南方医科大学学报杂志最新文献

{"title":"[<i>Xiaoyao San</i> alleviates abnormal α‑syn aggregation and neuronal injury in a <i>Caenorhabditis</i> elegans model of Parkinson disease <i>via</i> the SKN-1 antioxidative pathway].","authors":"Lixuan Ye, Yuxin Liao, Qiong Liu, Jia Zhang, Lei Gao, Zhaoyang Dong","doi":"10.12122/j.issn.1673-4254.2026.09.03","DOIUrl":"10.12122/j.issn.1673-4254.2026.09.03","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the neuroprotective effects of <i>Xiaoyao</i><i>San</i> (XYS) in a <i>Caenorhabditis elegans</i> (<i>C. elegans</i>) model of Parkinson disease (PD) and explore its molecular mechanism.</p><p><strong>Methods: </strong>A PD model was established in <i>C. elegans</i> using 6-hydroxydopamine (6-OHDA) and validated using a transgenic strain overexpressing α‑synuclein (α‑syn) (NL5901). The worms were treated with 25, 50 or 100 μg/mL XYS, and their lifespan was recorded. Their motor function and food-related behaviors were evaluated by counting body bends within 20 s and observing the basal slowing response. Dopaminergic neuron degeneration and α‑syn aggregation were observed in GFP-labeled transgenic worms. Intracellular ROS levels were measured using a DHE fluorescent probe, and the mRNA expressions of <i>dat-1</i>, <i>cat-2</i>, <i>ced-9</i>, <i>gst-4</i>, and <i>sod-3</i> were analyzed using RT-qPCR. RNA interference (RNAi) was employed to knock down skn-1 expression to determine whether the effects of XYS depend on the SKN-1/Nrf2 signaling pathway.</p><p><strong>Results: </strong>XYS significantly prolonged the lifespan of PD model worms, increased their body bends within 20 s on day 5 and day 10, and improved the basal slowing response. XYS obviously alleviated 6-OHDA-induced dopaminergic neuronal damage, upregulated the expression of <i>dat-1</i>, <i>cat-2</i>, and <i>ced-9</i>, and reduced α‑syn accumulation. Treatment with XYS decreased reactive oxygen species (ROS) levels and upregulated the antioxidant-related genes including <i>sod-3</i> and <i>gst-4</i>. Mechanistic studies showed that XYS significantly enhanced <i>skn-1</i>::GFP and <i>sod-3</i>::GFP fluorescence signals. Knocking down <i>skn-1</i> resulted in decreased lipid content in the worms, and XYS failed to significantly reverse this change; <i>skn-1</i> knockdown also abolished the restorative effect of XYS on <i>sod-3</i>::GFP fluorescence reduction.</p><p><strong>Conclusions: </strong>XYS improves neurological function in <i>Caenorhabditis elegans</i> PD model by alleviating oxidative stress and suppressing abnormal α‑syn aggregation, and its neuroprotective effect is mediated probably by the SKN-1/Nrf2-related antioxidant pathway.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 9","pages":"2023-2034"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13625000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[CHOP promotes hepatocyte pyroptosis by downregulating endoplasmic reticulophagy and enhancing endoplasmic reticulum-mitochondria interaction].","authors":"Xiaoyu Guo, Jiayu Wu, Bing Han, Ting Yang, Huanhuan Yin, Jinyi Xiong, Rujia Xie","doi":"10.12122/j.issn.1673-4254.2026.07.18","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.18","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the role of C/EBP homologous protein (CHOP) in dithiothreitol (DTT)‑induced endoplasmic reticulophagy in murine hepatocytes cells and its mechanism for regulating hepatocytes pyroptosis through endoplasmic reticulum (ER)‑mitochondria interactions.</p><p><strong>Methods: </strong>The changes in CHOP, endoplasmic reticulophagy, mitochondrial-ER contact sites (MERCs)-associated protein, and pyroptosis-associated proteins in DTT-treated BRL-3A cells were examined using Western blotting, and CHOP enrichment in the promoter regions of ATG5, ATG12 and LC3 genes was detected using ChIP assay. The effect of bafilomycin A1 (an autophagy inhibitor) pretreatment on DTT-induced BRL-3A cell death was assessed using Hoechst 33342 and propidium iodide staining. The effects of lentivirus-mediated CHOP overexpression in the hepatocytes on ER-phagy, MERCs-associated proteins, pyroptosis-associated proteins, and colocalizations of ER with the autophagosomes, lysosomes and mitochondria were examined using Western blotting and immunofluorescence double staining; the changes in intramitochondrial Ca²⁺, mitochondrial membrane potential and intramitochondrial GSDMD-N levels were also analyzed.