","DOIUrl":"10.12122/j.issn.1673-4254.2025.01.16","url":null,"abstract":"<p><strong>Objectives: </strong>To analyze the association of CHMP2B expression level of with clinicopathological characteristics and prognosis of clear cell renal cell carcinoma (CRCC) and the possible role of CHMP2B in tumorigenesis and progression of CRCC.</p><p><strong>Methods: </strong>RNAseq data of CRCC were downloaded from the TCGA database for analysis of CHMP2B expression levels in tumor and adjacent tissues and their correlation with clinicopathological characteristics of the patients. Survival outcomes of the patients with high and low CHMP2B expressions were analyzed using the Kaplan-Meier model, and the COX risk regression model was used for identifying the prognostic factors of the patients. The correlation between CHMP2B expression and immune infiltration, its co-expressed genes, and the effect of CHMP2B gene mutations on immunotherapy responses, and its immunohistochemical expression in CRCC and normal tissues were analyzed. Clinical samples of CRCC were collected to examine CHMP2B expressions using RT-PCR, and cell experiment was carried out to test the effect of CHMP2B overexpression on biological behaviors of CRCC cells.</p><p><strong>Results: </strong>CHMP2B was significantly under-expressed in renal cancer tissues, and its overexpression obviously inhibited the proliferation of CRCC cells <i>in vitro</i>. CHMP2B expression level was significantly correlated with age, gender, lymph node metastasis, and tumor stage, and the patients with low CHMP2B expression had poor survival outcomes. Enrichment and co-expression gene analyses suggested that CHMP2B was mainly involved in viral outgrowth, necrotic apoptosis, endocytosis, and immune-regulatory processes in kidney cancer.</p><p><strong>Conclusions: </strong>CHMP2B is lowly expressed in renal cancer tissues to affect tumor progression and tumor immune processes, and may serve as a prognostic biomarker and therapeutic target for CRCC.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 1","pages":"126-136"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential expressions of exosomal miRNAs in patients with chronic heart failure and hyperuricemia: diagnostic values of miR-27a-5p and miR-139-3p.","authors":"Zhiliang Chen, Yonggang Yang, Xia Huang, Yan Cheng, Yuan Qu, Qiqi Heng, Yujia Fu, Kewei Li, Ning Gu","doi":"10.12122/j.issn.1673-4254.2025.01.06","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.01.06","url":null,"abstract":"<p><strong>Objectives: </strong>To analyze the differentially expressed exosomal miRNAs in patients with chronic heart failure (CHF) complicated by hyperuricemia (HUA) and explore their potential as novel diagnostic molecular markers and their target genes.</p><p><strong>Methods: </strong>This study was conducted among 30 CHF patients with HUA (observation group) and 30 healthy volunteers (control group) enrolled between September, 2020 and September, 2023. Peripheral blood samples were collected from 6 CHF patients with HUA for analyzing exosomal miRNAs by high-throughput sequencing, and the results were validated in the remaining 24 patients using qRT-PCR. GO and KEGG enrichment analyses were performed to predict the the target genes of the identified differential miRNAs. We also validated the differentially expressed miRNAs by animal experiment.</p><p><strong>Results: </strong>A total of 42 differentially expressed exosomal miRNAs were detected in observation group by high-throughput sequencing; among them, miR-27a-5p was significantly upregulated (<i>P</i>=0.000179), and miR-139-3p was significantly downregulated (<i>P</i>=0.000058). In the 24 patients with both CHF and PUA, qRT-PCR validated significant upregulation of miR-27a-5p (<i>P</i>=0.004) and downregulation of miR-139-3p (<i>P</i>=0.005) in serum exosomes. When combined, miR-27a-5p and miR-139-3p had a maximum area under the curve (AUC) of 0.899 (95% <i>CI</i>: 0812-0.987) for predicting CHF complicated by HUA. GO and KEGG enrichment analyses suggested that the differential expressions of miR-27a-5p and miR-139-3p was associated with the activation of the AMPK-mTOR signaling pathway to activate the autophagic response. We obtained the same conclusion from animal experiment.