二氧化钛纳米粒子在微重力和重力条件下对MDA-MB-231细胞凋亡作用的比较

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
A. Hekmat, M. Rabizadeh, M. Safavi, Z. Hajebrahimi
{"title":"二氧化钛纳米粒子在微重力和重力条件下对MDA-MB-231细胞凋亡作用的比较","authors":"A. Hekmat, M. Rabizadeh, M. Safavi, Z. Hajebrahimi","doi":"10.22038/NMJ.2019.06.0006","DOIUrl":null,"url":null,"abstract":"Objective (s): Gravity could affect some system features and perform directly as an organizing field factor. Recent investigations have examined the titanium dioxide nanoparticles (TiO2 NPs) in biomedical applications, mostly in the cancer treatment field. This study aimed to evaluate the effects of simulated microgravity combined with TiO2 NPs in MDA-MB-231 cells proliferation for the first time. In other words, this study examined the utility of the microgravity environment in nano-therapy. Materials and Methods: The MDA-MB-231 human breast cancer cell line and TiO2 NPs were purchased. The 2D clinostat was applied for the simulation of the microgravity. The morphological studies, MTT cytotoxicity assay, Acridine orange/Ethidium bromide double staining studies and flow cytometry analysis were utilized.Results: The MTT assay, the morphological studies, Acridine orange/Ethidium bromide double staining studies and flow cytometry analysis confirmed the apoptosis-inducing effect of microgravity in combination with TiO2 NPs. The IC50 of simulated microgravity in the presence of TiO2 NPs was determined to be 130 µM. Furthermore, MDA-MB-231 cells exposed to microgravity adopted a different phenotype. Conclusion: Based on our observation, although the relative mechanisms need to be explored further, microgravity can strictly affect the TiO2 NPs effects on MDA-MB-231 cells. The significance of this study lied in the fact that simulating microgravity can be a powerful physical cure for cancer therapy and open new horizons for the studies in the field of biology, biophysics, and medicine.","PeriodicalId":18933,"journal":{"name":"Nanomedicine Journal","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"The comparison of the apoptosis effects of titanium dioxide nanoparticles into MDA-MB-231 cell line in microgravity and gravity conditions\",\"authors\":\"A. Hekmat, M. Rabizadeh, M. Safavi, Z. Hajebrahimi\",\"doi\":\"10.22038/NMJ.2019.06.0006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective (s): Gravity could affect some system features and perform directly as an organizing field factor. Recent investigations have examined the titanium dioxide nanoparticles (TiO2 NPs) in biomedical applications, mostly in the cancer treatment field. This study aimed to evaluate the effects of simulated microgravity combined with TiO2 NPs in MDA-MB-231 cells proliferation for the first time. In other words, this study examined the utility of the microgravity environment in nano-therapy. Materials and Methods: The MDA-MB-231 human breast cancer cell line and TiO2 NPs were purchased. The 2D clinostat was applied for the simulation of the microgravity. The morphological studies, MTT cytotoxicity assay, Acridine orange/Ethidium bromide double staining studies and flow cytometry analysis were utilized.Results: The MTT assay, the morphological studies, Acridine orange/Ethidium bromide double staining studies and flow cytometry analysis confirmed the apoptosis-inducing effect of microgravity in combination with TiO2 NPs. The IC50 of simulated microgravity in the presence of TiO2 NPs was determined to be 130 µM. Furthermore, MDA-MB-231 cells exposed to microgravity adopted a different phenotype. Conclusion: Based on our observation, although the relative mechanisms need to be explored further, microgravity can strictly affect the TiO2 NPs effects on MDA-MB-231 cells. The significance of this study lied in the fact that simulating microgravity can be a powerful physical cure for cancer therapy and open new horizons for the studies in the field of biology, biophysics, and medicine.\",\"PeriodicalId\":18933,\"journal\":{\"name\":\"Nanomedicine Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2019-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nanomedicine Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22038/NMJ.2019.06.0006\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"NANOSCIENCE & NANOTECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanomedicine Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22038/NMJ.2019.06.0006","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NANOSCIENCE & NANOTECHNOLOGY","Score":null,"Total":0}
引用次数: 6

摘要

目标:重力可以影响一些系统特征,并直接作为组织场因素发挥作用。最近的研究检查了二氧化钛纳米颗粒(TiO2 NP)在生物医学应用中的应用,主要是在癌症治疗领域。本研究旨在首次评估模拟微重力与TiO2纳米粒子联合对MDA-MB-231细胞增殖的影响。换句话说,这项研究考察了微重力环境在纳米治疗中的效用。材料与方法:购买MDA-MB-231人癌症细胞系和TiO2纳米粒子。应用二维倾斜仪模拟微重力。采用形态学研究、MTT细胞毒性试验、吖啶橙/溴化乙锭双染色研究和流式细胞术分析。结果:MTT法、形态学研究、吖啶橙/溴化乙锭双染色研究和流式细胞术分析证实了微重力与TiO2纳米粒子联合诱导细胞凋亡的作用。在TiO2 NP存在的情况下,模拟微重力的IC50被确定为130µM。此外,暴露于微重力的MDA-MB-231细胞采用了不同的表型。结论:根据我们的观察,尽管相关机制有待进一步探索,但微重力可以严格影响TiO2 NPs对MDA-MB-231细胞的作用。这项研究的意义在于,模拟微重力可以成为癌症治疗的强大物理疗法,并为生物学、生物物理学和医学领域的研究开辟了新的视野。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The comparison of the apoptosis effects of titanium dioxide nanoparticles into MDA-MB-231 cell line in microgravity and gravity conditions
Objective (s): Gravity could affect some system features and perform directly as an organizing field factor. Recent investigations have examined the titanium dioxide nanoparticles (TiO2 NPs) in biomedical applications, mostly in the cancer treatment field. This study aimed to evaluate the effects of simulated microgravity combined with TiO2 NPs in MDA-MB-231 cells proliferation for the first time. In other words, this study examined the utility of the microgravity environment in nano-therapy. Materials and Methods: The MDA-MB-231 human breast cancer cell line and TiO2 NPs were purchased. The 2D clinostat was applied for the simulation of the microgravity. The morphological studies, MTT cytotoxicity assay, Acridine orange/Ethidium bromide double staining studies and flow cytometry analysis were utilized.Results: The MTT assay, the morphological studies, Acridine orange/Ethidium bromide double staining studies and flow cytometry analysis confirmed the apoptosis-inducing effect of microgravity in combination with TiO2 NPs. The IC50 of simulated microgravity in the presence of TiO2 NPs was determined to be 130 µM. Furthermore, MDA-MB-231 cells exposed to microgravity adopted a different phenotype. Conclusion: Based on our observation, although the relative mechanisms need to be explored further, microgravity can strictly affect the TiO2 NPs effects on MDA-MB-231 cells. The significance of this study lied in the fact that simulating microgravity can be a powerful physical cure for cancer therapy and open new horizons for the studies in the field of biology, biophysics, and medicine.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
发文量
0
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信