聚乙二醇和二磷酸氯喹复合包埋化学合成的金纳米粒子作为一种改进的抗疟药物

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
Shittu Oluwatosin Kudirat, A. Tawakalitu, A. A. Saka, Abubakre O. Kamaldeen, T. BankoleMercy, T. Oladejo
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引用次数: 1

摘要

目的:药物递送是一种控制治疗剂向靶器官、组织和细胞的释放和递送的工程技术。金属纳米颗粒,如金纳米颗粒(AuNPs)具有特殊的性质,能够有效地将药物转运到不同类型的细胞中,减少副作用和对其他组织的细胞毒性。材料与方法:采用Turkevich法,用柠檬酸钠还原四氯金酸(HAuCl4)溶液,合成AuNPs。采用24的析因设计研究了温度、搅拌速度以及柠檬酸盐和金盐的体积对AuNPs合成尺寸的影响。使用紫外-可见光谱和动态光散射(DLS)对所制备的化学AuNPs(CN-AuNPs)进行了表征,DLS与负载二磷酸氯喹的聚乙二醇(PEG)偶联。用透射电子显微镜(TEM)、能谱仪(EDS)、选区电子衍射(SAED)和傅立叶透射红外光谱对后者进行了表征。在疟原虫感染的小鼠身上测试了三种制剂的抗疟活性。此外,通过寄生虫计数对制剂的治疗潜力进行了评估。研究了纳米胶囊的贫血和病理条件下的细胞毒性水平。结果:CN-AuNPs在较低的柠檬酸盐体积下表现出526至529nm的表面等离子体共振吸收,具有较小的颗粒尺寸。具有聚乙二醇(PEG)的CN AuNPs和用二磷酸氯喹包封的CN AuNP的TEM图像显示球形,EDS显示在2.0、2.1和9.9KeV下出现金(Au)。SAED还揭示了AuNPs在性质上是结晶的。与具有相同细胞毒性水平的寄生虫病清除率的CN-AuNPs-PEG相比,体外时间依赖性包封释放显示出时间释放的延长。结论:虽然CN-AuNPs-PEG包埋剂的活性有所提高,但其细胞毒性仍有局限性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Entrapped chemically synthesized gold nanoparticles combined with polyethylene glycol and chloroquine diphosphate as an improved antimalarial drug
Objective(s): Drug delivery is an engineering technology to control the release and delivery of therapeutic agents to target organs, tissues, and cells. Metallic nanoparticles, such as gold nanoparticles (AuNPs) have exceptional properties which enable efficient drug transport into different cell types with reduced side effects and cytotoxicity to other tissues.Materials and Methods: AuNPs were synthesized by adopting the Turkevich method to reduce tetra chloroauric (III) acid (HAuCl4) solution with sodium citrate. A factorial design of 24 was used to investigate the influence of temperature, stirring speed, and the volume of citrate and gold salt on the size of AuNPs synthesis. The produced chemical-AuNPs (CN-AuNPs) were characterized using ultraviolet-visible spectroscopy and dynamic light scattering (DLS) which was conjugated with polyethylene glycol (PEG) loaded with chloroquine diphosphate. The latter were characterized with transmission electron microscopy (TEM), Energy dispersive x-ray spectroscopy (EDS), selected area electron diffraction (SAED) patterns and Fourier transmission infrared spectroscopy. The antimalarial activities of the three formulations were tested on Plasmodium-infected mice. Moreover, the evaluation of curative potentials of the formulations was carried out via parasite counts. The anemic and pathological conditions of nano-encapsulation were investigated for their cytotoxicity level. Results: The CN-AuNPs show surface plasmon resonance absorption ranging from 526 to 529 nm with smaller particle size at the lower citrate volume. The TEM image of CN-AuNPs with polyethylene glycol (PEG) and CN-AuNPs-PEG encapsulated with chloroquine diphosphate revealed spherical shape with EDS showing the appearance of gold (Au) at 2.0, 2.1, and 9.9 KeV. The SAED also revealed that the AuNPs were crystalline in nature. The in vitro time-dependent encapsulation release showed an extension of time release, compared to CN-AuNPs-PEG with parasitemia clearance at the same level of cytotoxicity. Conclusion: Therefore, although improved activity of the CN-AuNPs-PEG encapsulating was achieved but its cytotoxicity still is a limitation.
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
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