南方医科大学学报杂志最新文献

{"title":"[Activation of astrocytes in the dorsomedial hypothalamus accelerates sevoflurane anesthesia emergence in mice].","authors":"Shuting Guo, Fuyang Cao, Yongxin Guo, Yanxiang Li, Xinyu Hao, Zhuoning Zhang, Zhikang Zhou, Li Tong, Jiangbei Cao","doi":"10.12122/j.issn.1673-4254.2025.04.10","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.10","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the regulatory role of astrocytes in the dorsomedial hypothalamus (DMH) during sevoflurane anesthesia emergence.</p><p><strong>Methods: </strong>Forty-two male C57BL/6 mice were randomized into 6 groups (<i>n</i>=7) for assessing astrocyte activation in the dorsomedial hypothalamus (DMH) under sevoflurane anesthesia. Two groups of mice received microinjection of agfaABC1D promoter-driven AAV2 vector into the DMH for GCaMP6 overexpression, and the changes in astrocyte activity during sevoflurane or air inhalation were recorded using calcium imaging. For assessing optogenetic activation of astrocytes, another two groups of mice received microinjection of an optogenetic virus or a control vector into the DMH with optic fiber implantation, and sevoflurane anesthesia emergence was compared using behavioral experiments. In the remaining two groups, electroencephalogram (EEG) recording during sevoflurane anesthesia emergence was conducted after injection of the hChR2-expressing and control vectors. Anesthesia induction and recovery were assessed by observing the righting reflex. EEG data were recorded under 2.0% sevoflurane to calculate the burst suppression ratio (BSR) and under 1.5% sevoflurane for power spectrum analysis. Immunofluorescence staining was performed to visualize the colocalization of GFAP-positive astrocytes with viral protein signals.</p><p><strong>Results: </strong>Astrocyte activity in the DMH decreased progressively as sevoflurane concentration increased. During 2.0% sevoflurane anesthesia, the mice injected with the ChR2-expressing virus exhibited a significantly shortened wake-up time (<i>P</i><0.05), and optogenetic activation of the DMH astrocytes led to a marked reduction in BSR (<i>P</i><0.001). Under 1.5% sevoflurane anesthesia, optogenetic activation resulted in a significant increase in EEG gamma power and a significant decrease in delta power in ChR2 group (<i>P</i><0.01).</p><p><strong>Conclusions: </strong>Optogenetic activation of DMH astrocytes facilitates sevoflurane anesthesia emergence but does not significantly influence anesthesia induction. These findings offer new insights into the mechanisms underlying anesthesia emergence and may provide a potential target for accelerating postoperative recovery and managing anesthesia-related complications.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"751-759"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Causal relationship between autoimmune diseases and aplastic anemia: A Mendelian randomization study].","authors":"Wenjie Li, Yaonan Hong, Rui Huang, Yuchen Li, Ying Zhang, Yun Zhang, Dijiong Wu","doi":"10.12122/j.issn.1673-4254.2025.04.23","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.23","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the causal associations between autoimmune diseases and aplastic anemia (AA) using Mendelian randomization analysis.</p><p><strong>Methods: </strong>Publicly available genome-wide association study (GWAS) data were utilized to obtain single nucleotide polymorphisms (SNPs) associated with autoimmune diseases and AA for analysis. The inverse variance weighted (IVW) method was employed as the primary analytical approach, with MR Egger, Weighted Mode, Weighted Median, and Simple Mode methods serving as complementary analyses. Heterogeneity and pleiotropy analyses were conducted using designated functions, and the robustness of Mendelian randomization results was assessed using leave-one-out analysis.