</p><p><strong>Results: </strong>DTT treatment for 12 h did not significantly affect CHOP protein expression but significantly upregulated ER-phagy, MERCs-associated proteins, and pyroptosis-associated proteins in BRL-3A cells. DTT treatment for 36 h markedly increased CHOP protein expression and further increased MERCs-associated proteins and pyroptosis-associated protein levels, but moderately decreased ER-phagy levels. DDT treatment significantly downregulated CHOP enrichment in the promoter regions of ATG5, ATG12 and LC3 genes in BRL-3A cells. Pretreatment with bafilomycin A1 significantly increased DTT-induced BRL-3A cell death. CHOP overexpression in BRL-3A cells downregulated the levels of endoplasmic reticulophagy-related proteins, upregulated MERCs- and pyroptosis-related proteins, and reduced the colocalization of ER with autophagosomes and lysosomes, and increased its colocalization with the mitochondria, resulting in also elevated intramitochondrial Ca²⁺ levels and GSDMD-N protein and decreased mitochondrial transmembrane potential.</p><p><strong>Conclusions: </strong>CHOP promotes hepatocytes pyroptosis by suppressing ER-phagy and enhancing ER-mitochondrial interactions, which leads to increased Ca²⁺ shuttling from the ER to the mitochondria.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1648-1659"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Chronic MRSA periprosthetic joint infection induces formation of mature tertiary lymphoid structures and osteolysis in mice].","authors":"Maihemuti Munila, Lisi Huang, Jiakai Li, Rulong Cai, Qi Feng, Yudi Lin, Zehong Huang, Sijia Wu, Muhammad Umar, Hang Fang","doi":"10.12122/j.issn.1673-4254.2026.07.09","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.09","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the histomorphological and functional characteristics of tertiary lymphoid structures (TLS) in a mouse model of chronic knee periprosthetic joint infection (PJI) with methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and explore their association with local osteolysis.</p><p><strong>Methods: </strong>C57BL/6J mice receiving prosthesis implantation in the knee joint were randomized into control group and PJI group for injection of sterile PBS and of MRSA USA300 (1×10⁴ CFU) into the knee joint cavity, respectively. Peripheral blood leukocyte counts, erythrocyte sedimentation rates, and C-reactive protein levels were monitored regularly. Sixty days after the injections, periprosthetic tissues were harvested for HE staining to measure the cross-sectional area of mature TLS. Multiplex immunofluorescence staining was used to analyze spatial segregation of T and B cells (CD3/B220) and the network of follicular dendritic cells (FDCs) and detect Ki67 and PNAd expressions for assessing TLS functionality. The number of osteoclast per unit of bone surface (N.Oc/BS) in the periprosthetic region was determined using histomorphometric analysis with TRAP staining.</p><p><strong>Results: </strong>At 21 days post-surgery, the mice in PJI group showed significant elevation of all the peripheral blood inflammatory markers. Numerous mature TLS were observed in the periprosthetic tissues in PJI group with an average cross-sectional area significantly greater than that in the control group. The TLS in PJI group exhibited a typical spatial organization, characterized by a B-cell-centered core surrounded by T cells with an FDC network; abundant Ki67-positive proliferating cells were observed within the TLS, and PNAd-positive high endothelial venules were visible at the periphery and within the parenchyma. The N.Oc/BS was significantly greater in PJI group than in the control group (28.75±4.13 <i>vs</i> 1.63±0.74 mm^-1, <i>P</i><0.0001).</p><p><strong>Conclusions: </strong>Chronic MRSA infection induces formation of mature TLS with well-defined structural organization and functional activity in the periprosthetic region of mice, accompanied by significant bone loss.