Nanotoxicology最新文献

{"title":"Genotoxicity assessment of titanium dioxide nanoparticles using a standard battery of <i>in vivo</i> assays.","authors":"Nana Sun, Xiaopeng Zhang, Chunlai Liang, Haibo Liu, Yuan Zhi, Jin Fang, Huiling Wang, Zhou Yu, Xudong Jia","doi":"10.1080/17435390.2023.2265467","DOIUrl":"10.1080/17435390.2023.2265467","url":null,"abstract":"Abstract As one representative of nanometal oxides, titanium dioxide nanoparticles (TiO2-NPs) have been widely used, particularly in the food industry. The genotoxicity of TiO2-NPs has attracted great attention over the years. This study was undertaken to investigate the chromosome and DNA damage effects of TiO2-NPs (0, 50, 150, and 500 mg/kg BW) using rodent models. After a comprehensive characterization, we conducted a standard battery of in vivo genotoxicity tests, including the chromosomal aberration test (CA), micronucleus (MN) test, and the comet test. The results of all these tests were negative. There were no structural or numerical chromosomal abnormalities in mice bone marrow cells, no increase in the frequency of micronucleated polychromatic erythrocytes in mice bone marrow cells, and no elevation in % tail DNA in rat hepatocytes. This indicated that TiO2-NPs did not cause chromosomal damage or have a direct impact on DNA. These findings suggested that TiO2-NPs did not exhibit genotoxicity and provided valuable data for risk assessment purposes.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"497-510"},"PeriodicalIF":5.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41236956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/17435390.2023.2285561","DOIUrl":"10.1080/17435390.2023.2285561","url":null,"abstract":"","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"i"},"PeriodicalIF":5.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138299582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.1080/17435390.2023.2265766","DOIUrl":"10.1080/17435390.2023.2265766","url":null,"abstract":"","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"545"},"PeriodicalIF":5.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41205640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiparametric <i>in vitro</i> genotoxicity assessment of different variants of amorphous silica nanomaterials in rat alveolar epithelial cells.","authors":"Fátima Brandão, Carla Costa, Maria João Bessa, Vanessa Valdiglesias, Bryan Hellack, Andrea Haase, Sónia Fraga, João Paulo Teixeira","doi":"10.1080/17435390.2023.2265481","DOIUrl":"10.1080/17435390.2023.2265481","url":null,"abstract":"<p><p>The hazard posed to human health by inhaled amorphous silica nanomaterials (aSiO₂ NM) remains uncertain. Herein, we assessed the cyto- and genotoxicity of aSiO₂ NM variants covering different sizes (7, 15, and 40 nm) and surface modifications (unmodified, phosphonate-, amino- and trimethylsilyl-modified) on rat alveolar epithelial (RLE-6TN) cells. Cytotoxicity was evaluated at 24 h after exposure to the aSiO₂ NM variants by the lactate dehydrogenase (LDH) release and WST-1 reduction assays, while genotoxicity was assessed using different endpoints: DNA damage (single- and double-strand breaks [SSB and DSB]) by the comet assay for all aSiO₂ NM variants; cell cycle progression and γ-H2AX levels (DSB) by flow cytometry for those variants that presented higher cytotoxic and DNA damaging potential. The variants with higher surface area demonstrated a higher cytotoxic potential (SiO₂_7, SiO₂_15_Unmod, SiO₂_15_Amino, and SiO₂_15_Phospho). SiO₂_40 was the only variant that induced significant DNA damage on RLE-6TN cells. On the other hand, all tested variants (SiO₂_7, SiO₂_15_Unmod, SiO₂_15_Amino, and SiO₂_40) significantly increased total γ-H2AX levels. At high concentrations (28 µg/cm²), a decrease in G₀/G₁ subpopulation was accompanied by a significant increase in S and G₂/M sub-populations after exposure to all tested materials except for SiO₂_40 which did not affect cell cycle progression. Based on the obtained data, the tested variants can be ranked for its genotoxic DNA damage potential as follows: SiO₂_7 = SiO₂_40 = SiO₂_15_Unmod > SiO₂_15_Amino. Our study supports the usefulness of multiparametric approaches to improve the understanding on NM mechanisms of action and hazard prediction.