</p><p><strong>Results: </strong>The two-sample Mendelian randomization analysis using the IVW method revealed significant positive causal associations of rheumatoid arthritis (OR=1.094, 95% <i>CI</i>: 1.023-1.170, <i>P</i>=0.009, adjusted <i>P</i>=0.042), systemic lupus erythematosus (OR=1.111, 95% <i>CI</i>: 1.021-1.208, <i>P</i>=0.015, adjusted <i>P</i>=0.036), Hashimoto thyroiditis (OR=1.206, 95% <i>CI</i>: 1.049-1.387, <i>P</i>=0.009, adjusted <i>P</i>=0.029), and Sicca syndrome (OR=1.173, 95% <i>CI</i>: 1.054-1.306, <i>P</i>=0.004, adjusted <i>P</i>=0.035) with AA, which was supported by the results from the Weighted Median method. Sensitivity analyses indicated no evidence of pleiotropy or heterogeneity, and leave-one-out analysis confirmed the robustness of the causal relationships. No direct evidence was found linking Graves' disease, ulcerative colitis, Crohn's disease, autoimmune hepatitis, primary biliary cholangitis, or primary sclerosing cholangitis with AA (<i>P</i>>0.05, adjusted <i>P</i>>0.05), indicating a lack of causal association. Reverse Mendelian randomization results and multiple corrections indicated that AA was not an influencing factor for autoimmune diseases (adjusted <i>P</i>>0.05).</p><p><strong>Conclusions: </strong>Our findings support at the genetic level that rheumatoid arthritis, systemic lupus erythematosus, Hashimoto thyroiditis, and Sicca syndrome are risk factors for AA, and confirm a causal association of the these 4 autoimmune diseases with an increased risk of AA.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"871-879"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A low-dose CT image restoration method based on central guidance and alternating optimization].","authors":"Xiaoyu Zhang, Hao Wang, Dong Zeng, Zhaoying Bian","doi":"10.12122/j.issn.1673-4254.2025.04.20","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.20","url":null,"abstract":"<p><strong>Objectives: </strong>We propose a low-dose CT image restoration method based on central guidance and alternating optimization (FedGP).</p><p><strong>Methods: </strong>The FedGP framework revolutionizes the traditional federated learning model by adopting a structure without a fixed central server, where each institution alternatively serves as the central server. This method uses an institution-modulated CT image restoration network as the core of client-side local training. Through a federated learning approach of central guidance and alternating optimization, the central server leverages local labeled data to guide client-side network training to enhance the generalization capability of the CT imaging model across multiple institutions.</p><p><strong>Results: </strong>In the low-dose and sparse-view CT image restoration tasks, the FedGP method showed significant advantages in both visual and quantitative evaluation and achieved the highest PSNR (40.25 and 38.84), the highest SSIM (0.95 and 0.92), and the lowest RMSE (2.39 and 2.56). Ablation study of FedGP demonstrated that compared with FedGP(w/o GP) without central guidance, the FedGP method better adapted to data heterogeneity across institutions, thus ensuring robustness and generalization capability of the model in different imaging conditions.</p><p><strong>Conclusions: </strong>FedGP provides a more flexible FL framework to solve the problem of CT imaging heterogeneity and well adapts to multi-institutional data characteristics to improve generalization ability of the model under diverse imaging geometric configurations.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"844-852"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037291/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Aurora-A overexpression promotes cervical cancer cell invasion and metastasis by activating the NF-κBp65/ARPC4 signaling axis].","authors":"Yaqing Yue, Zhaoxia Mu, Xibo Wang, Yan Liu","doi":"10.12122/j.issn.1673-4254.2025.04.19","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.19","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the regulatory effects of Aurora-A in regulating proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of cervical cancer cells and the role of actin-related protein 2/3 complex subunit 4 (ARPC4) in mediating its effects.