Nan fang yi ke da xue xue bao = Journal of Southern Medical University最新文献

{"title":"[Overwork induces vascular endothelial barrier dysfunction in mice].","authors":"Y Liao, X Ma, S Deng, S Chen, Y Li","doi":"10.12122/j.issn.1673-4254.2024.09.22","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2024.09.22","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the impact of overwork on vascular endothelial barrier function in mice.</p><p><strong>Methods: </strong>Thirty KM mice were randomized equally into control, overwork for 2 weeks (W2) group and 4 weeks (W4) group. In the latter two groups, the mice were subjected to continuous standing in water for 8 h followed by restraint for 3 h to simulate overwork on a daily basis for 2 and 4 weeks. After modeling, 4 mice from each group were intraperitoneally injected with Evans blue dye to assess vascular permeability. In the other 6 mice, serum IL-1β levels were measured using ELISA, and arterial tissues were collected for histological examination and detection of mRNA expressions of <i>occludin, claudin-5, ZO-1</i>, <i>JAM-A</i> and <i>VE-cadherin</i>; immunofluorescence assay was used to detect the protein expressions of claudin-5, ZO-1, VE-cadherin, and Syndecan-1.</p><p><strong>Results: </strong>The mice in W2 and W4 groups exhibited slower weight gain, hair loss, reduced activity, and significantly increased serum IL-1β levels. Vascular permeability was significantly increased in W4 group. In W2 group, the endothelial cells were swollen and dissociated, and the intima was rough and irregular; arterial intimal rupture was observed in W4 group. The mRNA expressions of <i>occludin, claudin-5, ZO-1 and JAM-A</i> in the arterial tissues were significantly increased in W2 group but decreased in W4 group, while <i>VE-cadherin</i> mRNA expression were reduced in both groups (<i>P</i> < 0.05). The protein expressions of claudin-5, ZO-1, VE-cadherin, and Syndecan-1 were all significantly reduced in W4 group.</p><p><strong>Conclusion: </strong>Prolonged overwork can cause damage of the intercellular junction complexes in arterial endothelial cells and the endothelial glycocalyx to result in impaired barrier function and increased vascular permeability in mice.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Curcumin alleviates septic lung injury in mice by inhibiting TXNIP/TRX-1/GPX4-mediated ferroptosis].","authors":"K Chen, Z Meng, J Min, J Wang, Z Li, Q Gao, J Hu","doi":"10.12122/j.issn.1673-4254.2024.09.21","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.21","url":null,"abstract":"<p><strong>Objective: </strong>To investigate whether curcumin alleviates septic lung injury by inhibiting ferroptosis through modulating the TXNIP/TRX-1/GPX4 pathway.</p><p><strong>Methods: </strong>Male C57BL/6 mice were randomly divided into Sham group, cecal ligation puncture (CLP)-induced sepsis group, CLP with curcumin treatment (50, 100, and 200 mg/kg) groups, and CLP with both curcumin (200 mg/kg) and TRX-1 inhibitor PX-12 (25 mg/kg) treatment group. Inflammatory factors, MDA, MPO, and GSH levels in the lung tissue of the mice were detected. Beas-2B cells stimulated with lipopolysaccharide (LPS; 1 μg/mL) were treated with 2.5, 5, or 10 μmol/L curcumin or with 10 μmol/L curcumin combined with 5 μmol/L PX-12, and the changes in MDA, Fe²⁺ and ROS levels were assessed. Western blotting was performed to detect the protein expressions of TXNIP, TRX-1, GPX4 and X-CT in both the mouse lung tissues and Beas-2B cells.</p><p><strong>Results: </strong>The mice with CLP-induced sepsis showed severe lung injury with elevated expressions of IL-6, IL-1β, TNF-α, MDA and MPO and decreased GSH expression. In Beas-2B cells, LPS stimulation significantly increased MDA and Fe²⁺ levels and ROS release, increased TXNIP protein expression, and lowered the protein expression levels of TRX-1, GPX4 and X-CT, and these changes were also observed in the septic mice. Curcumin treatments at different concentrations obviously alleviated lung injury in the septic mice and reduced LPS-induced injury in Beas-2B cells. Curcumin significantly decreased the release of inflammatory factors, MDA and MPO, increased GSH level, lowered Fe²⁺, MDA and ROS levels, increased TXNIP protein expression, and lowered the protein expressions of TRX-1, GPX4 and X-CT in both septic mouse lung tissues and LPS-stimulated Beas-2B cells. The protective effect of curcumin was effectively blocked by PX-12 treatment.