遗传故事:氧化石墨烯对果蝇F1到F4代的多代可逆影响。

IF 3.4 3区 医学 Q3 NANOSCIENCE & NANOTECHNOLOGY
Nanotoxicology Pub Date : 2025-08-01 Epub Date: 2025-08-28 DOI:10.1080/17435390.2025.2549545
Avnika Singh Anand, Kalyani Verma, Amitabh, Dipti N Prasad, Ekta Kohli
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引用次数: 0

摘要

随着氧化石墨烯(GO)应用的不断深入,其主要的阻碍因素是其毒性。了解对父母一代的直接影响以及对后代的长期多代影响是至关重要的。本文研究了氧化石墨烯在黑腹果蝇(Drosophila melanogaster)模式生物中从亲代到后代(F0, F1, F2, F3至F4)的多代效应。果蝇通过摄入法暴露于氧化石墨烯浓度为50µg/mL、100µg/mL和250µg/mL的环境中。通过攀爬试验、长寿试验、氧化应激和后代表型筛选,研究不同水平氧化石墨烯对后代的影响。结果显示,接触氧化石墨烯的果蝇攀爬能力明显下降,氧化应激(F2)增加,亲本(F0)到后代(F1, F2)的寿命缩短。至关重要的是,这些毒性作用在后代(F3-F4)中的逆转表明,苍蝇通过适应机制的发展克服了长时间接触氧化石墨烯的有害影响。这些发现强调了研究纳米材料(NMs)的多代效应的重要性,因为由于在后代中出现适应性反应,最初的毒性可能不会持续一段时间。了解和减轻氧化石墨烯的毒性对其在各个领域的安全应用至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The inherited story: multigenerational reversible effects of graphene oxide from F1 to F4 generations of Drosophila melanogaster.

With advances in the application of graphene oxide (GO), the major hindering factor is its toxicity. It is crucial to understand the immediate effects on the parent generation as well as the long-term multigenerational effects on subsequent generations. In this paper we investigated the multigenerational effect of GO from the parent to subsequent generations (F0, F1, F2, F3 to F4) in Drosophila melanogaster model organism. Flies were exposed to GO through the ingestion method at concentrations ranging from 50 µg/mL, 100 µg/mL, and 250 µg/mL. The effects of GO were studied at different levels via climbing assay, longevity assay, oxidative stress and phenotypic screening in subsequent generations. Significant declines were observed in the climbing ability, an increase in oxidative stress (F2), and a decrease in lifespan of the parent (F0) to progeny (F1, F2) flies exposed to GO. Critically, the reversal of these toxic effects in the later generations (F3-F4), suggests the development of adaptive mechanisms through which flies overcome the detrimental impacts of prolonged GO exposure. These findings underscore the importance of examining the multigenerational effects of nanomaterials (NMs), as the initial toxicity may not persist over time due to the emergence of adaptive responses in subsequent generations. Understanding and mitigating the toxicity of GO over generations is essential for its safe application in various fields.

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来源期刊
Nanotoxicology
Nanotoxicology 医学-毒理学
CiteScore
10.10
自引率
4.00%
发文量
45
审稿时长
3.5 months
期刊介绍: Nanotoxicology invites contributions addressing research relating to the potential for human and environmental exposure, hazard and risk associated with the use and development of nano-structured materials. In this context, the term nano-structured materials has a broad definition, including ‘materials with at least one dimension in the nanometer size range’. These nanomaterials range from nanoparticles and nanomedicines, to nano-surfaces of larger materials and composite materials. The range of nanomaterials in use and under development is extremely diverse, so this journal includes a range of materials generated for purposeful delivery into the body (food, medicines, diagnostics and prosthetics), to consumer products (e.g. paints, cosmetics, electronics and clothing), and particles designed for environmental applications (e.g. remediation). It is the nano-size range if these materials which unifies them and defines the scope of Nanotoxicology . While the term ‘toxicology’ indicates risk, the journal Nanotoxicology also aims to encompass studies that enhance safety during the production, use and disposal of nanomaterials. Well-controlled studies demonstrating a lack of exposure, hazard or risk associated with nanomaterials, or studies aiming to improve biocompatibility are welcomed and encouraged, as such studies will lead to an advancement of nanotechnology. Furthermore, many nanoparticles are developed with the intention to improve human health (e.g. antimicrobial agents), and again, such articles are encouraged. In order to promote quality, Nanotoxicology will prioritise publications that have demonstrated characterisation of the nanomaterials investigated.
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