Nanotoxicology最新文献

Decoding silica nanoparticle toxicity: integrating machine learning, feature importance, rule extraction, and adsorption-uptake processes. 解码二氧化硅纳米颗粒毒性：整合机器学习，特征重要性，规则提取和吸附-摄取过程。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2026-09-01 Epub Date: 2026-06-25 DOI: 10.1080/17435390.2026.2684422

Roni Romano, Alexander Barbul, Rafi Korenstein

{"title":"Decoding silica nanoparticle toxicity: integrating machine learning, feature importance, rule extraction, and adsorption-uptake processes.","authors":"Roni Romano, Alexander Barbul, Rafi Korenstein","doi":"10.1080/17435390.2026.2684422","DOIUrl":"10.1080/17435390.2026.2684422","url":null,"abstract":"The complexity of nanoparticle toxicity necessitates applying machine learning to large toxicological datasets to identify predictive features and toxicity rules. This study investigates the relationships between the physicochemical properties of silica nanoparticles (SiNPs), external experimental parameters, and toxicity under in vitro conditions. Data from the literature, databases, and in-house experiments were balanced using our Balanced Fitted Dose-Response approach, yielding nearly equal numbers of concentration data points across toxicity levels. The CatBoost algorithm outperformed Decision Tree models in predicting SiNP toxicity. The Catboost Shapley value analysis revealed the following sequence of feature importance: Mass > Total Surface Area > Serum > Total NP Number ∼ Primary Size > Exposure Time > Chemical Surface Modification > Cell Age > Cell Culture > Cell Disease. Rule extraction from a transparent decision-tree model revealed detailed information on the interrelationships among the features of surface-modified SiNPs and their toxicity. It showed that surface-unmodified SiNPs-OH are more toxic than surface-modified SiNPs-NH2 and SiNPs-COOH. Adsorption and uptake measurements by imaging flow cytometry, employing alveolar macrophages, revealed significantly higher adsorption for SiNPs-OH compared with modified SiNPs, while uptake of SiNPs-OH was lower. The integration of computational and experimental findings suggests that adsorption of SiNPs to the macrophage membrane contributes to their toxicity, in addition to internalization, providing valuable insight into the mechanisms underlying nanoparticle safety.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"279-296"},"PeriodicalIF":3.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148331392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benefits, risks, and future prospectives of nanomaterials in sustainable agriculture: a review. 纳米材料在可持续农业中的效益、风险及未来展望

IF 3.6 3区医学

Nanotoxicology Pub Date : 2026-09-01 Epub Date: 2026-06-26 DOI: 10.1080/17435390.2026.2692088

Niharika Pandey, Diksha Sharma, Aayeisha Das, Toran Singh, Akansha Pant, Sneh Gautam, Pushpa Lohani

{"title":"Benefits, risks, and future prospectives of nanomaterials in sustainable agriculture: a review.","authors":"Niharika Pandey, Diksha Sharma, Aayeisha Das, Toran Singh, Akansha Pant, Sneh Gautam, Pushpa Lohani","doi":"10.1080/17435390.2026.2692088","DOIUrl":"10.1080/17435390.2026.2692088","url":null,"abstract":"Modern agriculture is under increasing pressure to meet the food demands of a growing global population while reducing the environmental and health impacts associated with intensive chemical inputs. This study aims to evaluate the role of nanotechnology in promoting sustainable agricultural practices, with a focus on its applications in crop nutrition, crop protection, soil and water management, and post-harvest preservation. The review highlights that nano-fertilizers, particularly those containing essential nutrients such as zinc, phosphorus, and nitrogen, significantly improve nutrient use efficiency, enhance photosynthetic performance, and increase plant resilience to abiotic stress compared to conventional fertilizers. Similarly, nano-pesticides and nano-herbicides enable targeted and controlled delivery of active compounds, resulting in reduced agrochemical usage and minimized adverse effects on non-target organisms and ecosystems. In addition, nanomaterials contribute to soil health by improving water retention, nutrient availability, and overall fertility, while their application in water purification enhances irrigation quality. Post-harvest technologies, including antimicrobial nano-coatings and smart packaging systems, have also been shown to effectively extend shelf life and reduce food losses along the supply chain. These findings suggest that nanotechnology offers a promising pathway toward improving agricultural efficiency while addressing environmental sustainability concerns. However, further research is needed to assess long-term ecological impacts, scalability, and regulatory frameworks. Overall, the integration of nanotechnology into agricultural systems has the potential to support resilient, resource-efficient, and sustainable food production for the future.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"310-330"},"PeriodicalIF":3.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148339457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polystyrene nanoparticles and death of erythrocytes: does exposure induce eryptosis? 聚苯乙烯纳米颗粒与红细胞死亡：接触是否会诱发红血病？