</p><p><strong>Methods: </strong>The plasmids pCDH-NC, pCDH-Aurora-A, and shRNA-ARPC4 were used for inducing Aurora-A overexpression or ARPC4 knockdown in HeLa cells. The cells were divided into vector group, Aurora-A overexpression group, Aurora-A overexpression+ARPC4 knockdown group, and Aurora-A overexpression+NF‑κBp65 inhibitor group and transfected with the corresponding plasmids. The proliferation, colony-forming ability, migration and invasion of the treated Hela cells was evaluated using EdU immunofluorescence assay, crystal violet staining, scratch assay, Transwell assay, and Matrigel assay. Western blotting was performed to detect the changes in cellular expressions of EMT-related proteins and expression levels of NF-κBp65 and ARPC4.</p><p><strong>Results: </strong>The expression of ARPC4 was significantly decreased in HeLa cells with Aurora-A knockdown and increased in Aurora-A-overexpressing cells. Aurora-A overexpression obviously promoted proliferation, migration, and invasion abilities of HeLa cells, and these effects was significantly antagonized by ARPC4 knockdown. In Aurora-A-overexpressing cells, the phosphorylation level of NF-κBp65 and the expression level of ARPC4 were increased significantly, and application of the NF‑κBp65 inhibitor obviously lowered the expression level of ARPC4.</p><p><strong>Conclusions: </strong>Aurora-A overexpression upregulates the expression of ARPC4 by activating the NF-κBp65 signaling pathway, thereby promoting migration, invasion and EMT of HeLa cells.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"837-843"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Risk factors for overall postoperative complications in elderly patients undergoing gastrointestinal surgeries: a multicenter observational study].","authors":"Xuecai Lü, Yanhong Liu, Shiyi Han, Haoyun Zhang, Aisheng Hou, Zhikang Zhou, Likai Shi, Jie Gao, Jiangbei Cao, Hong Zhang, Weidong Mi","doi":"10.12122/j.issn.1673-4254.2025.04.08","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.08","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the risk factors of overall postoperative complications in elderly patients undergoing gastrointestinal surgeries.</p><p><strong>Methods: </strong>This study was conducted among a total of 1388 elderly patients, who underwent elective gastrointestinal surgeries at 17 centers across China between April, 2020 and April, 2022. The primary outcome was the incidence of postoperative complications within 30 days, including procedure-related, neuropsychiatric, respiratory, cardiovascular, and gastrointestinal complications as well as acute kidney injury. Baseline characteristics, preoperative psychological and functional status, intraoperative anesthesia and surgical factors, intraoperative medication, use of nerve block, and postoperative analgesia methods were compared between the patients experiencing one or more postoperative complications and those without complications. Univariate and multivariate logistic regression analyses were performed to identify the independent risk factors for postoperative complications. The relationship between postoperative acute pain and each type of complication were explored.</p><p><strong>Results: </strong>The incidence of overall postoperative complications was 50.8% (705/1388) in these patients. Multivariate analysis showed that age (OR: 1.026; 95% <i>CI</i>: 1.006-1.046), prognostic nutritional index (OR: 0.998; 95% <i>CI</i>: 0.997-1.000), preoperative EuroQol-5 dimensions score (OR: 0.094; 95% <i>CI</i>: 0.018-0.500), blood loss (OR: 1.002; 95% <i>CI</i>: 1.001-1.003), and acute postoperative pain (OR: 1.308; 95% <i>CI</i>: 1.033-1.657) were significantly associated with the occurrence of postoperative complications. Specifically, patients experiencing severe postoperative pain had a significantly higher incidence of neuropsychiatric (27.2% <i>vs</i> 19.8%), procedure-related (17.3% <i>vs</i> 10.2%), and cardiovascular complications (3.6% <i>vs</i> 1.7%).</p><p><strong>Conclusions: </strong>An advanced age, a low preoperative nutritional index, a poor quality of life score, a greater volume of intraoperative blood loss, and acute postoperative pain are independent risk factors for postoperative complications in elderly patients undergoing gastrointestinal surgeries. There is a significant association between acute postoperative pain and multi-system complications.