</p><p><strong>Conclusion: </strong>Curcumin inhibits ferroptosis and alleviates septic lung injury in mice by elevating TRX-1 and GPX4 and decreasing TXNIP in the lung tissue.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[<i>Pterocarya hupehensis</i> Skan total flavones ameliorate rheumatoid arthritis in rats by suppressing formation of neutrophil extracellular traps].","authors":"R Yang, Y Shu, H Wen, X Cai, Z Wang, C Zhang, Y Xiang, H Wu","doi":"10.12122/j.issn.1673-4254.2024.09.03","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2024.09.03","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the therapeutic mechanism of <i>Pterocarya hupehensis</i> Skan total flavonoids (PHSTF) for rheumatoid arthritis (RA).</p><p><strong>Methods: </strong>Twenty-five male SD rats were randomly divided into normal control group, RA model group, PHSTF treatment (45 and 90 mg/kg) groups, and Tripterygium glycosides (TPG) tablet (10 mg/kg) group (<i>n</i>=5). Except for those in the normal control group, all the rats were subjected to collagen-induced arthritis (CIA) modeling using a secondary immunization method, after which PHSTF and TPG were administered via gavage once daily for 4 weeks. After the treatments, serum levels of TNF-<i>α</i> and IL-1β were measured using ELISA, and ankle joint pathologies were assessed with HE staining; the expression of citrullinated histone H3 (Cit-H3), a neutrophil extracellular trap (NET) marker, in the ankle joints was evaluated with immunohistochemistry. In primary cultures of rat peripheral blood neutrophils stimulated with phorbol ester (PMA), the effects of PHSTF (100 and 200 μg/mL) on the expressions of Cit-H3, peptidylarginine deiminase 4 (PADI4), neutrophil elastase (NE), and myeloperoxidase (MPO) were examined with Western blotting; immunofluorescence assay was used to observe Cit-H3 expression and NET formation in the cells.</p><p><strong>Results: </strong>In the CIA rat models, PHSTF significantly alleviated ankle swelling, decreased serum levels of TNF-<i>α</i> and IL-1β, improved histopathological changes in the ankle joints, and reduced Cit-H3 expression in both the serum and ankle joint cartilage. In the isolated rat neutrophils, PHSTF showed no significant effect on cell viability but strongly inhibited PMA-induced NET release.</p><p><strong>Conclusion: </strong>PHSTF can alleviate RA by inhibiting the formation of NETs.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Respiratory complications of propofol, sevoflurane, and dexmedetomidine anesthesia for fiberoptic bronchoscopy in children aged 1 month to 3 years: a randomized trial].","authors":"Shafa Amir, Montasery Mohammad, Shahhosseini Sedighe, Keivanfar Majid, Maghami Mehr Asieh, Ebrahim Babaei Mahtab, Jafari Mohammad","doi":"10.12122/j.issn.1673-4254.2024.09.01","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2024.09.01","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effect of propofol, sevoflurane, and dexmedetomidine on respiratory complications in children undergoing fiberoptic bronchoscopy (FOB).</p><p><strong>Methods: </strong>This double-blind randomized clinical trial was conducted among 120 children aged 1 month to 3 years undergoing FOB. The patients were randomized into 3 groups (<i>n</i>=40) for anesthesia induction with sevoflurane inhalation, 1 mg/kg propofol, or 1 μg/kg dexmedetomidine before bronchoscopy, and the changes in hemodynamic parameters, sedation level, and respiratory complications during and after the procedure were assessed.</p><p><strong>Results: </strong>The patients' heart rate during bronchoscopy was significantly lower and the mean arterial blood pressure significantly higher in dexmedetomidine group than in sevoflurane and propofol groups (<i>P</i> < 0.05). Cough during bronchoscopy did not occur in any of the cases in propofol group, while the highest frequency of cough was recorded in dexmedetomidine group. The incidence of laryngospasm in the propofol group (12.5%) was significantly lower than those in sevoflurane and dexmedetomidine groups (30% and 32.5%, respectively) (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>Sevoflurane and propofol are safe and suitable for anesthesia induction in children below 3 years of age undergoing diagnostic FOB and can achieve better sedative effect and lower the incidences of cough and respiratory complications as compared with dexmedetomidine.