Nanotoxicology最新文献_第7页

Nano-graphene oxide particles induce inheritable anomalies through altered gene expressions involved in oocyte maturation. 纳米氧化石墨烯颗粒通过改变参与卵母细胞成熟的基因表达诱发遗传异常。

IF 5 3区医学

Nanotoxicology Pub Date : 2024-03-01 Epub Date: 2024-03-07 DOI: 10.1080/17435390.2024.2325615

Sreelakshmi Krishnakumar, Raghunath Nair Malavika, Shantikumar V Nair, Deepthy Menon, Bindhu Paul-Prasanth

{"title":"Nano-graphene oxide particles induce inheritable anomalies through altered gene expressions involved in oocyte maturation.","authors":"Sreelakshmi Krishnakumar, Raghunath Nair Malavika, Shantikumar V Nair, Deepthy Menon, Bindhu Paul-Prasanth","doi":"10.1080/17435390.2024.2325615","DOIUrl":"10.1080/17435390.2024.2325615","url":null,"abstract":"The inheritable impact of exposure to graphene oxide nanoparticles (GO NPs) on vertebrate germline during critical windows of gamete development remain undetermined to date. Here, we analyzed the transgenerational effects of exposure to nano-graphene oxide particles (nGO) synthesized in house with lateral dimensions 300-600 nm and surface charge of -36.8 mV on different developmental stages of germ cells (GCs): (1) during GCs undergoing early development and differentiation, and (2) during GCs undergoing gametogenesis and maturation in adulthood. Biocompatibility analyses in Japanese medaka embryos showed lethality above 1 µg/ml and also an aberrant increase in germ cell count of both males and females at doses below the lethal dose. However, no lethality or anomalies were evident in adults up to 45 µg/ml. Long term exposure of embryos and adults for 21 days resulted in reduced fecundity. This effect was transmitted to subsequent generations, F1 and F2. Importantly, the inheritable effects of nGO in adults were pronounced at a high dose of 10 µg/ml, while 1 µg/ml showed no impact on the germline indicating lower doses used in this study to be safe. Further, expressions of selected genes that adversely affected oocyte maturation were enhanced in F1 and F2 individuals. Interestingly, the inheritance patterns differed corresponding to the stage at which the fish received the exposure.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"160-180"},"PeriodicalIF":5.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140049939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of carbon nanostructures in biomedicine: realities, difficulties, prospects. 碳纳米结构在生物医学中的应用：现实、困难和前景。

IF 5 3区医学

Nanotoxicology Pub Date : 2024-03-01 Epub Date: 2024-03-15 DOI: 10.1080/17435390.2024.2327053

Konstantin N Semenov, Sergei V Ageev, Olegi N Kukaliia, Igor V Murin, Andrey V Petrov, Gleb O Iurev, Pavel A Andoskin, Gaiane G Panova, Oleg E Molchanov, Dmitrii N Maistrenko, Vladimir V Sharoyko

引用次数: 0

Analysis of carbon nanotube levels in organic matter: an inter-laboratory comparison to determine best practice. 有机物质中碳纳米管含量的分析：确定最佳做法的实验室间比较。

IF 5 3区医学

Nanotoxicology Pub Date : 2024-03-01 Epub Date: 2024-04-01 DOI: 10.1080/17435390.2024.2331683

Jérôme Devoy, Souhail Al-Abed, Benjamin Cerdan, Wan-Seob Cho, David Dubuc, Emmanuel Flahaut, Katia Grenier, Stéphane Grossmann, Mary Gulumian, Jiyoung Jeong, Boo Wook Kim, Adam Laycock, Jong Seong Lee, Rachel Smith, Mei Yang, Il Je Yu, Minfang Zhang, Frédéric Cosnier