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1553-1562"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Medical prior-guided TabMap deep learning model for ovarian cancer prediction and interpretability analysis].","authors":"Jun Zhu, Shunqian Tan, Fangjun Huang, Guangyao Cai, Xin Zhen","doi":"10.12122/j.issn.1673-4254.2026.07.24","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.24","url":null,"abstract":"<p><strong>Objectives: </strong>To develop a TabMap image mapping and deep learning prediction framework that integrates medical prior knowledge to address the challenges of complex feature associations in tabular medical data and insufficient model interpretability in early ovarian cancer diagnosis.</p><p><strong>Methods: </strong>Based on clinical semantics, 36 medical diagnostic features were partitioned into 4 spatially continuous regions, namely the routine blood test partition, biochemical indicators partition, tumor markers partition, and other (coagulation, inflammation, and other clinical variables) partition. The Gromov-Wasserstein optimal transport algorithm was then employed to solve the optimal coupling between feature space and pixel space, thus generating TabMap images that preserve the topological structures. A lightweight convolutional neural network incorporating SE attention mechanism and global average pooling was designed to handle sample imbalance using class-weighted loss and weighted sampling strategies. Finally, class activation mapping (CAM) technique was utilized to visualize the model's decision-making process for interpretability analysis.</p><p><strong>Results: </strong>Experiments on a real ovarian cancer dataset demonstrated that the proposed method achieved a test accuracy of 91.82% with a precision of 89.96%, recall of 89.45%, F1-score of 0.8970 and balanced accuracy of 88.51%, representing accuracy improvements of 8.26%-14.93% over traditional machine learning methods and 2.28%-13.60% over standard deep learning models. Interpretability analysis showed that the model exhibits pronounced activation patterns in the tumor-marker region as well as in coagulation-, inflammation-, and age-related zones, consistent with established clinical understanding. The feature-importance ranking further underscored the pivotal roles of CA125, HE4, and other key biomarkers in guiding the model's decisions.</p><p><strong>Conclusions: </strong>The proposed medical prior-guided TabMap method effectively integrates domain knowledge with data-driven learning, which enhances both its prediction performance and clinical interpretability. This strategy provides a novel approach for deep learning modeling of tabular medical data with good clinical potentials.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1702-1710"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Phosphatidylinositol glycan anchor biosynthesis class T promotes malignant phenotype of colon cancer through the NF-κB signaling pathway].","authors":"Baichao Li, Bingzhe Wang, Xianghui Mei, Ye Tian, Liang Han, Jiuna Zhang","doi":"10.12122/j.issn.1673-4254.2026.09.16","DOIUrl":"10.12122/j.issn.1673-4254.2026.09.16","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the role of phosphatidylinositol glycan anchor biosynthesis class T (PIGT) in progression of colorectal cancer (CRC) and its molecular mechanism.</p><p><strong>Methods: </strong>The differential expression of PIGT in CRC and adjacent normal tissues was analyzed using GEPIA, UALCAN and TNMplot databases. Quantitative PCR and Western blotting were used to detect PIGT expression levels in CRC cell lines HCT116 and HCT8 and in normal colorectal mucosal NCM460 cells. The correlation between PIGT expression and 10-year recurrence-free survival of CRC patients was evaluated using Kaplan-Meier survival analysis. In CRC cells, the effects of PIGT knockdown on cell proliferation, clonogenic ability, migration, invasion and angiogenesis capacity were assessed. The NF-κB signaling pathway was identified as a key downstream pathway using transcriptome sequencing and pathway enrichment analysis, and the expressions of NF-κB key proteins and epithelial-mesenchymal transition (EMT) markers were detected using Western blotting. LPS rescue experiments were performed to confirm the regulatory relationship between PIGT and the NF-κB pathway. A nude mouse subcutaneous xenograft model was used to verify the oncogenic role of PIGT <i>in vivo</i>.