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A lung sound classification model with a spatial and channel reconstruction convolutional module].","authors":"N Ye, C Wu, J Jiang","doi":"10.12122/j.issn.1673-4254.2024.09.12","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2024.09.12","url":null,"abstract":"<p><strong>Objective: </strong>To construct a model with a spatial and channel reconstruction convolutional module for accurate identification and classification of lung sound data.</p><p><strong>Methods: </strong>We propose a convolutional network architecture combining the spatial-channel reconstruction convolution (SCConv) module. A lung sound feature extraction method combining the dual tunable Q-factor wavelet transform (DTQWT) with the triple Wigner-Ville transform (WVT) was used to improve the model's ability to capture the key features of the lung sounds by adaptively focusing on the important channel and spatial features. The performance of the model for classification of normal, crackles, wheezes, and crackles with wheezes was tested using the ICBHI2017 dataset.</p><p><strong>Results and conclusion: </strong>The accuracy, sensitivity, specificity and F1 score of the proposed method reached 85.68%, 93.55%, 86.79% and 90.51%, respectively, demonstrating its good performance in classification tasks in the ICBHI2017 lung sound database, especially for distinguishing normal from abnormal lung sounds.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A multi-constraint optimal puncture path planning algorithm for percutaneous interventional radiofrequency thermal fusion of the L5/S1 segments].","authors":"H Liu, Z Su, C Huang, L Zhao, Y Chen, Y Zhou, H Lü, Q Feng","doi":"10.12122/j.issn.1673-4254.2024.09.19","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2024.09.19","url":null,"abstract":"<p><strong>Objective: </strong>To minimize variations in treatment outcomes of L5/S1 percutaneous intervertebral radiofrequency thermocoagulation (PIRFT) arising from physician proficiency and achieve precise quantitative risk assessment of the puncture paths.</p><p><strong>Methods: </strong>We used a self-developed deep neural network DWT-UNet for automatic segmentation of the magnetic resonance (MR) images of the L5/S1 segments into 7 key structures: L5, S1, Ilium, Disc, N5, Dura mater, and Skin, based on which a needle insertion path planning environment was modeled. Six hard constraints and 6 soft constraints were proposed based on clinical criteria for needle insertion, and the physician's experience was quantified into weights using the analytic hierarchy process and incorporated into the risk function for needle insertion paths to enhance individual case adaptability. By leveraging the proposed skin entry point sampling sub-algorithm and Kambin's triangle projection area sub-algorithm in conjunction with the analytic hierarchy process, and employing various technologies such as ray tracing, CPU multi-threading, and GPU parallel computing, a puncture path was calculated that not only met clinical hard constraints but also optimized the overall soft constraints.</p><p><strong>Results: </strong>A surgical team conducted a subjective evaluation of the 21 needle puncture paths planned by the algorithm, and all the paths met the clinical requirements, with 95.24% of them rated excellent or good. Compared with the physician's planning results, the plans generated by the algorithm showed inferior D_Ilium, D_S1, and Depth (<i>P</i> < 0.05) but much better D_Dura, D_L5, D_N5, and A_Kambin (<i>P</i> < 0.05). In the 21 cases, the planning time of the algorithm averaged 7.97±3.73 s, much shorter than that by the physicians (typically beyond 10 min).</p><p><strong>Conclusion: </strong>The multi-constraint optimal puncture path planning algorithm offers an efficient automated solution for PIRFT of the L5/S1 segments with great potentials for clinical application.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Gastrodin improves microglia-mediated inflammatory response after hypoxic-ischemic brain damage in neonatal rats <i>via</i> PI3K/AKT pathway].","authors":"H Zuo, Z Duan, Z Wang, T Guo, J Shi, H Shi, J Li","doi":"10.12122/j.issn.1673-4254.2024.09.11","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.11","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanism of gastrodin for inhibiting microglia-mediated inflammation after hypoxicischemic brain damage (HIBD) in neonatal rats.