</p><p><strong>Conclusions: </strong>Upregulated exosomal miR-27a-5p combined with downregulated exosomal miR-139-3p expression can serve as a novel molecular marker for diagnosis of CHF complicated by HUA, and their differential expression may promote autophagy in cardiomyocytes by activating the AMPK-mTOR signaling pathway.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 1","pages":"43-51"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Schistosoma japonicum</i> cystatin has protective effects against \"two-hit\" sepsis in mice by regulating the inflammatory microenvironment.","authors":"Wenjuan Duo, Yixiang Wang, Jiaxing Wang, Xinlong Xu, Linxian Li, Dongchen Yang, Qili Shen, Lichun Yang, Xiaojing Liu, Qiwang Jing, Liang Chu, Xiaodi Yang","doi":"10.12122/j.issn.1673-4254.2025.01.14","DOIUrl":"10.12122/j.issn.1673-4254.2025.01.14","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the protective effect of <i>Schistosoma japonicum</i> cystatin (r<i>Sj</i>-Cystatin) in a mouse mode of \"two-hit\" sepsis.</p><p><strong>Methods: </strong>Sixty male C57BL/6 mice randomized equally into sham-operated group, protein group, \"two-hit\" modeling group, and protein intervention group. In the former two groups, the mice received an intraperitoneal injection of 100 μL PBS followed by exposure of the cecum and then by intraperitoneal injection of 100 μL PBS or 25 μg r<i>Sj</i>-Cystatin 30 min later; In the latter two groups, 100 μL PBS containing LPS (5 mg/kg) was injected intraperitoneally 24 h before cecal ligation and puncture (CLP), and 100 μL PBS or 25 μg r<i>Sj</i>-Cystatin were injected 30 min after CLP. At 12 h after r<i>Sj</i>-Cystatin treatment, 6 mice from each group were sacrificed for detection of TNF-α, IL-6, IL-10, TGF-β, iNOS and Arg-1 in the serum, spleen, liver, lung and kidney tissues using ELISA, for examinations of liver, lung and kidney pathologies with HE staining, and for analysis of CD3⁺CD4⁺CD25⁺Foxp3⁺ T cell percentage in the spleen using flow cytometry. The remaining mice were observed for general condition and 72-h survival.</p><p><strong>Results: </strong>The 72-h survival rates in the 4 groups were 100%, 100%, 0% and 20%, respectively, showing significant differences between the latter two groups. The mouse models of \"two-hit\" sepsis exhibited obvious tissue pathologies and significant elevations of TNF-α and IL-6 in both the serum and tissue homogenate, which were significantly ameliorated by r<i>Sj</i>-Cystatin treatment. Treatment with r<i>Sj</i>-Cystatin also increased IL-10 and TGF-β levels and spleen CD3⁺CD4⁺CD25⁺Foxp3⁺ T cell percentage. The septic mouse models also showed increased iNOS levels in all the detected tissues and a decreased Arg-1 level in the kidney, and these changes were obviously improved by r<i>Sj</i>-Cystatin treatment.</p><p><strong>Conclusions: </strong>r<i>Sj</i>-Cystatin has a protective effect against \"two-hit\" sepsis in mice by regulating the inflammatory microenvironment.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 1","pages":"110-117"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between the Observer's Assessment of Alertness/Sedation score and bispectral index in patients receiving propofol titration during general anesthesia induction.","authors":"Lihong Chen, Huilin Xie, Xia Huang, Tongfeng Luo, Jing Guo, Chunmeng Lin, Xueyan Liu, Lishuo Shi, Sanqing Jin","doi":"10.12122/j.issn.1673-4254.2025.01.07","DOIUrl":"10.12122/j.issn.1673-4254.2025.01.07","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the relationship between the Observer's Assessment of Alertness/Sedation (OAAS) score and the bispectral index (BIS) during propofol titration for general anesthesia induction and analyze the impact of BIS monitoring delay on anesthetic depth assessment.</p><p><strong>Methods: </strong>This study was conducted among 90 patients (ASA class I-II) undergoing elective surgery under general anesthesia. For anesthesia induction, the patients received propofol titration at the rate of 0.5 mg·kg^-1·min^-1 till OAAS scores of 4, 3, 2, and 1 were reached. After achieving an OAAS score of 1, remifentanil (2 μg·kg⁻¹) and rocuronium (0.6 mg·kg⁻¹) were administered, and tracheal intubation was performed 2 min later. BIS values, mean arterial pressure (MAP), heart rate (HR), and propofol dosage at each OAAS score were recorded, and the correlation between OAAS scores and BIS values was analyzed. The diagnostic performance of BIS values for determining when the OAAS score reaches 1 was analyzed using ROC curve.