南方医科大学学报杂志最新文献

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[Electroacupuncture combined with rehabilitation training improves neurological function of mice with cerebral ischemia by promoting astrocyte transdifferentiation]. [电针联合康复训练通过促进星形胶质细胞转分化改善脑缺血小鼠神经功能]。
南方医科大学学报杂志 Pub Date : 2025-07-20 DOI: 10.12122/j.issn.1673-4254.2025.07.09
Dongning Tang, Yunyun Kang, Wenjie He, Qing Xia
{"title":"[Electroacupuncture combined with rehabilitation training improves neurological function of mice with cerebral ischemia by promoting astrocyte transdifferentiation].","authors":"Dongning Tang, Yunyun Kang, Wenjie He, Qing Xia","doi":"10.12122/j.issn.1673-4254.2025.07.09","DOIUrl":"10.12122/j.issn.1673-4254.2025.07.09","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the effects of acupuncture combined with rehabilitation training for promoting transdifferentiation of astrocytes into neurons in mice after cerebral ischemia.</p><p><strong>Methods: </strong>Male C57/BL6J mice were subjected to intracerebral microinjection of an adeno-associated virus carrying the GFAP promoter for NeuroD1 and Ngn2 overexpression in the astrocytes, followed 3 or 12 days later by electrocoagulation of the distal middle cerebral artery. After modeling, the mice were randomly divided into model group without interventions and intervention group treated with electroacupuncture at the acupoints Baihui (GV20), left Hegu (LI4), Neiguan (PC6), Zusanli (ST36), and Yanglingquan (GB34) 24 h after surgery. The mice in the intervention group were housed individually in cages with running wheels, and their activity was recorded every 24 h. Neurological function scores of the mice were assessed on the 1st, 14th, and 21st days after modeling. Transdifferentiation of astrocytes in the target brain regions was observed using double immunofluorescence staining.</p><p><strong>Results: </strong>Compared with those in the model group, the mice receiving eletroacupuncture and rehabilitation training showed significant improvement of neurological deficits at 14 and 21 days after modeling. The GFAP promoter of the AAV2/5 vector specifically labeled the local astrocytes, and compared with that that in the model group, the number of AAV-positive cells colabeled with the neuronal marker DCX significantly increased after 14 days of electroacupuncture and rehabilitation intervention, and the number of AAV-positive cells colabeled with the neuronal marker NeuN significantly increased after 21 days of intervention.</p><p><strong>Conclusions: </strong>In mice with cerebral ischemia, electroacupuncture and rehabilitation training can promote transdifferentiation of astrocytes into neurons in the ischemic brain region, and the efficiency of transdifferentiation is positively correlated with the improvement of motor function.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 7","pages":"1434-1441"},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[High PRELID1 expression promotes epithelial-mesenchymal transition in gastric cancer cells and is associated with poor prognosis]. [PRELID1高表达促进胃癌细胞上皮-间质转化,与预后不良相关]。
南方医科大学学报杂志 Pub Date : 2025-07-20 DOI: 10.12122/j.issn.1673-4254.2025.07.21
Xuan Wu, Jiamin Fang, Weiwei Han, Lin Chen, Jing Sun, Qili Jin
{"title":"[High PRELID1 expression promotes epithelial-mesenchymal transition in gastric cancer cells and is associated with poor prognosis].","authors":"Xuan Wu, Jiamin Fang, Weiwei Han, Lin Chen, Jing Sun, Qili Jin","doi":"10.12122/j.issn.1673-4254.2025.07.21","DOIUrl":"10.12122/j.issn.1673-4254.2025.07.21","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the correlation of PRELID1 with gastric cancer (GC) progression, prognosis, and epithelial-mesenchymal transition (EMT) and the underlying mechanisms.</p><p><strong>Methods: </strong>We analyzed the data of 115 patients undergoing radical gastrectomy for GC in our hospital between February, 2018 and March, 2023 to explore the correlation of PRELID1 expression level in GC tissues with tumor progression and patient prognosis. In cultured GC cells, the effects of lentivirus-mediated overexpression or interference of PRELID1 were observed on cell migration, invasion and EMT.