</p>","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"511-528"},"PeriodicalIF":5.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49679862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on myocardial toxicity induced by lead halide perovskites nanoparticles.","authors":"Rendong He,&nbsp;Xuefeng Ding,&nbsp;Tingjun Zhang,&nbsp;Linqiang Mei,&nbsp;Shuang Zhu,&nbsp;Chengyan Wang,&nbsp;You Liao,&nbsp;Dongmei Wang,&nbsp;Hao Wang,&nbsp;Junsong Guo,&nbsp;Xiaolan Guo,&nbsp;Yan Xing,&nbsp;Zhanjun Gu,&nbsp;Houxiang Hu","doi":"10.1080/17435390.2023.2255269","DOIUrl":"10.1080/17435390.2023.2255269","url":null,"abstract":"<p><p>Lead halide perovskites (LHPs) are outstanding candidates for next-generation optoelectronic materials, with considerable prospects of use and commercial value. However, knowledge about their toxicity is scarce, which may limit their commercialization. Here, for the first time, we studied the cardiotoxicity and molecular mechanisms of representative CsPbBr₃ nanoparticles in LHPs. After their intranasal administration to Institute of Cancer Research (ICR) mice, using advanced synchrotron radiation, mass spectrometry, and ultrasound imaging, we revealed that CsPbBr₃ nanoparticles can severely affect cardiac systolic function by accumulating in the myocardial tissue. RNA sequencing and Western blotting demonstrated that CsPbBr₃ nanoparticles induced excessive oxidative stress in cardiomyocytes, thereby provoking endoplasmic reticulum stress, disturbing calcium homeostasis, and ultimately leading to apoptosis. Our findings highlight the cardiotoxic effects of LHPs and provide crucial toxicological data for the product.</p>","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"449-470"},"PeriodicalIF":5.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10188065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile fabrication of biocompatible injectable blended polymeric hydrogel with bioactive nanoformulation to improving cardiac tissue regeneration efficiency after myocardial infarction for nursing care potential applications.","authors":"Qinqin Sun,&nbsp;Jia Yao,&nbsp;Zhijun Zhang,&nbsp;Juan Li,&nbsp;Xue Zhang,&nbsp;Hui Wang,&nbsp;Xufang Du,&nbsp;Min Li,&nbsp;Ying Zhao","doi":"10.1080/17435390.2023.2252921","DOIUrl":"10.1080/17435390.2023.2252921","url":null,"abstract":"<p><p>Recent years, cardiac vascular disease has arisen owing to acute myocardial infarction (MI) and heart failure leading to death worldwide. Various treatments are available for MI in modern medicine such as implantation of devices, pharmaceutical therapy, and transplantation of organs, nonetheless, it has many complications in finding an organ donor, devices for stenosis, high intrusiveness and long-time hospitalization. To overcome these problems, we have designed and developed a novel hydrogel material with a combination of Se NPs loaded poly(ethylene glycol)/tannic acid (PEG/TA) hydrogel for the treatment of acute MI repair. Herein, Se NPs were characterized by effective analytical and spectroscopic techniques. <i>In vitro</i> cell compatibility and anti-oxidant analyses were examined on human cardiomyocytes in different concentrations of Se NPs and appropriate Se NPs loaded hydrogel samples to demonstrate its greater suitability for <i>in vivo</i> cardiac applications. <i>In vivo</i> investigations of MI mice models injected with Se hydrogels established that LV wall thickness was conserved significantly from the value of 235.6 µm to 390 µm. In addition, the relative scar thickness (33.6%) and infarct size (17.1%) of the MI model were enormously reduced after injection of Se hydrogel when compared to the Se NPs and control (MI) sample, respectively, which confirmed that Se introduced hydrogel have greatly influenced on the restoration of the infarcted heart. Based on the investigated results of the nanoformulation samples, it could be a promising material for future generations treatment of acute myocardial infarction and cardiac repair applications.</p>","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"432-448"},"PeriodicalIF":5.