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"736-743"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[High expression of CDKN3 promotes migration and invasion of gastric cancer cells by regulating the p53/NF-κB signaling pathway and inhibiting cell apoptosis].","authors":"Yi Zhang, Yu Shen, Zhiqiang Wan, Song Tao, Yakui Liu, Shuanhu Wang","doi":"10.12122/j.issn.1673-4254.2025.04.21","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.21","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the expression of CDKN3 in gastric cancer and its impact on prognosis of gastric cancer patients.</p><p><strong>Methods: </strong>We analyzed CDKN3 expression in clinical specimens from 114 gastric cancer patients and assessed its association with 5-year postoperative survival of the patients. GO and KEGG enrichment analyses were used to predict the biological function and possible mechanism of CDKN3. The effects of lentivirus-mediated CDKN3 knockdown on biological behaviors of gastric cancer cells were evaluated using Transwell assay, CCK-8 assay, TUNEL staining, flow cytometry, and Western blotting.</p><p><strong>Results: </strong>CDKN3 expression was significantly higher in gastric cancer tissues than in the adjacent tissues with significant correlations with CEA level, CA19-9 level, and T and N staging (<i>P</i><0.05). High CDKN3 expression was an independent risk factor affecting 5-year postoperative survival of the patients and predictive for long-term prognosis (<i>P</i><0.01). Enrichment analyses suggested a probable association of CDKN3 with apoptosis. In MGC-803 cells, CDKN3 knockdown significantly lowered migration and invasion capacities of the cells, while CDKN3 overexpression produced the opposite effects. TUNEL staining revealed a significantly lower level of cell apoptosis in gastric cancer tissues than in adjacent tissues (<i>P</i><0.01). CDKN3 knockdown obviously inhibited proliferation and increased apoptosis of MGC-803 cells. CDKN3 overexpression down-regulated the expressions of p53, p21 and Bax and up-regulated the expressions of p-p65 and Bcl-2.</p><p><strong>Conclusions: </strong>CDKN3 is highly expressed in gastric cancer tissues and affects patient prognosis. CDKN3 overexpression promotes proliferation, invasion and migration and suppressed apoptosis of gastric cancer cells possibly through the p53/NF-κB signaling pathway.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"853-861"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[4‑(Arylethyl)‑pyrrolo[2,3-d] pyrimidine improves post-traumatic stress disorder in mice by inhibiting mGluR5-regulated ERK1/2-SGK1 signaling pathway].","authors":"Cunbao He, Shaojie Yang, Guoqi Zhu","doi":"10.12122/j.issn.1673-4254.2025.04.12","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.12","url":null,"abstract":"<p><strong>Objectives: </strong>To observe the effect of 4-(arylethynyl)-pyrrolo[2,3-d] pyrimidine (10b) on post-traumatic stress disorder (PTSD)-like behaviors and ERK1/2-SGK1 signaling pathway in mice.</p><p><strong>Methods: </strong>C57BL/6 mouse models exposed to single prolonged stress (SPS) were treated with daily gavage of saline, 10b at low, moderate and high doses, or paroxetine for 14 days. The changes in PTSD-like behaviors of SPS mice with different treatments were observed using behavioral tests. Western blotting and immunofluorescence assay were used to detect the protein expression levels of mGluR5, p-ERK, and SGK1 in the hippocampus of the mice. Pathological changes in the liver and kidney tissues of the mice were examined using HE staining. Molecular docking and molecular dynamics analyses were employed to evaluate the binding stability between the compound 10b and mGluR5.