</p><p><strong>Results: </strong>Immunohistochemical staining revealed significantly higher PRELID1 expression in GC tissues (<i>P</i><0.001), whose expression level was positively correlated with CEA ≥5 ng/mL (<i>P</i>=0.007), CA199 ≥37 U/mL (<i>P</i>=0.007), G<sub>3-4</sub> stages (<i>P</i>=0.001), T<sub>3-4</sub> stages (<i>P</i>=0.001), and N<sub>2-3</sub> stages (<i>P</i>=0.020). Univariate and Cox multifactorial analysis showed that high PRELID1 level was an independent risk factor affecting 5-year survival of GC patients (<i>P</i>=0.001). In cultured GC cells, PRELID1 overexpression obviously promoted cell proliferation, migration, invasion, and the expressions of MMP2 and MMP9, and interference of PRELID1 produced the opposite changes. PRELID1 overexpression also increased the expressions of N-cadherin and vimentin and reduced the expression of E-cadherin. Mechanistic analyses showed that up-regulation of PRELID1 increased the expression of p-PI3K, p-AKT, and p-mTOR in GC cells, whereas its interference caused the opposite changes; the application of 740 Y-P, a PI3K/AKT pathway activator, significantly enhanced the migration, invasion, and EMT of GC cells with PRELID1 knockdown.</p><p><strong>Conclusions: </strong>PRELID1 is highly expressed in GC and affects prognosis of the patients, and its high expression promotes migration, invasion and epithelial mesenchymal transition of GC cells possibly by activating the PI3K/AKT/mTOR pathway.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 7","pages":"1535-1542"},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Live combined Bacillus subtilis and Enterococcus faecium improves glucose and lipid metabolism in type 2 diabetic mice with circadian rhythm disruption via the SCFAs/GPR43/GLP-1 pathway]. [枯草芽孢杆菌和屎肠球菌联合活菌通过SCFAs/GPR43/GLP-1途径改善昼夜节律紊乱的2型糖尿病小鼠的糖脂代谢]。
南方医科大学学报杂志 Pub Date : 2025-07-20 DOI: 10.12122/j.issn.1673-4254.2025.07.15
Ruimin Han, Manke Zhao, Junfang Yuan, Zhenhong Shi, Zhen Wang, Defeng Wang
{"title":"[Live combined <i>Bacillus subtilis</i> and <i>Enterococcus faecium</i> improves glucose and lipid metabolism in type 2 diabetic mice with circadian rhythm disruption via the SCFAs/GPR43/GLP-1 pathway].","authors":"Ruimin Han, Manke Zhao, Junfang Yuan, Zhenhong Shi, Zhen Wang, Defeng Wang","doi":"10.12122/j.issn.1673-4254.2025.07.15","DOIUrl":"10.12122/j.issn.1673-4254.2025.07.15","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the effects of live combined <i>Bacillus subtilis</i> and <i>Enterococcus faecium</i> (LCBE) on glucose and lipid metabolism in mice with type 2 diabetes mellitus (T2DM) and circadian rhythm disorder (CRD) and explore the possible mechanisms.</p><p><strong>Methods: </strong>KM mice were randomized into normal diet (ND) group (<i>n</i>=8), high-fat diet (HFD) group (<i>n</i>=8), and rhythm-intervention with HFD group (<i>n</i>=16). After 8 weeks of feeding, the mice were given an intraperitoneal injection of streptozotocin (100 mg/kg) to induce T2DM. The mice in CRD-T2DM group were further randomized into two equal groups for treatment with LCBE (225 mg/kg) or saline by gavage; the mice in ND and HFD groups also received saline gavage for 8 weeks. Blood glucose level of the mice was measured using a glucometer, and serum levels of Bmal1, PER2, insulin, C-peptide and lipids were determined with ELISA. Colon morphology and hepatic lipid metabolism of the mice were examined using HE staining and Oil Red O staining, respectively, and fecal short-chain fatty acids (SCFAs) was detected using LC-MS; GPR43 and GLP-1 expression levels were analyzed using RT-qPCR and Western blotting.</p><p><strong>Results: </strong>Compared with those in CRD-T2DM group, the LCBE-treated mice exhibited significant body weight loss, lowered levels of PER2, insulin, C-peptide, total cholesterol (TC) and LDL-C, and increased levels of Bmal1 and HDL-C levels. LCBE treatment significantly increased SCFAs, upregulated GPR43 and GLP-1 expressions at both the mRNA and protein levels, and improved hepatic steatosis and colon histology.