</p><p><strong>Methods: </strong>Thirty-nine 3-day-old SD rats were randomly divided into sham group, HIBD group and gastrodin treatment group. Western blotting was used to detect the expressions of TNF-α, IL-1β, IL-10 and TGF- β1 in the corpus callosum of the rats. The potential targets of gastrodin for treatment of HIBD were screened by network pharmacology analysis. The expressions of PI3K/AKT signaling pathway proteins following HIBD-induced microglial activation in the rats and in cultured microglial BV-2 cells with oxygen-glucose deprivation (OGD) were detected with Western blotting. The effects of LY294002 (a specific inhibitor of the PI3K/AKT pathway) and gastrodin on TNF-α and TGF-β1 mRNA levels in BV-2 cells with OGD was detected with RT-qPCR.</p><p><strong>Results: </strong>In the neonatal rats with HIBD, gastrodin treatment significantly decreased TNF-α and IL-1β expressions and enhanced IL-10 and TGF-β1 expressions in the ischemic corpus callosum. Network pharmacology analysis showed significant enrichment of the PI3K/AKT signaling pathway and a strong binding between gastrodin and PI3K. Gastrodin significantly promoted PI3K and AKT phosphorylation in neonatal rats with HIBD and in BV-2 cells exposed to OGD. In BV-2 cells with OGD, gastrodin obviously suppressed OGD-induced increase of TNF-α and reduction of TGF-β1 mRNA expressions, and this effect was strongly attenuated by LY294002 treatment.</p><p><strong>Conclusion: </strong>Gastrodin can inhibit microglia-mediated inflammation in neonatal rats with HIBD by regulating the PI3K/AKT signaling pathway.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Bardoxolone methyl alleviates acute liver injury in mice by inhibiting NLRP3 inflammasome activation].","authors":"M Li, W Zhang, M Hua","doi":"10.12122/j.issn.1673-4254.2024.09.05","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.05","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the inhibitory effect of bardoxolone methyl (CDDO-Me) on activation of NLRP3 inflammasome and its mechanism for alleviating acute liver injury (ALI).</p><p><strong>Methods: </strong>Mouse bone marrow-derived macrophages (BMDM) and THP-1 cells were pre-treated with CDDO-Me followed by treatment with Nigericin, ATP, MSU, intracellular LPS transfection for activation of NLRP3 inflammasomes, or poly A: T for activation of AIM2 inflammasomes. The levels of caspase-1 and IL-1β in the cell culture supernatant was determined with Western blotting and ELISA to assess the inhibitory effect of CDDO-Me on NLRP3 inflammasomes and its specificity. In the animal experiment, male C57BL/6J mouse models of acetaminophen-induced ALI were treated with low-dose (20 mg/kg) and high-dose (40 mg/kg) CDDO-Me, and the changes in serum levels of IL-1β, TNF- <i>α</i>, AST and ALT were measured by ELISA and liver tissue pathology was observed using HE staining.</p><p><strong>Results: </strong>In mouse BMDM and THP-1 cells, CDDO-Me dose-dependently inhibited the activation of NLRP3 inflammasomes without significantly affecting the secretion of non-inflammasome-related inflammatory factors IL-6 and TNF-<i>α</i> or AIM2 inflammasome activation. In the mouse models of ALI, CDDO-Me treatment at both the low and high doses significantly reduced serum levels of IL-1β, AST and ALT, ameliorated histological changes and reduced inflammatory cell infiltration in the liver tissue, and the effects exhibited a distinct dose dependence.</p><p><strong>Conclusion: </strong>CDDO-Me can specifically inhibit the activation of NLRP3 inflammasomes to alleviate acetaminophen-induced ALI in mice.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[High RNF7 expression enhances PD-1 resistance of non-small cell lung cancer cells by promoting CXCL1 expression and myeloid-derived suppressor cell recruitment <i>via</i> activating NF-κB signaling].","authors":"N Zhong, H Wang, W Zhao, Z Sun, B Geng","doi":"10.12122/j.issn.1673-4254.2024.09.10","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.10","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanism of RNF7 for regulating myeloid-derived suppressor cells (MDSCs) in nonsmall cell lung cancer (NSCLC).