Nanomedicine Journal最新文献

{"title":"Barriers and recent advances in non-viral vectors targeting the lungs for cystic fibrosis gene therapy","authors":"Faisal Qaisar, Anum Habib, Maira Riaz, Z. Rehman","doi":"10.22038/NMJ.2019.06.0001","DOIUrl":"https://doi.org/10.22038/NMJ.2019.06.0001","url":null,"abstract":"Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in CFTR genes that affect chloride ion channel. The CF is a good nominee for gene therapy as the asymptomatic carriers are phenotypically normal, and the desired cells are accessible for vector delivery. Gene therapy shows promising effects involving the correction of gene or replacement of the mutant gene with the functional one. Accordingly, various viral and non-viral carriers have been investigated. Although viral vectors are efficient, they have some problems, including mutagenesis, host immune response, higher toxicity, and costliness. On the other hand, non-viral vectors have less toxicity and immunogenic response and are easier to prepare. For a successful gene therapy, the cargo must be delivered to the target site. However, various barriers are faced by non-viral vectors, which make the gene delivery to the target site difficult. Extracellular barrier, which is the first barrier, include nucleases, negatively charged serum proteins, blood cells, and activated immune system. Ciliated epithelium, mucus gel, apical surface glycocalyx, and plasma membrane come in the second category of the barriers. Furthermore, the third category, which is related to the intracellular barriers, includes endosome and lysosome, cytoplasmic nucleases, viscous environment of cytoplasm with different proteins, and finally nuclear membrane. Various approaches have been proposed to increase the systematic delivery of vectors and enhance their efficiency. Some of these approaches include surface coating with inert polymers, modification of surface charge with anionic polymers, and enhancement of endocytosis and reduction of toxicity by using polyethylene glycol. This review paper was conduct to highlight the barriers faced by non-viral vectors when carrying a genetic payload to the lungs. This study also involved the investigation of the strategies and different types of modifications targeted toward the improvement of the efficiency of non-viral vectors.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42873816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of CuO/Epoxy nanocomposites for the preparation of antifungal coating","authors":"Shima Nazarzade, H. Ghorbani","doi":"10.22038/NMJ.2019.06.0009","DOIUrl":"https://doi.org/10.22038/NMJ.2019.06.0009","url":null,"abstract":"Objective(s): Antibacterial and antifungal nanocomposites are widely used in food packaging and pharmaceutical and medicine industries. Among the polymers of these nanocomposites, epoxy coatings are commonly used for health and industrial applications. The present study aimed to synthesize CuO nanoparticles using the chemical reduction method and characterized them by ultraviolet-visible (UV-Vis) spectroscopy and dynamic light scattering (DLS) analysis. Materials and Methods: The nanoparticles were synthesized with the mean size of 45 nanometers. Following that, the CuO/epoxy nanocomposite were prepared in three concentrations of 1%, 3%, and 5% of the CuO nanoparticles. The results of X-ray diffractometry (XRD) and scanning electron microscopy (SEM) confirmed the presence of nanoparticles on the nanocomposite surface. In addition, the disc-diffusion method was used to assess the antifungal properties of the nanocomposites. Results: The results of XRD and SEM confirmed the presence of CuO nanoparticles on the nanocomposite surface. The optimal nanocomposite concentration for the maximum antifungal activity was 3%.Conclusion: It seems that the CuO nanoparticles could be used to provide antifungal nanocomposites, which are applicable in medicine and food industries.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42182831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entrapped chemically synthesized gold nanoparticles combined with polyethylene glycol and chloroquine diphosphate as an improved antimalarial drug","authors":"Shittu Oluwatosin Kudirat, A. Tawakalitu, A. A. Saka, Abubakre O. Kamaldeen, T. BankoleMercy, T. Oladejo","doi":"10.22038/NMJ.2019.06.0002","DOIUrl":"https://doi.org/10.22038/NMJ.2019.06.0002","url":null,"abstract":"Objective(s): Drug delivery is an engineering technology to control the release and delivery of therapeutic agents to target organs, tissues, and cells. Metallic nanoparticles, such as gold nanoparticles (AuNPs) have exceptional properties which enable efficient drug transport into different cell types with reduced side effects and cytotoxicity to other tissues.Materials and Methods: AuNPs were synthesized by adopting the Turkevich method to reduce tetra chloroauric (III) acid (HAuCl4) solution with sodium citrate. A factorial design of 24 was used to investigate the influence of temperature, stirring speed, and the volume of citrate and gold salt on the size of AuNPs synthesis. The produced chemical-AuNPs (CN-AuNPs) were characterized using ultraviolet-visible spectroscopy and dynamic light scattering (DLS) which was conjugated with polyethylene glycol (PEG) loaded with chloroquine diphosphate. The latter were characterized with transmission electron microscopy (TEM), Energy dispersive x-ray spectroscopy (EDS), selected area electron diffraction (SAED) patterns and Fourier transmission infrared spectroscopy. The antimalarial activities of the three formulations were tested on Plasmodium-infected mice. Moreover, the evaluation of curative potentials of the formulations was carried out via parasite counts. The anemic and pathological conditions of nano-encapsulation were investigated for their cytotoxicity level. Results: The CN-AuNPs show surface plasmon resonance absorption ranging from 526 to 529 nm with smaller particle size at the lower citrate volume. The TEM image of CN-AuNPs with polyethylene glycol (PEG) and CN-AuNPs-PEG encapsulated with chloroquine diphosphate revealed spherical shape with EDS showing the appearance of gold (Au) at 2.0, 2.1, and 9.9 KeV. The SAED also revealed that the AuNPs were crystalline in nature. The in vitro time-dependent encapsulation release showed an extension of time release, compared to CN-AuNPs-PEG with parasitemia clearance at the same level of cytotoxicity. Conclusion: Therefore, although improved activity of the CN-AuNPs-PEG encapsulating was achieved but its cytotoxicity still is a limitation.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49387941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A colorimetric aptasensor for selective detection of oxytetracycline in milk, using gold nanoparticles and oxytetracline-short aptamer","authors":"Hanif Kazerooni, A. Bahreyni, M. Ramezani, K. Abnous, M. Taghdisi","doi":"10.22038/NMJ.2019.06.0004","DOIUrl":"https://doi.org/10.22038/NMJ.2019.06.0004","url":null,"abstract":"Objective (s): In light of misuse of antibiotics in animal husbandry and their side effects on human health, there is an argent need to develop simple and rapid methods for determining the quantification of antibiotics in biological systems. Materials and Methods: In this work a facile and ultrasensitive colorimetric aptasensor was reported for detection of oxytetracycline (OTC) in water and milk samples employing OTC-short aptamer and gold nanoparticles (AuNPs). Results: In the presence of OTC, the interaction between OTC and its aptamer leads to the separation of OTC aptamer from the surface of AuNPs which is followed by the aggregation of AuNPs by salt, showing an evident color change from red to blue. On the contrary, in the absence of OTC, the attachment of aptamer on the surface of AuNPs can protect AuNPs against salt-induced aggregation with a wine-red color. The proposed aptasensor exhibits excellent sensitivity for detection of OTC with linear range between 20 to 2000 nM with limit of detection (LOD) as low as 10 nM. Furthermore, this strategy was applied to detect OTC in spiked milk samples and presented satisfying linear range from 25 to 1500 nM with the LOD of 20 nM. Conclusion: Owing to demonstrating appropriate sensitivity and selectivity, the designed biosensor can be considered as a promising tool to be applied in the field of biomedicine and food safety.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45138436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective and modulatory effects of royal jelly used against the induced changes in silver nanoparticles on the hippocampus of male rats","authors":"Mahsa Dalfardi, M. Taghavi, M. S. Kohbanani, Z. Taghipour, Reza Nosratabadi, Reza Nosratabadi, C. Jalili, M. Salahshoor, A. Kaeidi, A. Shabanizadeh","doi":"10.22038/NMJ.2019.06.0008","DOIUrl":"https://doi.org/10.22038/NMJ.2019.06.0008","url":null,"abstract":"Objective (s): Silver nanoparticles (NPs) have attracted considerable attention owing to their important properties, including antimicrobial and anti-oxidative stress effects. However, high concentrations of silver NPs have been reported to have toxic effects. The present study aimed to evaluate the modulatory and protective effects of royal jelly (RJ) against the harmful effects of silver NPs on hippocampal functions, such as learning and memory. Materials and Methods: This experimental study was conducted on 40 male Wistar rats. The animals were divided into four groups of 10, including the control group (no silver NPs and RJ), RJ group, silver NPs plus RJ, and silver NPs. Some functions of the hippocampus (e.g., learning and memory) were evaluated using Morris memory function tests for four consecutive days. In addition, the relative expression of TRPV1 was assessed using real-time polymerase chain reaction (RT-PCR). At the final stage, hippocampal tissues were collected for histological studies.Results: Levels of learning and memory, relative gene expression ratio of TRPV1, and the histological changes in the hippocampus were significantly different in the groups receiving silver NPs compared to the groups administered with RJ. Conclusion: According to the results, RJ may be the effective in the protection against the adverse effects of silver NPs and improve the function of the hippocampus.