IF 3.6 3区医学

Nanotoxicology Pub Date : 2026-09-01 Epub Date: 2026-06-13 DOI: 10.1080/17435390.2026.2685741

Kamil Płuciennik, Bożena Bukowska, Paulina Sicińska

{"title":"Polystyrene nanoparticles and death of erythrocytes: does exposure induce eryptosis?","authors":"Kamil Płuciennik, Bożena Bukowska, Paulina Sicińska","doi":"10.1080/17435390.2026.2685741","DOIUrl":"10.1080/17435390.2026.2685741","url":null,"abstract":"The widespread presence of polystyrene nanoparticles (PS-NPs) in the human body has raised concerns about their potential biological toxicity. When assessing the safety of nanoparticles and other xenobiotics, a key aspect is their effect on blood cells, particularly erythrocytes, which experience the most direct exposure to nanoparticles circulating in the bloodstream. The present study evaluated the impact of non-functionalized PS-NPs with diameters of ∼30 nm, ∼45 nm, and ∼70 nm on human red blood cells after 24 hours of incubation. The studied PS-NPs did not influence intracellular Ca2+ ion levels or caspase 3 activity, and did not induce phosphatidylserine translocation at pre-hemolytic concentrations (below 100 µg/mL). Moreover, exposure did not increase intracellular ROS levels at any of the tested concentrations, suggesting that generalized intracellular oxidative stress is likely not the primary mechanism underlying the effects induced by PS-NPs in human erythrocytes. However, the observed increase in lipid peroxidation, detected from 50 µg/ml, indicates that oxidative damage at the membrane level may contribute to the overall toxic response. The results suggest that PS-NPs interact primarily with the erythrocyte membrane, leading to membrane destabilization associated with increased calpain activity, observed already at a concentration of 1 µg/mL, and enhanced lipid peroxidation. It is noteworthy that the most pronounced changes were induced by the smallest nanoparticles (∼30 nm), suggesting a size-dependent effect on erythrocyte integrity. These findings suggest that PS-NP toxicity in erythrocytes is mediated mainly through red blood cell membrane destabilization rather than classical intracellular oxidative stress alone.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"297-309"},"PeriodicalIF":3.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of soil microbial activity and Basil growth by graphene oxide nanoparticles: insights from a growth chamber experiment. 氧化石墨烯纳米颗粒增强土壤微生物活性和罗勒生长：来自生长室实验的见解。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2026-08-24 DOI: 10.1080/17435390.2026.2722352

Maryam Rahmati, Ali Ashraf Soltani Toularoud, Tohid Rouhi-Kelarlou, Esmaiel Goli Kalanpa, Mahnaz Alizadeh