{"title":"Analysis of carbon nanotube levels in organic matter: an inter-laboratory comparison to determine best practice.","authors":"Jérôme Devoy, Souhail Al-Abed, Benjamin Cerdan, Wan-Seob Cho, David Dubuc, Emmanuel Flahaut, Katia Grenier, Stéphane Grossmann, Mary Gulumian, Jiyoung Jeong, Boo Wook Kim, Adam Laycock, Jong Seong Lee, Rachel Smith, Mei Yang, Il Je Yu, Minfang Zhang, Frédéric Cosnier","doi":"10.1080/17435390.2024.2331683","DOIUrl":"10.1080/17435390.2024.2331683","url":null,"abstract":"Carbon nanotubes (CNTs) are increasingly being used in industrial applications, but their toxicological data in animals and humans are still sparse. To assess the toxicological dose-response of CNTs and to evaluate their pulmonary biopersistence, their quantification in tissues, especially lungs, is crucial. There are currently no reference methods or reference materials for low levels of CNTs in organic matter. Among existing analytical methods, few have been fully and properly validated. To remedy this, we undertook an inter-laboratory comparison on samples of freeze-dried pig lung, ground and doped with CNTs. Eight laboratories were enrolled to analyze 3 types of CNTs at 2 concentration levels each in this organic matrix. Associated with the different analysis techniques used (specific to each laboratory), sample preparation may or may not have involved prior digestion of the matrix, depending on the analysis technique and the material being analyzed. Overall, even challenging, laboratories' ability to quantify CNT levels in organic matter is demonstrated. However, CNT quantification is often overestimated. Trueness analysis identified effective methods, but systematic errors persisted for some. Choosing the assigned value proved complex. Indirect analysis methods, despite added steps, outperform direct methods. The study emphasizes the need for reference materials, enhanced precision, and organized comparisons.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"214-228"},"PeriodicalIF":5.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140336175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress in the EU classification of the health hazards associated with certain multiwall carbon nanotubes (MWCNTs): what about the other MWCNTs? 欧盟对与某些多壁碳纳米管（MWCNTs）相关的健康危害进行分类的最新进展：其他的多壁碳纳米管呢？

IF 3.6 3区医学

Nanotoxicology Pub Date : 2024-03-01 Epub Date: 2024-02-27 DOI: 10.1080/17435390.2024.2321885

Henrik Wolff, Ulla Vogel

引用次数: 0

Genoprotective role of pembrolizumab liposome in isolated lymphocytes from head and neck squamous cell carcinoma patients compared to those from healthy individuals in vitro. 与健康人的离体淋巴细胞相比，pembrolizumab 脂质体在头颈部鳞状细胞癌患者离体淋巴细胞中的基因保护作用。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2024-02-01 Epub Date: 2024-03-07 DOI: 10.1080/17435390.2024.2314464

Nagah Bobtina, Maysa Alhawamdeh, Khaled Habas, Mohamed Isreb, Bayan Aburas, Andrew T Harris, Mojgan Najafzadeh, Diana Anderson

{"title":"Genoprotective role of pembrolizumab liposome in isolated lymphocytes from head and neck squamous cell carcinoma patients compared to those from healthy individuals in vitro.","authors":"Nagah Bobtina, Maysa Alhawamdeh, Khaled Habas, Mohamed Isreb, Bayan Aburas, Andrew T Harris, Mojgan Najafzadeh, Diana Anderson","doi":"10.1080/17435390.2024.2314464","DOIUrl":"10.1080/17435390.2024.2314464","url":null,"abstract":"Pembrolizumab has shown significant anticancer effects against various human cancers. The present study investigated the effects of pembrolizumab liposome and nano (naked) forms in treated lymphocytes from head and neck squamous cell carcinoma (HNSCC) patients compared to healthy individuals. The level of oxidative DNA damage induced by hydrogen peroxide (H2O2) was also investigated. A concentration of 10 µg/ml of pembrolizumab liposome was used to treat the lymphocytes in the Comet and micronucleus assays based on the preliminary dosage optimization tests. To determine the cellular pathways involved in the protective role of pembrolizumab against H2O2, several proteins involved in apoptosis (P53, P21 and Bcl-2) were assessed. Pembrolizumab significantly reduced DNA damage and decreased the number of micronuclei in lymphocytes from HNSCC patients (p < 0.01) compared with healthy individuals. The 10 µg/ml of pembrolizumab liposome significantly reduced the oxidative stress induced by H2O2 and was effective in healthy and HNSCC groups using the Comet and micronucleus assays (p < 0.001). To our knowledge, this is the first report of pembrolizumab in liposome and naked forms exhibiting a protective effect on DNA damage in the treatment of HNSCC patients.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"55-68"},"PeriodicalIF":3.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140049938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of acute and subacute toxicity, pharmacokinetics, and biodistribution of eugenol nanoparticles after oral exposure in Wistar rats. 评估 Wistar 大鼠口服丁香酚纳米颗粒后的急性和亚急性毒性、药代动力学和生物分布。