</p><p><strong>Conclusions: </strong>LCBE ameliorates lipid metabolism disorder in CRD-T2DM mice by reducing body weight and improving lipid profiles and circadian regulators possibly via the SCFAs/GPR43/GLP-1 pathway.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 7","pages":"1490-1497"},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The peptide toxin components and nucleotide metabolites in Macrothele raveni venom synergistically inhibit cancer cell proliferation by activating the pro-apoptotic pathways]. [大毒蛇毒液中的肽毒素成分和核苷酸代谢物通过激活促凋亡途径协同抑制癌细胞增殖]。
南方医科大学学报杂志 Pub Date : 2025-07-20 DOI: 10.12122/j.issn.1673-4254.2025.07.12
Ting Xie, Yunyun Wang, Ting Guo, Chunhua Yuan
{"title":"[The peptide toxin components and nucleotide metabolites in <i>Macrothele raveni</i> venom synergistically inhibit cancer cell proliferation by activating the pro-apoptotic pathways].","authors":"Ting Xie, Yunyun Wang, Ting Guo, Chunhua Yuan","doi":"10.12122/j.issn.1673-4254.2025.07.12","DOIUrl":"10.12122/j.issn.1673-4254.2025.07.12","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the inhibitory effect of <i>Macrothele raveni</i> crude venom against proliferation of different cancer cells and identify the active components in the venom.</p><p><strong>Methods: </strong>Different cancer cell lines were treated with different concentrations of <i>Macrothele raveni</i> venom for 48 h, and cell proliferation and the half-maximal inhibitory concentrations (IC<sub>50</sub>) of the venom were assessed with CCK-8 assay. The apoptosis rate of breast cancer MCF7 cells following the treatment was analyzed with flow cytometry, and the changes in cellular caspase-8 and caspase-9 expressions were detected. The crude venom was separated into protein, peptide, and small-molecule compound fractions using gel filtration chromatography and high-performance liquid chromatography (HPLC). The protein and peptide components were identified using proteomics analysis, and small-molecule compounds were structurally characterized using nuclear magnetic resonance (NMR), mass spectrometry (MS), and HPLC.</p><p><strong>Results: </strong>The crude venom exhibited strong concentration-dependent inhibitory effects on proliferation of MCF7 cells and nasopharyngeal carcinoma SUNE1 and HONE1 cells (IC<sub>50</sub> of 2.14±0.29, 1.57±0.14, and 2.85±0.15 µg/mL, respectively), with less potent inhibitory effects in gastric cancer HGC27 cells and colorectal cancer SW620 cells (IC<sub>50</sub> of 3.02±0.27 and 3.02±0.28 µg/mL, respectively). The crude venom significantly promoted MCF7 cell apoptosis likely <i>via</i> the caspase 8 signaling pathway. The protein fraction from the crude venom showed a weak inhibitory effect in MCF7 cells, whereas the peptide fraction exhibited a much stronger inhibitory effect (IC<sub>50</sub> of 6.41±0.31 µg/mL). The peptides in the peptide fraction, with relative molecular mass around 10 000, were homologous to those found in <i>Macrothele gigas</i> venom. The small-molecule fraction consisted mainly of nucleotide metabolites without obvious inhibitory effects in MCF7 cells, but its combination with the peptide fraction showed significantly enhanced inhibitory activity. C<b>onclusion</b> The inhibitory effects of <i>Macrothele raveni</i> venom, which vary significantly across different cancer cell lines, are attributed primarily to its peptide components, which may act synergistically with the nucleotide metabolites.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 7","pages":"1460-1470"},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The TGF‑β/miR-23a-3p/IRF1 axis mediates immune escape of hepatocellular carcinoma by inhibiting major histocompatibility complex class I]. [TGF - β/miR-23a-3p/IRF1轴通过抑制主要组织相容性复合体I类介导肝癌的免疫逃逸]。
南方医科大学学报杂志 Pub Date : 2025-07-20 DOI: 10.12122/j.issn.1673-4254.2025.07.06
Ying Yu, Li Tu, Yang Liu, Xueyi Song, Qianqian Shao, Xiaolong Tang