{"title":"Enhancement of soil microbial activity and Basil growth by graphene oxide nanoparticles: insights from a growth chamber experiment.","authors":"Maryam Rahmati, Ali Ashraf Soltani Toularoud, Tohid Rouhi-Kelarlou, Esmaiel Goli Kalanpa, Mahnaz Alizadeh","doi":"10.1080/17435390.2026.2722352","DOIUrl":"https://doi.org/10.1080/17435390.2026.2722352","url":null,"abstract":"The extensive application of graphene oxide nanoparticles (GONPs) across various fields, owing to their unique physicochemical properties, has led to their increasing release into soil ecosystems, raising concerns about potential interactions with soil microorganisms and plants. This study investigated the effects of different GONPs concentrations-0 (control), 200, 300, and 600 mg kg-1 soil-on soil microbial indices after 30, 60, and 90 days of exposure, as well as on the growth performance of basil (Ocimum basilicum L.) under controlled growth chamber conditions (temperature: 25 ± 2 °C; photoperiod: 16/8 h light/dark; relative humidity: 60 ± 5%). Increasing GONPs concentrations and longer incubation periods significantly enhanced all microbial parameters (p < 0.01). At 600 mg kg-1 after 90 days of incubation, basal respiration (BR) and substrate-induced respiration (SIR) increased by 3.3- and 2.2-fold, respectively, while the microbial population rose 48-fold, microbial biomass carbon (MBC) increased by 60%, and dehydrogenase activity (DHA) exhibited nearly a sevenfold enhancement compared to the GONPs-free soil. Likewise, basil growth was markedly promoted, with shoot and root dry weights increasing by 2.8- and 7.9-fold, respectively, and root volume by 4.5-fold. The root-to-shoot ratio, leaf area, and plant height increased by 88%, 78% and 39%, respectively. Chlorophyll a, b, and carotenoid contents also rose by 69%, 96% and 3.1-fold. Overall, GONPs application-particularly at 600 mg kg-1-significantly enhanced soil biological activity and basil productivity, highlighting their potential as a nanomaterial for improving soil health, boosting plant growth, and promoting sustainable agriculture.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"1-10"},"PeriodicalIF":3.6,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148808894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding shape- and type-dependent cellular toxicity of plasmonic nanoparticles in healthy and cancer cells. 解码形状和类型依赖的等离子体纳米颗粒在健康细胞和癌细胞中的细胞毒性。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2026-08-11 DOI: 10.1080/17435390.2026.2715136

Şevval Çelikten, Jülide Secerli, Onur Erdem, Hakan Erdoğan, Merve Güdül Bacanlı

{"title":"Decoding shape- and type-dependent cellular toxicity of plasmonic nanoparticles in healthy and cancer cells.","authors":"Şevval Çelikten, Jülide Secerli, Onur Erdem, Hakan Erdoğan, Merve Güdül Bacanlı","doi":"10.1080/17435390.2026.2715136","DOIUrl":"https://doi.org/10.1080/17435390.2026.2715136","url":null,"abstract":"Plasmonic photothermal therapy (PTT) offers a promising route for selective cancer treatment through laser-activated metallic nanoparticles (NPs). Here, we systematically evaluated how NP geometry, size, and elemental composition govern photothermal toxicity responses in healthy fibroblasts (L929) and lung carcinoma cells (A549) under both laser-free and 808 nm laser-irradiated conditions. Spherical, rod-shaped, and star-shaped gold nanoparticles (AuNP, AuNR, AuNS), together with spherical and prismatic silver nanoparticles (AgNP, AgNPr), were synthesized, characterized, and assessed across multiple biological endpoints. Cellular viability (MTT), reactive oxygen species (ROS) generation, DNA damage (Comet assay), inflammatory cytokine release (TNF-α, IL-6, IL-10), and apoptosis (Annexin V/PI staining and caspase-3/9 activation) were systematically analyzed. Laser activation markedly amplified cancer-selective cytotoxicity, with AuNR and AgNPr exhibiting the strongest photothermal responses while largely sparing healthy cells. AuNR and 20 nm AuNP induced pronounced ROS generation and caspase-dependent apoptosis in A549 cells upon irradiation, whereas AgNPr preferentially promoted DNA damage and inflammatory signaling in cancer cells with minimal effects in L929 cells. Across all nanostructures, laser irradiation selectively intensified genotoxic and inflammatory responses in tumor cells, highlighting a strong dependence on nanoparticle geometry and plasmonic resonance matching. Collectively, these results demonstrate that NP shape and photothermal activation synergistically dictate therapeutic/toxic effects and selectivity. AuNR and AgNPr emerge as leading candidates for precision PTT, combining potent anticancer activity with favorable biocompatibility. This work provides mechanistic insight into structure-activity relationships in plasmonic PTT and informs the rational design of next-generation photothermal nanotherapeutics.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"1-21"},"PeriodicalIF":3.6,"publicationDate":"2026-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148706979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polystyrene nanoplastics promote tumor lung metastasis by inducing sodium overload in macrophages in an NMDAR-dependent way. 聚苯乙烯纳米塑料通过nmdar依赖性诱导巨噬细胞钠超载促进肿瘤肺转移。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2026-08-01 Epub Date: 2026-05-19 DOI: 10.1080/17435390.2026.2669645