IF 5 3区医学

Nanotoxicology Pub Date : 2024-02-01 Epub Date: 2024-02-13 DOI: 10.1080/17435390.2024.2314483

Pramod G Nagaraju, Ashwini S, Pooja J Rao, Poornima Priyadarshini

{"title":"Assessment of acute and subacute toxicity, pharmacokinetics, and biodistribution of eugenol nanoparticles after oral exposure in Wistar rats.","authors":"Pramod G Nagaraju, Ashwini S, Pooja J Rao, Poornima Priyadarshini","doi":"10.1080/17435390.2024.2314483","DOIUrl":"10.1080/17435390.2024.2314483","url":null,"abstract":"The present study aimed to assess the safety, toxicity, biodistribution, and pharmacokinetics of eugenol nanoparticles (EONs) following oral administration in Wistar rat models. In the acute toxicity study, the rats were given a fixed dose of 50, 300, and 2000 mg/kg body weight per group orally and screened for 2 weeks after administration. In the subacute study, three different doses (500, 1000, and 2000 mg/kg BW) of EON were administered for 28 days. The results indicated no significant differences in food and water consumption, bodyweight change, hematological and biochemical parameters, relative organ weights, gross findings, or histopathology compared to the control. Additionally, no significant changes were observed in the expression profiles of inflammatory cytokines such as IL-1, IL-6, and TNFα in the plasma, confirming the absence of systemic inflammation. Biodistribution analysis revealed rapid absorption of eugenol and improved bioavailability due to gradual and sustained release, leading to a maximum eugenol concentration of 15.05 μg/mL (Cmax) at approximately 8 h (Tmax) in the blood plasma. Thus, the study provides valuable insights into the utilization of EON for enhancing the stability, solubility, and sustained release of eugenol and highlights its promising safety profile in vivo.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"87-105"},"PeriodicalIF":5.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139723351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomarkers of exposure and effect in human biomonitoring of metal-based nanomaterials: their use in primary prevention and health surveillance. 金属基纳米材料人体生物监测中的暴露和影响生物标志物：在初级预防和健康监测中的应用。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2024-02-01 Epub Date: 2024-03-04 DOI: 10.1080/17435390.2023.2301692

Beatrice Bocca, Beatrice Battistini

{"title":"Biomarkers of exposure and effect in human biomonitoring of metal-based nanomaterials: their use in primary prevention and health surveillance.","authors":"Beatrice Bocca, Beatrice Battistini","doi":"10.1080/17435390.2023.2301692","DOIUrl":"10.1080/17435390.2023.2301692","url":null,"abstract":"Metal-based nanomaterials (MNMs) have gained particular interest in nanotechnology industry. They are used in various industrial processes, in biomedical applications or to improve functional properties of several consumer products. The widescale use of MNMs in the global consumer market has resulted in increases in the likelihood of exposure and risks to human beings. Human exposure to MNMs and assessment of their potential health effects through the concomitant application of biomarkers of exposure and effect of the most commonly used MNMs were reviewed in this paper. In particular, interactions of MNMs with biological systems and the nanobiomonitoring as a prevention tool to detect the early damage caused by MNMs as well as related topics like the influence of some physicochemical features of MNMs and availability of analytical approaches for MNMs testing in human samples were summarized in this review. The studies collected and discussed seek to increase the current knowledge on the internal dose exposure and health effects of MNMs, highlighting the advantages in using biomarkers in primary prevention and health surveillance.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"1-35"},"PeriodicalIF":3.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140022215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cationic solid lipid nanoparticles (SLN) complexed with plasmid DNA enhance prostate cancer cells (PC-3) migration. 与质粒 DNA 复合物的阳离子固体脂质纳米颗粒（SLN）可增强前列腺癌细胞（PC-3）的迁移能力。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2024-02-01 DOI: 10.1080/17435390.2024.2307616