{"title":"[The TGF‑β/miR-23a-3p/IRF1 axis mediates immune escape of hepatocellular carcinoma by inhibiting major histocompatibility complex class I].","authors":"Ying Yu, Li Tu, Yang Liu, Xueyi Song, Qianqian Shao, Xiaolong Tang","doi":"10.12122/j.issn.1673-4254.2025.07.06","DOIUrl":"10.12122/j.issn.1673-4254.2025.07.06","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the mechanism by which transforming growth factor‑β (TGF‑β) regulates major histocompatibility complex class I (MHC-I) expression in hepatocellular carcinoma (HCC) cells and its role in immune evasion of HCC.</p><p><strong>Methods: </strong>HCC cells treated with TGF‑β alone or in combination with SB-431542 (a TGF-β type I receptor inhibitor) were examined for changes in MHC-I expression using RT-qPCR and Western blotting. A RNA interference experiment was used to explore the role of miR-23a-3p/IRF1 signaling in TGF‑β‑mediated regulation of MHC-I. HCC cells with different treatments were co-cultured with human peripheral blood mononuclear cells (PBMCs), and the changes in HCC cell proliferation was assessed using CCK-8 and colony formation assays. T-cell cytotoxicity in the co-culture systems was assessed with lactate dehydrogenase (LDH) release and JC-1 mitochondrial membrane potential assays, and T-cell activation was evaluated by flow cytometric analysis of CD69 cells and ELISA for TNF-α secretion.</p><p><strong>Results: </strong>TGF‑β treatment significantly suppressed MHC-I expression in HCC cells and reduced T-cell activation, leading to increased tumor cell proliferation and decreased HCC cell death in the co-culture systems. Mechanistically, TGF-β upregulated miR-23a-3p, which directly targeted IRF1 to inhibit MHC-I transcription. Overexpression of miR-23a-3p phenocopied TGF‑β‑induced suppression of IRF1 and MHC-I.</p><p><strong>Conclusions: </strong>We reveal a novel immune escape mechanism of HCC, in which TGF‑β attenuates T cell-mediated antitumor immunity by suppressing MHC-I expression through the miR-23a-3p/IRF1 signaling axis.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 7","pages":"1397-1408"},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Therapeutic mechanism of hederagenin, an active component in Guizhi Fuling Pellets, against cervical cancer in nude mice]. [桂枝茯苓丸有效成分皂苷元对裸鼠宫颈癌的作用机制]。
南方医科大学学报杂志 Pub Date : 2025-07-20 DOI: 10.12122/j.issn.1673-4254.2025.07.08
Yinfu Zhu, Yiran Li, Yi Wang, Yinger Huang, Kunxiang Gong, Wenbo Hao, Lingling Sun
{"title":"[Therapeutic mechanism of hederagenin, an active component in <i>Guizhi Fuling</i> Pellets, against cervical cancer in nude mice].","authors":"Yinfu Zhu, Yiran Li, Yi Wang, Yinger Huang, Kunxiang Gong, Wenbo Hao, Lingling Sun","doi":"10.12122/j.issn.1673-4254.2025.07.08","DOIUrl":"10.12122/j.issn.1673-4254.2025.07.08","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the therapeutic mechanism of <i>Guizhi Fuling</i> (GZFL) Pellets against cervical cancer.</p><p><strong>Methods: </strong>Publicly available databases were used to identify the targets of GZFL Pellets and cervical cancer to construct the protein-protein interaction (PPI) network, followed by GO biological process and KEGG pathway enrichment analysis of the hub genes. The \"Traditional Chinese Medicine-Active Ingredients-Targets-Pathways\" network for GZFL Pellets in cervical cancer treatment was generated using Cytoscape v10.0.0, and molecular docking of the drug and potential targets was performed to predict the specific targets of active components in <i>Guizhi Fuling</i> Pellets. The inhibitory effects of hederagenin, an active ingredient in GZFL Pellets, was tested in cultured cervical cancer cells and in nude mice bearing cervical cancer xenografts.