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10303014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-5622-3p inhibits ZCWPW1 to induce apoptosis in silica-exposed mice and spermatocyte cells.","authors":"Moxuan Zhao,&nbsp;Guiqing Zhou,&nbsp;Jingjing Wang,&nbsp;Yue Zhang,&nbsp;Jinglong Xue,&nbsp;Jianhui Liu,&nbsp;Junhong Xie,&nbsp;Lihua Ren,&nbsp;Xianqing Zhou","doi":"10.1080/17435390.2023.2223632","DOIUrl":"https://doi.org/10.1080/17435390.2023.2223632","url":null,"abstract":"<p><p>Silica nanoparticles (SiNPs) could cause damage to spermatogenesis, and microRNAs were reported to be associated with male reproduction. This research was designed to explore the toxic impacts of SiNPs induced in male reproduction through miR-5622-3p. In vivo, 60 mice were randomized into the control group and SiNPs group, in which they were exposed to SiNPs for 35 days and then recovered for 15 days. In vitro, 4 groups were set: control group, SiNPs group, SiNPs + miR-5622-3p inhibitor group, and SiNPs + miR-5622-3p inhibitor negative control (NC) group. Our research indicated SiNPs caused the apoptosis of spermatogenic cells, increased level of γ-H2AX, raised the expressions of RAD51, DMC1, 53BP1, and LC8 which were DNA damage repair relative factors, and upregulated Cleaved-Caspase-9 and Cleaved-Caspase-3 levels. Furthermore, SiNPs also elevated the expression of miR-5622-3p but downregulated the level of ZCWPW1. However, miR-5622-3p inhibitor reduced the level of miR-5622-3p, increased the level of ZCWPW1, relieved DNA damage, and depressed the activation of apoptosis pathway, thus, alleviating spermatogenic cells apoptosis caused by SiNPs. The above-mentioned results indicated that SiNPs induced DNA damage resulting in activating of DNA damage response. Meanwhile, SiNPs raised the level of miR-5622-3p targeting inhibited expression of ZCWPW1 to suppress the repair process, possibly making DNA damage so severe that leading to the failure of DNA damage repair, finally inducing the apoptosis of spermatogenic cells.</p>","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":"17 4","pages":"372-384"},"PeriodicalIF":5.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9800238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of natural biomolecules on yttrium oxide nanoparticles from a <i>Daphnia magna</i> survival rate perspective.","authors":"Egle Kelpsiene,&nbsp;Tingru Chang,&nbsp;Aliaksandr Khort,&nbsp;Katja Bernfur,&nbsp;Inger Odnevall,&nbsp;Tommy Cedervall,&nbsp;Jing Hua","doi":"10.1080/17435390.2023.2226712","DOIUrl":"https://doi.org/10.1080/17435390.2023.2226712","url":null,"abstract":"<p><p>The attention to rare earth oxide nanoparticles (NPs), including yttrium oxide (Y₂O₃), has increased in many fields due to their unique structural characteristics and functional properties. The aim of our study was to investigate the mechanisms by which bio-corona formation on Y₂O₃ NPs affects their environmental fate and toxicity. The Y₂O₃ NPs induced toxicity to freshwater filter feeder <i>Daphnia magna</i> at particle concentrations of 1 and 10 mg/L, regardless of particle size. Interactions between naturally excreted biomolecules (e.g. protein, lipids, and polysaccharides) derived from <i>D. magna,</i> and the Y₂O₃ NPs (30-45 nm) resulted in the formation of an eco-corona, which reduced their toxic effects toward <i>D. magna</i> at a particle concentration of 10 mg/L. No effects were observed at lower concentrations or for the other particle sizes investigated. Copper-zinc (Cu-Zn) superoxide dismutase, apolipophorins, and vitellogenin-1 proteins proved to be the most prominent proteins of the adsorbed corona, and possibly a reason for the reduced toxicity of the 30-45 nm Y₂O₃ NPs toward <i>D. magna.</i></p>","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":"17 4","pages":"385-399"},"PeriodicalIF":5.