Fernanda Garcia-Fossa, Marcelo Bispo de Jesus

{"title":"Cationic solid lipid nanoparticles (SLN) complexed with plasmid DNA enhance prostate cancer cells (PC-3) migration.","authors":"Fernanda Garcia-Fossa, Marcelo Bispo de Jesus","doi":"10.1080/17435390.2024.2307616","DOIUrl":"10.1080/17435390.2024.2307616","url":null,"abstract":"Nanotechnology applications in biomedicine have increased in recent decades, primarily as therapeutic agents, drugs, and gene delivery systems. Among the nanoparticles used in medicine, we highlight cationic solid lipid nanoparticles (SLN). Given their nontoxic properties, much research has focused on the beneficial effects of SLN for drug or gene delivery system. However, little attention has been paid to the adverse impacts of SLN on the cellular environment, particularly their influence on intracellular signaling pathways. In this work, we investigate the effects triggered by cationic SLN on human prostate non-tumor cells (PNT1A) and tumor cells (PC-3). Our results demonstrate that cationic SLN enhances the migration of PC-3 prostate cancer cells but not PNT1A non-tumor prostate cells, an unexpected and unprecedented development. Furthermore, we observed that the enhanced cell migration velocity is a concentration-dependent and nanoparticle-dependent effect, and not related to any individual nanoparticle component. Moreover, cationic SLN increased vimentin expression (p < 0.05) but SLN did not affect Smad2 nuclear translocation. Meanwhile, EMT-related (epithelial-to-mesenchymal transition) proteins, such as ZEB1, underwent nuclear translocation when treated with cationic SLN, thereby affecting PC-3 cell motility through ZEB1 and vimentin modulation. From a therapeutic perspective, cationic SLN could potentially worsen a patient's condition if these results were reproduced in vivo. Understanding the in vitro molecular mechanisms triggered by nanomaterials and their implications for cell function is crucial for defining their safe and effective use.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"36-54"},"PeriodicalIF":3.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139651152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potent lung tumor promotion by inhaled MWCNT. 吸入 MWCNT 可有效促进肺部肿瘤生长。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2024-02-01 Epub Date: 2024-02-29 DOI: 10.1080/17435390.2024.2314473

Dale W Porter, Marlene S Orandle, Ann Hubbs, Lauren M Staska, David Lowry, Michael Kashon, Michael G Wolfarth, Walter McKinney, Linda M Sargent

{"title":"Potent lung tumor promotion by inhaled MWCNT.","authors":"Dale W Porter, Marlene S Orandle, Ann Hubbs, Lauren M Staska, David Lowry, Michael Kashon, Michael G Wolfarth, Walter McKinney, Linda M Sargent","doi":"10.1080/17435390.2024.2314473","DOIUrl":"10.1080/17435390.2024.2314473","url":null,"abstract":"In the lung, carcinogenesis is a multi-stage process that includes initiation by a genotoxic agent, promotion that expands the population of cells with damaged DNA to form a tumor, and progression from benign to malignant neoplasms. We have previously shown that Mitsui-7, a long and rigid multi-walled carbon nanotube (MWCNT), promotes pulmonary carcinogenesis in a mouse model. To investigate the potential exposure threshold and dose-response for tumor promotion by this MWCNT, 3-methylcholanthrene (MC) initiated (10 μg/g, i.p., once) or vehicle (corn oil) treated B6C3F1 mice were exposed by inhalation to filtered air or MWCNT (5 mg/m3) for 5 h/day for 0, 2, 5, or 10 days and were followed for 17 months post-exposure for evidence of lung tumors. Pulmonary neoplasia incidence in MC-initiated mice significantly increased with each MWCNT exposure duration. Exposure to either MC or MWCNT alone did not affect pulmonary neoplasia incidence compared with vehicle controls. Lung tumor multiplicity in MC-initiated mice also significantly increased with each MWCNT exposure duration. Thus, a significantly higher lung tumor multiplicity was observed after a 10-day MWCNT exposure than following a 2-day exposure. Both bronchioloalveolar adenoma and bronchioloalveolar adenocarcinoma multiplicity in MC-initiated mice were significantly increased following 5- and 10-day MWCNT exposure, while a 2-day MWCNT exposure in MC-initiated mice significantly increased the multiplicity of adenomas but not adenocarcinomas. In this study, even the lowest MWCNT exposure promoted lung tumors in MC-initiated mice. Our findings indicate that exposure to this MWCNT strongly promotes pulmonary carcinogenesis.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"69-86"},"PeriodicalIF":3.6,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11057902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139990672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicity study of pro-angiogenic casein manganese oxide nanoparticles: an in vitro and in vivo approach 促血管生成酪蛋白氧化锰纳米颗粒的毒性研究：一种体外和体内方法

IF 5 3区医学

Nanotoxicology Pub Date : 2023-12-18 DOI: 10.1080/17435390.2023.2291788

Shagufta Haque, Sanchita Tripathy, Yogesh Chandra, Kathirvel Muralidharan, Chitta Ranjan Patra

引用次数: 0