Lin Li, Zeyan Li, Na Ji, Yuling Chen, Xiaoling Zhang, Ziye Li, Qi Huang, Xiaoxuan Wang, Yuexuan Gao, Tianyuan Chen, Yilin Jiang, Bohua Li, Jinjue Zhang, Shanshan He, Qing Lin, Xiangyi Ren, Jing Li, Zhirong Zhang, Ling Zhang

{"title":"Polystyrene nanoplastics promote tumor lung metastasis by inducing sodium overload in macrophages in an NMDAR-dependent way.","authors":"Lin Li, Zeyan Li, Na Ji, Yuling Chen, Xiaoling Zhang, Ziye Li, Qi Huang, Xiaoxuan Wang, Yuexuan Gao, Tianyuan Chen, Yilin Jiang, Bohua Li, Jinjue Zhang, Shanshan He, Qing Lin, Xiangyi Ren, Jing Li, Zhirong Zhang, Ling Zhang","doi":"10.1080/17435390.2026.2669645","DOIUrl":"10.1080/17435390.2026.2669645","url":null,"abstract":"Micro- and nanoplastics (MNPs) are increasingly recognized as ubiquitous environmental pollutants; however, their role in tumor metastasis remains poorly understood. This study aimed to investigate the impact of polystyrene nanoplastics on breast cancer lung metastasis and to elucidate the underlying mechanisms. Using a mouse model of breast cancer, we demonstrated that inhalation of amino-modified polystyrene nanoplastics (NPS) significantly increased the number of metastatic nodules in the lungs. Mechanistically, NPS were found to bind integrins on the macrophage membrane and activated membrane-localized N-methyl-D-aspartate receptor (NMDAR), thereby inducing intracellular sodium overload. This process led to macrophage swelling, detachment, and subsequent pulmonary inflammation. Importantly, pharmacological inhibition or genetic knockdown of NMDARs effectively suppressed NPS-induced sodium influx and macrophage death, and markedly alleviated pulmonary inflammation in vivo. Collectively, these findings reveal that nanoplastics promote lung metastasis by disrupting cellular sodium homeostasis and thereby providing new insights into the potential health risks associated with nanoplastics exposure.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"260-277"},"PeriodicalIF":3.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147974112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pd@CeO₂ core-shell heteronanostructures with enhanced multienzyme activity for macrophage polarization and in flammation alleviation. Pd@CeO2具有增强巨噬细胞极化和减轻炎症的多酶活性的核壳异质结构。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2026-08-01 Epub Date: 2026-04-17 DOI: 10.1080/17435390.2026.2656691

Yanting Zhan, Yiyang Wu, Chanting Huang, Siyi Liu, Ke Ma, Li Zheng, Zainen Qin

{"title":"Pd@CeO2 core-shell heteronanostructures with enhanced multienzyme activity for macrophage polarization and in flammation alleviation.","authors":"Yanting Zhan, Yiyang Wu, Chanting Huang, Siyi Liu, Ke Ma, Li Zheng, Zainen Qin","doi":"10.1080/17435390.2026.2656691","DOIUrl":"10.1080/17435390.2026.2656691","url":null,"abstract":"The high expression of classically activated macrophages (M1) subtypes and the elevated levels of reactive oxygen species (ROS) are specifically observed in osteoarthritis (OA) patients. In this study, we synthesized a Pd@CeO2 nanozyme with a core-shell hetero-nanostructure that enhances enzyme activity through shell-core electron transfer. The goal of this work was to look into the potential of Pd@CeO2 nanozyme in scavenging ROS and regulating macrophages for the treatment of OA. The results demonstrated that the Pd@CeO2 nanozyme displayed multiple enzyme-like antioxidative activities, efficiently eliminating excessive intracellular ROS. This resulted in a significant decrease in the release of pro-inflammatory cytokines, consequently leading to a decline in the presence of M1-type macrophages. Furthermore, the Pd@CeO2 nanozyme showed excellent potential in promoting the shift of macrophages toward the M2 phenotype. Additionally, the factors secreted from the cell supernatant of Pd@CeO2-treated macrophages treated with Pd@CeO2 demonstrated anti-inflammatory properties in the chondrocytes located within inflamed synovial joints. These findings offer valuable insights into the advancement of effective enzyme-mimetic therapies for the treatment of OA.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"244-259"},"PeriodicalIF":3.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative entomotoxic effects of nanosilica and microsilica on Crocidolomia pavonana larvae. 纳米二氧化硅和微二氧化硅对黄颡鱼幼虫的虫毒效果比较。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2026-08-01 Epub Date: 2026-05-03 DOI: 10.1080/17435390.2026.2653757