</p><p><strong>Results: </strong>GZFL Pellets contain 338 active components targeting 247 action sites. A total of 10127 cervical cancer-related targets were obtained, and among them 195 were identified as potential therapeutic targets of GZFL Pellets for cervical cancer treatment, including the key targets of GABRA1, PTK2, JAK2, HTR3A, GSR, and IL-17. Molecular docking study showed low binding energies of the active components such as hederagenin, campesterol, and stigmasterol for protein-molecule interaction. GO enrichment analysis suggested that GZFL Pellets inhibited cervical cancer primarily by regulating responses to steroid hormones, oxidative stress, and lipopolysaccharides. Among the active components of GZFL Pellets, hederagenin was found to inhibit cervical cancer cells <i>in vitro</i> and significantly reduced STAT3 phosphorylation level in the cancer cells. In nude mice bearing cervical cancer xenografts, hederagenin effectively inhibited tumor growth rate without causing obvious adverse effects.</p><p><strong>Conclusions: </strong>GZFL Pellets inhibit cervical cancer cell growth through its multiple active components that target different pathways. Among these components, hederagenin inhibits tumor cell growth possibly by directly binding to JAK2 protein to inhibit STAT3 phosphorylation.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 7","pages":"1423-1433"},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Chaihu Shugan Decoction improves cognitive impairment after epilepsy in rats by regulating hippocampal NMDAR subunits via upregulating ASIC1]. [柴胡疏肝汤通过上调ASIC1调节海马NMDAR亚基改善癫痫大鼠认知功能障碍]。
南方医科大学学报杂志 Pub Date : 2025-07-20 DOI: 10.12122/j.issn.1673-4254.2025.07.17
Yunhong Yu, Wei Xie, Hui Li
{"title":"[<i>Chaihu Shugan</i> Decoction improves cognitive impairment after epilepsy in rats by regulating hippocampal NMDAR subunits <i>via</i> upregulating ASIC1].","authors":"Yunhong Yu, Wei Xie, Hui Li","doi":"10.12122/j.issn.1673-4254.2025.07.17","DOIUrl":"10.12122/j.issn.1673-4254.2025.07.17","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the therapeutic mechanism of <i>Chaihu Shugan</i> (CHSG) Decoction for improving cognitive impairment in rats with epilepsy induced by lithium chloride and pilocarpine.</p><p><strong>Methods: </strong>Male SD rat models of cognitive impairment model after epilepsy induced by intraperitoneal injection with lithium chloride and pilocarpine were randomly divided into 5 groups (<i>n</i>=12) for treatment with daily gavage of saline, donepezil (90 mg/kg), or CHSG Decoction at 2.5, 5.0, 10, 20 and 40 g/kg for 4 consecutive weeks, with 10 rats with intraperitoneal injection with saline as the blank control group. Morris water maze test was used to evaluate cognitive and behavioral changes of the rats after treatment. The mRNA and protein expressions of ASIC1, NR1, NR2A and NR2B in the hippocampus of rats were detected using RT-qPCR and Western blotting.</p><p><strong>Results: </strong>Compared with those with saline treatment, the rat models treated with CHSG Decoction at 5 and 10 g/kg showed significantly shortened escape latency and prolonged stay in the target quadrant with increased number of platform crossings in Morris water maze test. CHSG Decoction treatment at the two doses significantly increased ASIC1, NR1, NR2A and NR2B protein expressions in the hippocampus of the rat models, and their mRNA expression levels were all increased significantly after the treatment at the doses above 2.5 g/kg.</p><p><strong>Conclusions: </strong>CHSG Decoction can improve cognitive impairment in rats after epilepsy possibly by regulating the expression and channel activity of NMDAR protein and its subunit protein via upregulating ASIC1 to modulate neuronal excitability and synaptic plasticity in the hippocampus.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 7","pages":"1506-1512"},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Forensic performance and genetic background analyses of Guizhou Chuanqing population using a self-constructed microhaplotype panel]. 贵州川庆人群自构建微单倍型面板的法医鉴定及遗传背景分析
南方医科大学学报杂志 Pub Date : 2025-07-20 DOI: 10.12122/j.issn.1673-4254.2025.07.10
Hongling Zhang, Changyun Gu, Qiyan Wang, Xiaolan Huang, Qianchong Ran, Zheng Ren, Yubo Liu, Yansha Luo, Shuaiji Pan, Meiqing Yang, Jingyan Ji, Xiaoye Jin