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46653174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the effects of silver nanoparticles and silver cobalt nanoparticles on function tests and liver tissue changes in adult male rats","authors":"Z. Parang, M. Parsaeian, D. Moghadamnia","doi":"10.22038/NMJ.2019.06.0007","DOIUrl":"https://doi.org/10.22038/NMJ.2019.06.0007","url":null,"abstract":"Objective(s): This study aimed to compare impacts of silver nanoparticles and silver cobalt nanoparticles on the hepatic function tests and changes in liver tissues in adult male rats.Materials and Methods: This study was conducted on 49 adult male Wistar rats, each weighing approximately 180-220 gr. The rats were randomly assigned to seven groups of seven including one control group and six experimental groups. The experimental groups 1 and 2 respectively received 25 and 100 mg/kg of silver nanoparticles synthesized for 75 sec intraperitoneally for 14 days. Experimental group 3 received silver nanoparticles that were synthesized at 300 sec which were administered intraperitoneally in a 25 mg/kg dose for 14 days. The experimental groups 4 and 5 received silver cobalt nanoparticles, whereby silver nanoparticles were synthesized at 75 sec and were administered intraperitoneally in a 25 and 100 mg/kg dose for 14 days, respectively. Finally, experimental group 6 received a 25 mg/kg dose of silver cobalt nanoparticles, intraperitoneally for 14 days, with the silver nanoparticles synthesized for 300 sec. At the end of this period, the serum levels of hepatic enzymes, albumin, and total protein were measured and tissue changes were evaluated in this study.Results: The mean serum levels of AST, total protein, and albumin in the experimental groups 1 and 3 increased significantly compared to the control group. Similarly, the mean serum levels of ALT and ALP in the experimental group 3 showed a significant increase in comparison with the control group. The mean of liver weight in all experimental groups was significantly higher than the control group(P<0.05). Furthermore, the necrosis of the liver tissue was observed in the recipients of silver nanoparticles. However, liver necrosis was not observed in the groups receiving silver cobalt nanoparticles.Conclusion: The use of silver nanoparticles can boost the serum levels of hepatic enzymes and increase liver tissue necrosis, as well. However, the silver cobalt nanoparticles did not change the levels of hepatic enzymes and liver tissue.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46974772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The comparison of the apoptosis effects of titanium dioxide nanoparticles into MDA-MB-231 cell line in microgravity and gravity conditions","authors":"A. Hekmat, M. Rabizadeh, M. Safavi, Z. Hajebrahimi","doi":"10.22038/NMJ.2019.06.0006","DOIUrl":"https://doi.org/10.22038/NMJ.2019.06.0006","url":null,"abstract":"Objective (s): Gravity could affect some system features and perform directly as an organizing field factor. Recent investigations have examined the titanium dioxide nanoparticles (TiO2 NPs) in biomedical applications, mostly in the cancer treatment field. This study aimed to evaluate the effects of simulated microgravity combined with TiO2 NPs in MDA-MB-231 cells proliferation for the first time. In other words, this study examined the utility of the microgravity environment in nano-therapy. Materials and Methods: The MDA-MB-231 human breast cancer cell line and TiO2 NPs were purchased. The 2D clinostat was applied for the simulation of the microgravity. The morphological studies, MTT cytotoxicity assay, Acridine orange/Ethidium bromide double staining studies and flow cytometry analysis were utilized.Results: The MTT assay, the morphological studies, Acridine orange/Ethidium bromide double staining studies and flow cytometry analysis confirmed the apoptosis-inducing effect of microgravity in combination with TiO2 NPs. The IC50 of simulated microgravity in the presence of TiO2 NPs was determined to be 130 µM. Furthermore, MDA-MB-231 cells exposed to microgravity adopted a different phenotype. Conclusion: Based on our observation, although the relative mechanisms need to be explored further, microgravity can strictly affect the TiO2 NPs effects on MDA-MB-231 cells. The significance of this study lied in the fact that simulating microgravity can be a powerful physical cure for cancer therapy and open new horizons for the studies in the field of biology, biophysics, and medicine.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49519270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on the applications of nanotechnology in orthodontics","authors":"S. Tahmasbi, Fatemeh Mohamadian, S. Hosseini, L. Eftekhar","doi":"10.22038/NMJ.2019.06.002","DOIUrl":"https://doi.org/10.22038/NMJ.2019.06.002","url":null,"abstract":"Objective (s): Nanotechnology has gained importance in recent years due to its ability in the enhancement of materials properties and other specifications such as antimicrobial properties. Nano-sized materials have been applied in various fields of dentistry. Nanotechnology can be employed in orthodontics to enhance the quality of treatment. In the current study, a comprehensive review is carried out on the applications of nanotechnology in orthodontics. Materials and