Melanie Melanie, Shofi Afiya Syahbani, Wawan Hermawan, Yolani Syaputri, Ferry Faizal, Noto Susanto Gultom

{"title":"Comparative entomotoxic effects of nanosilica and microsilica on Crocidolomia pavonana larvae.","authors":"Melanie Melanie, Shofi Afiya Syahbani, Wawan Hermawan, Yolani Syaputri, Ferry Faizal, Noto Susanto Gultom","doi":"10.1080/17435390.2026.2653757","DOIUrl":"10.1080/17435390.2026.2653757","url":null,"abstract":"Nanosilica represents a promising material for insect pest management. This study compared the physicochemical properties and entomotoxic effects of nanosilica and microsilica powders against Crocidolomia pavonana larvae. The X-Ray-Fluorescence and X-Ray-Diffraction analysis confirmed higher purity in nanosilica (99.9%) than in microsilica (86.2%), with both showing amorphous structures. Particle size distribution and morphological analysis showed that nanosilica had an average particle diameter of 206.7 ± 127.7 nm, while microsilica measured 1144 ± 290.6 nm and formed a semi-spherical aggregate. Brunauer-Emmett-Teller revealed that nanosilica had a larger pore surface area (317 m2/g), total pore volume (1.539 cm³/g), and an average pore diameter (13.123 nm) compared to microsilica. Dust spray method confirmed that nanosilica had higher entomotoxicity than microsilica against C. pavonana 2nd instar larvae. This was observed from the 100% mortality achieved by nanosilica at all concentrations (N1-N4) at 72 hours (P < 0.05), whereas a similar trend was only observed at the highest microsilica concentration (M4). The median lethal concentration analysis showed that microsilica was more toxic at 24 hours (LC50 = 0.395 mg/cm2), whereas nanosilica exhibited higher toxicity at 48 and 72 hours with a lower LC50 value of 0.137 mg/cm2 and 0.106 mg/cm2. The shortest median lethal time was recorded for N4 (LT50 = 26.85 hours) treatment, indicating a faster lethal effect than M4 (LT50 = 30.91 hours) treatment. The morphological alterations in larvae included desiccation, cuticle damage, and nanosilica entrapped in the spiracle trichome, which potentially led to an obstruction. The results showed the strong insecticidal potential of nanosilica as an alternative for managing cabbage pests.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"231-243"},"PeriodicalIF":3.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147817892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated human-relevant NAMs for biomedical evaluation of selenium nanoparticles linking epithelial barrier translocation to neuronal effects. 硒纳米颗粒连接上皮屏障易位和神经元效应的生物医学评价的综合人类相关NAMs。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2026-07-24 DOI: 10.1080/17435390.2026.2702327

Ivan Mamić, Pamina Weber, Maja Beus, Nikolina Kalčec, Nikolina Peranić, Charlotte B A Stoffels, Tommaso Serchi, Ivana Vinković Vrček