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10169792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harmonizing nanomaterial exposure methodologies in ecotoxicology: the effects of two innovative nanoclays in the freshwater microalgae <i>Raphidocelis subcapitata</i>.","authors":"Fábio Campos,&nbsp;Patrícia V Silva,&nbsp;Amadeu M V M Soares,&nbsp;Roberto Martins,&nbsp;Susana Loureiro","doi":"10.1080/17435390.2023.2231071","DOIUrl":"10.1080/17435390.2023.2231071","url":null,"abstract":"<p><p>Layered double hydroxides (LDHs) are innovative nanomaterials (NMs) with a typical nanoclay structure (height <40 nm) consisting of layers of metallic cations and hydroxides stabilized by anions and water molecules. Upon specific triggers, anions can exchange by others in the surrounding environment. Due to this stimuli-responsive behavior, LDHs are used as carriers of active ingredients in the industrial or pharmaceutical sectors. Available technical guidelines to evaluate the ecotoxicity of conventional substances do not account for the specificities of NMs, leading to inaccuracies and uncertainty. The present study aimed to assess two different exposure methodologies (serial dilutions of the stock dispersion vs. direct addition of NM powder to each concentration) on the ecotoxicological profile of different powder grain sizes of Zn-Al LDH-NO₃ and Cu-Al LDH-NO₃ (bulk, <25, 25-63, 63-125, 125-250, and >250 µm) in the growth of the freshwater microalgae <i>Raphidocelis subcapitata</i>. Results revealed that the serial dilutions methodology was preferable for Zn-Al LDH-NO₃, whereas for Cu-Al LDH-NO₃ both methodologies were suitable. Thus, the serial dilutions methodology was selected to assess the ecotoxicity of different grain sizes for both LDHs. All Zn-Al LDH-NO₃ grain sizes yielded similar toxicity, while Cu-Al LDH-NO₃ powders with smaller grain sizes caused a higher effect on microalgae growth; thus, grain size separation might be advantageous for future applications of Cu-Al LDH-NO₃s. Considering the differences between exposure methodologies for the Zn-Al LDH-NO₃, further research involving other NMs and species must be carried out to achieve harmonization and validation for inter-laboratory comparison.</p>","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"401-419"},"PeriodicalIF":5.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9878847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of cell membrane-based biomimetic nanoparticles loaded by <i>Clostridium perfringens</i> epsilon toxin: a novel vaccine delivery platform for <i>Clostridial</i>-associated diseases.","authors":"Mokarameh Pudineh Moarref,&nbsp;Mojtaba Alimolaei,&nbsp;Tara Emami,&nbsp;Mohammad Kazem Koohi","doi":"10.1080/17435390.2023.2252899","DOIUrl":"10.1080/17435390.2023.2252899","url":null,"abstract":"<p><p>As <i>Clostridium perfringens</i> (<i>C. perfringens</i>) epsilon toxin (ETX) ranks as the third most potent clostridial toxin after botulinum and tetanus toxins, vaccination is necessary for creatures that can be affected by it to be safe from the effects of this toxin. Nowadays, nanostructures are good choices for carriers for biological environments. We aimed to synthesize biomimetic biodegradable nanodevices to enhance the efficiency of the ETX vaccine. For this purpose, poly(lactic-co-glycolic acid) (PLGA) copolymer loaded with purified epsilon protoxin (proETX) to create nanoparticles called nanotoxins (NTs) and then coated by RBC membrane-derived vesicles (RVs) to form epsilon nanotoxoids (RV-NTs). The resulting RV-NTs shaped smooth spherical surfaces with double-layer core/shell structure with an average particle size of 105.9 ± 35.1 nm and encapsulation efficiency of 97.5% ± 0.13%. Compared with NTs, the RV-NTs were more stable for 15 consecutive days. In addition, although both structures showed a long-term cumulative release, the release rates from RV-NTs were slower than NTs during 144 hours. According to the results of cell viability, ETX loading in PLGA and entrapment in the RBC membrane decreased the toxicity of the toxin. The presence of PLGA enhances the uptake of proETX, and the synthesized structures showed no significant lesion after injection. These results demonstrate that NTs and RV-NTs could serve as an effective vaccine platform to deliver ETX for future <i>in vivo</i> assays.</p>","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"420-431"},"PeriodicalIF":5.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10206476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}