</p><p><strong>Methods: </strong>TIMER2.0 database and immunohistochemistry were used to analyze RNF7 expression level and its correlation with immune cell infiltration in non-small cell lung cancer. The impact of RNF7 expression levels on prognosis of lung cancer patients was analyzed using Kaplan-Meier survival analysis. CMT-167 cells with RNF7 overexpression or knockdown were inoculated subcutaneously in C57BL/6 mice, and the mice in RNF7 knockdown group were treated with anti-PD-1 or IgG isotype control 7 days after the inoculation. The tumor tissues were harvested after 30 days for tumor volume measurement, detection of S100A8+A9 and Gr-1 expressions with immunohistochemistry, and analysis of MDSC infiltration. Gene set enrichment analysis (GSEA) was performed to identify the potential pathways regulated by RNF7 in NSCLC. Western blotting and luciferase assays were used to assess the impact of RNF7 on the NF-κB signaling pathway. ELISA and RT-qPCR were used to measure chemokine (C-X-C motif) ligand 1 (CXCL1) expression.</p><p><strong>Results: </strong>RNF7 expression was significantly upregulated in NSCLC, and high RNF7 expression levels were associated with poor prognosis of the patients (<i>P</i> < 0.001). TIMER2.0 analysis revealed a positive correlation between RNF7 expression and MDSC infiltration (<i>P</i> < 0.001). GSEA suggested that RNF7 was enriched in the NF-κB signaling pathway. In NSCLC cells, RNF7 knockdown significantly inhibited NF-κB activation and reduced CXCL1 expression. In the tumor-bearing mice, RNF7 overexpression significantly increased MDSC infiltration in the tumor tissue, and RNF7 knockdown obviously reduced MDSC infiltration and enhanced the efficacy of anti-PD-1 therapy.</p><p><strong>Conclusion: </strong>High expression of RNF7 in NSCLC cells promotes CXCL1 expression by activating the NF-κB signaling pathway, thus leading to the chemotactic recruitment of MDSCs, which contributes to tumor resistance to antiPD-1 therapy.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Role of Abelson interactor 2 in progression and prognosis of gastric cancer and its regulatory mechanisms].","authors":"X Chen, H Lu, Z Wang, L Wang, Y Xia, Z Geng, X Zhang, X Song, Y Wang, J Li, J Hu, L Zuo","doi":"10.12122/j.issn.1673-4254.2024.09.04","DOIUrl":"10.12122/j.issn.1673-4254.2024.09.04","url":null,"abstract":"<p><strong>Objective: </strong>To explore the regulatory role of Abelson interactor 2 (ABI2) in progression and prognosis of gastric cancer.</p><p><strong>Methods: </strong>TIMER2.0, GEPIA, Kaplan-Meier Plotter and DAVID databases were used to analyze ABI2 expression in pancancer and its association with the prognosis of gastric cancer. Gastric cancer and adjacent tissues from 120 patients undergoing radical gastrectomy in our hospital between January, 2016 and October, 2018 were examined for ABI2 expression and its correlation with disease progression and prognosis. MGC-803 cell models of ABI2 knockdown and overexpression were established for observing the changes in cell proliferation, migration, and invasion, and the impact of ABI2 expression modulation on xenograft growth was evaluated in nude mice.</p><p><strong>Results: </strong>Database analysis and examination of the clinical samples showed that ABI2 was highly expressed in gastric cancer tissues. Survival analysis suggested that gastric cancer patients with a high expression of ABI2 had a reduced postoperative 5-year survival rate (<i>P</i> < 0.0001), and further Cox univariate and multivariate survival analyses indicated that a high ABI2 expression was an independent risk factor affecting the patients survival outcomes (<i>P</i>=0.022, <i>HR</i>=1.887, 95% <i>CI</i>: 1.096-3.249). Enrichment analysis suggested the involvement of ABI2 in Wnt signaling. In MGC-803 cells, ABI2 overexpression promoted cell proliferation and xenograft growth in nude mice, increased the expressions of vimentin and N-cadherin, and lowered E-cadherin expression, while ABI2 knockdown produced the opposite effects. Mechanistic analysis revealed that ABI2 overexpression promoted the expressions of Wnt2 and β-catenin in both MGC-803 cells and the xenografts, and their expressions were significantly lowered by ABI2 knockdown.</p><p><strong>Conclusion: </strong>ABI2 is highly expressed in gastric cancer, which affects long-term prognosis of the patients, possible due to its regulatory effect on Wnt signaling to promote proliferation, migration and invasion of gastric cancer cells.</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}