</p><p><strong>Results: </strong>PIGT was significantly upregulated in CRC tissues and cell lines. High PIGT expression was closely associated with a shorter recurrence-free survival in patients with stage II to IV CRC. In CRC cells, PIGT knockdown significantly inhibited cell proliferation, migration, invasion and angiogenesis and effectively reversed EMT. Downregulation of PIGT reduced the phosphorylation level of P65 and suppressed NF-κB pathway activation, and these changes were effectively reversed by LPS treatment. In the tumor-bearing mice, PIGT knockdown markedly reduced subcutaneous tumor growth.</p><p><strong>Conclusions: </strong>PIGT promotes CRC progression and EMT by activating the NF‑κB signaling pathway, suggesting the potential of PIGT as both a prognostic biomarker and a therapeutic target for CRC.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 9","pages":"2173-2184"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[<i>Tongqiao Huoxue</i> Decoction-medicated rat cerebrospinal fluid attenuates oxygen and glucose deprivation-induced neuronal injury by suppressing fibrinogen-mediated NLRP3 inflammasome activation].","authors":"Yan Wang, Ping Huang, Yuqin Peng, Hao Sun, Ning Wang, Changzhong Wang","doi":"10.12122/j.issn.1673-4254.2026.07.08","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.08","url":null,"abstract":"<p><strong>Objectives: </strong>To examine the protective effects of cerebrospinal fluid from <i>Tongqiao Huoxue</i> Decoction-treated rats (TQHXD-CSF) against oxygen and glucose deprivation and reoxygenation (OGD/R)-induced injury in murine BV-2 microglial cells and co-cultured HT22 cells.</p><p><strong>Methods: </strong>In a Transwell co-culture system of BV-2 and HT22 cells, OGD/R+fibrinogen (FIB) injury was induced in BV-2 cells followed by treatment with TQHXD-CSF intervention, and HT22 cells in the lower chamber were cultured under normal conditions. The cells were observed for changes in cell morphology, viability, intracellular ROS level, apoptosis, M1/M2 polarization, and FIB-containing extracellular vesicles (EVs). The cellular expressions of NLRP3, ASC, caspase-1, GSDMD, IL-1β, and IL-18 were quantified using Western blotting, and FIB-NLRP3 binding was confirmed by pull-down assay.</p><p><strong>Results: </strong>OGD/R+FIB injury caused polarization of BV-2 cells to the pro-inflammatory M1 phenotype, increased CD86 expression and release of FIB-containing EVs, and activated the NLRP3 inflammasome. The co-cultured HT22 cells showed reduced cell viability, elevated ROS, and increased cells apoptosis. Treatment with TQHXD-CSF promoted M2 polarization in BV-2 cells, upregulated CD206 expression, suppressed FIB⁺ EVs secretion, and inhibited NLRP3 inflammasome activation in HT22 cells, which showed significantly lowered expressions of NLRP3, ASC, caspase-1, GSDMD, IL-1β, and IL-18 proteins and hence reduced inflammatory injury and cell apoptosis. Pull-down assay confirmed direct FIB and NLRP3 binding.</p><p><strong>Conclusions: </strong>In the co-culture system of BV-2 cells and HT22 cells, TQHXD-CSF treatment protects HT22 cells against OGD/R+FIB-induced injury by inhibiting FIB-containing EVs release from BV-2 cells and suppressing NLRP3 inflammasome activation.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1543-1552"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Re-transmission risk prediction of imported Chikungunya fever in Guangdong Province using the SEIR-SEI model].","authors":"Lishan Xu, Keke Li, Milai Yu, Qiuyi Zhi, Shengli An","doi":"10.12122/j.issn.1673-4254.2026.07.22","DOIUrl":"10.12122/j.issn.1673-4254.2026.07.22","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the effectiveness of different intervention measures against transmission of imported Chikungunya fever (CHIKF) in Guangdong Province to provide quantitative decision-making support for epidemic prevention and control.</p><p><strong>Methods: </strong>Based on the imported epidemic and mosquito vector monitoring data in Guangdong Province, a human-mosquito coupling SEIR-SEI transmission dynamics model was constructed. By adjusting the environmental carrying coefficient (<i>k</i>), the average number of bites (<i>θ</i>) and the interval between onset and diagnosis (1/ γ p), the local transmission risk and prevention and control threshold were simulated in scenarios of no intervention, single intervention (mosquito control/anti-mosquito/case isolation) and comprehensive intervention.