{"title":"[Forensic performance and genetic background analyses of Guizhou Chuanqing population using a self-constructed microhaplotype panel].","authors":"Hongling Zhang, Changyun Gu, Qiyan Wang, Xiaolan Huang, Qianchong Ran, Zheng Ren, Yubo Liu, Yansha Luo, Shuaiji Pan, Meiqing Yang, Jingyan Ji, Xiaoye Jin","doi":"10.12122/j.issn.1673-4254.2025.07.10","DOIUrl":"10.12122/j.issn.1673-4254.2025.07.10","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the ethnic origin of Chuanqing people, one of the largest unidentified ethnic groups in Guizhou, China, and analyze its genetic relationships with surrounding populations.</p><p><strong>Methods: </strong>Based on a self-developed microhaplotype system, we conducted genotyping and analyzed the genetic distribution of microhaplotype loci and their forensic applicability in Chuanqing population in Guizhou Province. Using the microhaplotype data from different intercontinental populations and previously reported data from Han population living in Guizhou Province, we systematically investigated the genetic background of Chuanqing people through population genetic approaches, including genetic distance estimation, principal component analysis, and phylogenetic tree construction.</p><p><strong>Results: </strong>Among the studied population, the number of haplotype per microhaplotype ranged from 6 to 25. The average expected heterozygosity (He), observed heterozygosity (Ho), power of discrimination (PD), and probability of exclusion (PE) were 0.8291, 0.8301, 0.9387, and 0.6593, respectively. The cumulative power of discrimination (CPD) and cumulative probability of exclusion (CPE) for these 33 loci were 1-2.62×10<sup>-41</sup> and 1-7.64×10<sup>-17</sup>, respectively. Population genetic analyses revealed that the Chuanqing population had close genetic relationships with the East Asian populations, especially the local Guizhou Han population, Beijing Han population and the Han populations living in southern China.</p><p><strong>Conclusions: </strong>The 33 microhaplotypes exhibit high levels of genetic diversity in the Guizhou Chuanqing population, highlighting their potentials for both forensic identification and parentage testing. The Han populations might have contributed a significant amount of genetic material to the Chuanqing population during the formation and development of the latter.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 7","pages":"1442-1450"},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[LncRNA SNHG15 promotes proliferation, migration and invasion of lung adenocarcinoma cells by regulating COX6B1 through sponge adsorption of miR-30b-3p]. [LncRNA SNHG15通过海绵吸附miR-30b-3p调控COX6B1促进肺腺癌细胞的增殖、迁移和侵袭]。
南方医科大学学报杂志 Pub Date : 2025-07-20 DOI: 10.12122/j.issn.1673-4254.2025.07.16
Xiuying Gong, Shunfu Hou, Miaomiao Zhao, Xiaona Wang, Zhihan Zhang, Qinghua Liu, Chonggao Yin, Hongli Li
{"title":"[LncRNA SNHG15 promotes proliferation, migration and invasion of lung adenocarcinoma cells by regulating COX6B1 through sponge adsorption of miR-30b-3p].","authors":"Xiuying Gong, Shunfu Hou, Miaomiao Zhao, Xiaona Wang, Zhihan Zhang, Qinghua Liu, Chonggao Yin, Hongli Li","doi":"10.12122/j.issn.1673-4254.2025.07.16","DOIUrl":"10.12122/j.issn.1673-4254.2025.07.16","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the molecular mechanism by which lncRNA SNHG15 regulates proliferation, invasion and migration of lung adenocarcinoma cells.</p><p><strong>Methods: </strong>The lncRNA microarray chip dataset GSE196584 and LncBase were used to predict the lncRNAs that interact with miR-30b-3p, and their association with patient prognosis were investigated using online databases, after which lncRNA nucleolar RNA host gene 15 (SNHG15) was selected for further analysis. The subcellular localization of lncRNA SNHG15 and its expression levels in normal human lung epithelial cells and lung adenocarcinoma cell lines were detected using fluorescence in situ hybridization and qRT-PCR. In cultured A549 cells, the changes in cell proliferation, migration, and invasion following transfection with a SNHG15 knockdown plasmid (sh-SNHG15), a miR-30b-3p inhibitor, or their co-transfection were assessed with EdU, wound healing, and Transwell assays. Bioinformatics analyses were used to predict the regulatory relationship between lncRNA SNHG15 and COX6B1, and the results were verified using Western blotting and rescue experiments in A549 cells transfected with sh-SNHG15, a COX6B1-overexpressing plasmid, or both.