</p><p><strong>Results: </strong>All the patients successfully completed tracheal intubation. BIS values of the patients at each of the OAAS scores differed significantly (<i>P</i><0.01), and the mean BIS value decreased by 4.08, 8.32, 5.43 and 5.24 as the OAAS score decreased from 5 to 4, from 4 to 3, from 3 to 2, and from 2 to 1, respectively. There was a significant correlation between the OAAS score and BIS values (<i>ρ</i>=0.775, <i>P</i><0.001). The median BIS value for an OAAS score of 1 was 76, at which point 83.33% of the patients had BIS values exceeding 60. ROC curve analysis showed that for determining an OAAS score of 1, BIS value, at the optimal cutoff value of 84, had a sensitivity of 88.9%, a specificity of 73.3%, and an area under the curve of 0.842 (0.803-0.881).</p><p><strong>Conclusions: </strong>OAAS score during induction of general anesthesia is strongly correlated with BIS value and is a highly sensitive and timely indicator to compensate for the delay in BIS monitoring.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 1","pages":"52-58"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fusion model of manually extracted visual features and deep learning features for rebleeding risk stratification in peptic ulcers.","authors":"Peishan Zhou, Wei Yang, Qingyuan Li, Xiaofang Guo, Rong Fu, Side Liu","doi":"10.12122/j.issn.1673-4254.2025.01.23","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.01.23","url":null,"abstract":"<p><strong>Objectives: </strong>We propose a multi-feature fusion model based on manually extracted features and deep learning features from endoscopic images for grading rebleeding risk of peptic ulcers.</p><p><strong>Methods: </strong>Based on the endoscopic appearance of peptic ulcers, color features were extracted to distinguish active bleeding (Forrest I) from non-bleeding ulcers (Forrest II and III). The edge and texture features were used to describe the morphology and appearance of the ulcers in different grades. By integrating deep features extracted from a deep learning network with manually extracted visual features, a multi-feature representation of endoscopic images was created to predict the risk of rebleeding of peptic ulcers.</p><p><strong>Results: </strong>In a dataset consisting of 3573 images from 708 patients with Forrest classification, the proposed multi-feature fusion model achieved an accuracy of 74.94% in the 6-level rebleeding risk classification task, outperforming the experienced physicians who had a classification accuracy of 59.9% (<i>P</i><0.05). The F1 scores of the model for identifying Forrest Ib, IIa, and III ulcers were 90.16%, 75.44%, and 77.13%, respectively, demonstrating particularly good performance of the model for Forrest Ib ulcers. Compared with the first model for peptic ulcer rebleeding classification, the proposed model had improved F1 scores by 5.8%. In the simplified 3-level risk (high-risk, low-risk, and non-endoscopic treatment) classification task, the model achieved F1 scores of 93.74%, 81.30%, and 73.59%, respectively.</p><p><strong>Conclusions: </strong>The proposed multi-feature fusion model integrating deep features from CNNs with manually extracted visual features effectively improves the accuracy of rebleeding risk classification for peptic ulcers, thus providing an efficient diagnostic tool for clinical assessment of rebleeding risks of peptic ulcers.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 1","pages":"197-205"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quercetin mediates the therapeutic effect of <i>Centella asiatica</i> on psoriasis by regulating STAT3 phosphorylation to inhibit the IL-23/IL-17A axis.","authors":"Qing Liu, Jing Liu, Yihang Zheng, Jin Lei, Jianhua Huang, Siyu Liu, Fang Liu, Qunlong Peng, Yuanfang Zhang, Junjie Wang, Yujuan Li","doi":"10.12122/j.issn.1673-4254.2025.01.12","DOIUrl":"10.12122/j.issn.1673-4254.2025.01.12","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the active components that mediate the therapeutic effect of <i>Centella asiatica</i> on psoriasis and their therapeutic mechanisms.