{"title":"Integrated human-relevant NAMs for biomedical evaluation of selenium nanoparticles linking epithelial barrier translocation to neuronal effects.","authors":"Ivan Mamić, Pamina Weber, Maja Beus, Nikolina Kalčec, Nikolina Peranić, Charlotte B A Stoffels, Tommaso Serchi, Ivana Vinković Vrček","doi":"10.1080/17435390.2026.2702327","DOIUrl":"https://doi.org/10.1080/17435390.2026.2702327","url":null,"abstract":"New Approach Methodologies (NAMs) are increasingly needed to evaluate the safety and efficacy of nanomaterials in biomedicine while generating human-relevant evidence and reducing reliance on animal studies. For therapeutic nanoformulations, single in vitro assays often fail to capture the sequence of events that determines both functional performance and potential adverse outcomes, including interaction with exposure interfaces, epithelial transport, and response at the target tissue. Here, we present an integrated human-relevant NAMs workflow to evaluate selenium nanoparticles (SeNPs) stabilised with polyvinylpyrrolidone (PVP) or polysorbate 20 (Tween). These SeNPs were used as a relevant nanomedical case study, as such systems are increasingly being designed with antioxidant, neuroprotective, and drug-delivery properties. The workflow combined three complementary in vitro models: a tri-culture alveolar barrier at a semi-air-liquid interface, a mucus-competent intestinal barrier with continuous transepithelial electrical resistance monitoring, and differentiated dopaminergic-like SH-SY5Y neurons, representing two major therapeutic administration interfaces and a disease-relevant downstream neuronal target. Across models, SeNPs showed coating- and cell-type-dependent cytotoxicity, preserved barrier integrity at non-cytotoxic doses, and exhibited measurable basolateral transport. Neuronal uptake was confirmed, and both SeNP formulations attenuated L-dopa-induced oxidative stress without eliciting measurable pro-inflammatory cytokine release at effective concentrations. By linking two administration-relevant epithelial barrier models with a disease-relevant neuronal target, this study demonstrates how integrated NAMs can support early de-risking, formulation prioritisation, and more informed preclinical development of nanomedical systems.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"1-24"},"PeriodicalIF":3.6,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148579504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gold-modified superparamagnetic iron oxide nanoparticles: Magnetic accumulation under flow conditions and toxicity evaluation in vitro and in vivo. 金修饰的超顺磁性氧化铁纳米颗粒：流动条件下的磁积累及体外和体内毒性评价。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2026-06-01 Epub Date: 2026-04-20 DOI: 10.1080/17435390.2026.2652859

Hatice Genç, René Stein, Andreas Ettner-Sitter, Teresa Siegert, Mehtap Civelek, Christoph Alexiou, Stefan Lyer, Panagiotis Neofytou, Silke Haerteis, Iwona Cicha

{"title":"Gold-modified superparamagnetic iron oxide nanoparticles: Magnetic accumulation under flow conditions and toxicity evaluation in vitro and in vivo.","authors":"Hatice Genç, René Stein, Andreas Ettner-Sitter, Teresa Siegert, Mehtap Civelek, Christoph Alexiou, Stefan Lyer, Panagiotis Neofytou, Silke Haerteis, Iwona Cicha","doi":"10.1080/17435390.2026.2652859","DOIUrl":"10.1080/17435390.2026.2652859","url":null,"abstract":"Superparamagnetic iron oxide nanoparticles (SPIONs) enable enhanced magnetic drug targeting and theranostic applications. This study investigated potential toxic effects of SPIONs developed for drug delivery applications. Gold-modified SPIONs (SPION-Au-Cit) produced via alkaline precipitation were analyzed in terms of their hydrodynamic size, ζ-potential, magnetic susceptibility, and crystal structure. Biocompatibility of SPION-Au-Cit was tested in vitro in human umbilical vascular endothelial cells (HUVECs) and primary human fibroblasts, as well as in vivo, in chick embryos. SPION-Au-Cit had a hydrodynamic diameter of 120 nm and ζ potential of -60 mV at pH 7. In the flow cytometric analyses, SPION-Au-Cit were well tolerated by primary human fibroblasts up to a concentration of 25 µg Fe/mL, but were toxic to HUVECs at and above 10 µg Fe/mL. In contrast, endothelial toxicity was less pronounced in real-time cell analysis and wound-healing assays, although the particles were strongly internalized by HUVECs. The injection of SPION-Au-Cit (45 µg) into chick embryos resulted in progressive hemoglobin oxidation, leading to high fetal toxicity. The magnetic accumulation of SPIONs under flow conditions was investigated in vitro and modeled in silico. Under arterial-like flow conditions in vitro, a strong time-dependent magnetic accumulation of SPION-Au-Cit was observed at a concentration of 2.5 µg Fe/mL. SPION-Au-Cit showed pronounced toxicity in endothelial cells and chick embryos. Although the magnetic properties of SPION-Au-Cit enable their effective accumulation even under flow conditions, their potential biomedical applications would require both precise targeting and careful dose-finding studies to prevent harmful off-target effects.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"208-229"},"PeriodicalIF":3.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0