</p><p><strong>Results: </strong>Compared to the normal control mice, the SPS mice exhibited obvious PTSD-like behaviors with increased hippocampal expressions of mGluR5 and p-ERK proteins and decreased SGK1 protein expression. Compound 10b significantly ameliorated behavioral abnormalities in SPS mice, inhibited mGluR5 expression, and reversed the dysregulation of p-ERK and SGK1. No obvious liver or kidney toxicity was observed after 10b treatment. Molecular docking and dynamics studies demonstrated a stable interaction between 10b and mGluR5.</p><p><strong>Conclusions: </strong>The compound 10b ameliorates PTSD-like behaviors induced by SPS in mice possibly by inhibiting mGluR5 expression to modulate the ERK1/2-SGK1 signaling pathway.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"765-773"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[<i>Tuihuang</i> Mixture improves α‑naphthylisothiocyanate-induced cholestasis in rats by inhibiting NLRP3 inflammasomes <i>via</i> regulating farnesoid X receptor].","authors":"Zhengwang Zhu, Linlin Wang, Jinghan Zhao, Ruixue Ma, Yuchun Yu, Qingchun Cai, Bing Wang, Pingsheng Zhu, Mingsan Miao","doi":"10.12122/j.issn.1673-4254.2025.04.06","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.06","url":null,"abstract":"<p><strong>Objectives: </strong>To study the therapeutic mechanism of <i>Tuihuang</i> Mixture against cholestasis.</p><p><strong>Methods: </strong>Forty-eight Wistar rats were randomized equally into blank group, model group, ursodeoxycholic acid group and <i>Tuihuang</i> Mixture group. Except for those in the blank group, all the rats were given α‑naphthylisothiocyanate (ANIT) to establish rat models of cholestasis, followed by treatments with indicated drugs or distilled water. Serum levels of ALT, AST, ALP, γ-GT, TBA and TBIL of the rats were determined, and hepatic expressions IL-1β, IL-18, FXR, NLRP3, ASC, Caspase-1 and GSDMD were detected using q-PCR, ELISA or Western blotting. Histopathological changes of the liver tissues were observed using HE staining.</p><p><strong>Results: </strong>The rat models of cholestasis had significantly increased serum levels of ALT, AST, ALP, γ-GT, TBA and TBIL with increased mRNA and protein expressions of IL-1β and IL-18, decreased protein and mRNA expressions of FXR, and increased protein expressions of NLRP3 and Caspase-1 and mRNA expressions of NLRP3, ASC, Caspase-1 and GSDMD in the liver tissue, showing also irregular arrangement of liver cells, proliferation of bile duct epithelial cells and inflammatory cells infiltration. Treatment of the rat models with <i>Tuihuang</i> Mixture significantly decreased serum levels of ALT, AST, ALP, γ-GT, TBA and TBIL, lowered IL-1β and IL-18 and increased FXR protein and mRNA expressions, and reduced NLRP3, ASC, Caspase-1 and GSDMD proteins and NLRP3, ASC and Caspase-1 mRNA expressions in the liver tissue. <i>Tuihuang</i> Mixture also significantly alleviated hepatocyte injury, bile duct epithelial cell proliferation and inflammatory cell infiltration in the liver of the rat models.</p><p><strong>Conclusions: </strong><i>Tuihuang</i> Mixture can effectively improve cholestasis in rats possibly by inhibiting NLRP3 inflammatosome-mediated pyroptosis via regulating FXR.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"718-724"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A cardiac magnetic resonance-based risk prediction model for left ventricular adverse remodeling following percutaneous coronary intervention for acute ST-segment elevation myocardial infarction: a multi-center prospective study].","authors":"Zhenyan Ma, Xin A, Lei Zhao, Hongbo Zhang, Ke Liu, Yiqing Zhao, Geng Qian","doi":"10.12122/j.issn.1673-4254.2025.04.01","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.01","url":null,"abstract":"<p><strong>Objectives: </strong>To develop a risk prediction model for left ventricular adverse remodeling (LVAR) based on cardiac magnetic resonance (CMR) parameters in patients undergoing percutaneous coronary intervention (PCI) for acute ST-segment elevation myocardial infarction (STEMI).