Methods: In the first step, various databases such as Scopus, Google Scholar and Pubmed were searched by using appropriate keywords for the present study. Afterwards, the related resources were selected to be reviewed. Finally, the key findings of the reviewed studies were represented and summarized. Results: Based on the reviewed researches, nanotechnology is applicable in various aspects of orthodontics. By using nanotechnology, improved properties in mechanical and medical specifications are achievable. For instance, by using nano coating in archwires, the friction force between components can be reduced and facilitate its motion. In addition, adding some types of nano particles to the composites resulted in improvement in tensile and shear bond strength. Antimicrobial properties of specific nano particles such as silver makes them favorable for reducing microorganisms in orthodontics treatment. Moreover, nanotechnology can be used in nano-identation test to assess the tools employed in orthodontics. Conclusion: nanotechnology can be broadly employed in orthodontics to achieve better treatment including improved strength of utilized materials, more accurate positioning and reduced microorganisms.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68369207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocurcumin as a radioprotective agent against radiation-induced mortality in mice","authors":"R. Sadeghi, A. Razzaghdoust, Mohsen Bakhshandeh, F. Nasirinezhad, B. Mofid","doi":"10.22038/NMJ.2019.06.006","DOIUrl":"https://doi.org/10.22038/NMJ.2019.06.006","url":null,"abstract":"Objective(s): Curcumin, a natural plant product, is commonly known as wonder drug of life, but the poor bioavailability of its free form has hindered its clinical development. The aim of the present study was to investigate the radioprotective effect of nanocurcumin on survival of mice under whole body X-ray irradiation. Materials and Methods: The Naval Medical Research Institute (NMRI) mice randomly assigned to separate groups and received nanocurcumin via oral gavage at different time points related to irradiation. The survival of mice was evaluated daily for 30 days post-irradiation and finally, the LD50/30 was calculated using Probit analysis. The 30-day survival curve was plotted using the Kaplan-Meier survival curve and the median survival of different subgroups was compared using log-rank test. The P-values less than 0.05 were considered significant. Results: Our results showed that the administration of oral nanocurcumin could effectively reduce the mortality rate in the irradiated mice. Five days pretreatment with nanocurcumin (4 mg/kg/day) induced maximum radioprotective effect. The LD50/30 was 7.18 Gray (Gy) (95% confidence interval [CI]: 6.59-7.77) and 8.78 Gy (95% CI: 8.14-9.50) for irradiation-only and the optimum nanocurcumin group (pre-irradiation group), respectively (dose reduction factor [DRF] = 1.22). Continued administration of nanocurcumin up to seven days post-irradiation resulted in no further radioprotection. Conclusions: The results obtained in this study confirmed the efficacy of nanocurcumin as a radioprotective agent against radiation-induced mortality in mice. The specific characteristics of nanocurcumin, such as non-toxicity, edibility, availability, make this phytochemical as a potential radioprotective agent in the radiotherapy setting and radiation accidents. Further clinical studies are highly recommended.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68369907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposome and polymer-based nanomaterials for vaccine applications","authors":"P. Roopngam","doi":"10.22038/NMJ.2018.06.001","DOIUrl":"https://doi.org/10.22038/NMJ.2018.06.001","url":null,"abstract":"Nanoparticles (NPs) are effective and safe adjuvants for antigen delivery in modern vaccinology. Biodegradable nanomaterials with suitable properties are frequently applied for conjugation or loading with antigens; they protect the antigens from degradation in vivo. NPs are applied as effective delivery system to facilitate antigen uptake by antigen presenting cells (APCs) and especially dendritic cells (DCs) both in vitro and in vivo. Using nanoparticles to target DCs is an effective method to deliver antigens and potent immunomodulators. Uptake of NPs by DCs enhances the intracellular process of antigens and the antigen presentation pathway by MHC class I and II molecules to induce both CD4+ and CD8+ T-cell responses. Liposome and polymer-based NPs are now extensively applied as effective adjuvants or immunomodulators in several types of vaccines. In this review, the nanomaterials for vaccine application are focused intensively in poly(lactic-co-glycolic) acid (PLGA), dendrimers, liposomes, nanogels and micelles which are the targeted antigen delivery system, and present high potential as a promising future strategy for DNA-based, bacterial and viral vaccines. Further advances in nanotechnology and molecular immunology techniques will enhance the success of targeting and lead to the next generation of nano-delivery systems.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68369363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}