</p><p><strong>Results: </strong>A total of 65 imported cases were reported in Guangdong Province from 2008 to 2019. The simulation results showed limited prevention and control effect of a single intervention measure, and reducing <i>k</i> to below 0.001 or <i>θ</i> to below 0.1 was essential to block re-transmission of the epidemic; shortening 1/ γ p could significantly reduce the incidence peak and shorten the epidemic cycle. The effect of comprehensive intervention was significantly better than that of single intervention. The critical threshold of blocking transimission varied with the combination of parameters. For a <i>k</i> value no greater than 0.5, <i>θ</i> needed to be controlled below 0.4 and 1/ γ p below 3 days, or a more stringent alternative combination should be adopted.</p><p><strong>Conclusions: </strong>Comprehensive intervention measures should be implemented to contain imported CHIKF epidemics in Guangdong Province. It is recommended that <i>k</i> be controlled below 0.5, <i>θ</i> below 0.4, and 1/ γ p within 3 days, and these thresholds can provide reference for prevention and control of similar epidemics.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 7","pages":"1696-1702"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13391602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148562617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Quantitative susceptibility reconstruction guided by a multi-scale T1-weighted image attention mechanism].","authors":"Piaoran Li, Quan Tao, Fusheng Oyang, Baoliang Guo, Qiugen Hu, Yanqiu Feng","doi":"10.12122/j.issn.1673-4254.2026.09.23","DOIUrl":"10.12122/j.issn.1673-4254.2026.09.23","url":null,"abstract":"<p><strong>Objectives: </strong>To improve the accuracy and structural consistency of quantitative susceptibility mapping (QSM) reconstruction through a deep learning network guided by multi-scale T1-weighted image attention.</p><p><strong>Methods: </strong>A multi-scale T1-weighted attention-driven deep QSM network (T1w-ADQSM) was proposed to enhance the precision and structural fidelity of single-orientation QSM reconstruction. The method incorporates structural features from T1-weighted images and employs an attention module to guide the network to focus on the anatomical boundaries and key regions. Experimental comparisons were conducted among T1w-ADQSM,truncated k-space division (TKD) method, morphology enabled dipole inversion (MEDI) and QSMnet. Quantitative evaluations were performed using the metrics including high-frequency error norm (HFEN), structural similarity index measure (SSIM),normalized root mean square error (NRMSE), and peak signal-to-noise ratio (PSNR).</p><p><strong>Results: </strong>In healthy volunteers, T1w-ADQSM achieved the highest PSNR (43.12±1.19) and the lowest NRMSE (51.98±3.65) compared with TKD, MEDI, and QSMnet. T1w-ADQSM outperformed QSMnet across all the 4 quantitative metrics with statistically significant differences (<i>P</i><0.05). Further validation based on the COSMOS reference reconstructed from multi-orientation data showed that T1w-ADQSM exhibited better structural consistency than QSMnet, and the reconstruction results were closer to the COSMOS reference.</p><p><strong>Conclusions: </strong>The proposed multi-scale T1-weighted image attention-driven deep learning reconstruction method improves the accuracy and structural consistency of QSM.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 9","pages":"2258-2265"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Anwulignan inhibits malignant phenotypes of gastric cancer cells by antagonizing activation of the JAK1/STAT3 signaling pathway].","authors":"Xinyue Liu, Keni Zhang, Tong Qiao, Lin Yin, Longtao Zhang, Xinke Cheng, Ben Wang, Jinlin Cao, Zhijun Geng, Hao Zhao","doi":"10.12122/j.issn.1673-4254.2026.09.22","DOIUrl":"10.12122/j.issn.1673-4254.2026.09.22","url":null,"abstract":"<p><strong>Methods: </strong>To evaluate the inhibitory effect of anwulignan (ANW) on malignant biological behaviors of gastric cancer cells and explore its underlying molecular mechanism.