</p><p><strong>Methods: </strong>A total of 329 acute STEMI patients undergoing primary PCI at 8 medical centers from January, 2018 to December, 2021 were prospectively enrolled. The parameters of CMR, performed at 7±2 days and 6 months post-PCI, were analyzed using CVI42 software. LVAR was defined as an increase >20% in left ventricular end-diastolic volume or >15% in left ventricular end-systolic volume at 6 months compared to baseline. The patients were randomized into training (<i>n</i>=230) and validation (<i>n</i>=99) sets in a 7∶3 ratio. In the training set, potential predictors were selected using LASSO regression, followed by univariate and multivariate logistic regression to construct a nomogram. Model performance was evaluated using receiver-operating characteristic (ROC) curves, area under the curve (AUC), calibration curves, and decision curve analysis.</p><p><strong>Results: </strong>LVAR occurred in 100 patients (30.40%), who had a higher incidence of major adverse cardiovascular events than those without LVAR (58.00% <i>vs</i> 16.16%, <i>P</i><0.001). Left ventricular global longitudinal strain (LVGLS; OR=0.76, 95% <i>CI</i>: 0.61-0.95, <i>P</i>=0.015) and left atrial active strain (LAAS; OR=0.78, 95% <i>CI</i>: 0.67-0.92, <i>P</i>=0.003) were protective factors for LVAR, while infarct size (IS; OR=1.05, 95% <i>CI</i>: 1.01-1.10, <i>P</i>=0.017) and microvascular obstruction (MVO; OR=1.26, 95% <i>CI</i>: 1.01-1.59, <i>P</i>=0.048) were risk factors for LVAR. The nomogram had an AUC of 0.90 (95% <i>CI</i>: 0.86-0.94) in the training set and an AUC of 0.88 (95% <i>CI</i>: 0.81-0.94) in the validation set.</p><p><strong>Conclusions: </strong>LVGLS, LAAS, IS, and MVO are independent predictors of LVAR in STEMI patients following PCI. The constructed nomogram has a strong predictive ability to provide assistance for management and early intervention of LVAR.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"669-683"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Bioinformatics analysis of oxidative stress and immune infiltration in rheumatoid arthritis].","authors":"Zhi Gao, Ao Wu, Zhongxiang Hu, Peiyang Sun","doi":"10.12122/j.issn.1673-4254.2025.04.22","DOIUrl":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.22","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the role of oxidative stress and immune infiltration in rheumatoid arthritis (RA).</p><p><strong>Methods: </strong>RA datasets GSE55235 (10 RA <i>vs</i> 10 normal samples) and GSE55457 (13 RA <i>vs</i> 10 normal samples) from the GEO database were merged as the test set to identify the differentially expressed genes (DEGs) in RA using R. The DEGs were intersected with oxidative stress-related genes to obtain oxidative stress-associated DEGs. KEGG and GO enrichment analyses of the DEGs were performed, and the RA-related pathways and biological processes were analyzed using GSEA. A protein-protein interaction (PPI) network was constructed using STRING and Cytoscape, and the top 10 key genes were obtained using the Degree algorithm. The validation dataset GSE1919 from GEO database was used for ROC analysis of the key genes to obtain the core genes, and their correlations with infiltrating immune cells were analyzed using CIBERSORT. The results were verified by RT-qPCR for detecting expression levels of the core genes in RA and normal joint samples.</p><p><strong>Results: </strong>We identified 89 oxidative stress-associated DEGs. Enrichment analysis suggested that these DEGs were involved in the biological processes including oxidative stress, chemical stress response, reactive oxygen species response, and lipopolysaccharide response. ROC analysis showed that the 5 core genes (STAT1, MMP9, MYC, CCL5, and JUN) all had AUC values >0.7, indicating their high diagnostic sensitivity and specificity for RA. These genes were closely correlated with immune cells, particularly T cells. RT-qPCR confirmed significant differential expressions of the core genes between RA and normal samples.</p><p><strong>Conclusions: </strong>Oxidative stress and diverse immune responses are features of RA, and the immune responses contribute to activation of oxidative stress. The identified core genes can potential serve as new diagnostic markers for RA.</p>","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"862-870"},"PeriodicalIF":0.0,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}