</p><p><strong>Results: </strong>LncRNA SNHG15 was shown to target miR-30b-3p, and the former was highly expressed in lung adenocarcinoma, and associated with a poor patient prognosis. LncRNA SNHG15 was localized in the cytoplasm and expressed at higher levels in A549 and NCI-H1299 cells than in BEAS-2B cells. In A549 cells, lncRNA SNHG15 knockdown significantly inhibited cell migration, invasion and proliferation, and these changes were reversed by miR-30b-3p inhibitor. A regulatory relationship was found between lncRNA SNHG15 and COX6B1, and their expression levels were positively correlated (<i>r</i>=0.128, <i>P</i>=0.003). MiR-30b-3p knockdown obviously decreased COX6B1 expression in A549 cells, and COX6B1 overexpression rescued the cells from the inhibitory effects of lncRNA-SNHG15 knockdown.</p><p><strong>Conclusions: </strong>LncRNA SNHG15 may compete with COX6B1 to bind miR-30b-3p through a ceRNA mechanism to affect proliferation, migration, and invasion of lung adenocarcinoma cells.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 7","pages":"1498-1505"},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[A multi-feature fusion-based model for fetal orientation classification from intrapartum ultrasound videos]. 基于多特征融合的产时超声视频胎儿取向分类模型
南方医科大学学报杂志 Pub Date : 2025-07-20 DOI: 10.12122/j.issn.1673-4254.2025.07.24
Ziyu Zheng, Xiaying Yang, Shengjie Wu, Shijie Zhang, Guorong Lyu, Peizhong Liu, Jun Wang, Shaozheng He
{"title":"[A multi-feature fusion-based model for fetal orientation classification from intrapartum ultrasound videos].","authors":"Ziyu Zheng, Xiaying Yang, Shengjie Wu, Shijie Zhang, Guorong Lyu, Peizhong Liu, Jun Wang, Shaozheng He","doi":"10.12122/j.issn.1673-4254.2025.07.24","DOIUrl":"10.12122/j.issn.1673-4254.2025.07.24","url":null,"abstract":"<p><strong>Objectives: </strong>To construct an intelligent analysis model for classifying fetal orientation during intrapartum ultrasound videos based on multi-feature fusion.</p><p><strong>Methods: </strong>The proposed model consists of the Input, Backbone Network and Classification Head modules. The Input module carries out data augmentation to improve the sample quality and generalization ability of the model. The Backbone Network was responsible for feature extraction based on Yolov8 combined with CBAM, ECA, PSA attention mechanism and AIFI feature interaction module. The Classification Head consists of a convolutional layer and a softmax function to output the final probability value of each class. The images of the key structures (the eyes, face, head, thalamus, and spine) were annotated with frames by physicians for model training to improve the classification accuracy of the anterior occipital, posterior occipital, and transverse occipital orientations.</p><p><strong>Results: </strong>The experimental results showed that the proposed model had excellent performance in the tire orientation classification task with the classification accuracy reaching 0.984, an area under the PR curve (average accuracy) of 0.993, and area under the ROC curve of 0.984, and a kappa consistency test score of 0.974. The prediction results by the deep learning model were highly consistent with the actual classification results.</p><p><strong>Conclusions: </strong>The multi-feature fusion model proposed in this study can efficiently and accurately classify fetal orientation in intrapartum ultrasound videos.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 7","pages":"1563-1570"},"PeriodicalIF":0.0,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12268913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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