</p><p><strong>Methods: </strong>The inhibitory effect of ANW on viability and proliferation of gastric cancer HGC-27 and SGC-7901 cells were evaluated using CCK-8 assay, EdU staining and colony formation assay. In a nude mouse model bearing gastric cancer xenografts, the effect of ANW on tumor cell proliferation was examined using immunohistochemistry for Ki-67. The changes in cell cycle, apoptosis, migration, invasion, and expressions of MMPs and epithelial-mesenchymal transition (EMT) markers following ANW treatment were detected using flow cytometry, TUNEL staining, Western blotting, Transwell assay, and qPCR. The role of the JAK1/STAT3 signaling pathway in mediating the anti-tumor activity of ANW was validated by detecting JAK1/STAT3 phosphorylation, pathway activation and siRNA-mediated JAK1 knockdown experiments.</p><p><strong>Results: </strong>In HGC-27 and SGC-7901 cells, ANW dose-dependently suppressed cell proliferation and colony formation, induced G1 cell cycle arrest, and downregulated the expressions of cyclin D1/CDK2 and P53. ANW treatment significantly inhibited tumor growth and decreased Ki-67 positivity in the mouse xenografts. ANW effectively promoted cell apoptosis, suppressed Bcl-2 expression, upregulated cleaved caspase-3 and Bax expressions, inhibited migration and invasion, lowered MMP-2/MMP-9 expressions, and blocked EMT progression in gastric cancer cells. Mechanistically, ANW significantly inhibited JAK1/STAT3 phosphorylation, while the JAK1/STAT3 activator RO8191 reversed the anti-tumor effect of ANW; JAK1 knockdown mimicked and enhanced the inhibitory effect of ANW on gastric cancer cells. ANW significantly inhibited EMT in the mouse xenografts, and this effect was attenuated by RO8191 treatment.</p><p><strong>Conclusions: </strong>ANW inhibits malignant phenotype of gastric cancer cells by inhibiting the activation of the JAK1/STAT3 signaling pathway, thereby inducing cell cycle arrest, promoting apoptosis, and suppressing EMT and MMP-mediated invasion and migration, suggesting the potential of ANW as a therapeutic agent for gastric cancer.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 9","pages":"2242-2257"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Development of a machine learning-based model for assessing pulmonary function in elderly patients].","authors":"Cuiyan Chen, Wenxian Zhang, Yilin Cai, Mei Ouyang, Ye Liang, Mingbiao Liang, Huiying Liang","doi":"10.12122/j.issn.1673-4254.2026.09.09","DOIUrl":"10.12122/j.issn.1673-4254.2026.09.09","url":null,"abstract":"<p><strong>Objectives: </strong>To develop a machine learning-based model with good generalization ability for pulmonary function assessment in elderly patients with impaired pulmonary function.</p><p><strong>Methods: </strong>A total of 7720 elderly patients undergoing pulmonary function testing (PFT) in Guangdong Provincial People's Hospital between September 1, 2020 and December 31, 2024 were enrolled. After variable screening by univariate analysis and LASSO regression, the patients were randomly assigned in an 8:2 ratio to the training set (<i>n</i>=6176) and test set (<i>n</i>=1544). Five regression models were constructed for predicting forced vital capacity (FVC) and forced expiratory volume in one second (FEV₁), and their performance was evaluated based on Pearson correlation coefficient (<i>r</i>) and intraclass correlation coefficient (ICC). Feature contributions were interpreted using Shapley additive explanations, and the classification performance of the models for high-risk populations was assessed.</p><p><strong>Results: </strong>XGBoost showed the best predictive performance in the elderly patients (<i>r</i>=0.733 for FVC and 0.711 for FEV₁; ICC=0.694 for FVCand 0.668 for FEV₁). Male gender and a younger age showed positive effects on pulmonary function, while female gender and an advanced age showed negative effects. The optimal cut-off values of FVC% and FEV₁% were 82.93% and 85.46%, with AUC of 0.700 and 0.706, respectively.</p><p><strong>Conclusions: </strong>The XGBoost-based model developed in this study is simple and reliable with good generalization ability for pulmonary function assessment in elderly patients.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"46 9","pages":"2091-2100"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13624982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149081707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}