[Cannabidiol regulates circadian rhythm to improve sleep disorders following general anesthesia in rats].

Q3 Medicine

南方医科大学学报杂志 Pub Date : 2025-04-20 DOI:10.12122/j.issn.1673-4254.2025.04.09

Xinshun Wu, Jingcao Li, Ying Liu, Renhong Qiu, Henglin Wang, Rui Xye, Yang Zhang, Shuo Li, Qiongyin Fan, Huajin Dong, Youzhi Zhang, Jiangbei Cao

{"title":"[Cannabidiol regulates circadian rhythm to improve sleep disorders following general anesthesia in rats].","authors":"Xinshun Wu, Jingcao Li, Ying Liu, Renhong Qiu, Henglin Wang, Rui Xye, Yang Zhang, Shuo Li, Qiongyin Fan, Huajin Dong, Youzhi Zhang, Jiangbei Cao","doi":"10.12122/j.issn.1673-4254.2025.04.09","DOIUrl":null,"url":null,"abstract":"Objectives: To assess the regulatory effect of cannabidiol (CBD) on circadian rhythm sleep disorders following general anesthesia and explore its potential mechanism in a rat model of propofol-induced rhythm sleep disorder.Methods: An electrode was embedded in the skull for cortical EEG recording in 24 male SD rats, which were randomized into control, propofol, CBD treatment, and diazepam treatment groups (n=6). Eight days later, a single dose of propofol (10 mg/kg) was injected via the tail vein with anesthesia maintenance for 3 h in the latter 3 groups, and daily treatment with saline, CBD or diazepam was administered via gavage; the control rats received only saline injection. A wireless system was used for collecting EEG, EMG, and body temperature data within 72 h after propofol injection. After data collection, blood samples and hypothalamic tissue samples were collected for determining serum levels of oxidative stress markers and hypothalamic expressions of the key clock proteins.Results: Compared with the control rats, the rats with CBD treatment showed significantly increased sleep time at night (20:00-6:00), especially during the time period of 4:00-6:00 am. Compared with the rats in propofol group, which had prolonged SWS time and increased sleep episodes during 18:00-24:00 and sleep-wake transitions, the CBD-treated rats exhibited a significant reduction of SWS time and fewer SWS-to-active-awake transitions with increased SWS aspects and sleep-wake transitions at night (24:00-08:00). Diazepam treatment produced similar effect to CBD but with a weaker effect on sleep-wake transitions. Propofol caused significant changes in protein expressions and redox state, which were effectively reversed by CBD treatment.Conclusions: CBD can improve sleep structure and circadian rhythm in rats with propofol-induced sleep disorder possibly by regulating hypothalamic expressions of the key circadian clock proteins, suggesting a new treatment option for perioperative sleep disorders.","PeriodicalId":18962,"journal":{"name":"南方医科大学学报杂志","volume":"45 4","pages":"744-750"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037286/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"南方医科大学学报杂志","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12122/j.issn.1673-4254.2025.04.09","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: To assess the regulatory effect of cannabidiol (CBD) on circadian rhythm sleep disorders following general anesthesia and explore its potential mechanism in a rat model of propofol-induced rhythm sleep disorder.

Methods: An electrode was embedded in the skull for cortical EEG recording in 24 male SD rats, which were randomized into control, propofol, CBD treatment, and diazepam treatment groups (n=6). Eight days later, a single dose of propofol (10 mg/kg) was injected via the tail vein with anesthesia maintenance for 3 h in the latter 3 groups, and daily treatment with saline, CBD or diazepam was administered via gavage; the control rats received only saline injection. A wireless system was used for collecting EEG, EMG, and body temperature data within 72 h after propofol injection. After data collection, blood samples and hypothalamic tissue samples were collected for determining serum levels of oxidative stress markers and hypothalamic expressions of the key clock proteins.

Results: Compared with the control rats, the rats with CBD treatment showed significantly increased sleep time at night (20:00-6:00), especially during the time period of 4:00-6:00 am. Compared with the rats in propofol group, which had prolonged SWS time and increased sleep episodes during 18:00-24:00 and sleep-wake transitions, the CBD-treated rats exhibited a significant reduction of SWS time and fewer SWS-to-active-awake transitions with increased SWS aspects and sleep-wake transitions at night (24:00-08:00). Diazepam treatment produced similar effect to CBD but with a weaker effect on sleep-wake transitions. Propofol caused significant changes in protein expressions and redox state, which were effectively reversed by CBD treatment.

Conclusions: CBD can improve sleep structure and circadian rhythm in rats with propofol-induced sleep disorder possibly by regulating hypothalamic expressions of the key circadian clock proteins, suggesting a new treatment option for perioperative sleep disorders.

查看原文本刊更多论文

[大麻二酚调节昼夜节律改善大鼠全身麻醉后的睡眠障碍]。

目的：评价大麻二酚（CBD）对全身麻醉后昼夜节律性睡眠障碍的调节作用，并探讨其在异丙酚诱导的节律性睡眠障碍大鼠模型中的作用机制。方法：选取24只雄性SD大鼠，随机分为对照组、异丙酚组、CBD组和地西泮组（n=6）。8 d后，后3组经尾静脉注射单剂量异丙酚（10 mg/kg），维持麻醉3 h，每日灌胃生理盐水、CBD或地西泮；对照组只注射生理盐水。使用无线系统采集注射异丙酚后72h内的脑电图、肌电图和体温数据。数据收集后，采集血液样本和下丘脑组织样本，测定血清氧化应激标志物水平和下丘脑关键时钟蛋白的表达。结果：与对照大鼠相比，CBD处理大鼠夜间（20:00-6:00）睡眠时间明显增加，尤其是凌晨4:00-6:00时段。与异丙酚组相比，在18:00-24:00和睡眠-觉醒转换期间，大鼠的SWS时间延长，睡眠发作次数增加，而cbd处理的大鼠在夜间（24:00-08:00）的SWS方面和睡眠-觉醒转换增加，SWS时间明显减少，swws -active-awake转换减少。地西泮治疗产生了与CBD相似的效果，但对睡眠-觉醒转换的影响较弱。异丙酚引起了蛋白表达和氧化还原状态的显著变化，CBD有效地逆转了这一变化。结论：CBD可能通过调节下丘脑关键生物钟蛋白的表达来改善异丙酚诱导的睡眠障碍大鼠的睡眠结构和昼夜节律，为围手术期睡眠障碍的治疗提供了新的选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