</p><p><strong>Methods: </strong>TCMSP, TCMIP, PharmMapper, Swiss Target Prediction, GeneCards, OMIM and TTD databases were searched for the compounds in <i>Centella asiatica</i> and their targets and the disease targets of psoriasis. A drug-active component-target network and the protein-protein interaction network were constructed, and DAVID database was used for pathway enrichment analysis. In a RAW264.7 macrophage model of LPS-induced inflammation, the anti-inflammatory effect of 7.5, 15, 30, and 60 μmol/L quercetin, asiaticoside, and asiatic acid, which were identified as the main active components in <i>Centella asiatica</i>, were tested by measuring cellular production of NO, TNF‑α and IL-6 using Griess method and ELISA and by detecting mRNA expressions of IL-23, IL-17A, TNF-α and IL-6 and protein expressions of p-STAT3 (Tyr705) and p-STAT3 (Ser727) with RT-qPCR and Western blotting.</p><p><strong>Results: </strong>A total of 139 targets of <i>Centella asiatica</i> and 4604 targets of psoriasis were obtained, and among them CASP3, EGFR, PTGS2, and ESR1 were identified as the core targets. KEGG analysis suggested that quercetin, asiaticoside, and asiatic acid in <i>Centella asiatica</i> were involved in cancer and IL-17 and MAPK signaling pathways. In the RAW264.7 macrophage model of inflammation, treatment with quercetin significantly reduced cellular production of NO, TNF‑α and IL-6, and lowered mRNA expressions of IL-23, IL-17A, TNF‑α and IL-6 and protein expressions of p-STAT3 (Tyr705) and p-STAT3 (Ser727).</p><p><strong>Conclusions: </strong>Quercetin, asiaticoside and asiatic acid are the main active components in <i>Centella asiatica</i> to mediate the therapeutic effect against psoriasis, and quercetin in particular is capable of suppressing cellular production of NO, TNF‑α and IL-6 and regulating the IL-23/IL-17A inflammatory axis by mediating STAT3 phosphorylation to inhibit inflammatory response.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 1","pages":"90-99"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A low-dose CT reconstruction method using sub-pixel anisotropic diffusion.","authors":"Shizhou Tang, Ruolan Su, Shuting Li, Zhenzhen Lai, Jinhong Huang, Shanzhou Niu","doi":"10.12122/j.issn.1673-4254.2025.01.19","DOIUrl":"10.12122/j.issn.1673-4254.2025.01.19","url":null,"abstract":"<p><strong>Objectives: </strong>We present a new low-dose CT reconstruction method using sub-pixel and anisotropic diffusion.</p><p><strong>Methods: </strong>The sub-pixel intensity values and their second-order differences were obtained using linear interpolation techniques, and the new gradient information was then embedded into an anisotropic diffusion process, which was introduced into a penalty-weighted least squares model to reduce the noise in low-dose CT projection data. The high-quality CT image was finally reconstructed using the classical filtered back-projection (FBP) algorithm from the estimated data.</p><p><strong>Results: </strong>In the Shepp-Logan phantom experiments, the structural similarity (SSIM) index of the CT image reconstructed by the proposed algorithm, as compared with FBP, PWLS-Gibbs and PWLS-TV algorithms, was increased by 28.13%, 5.49%, and 0.91%, the feature similarity (FSIM) index was increased by 21.08%, 1.78%, and 1.36%, and the root mean square error (RMSE) was reduced by 69.59%, 18.96%, and 3.90%, respectively. In the digital XCAT phantom experiments, the SSIM index of the CT image reconstructed by the proposed algorithm, as compared with FBP, PWLS-Gibbs and PWLS-TV algorithms, was increased by 14.24%, 1.43% and 7.89%, the FSIM index was increased by 9.61%, 1.78% and 5.66%, and the RMSE was reduced by 26.88%, 9.41% and 18.39%, respectively. In clinical experiments, the SSIM index of the image reconstructed using the proposed algorithm was increased by 19.24%, 15.63% and 3.68%, the FSIM index was increased by 4.30%, 2.92% and 0.43%, and the RMSE was reduced by 44.60%, 36.84% and 15.22% in comparison with FBP, PWLS-Gibbs and PWLS-TV algorithms, respectively.</p><p><strong>Conclusions: </strong>The proposed method can effectively reduce the noises and artifacts while maintaining the structural details in low-dose CT images.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 1","pages":"162-169"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}