Nanotoxicology最新文献_第10页

Dependence of diamond nanoparticle cytotoxicity on physicochemical parameters: comparative studies of glioblastoma, breast cancer, and hepatocellular carcinoma cell lines. 金刚石纳米颗粒细胞毒性对理化参数的依赖性:胶质母细胞瘤、乳腺癌和肝癌细胞系的比较研究。

IF 5 3区医学

Nanotoxicology Pub Date : 2023-06-01 DOI: 10.1080/17435390.2023.2218925

Barbara Wójcik, Katarzyna Zawadzka, Sławomir Jaworski, Marta Kutwin, Malwina Sosnowska, Agnieszka Ostrowska, Marta Grodzik, Artur Małolepszy, Marta Mazurkiewicz-Pawlicka, Mateusz Wierzbicki

{"title":"Dependence of diamond nanoparticle cytotoxicity on physicochemical parameters: comparative studies of glioblastoma, breast cancer, and hepatocellular carcinoma cell lines.","authors":"Barbara Wójcik, Katarzyna Zawadzka, Sławomir Jaworski, Marta Kutwin, Malwina Sosnowska, Agnieszka Ostrowska, Marta Grodzik, Artur Małolepszy, Marta Mazurkiewicz-Pawlicka, Mateusz Wierzbicki","doi":"10.1080/17435390.2023.2218925","DOIUrl":"https://doi.org/10.1080/17435390.2023.2218925","url":null,"abstract":"Reports on the cytotoxicity of diamond nanoparticles (ND) are ambiguous and depend on the physicochemical properties of the material and the tested cell lines. Thus, the aim of this research was to evaluate the influence of thirteen types of diamond nanoparticles, differing in production method, size, and surface functional groups, on their cytotoxicity against four tumor cell lines (T98G, U-118 MG, MCF-7, and Hep G2) and one non-tumor cell line (HFF-1). In order to understand the dependence of diamond nanoparticles on physicochemical properties, the following parameters were analyzed: viability, cell membrane damage, morphology, and the level of intracellular general ROS and mitochondrial superoxide. The performed analyses revealed that all diamond nanoparticles showed no toxicity to MCF-7, Hep G2, and HFF-1 cells. In contrast, the same nanomaterials were moderately toxic for the glioblastoma T98G and U-118 MG cell lines. In general, the effect of the production method did not influence ND toxicity. Some changes in cell response after treatment with modified nanomaterials were observed, with the presence of carboxyl groups having a more detrimental effect than the presence of other functional groups. Although nanoparticles of different sizes caused similar toxicity, nanomaterials with bigger particles caused a more pronounced effect.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":"17 4","pages":"310-337"},"PeriodicalIF":5.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9851735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Impact of physico-chemical properties on the toxicological potential of reduced graphene oxide in human bronchial epithelial cells. 物理化学性质对人支气管上皮细胞中还原氧化石墨烯毒理学潜力的影响。

IF 5 3区医学

Nanotoxicology Pub Date : 2023-06-01 Epub Date: 2023-10-17 DOI: 10.1080/17435390.2023.2265465

Adriana Rodríguez-Garraus, Clara Passerino, Gerard Vales, Michela Carlin, Satu Suhonen, Aurelia Tubaro, Julio Gómez, Marco Pelin, Julia Catalán

{"title":"Impact of physico-chemical properties on the toxicological potential of reduced graphene oxide in human bronchial epithelial cells.","authors":"Adriana Rodríguez-Garraus, Clara Passerino, Gerard Vales, Michela Carlin, Satu Suhonen, Aurelia Tubaro, Julio Gómez, Marco Pelin, Julia Catalán","doi":"10.1080/17435390.2023.2265465","DOIUrl":"10.1080/17435390.2023.2265465","url":null,"abstract":"The increasing use of graphene-based materials (GBM) requires their safety evaluation, especially in occupational settings. The same physico-chemical (PC) properties that confer GBM extraordinary functionalities may affect the potential toxic response. Most toxicity assessments mainly focus on graphene oxide and rarely investigate GBMs varying only by one property. As a novelty, the present study assessed the in vitro cytotoxicity and genotoxicity of six reduced graphene oxides (rGOs) with different PC properties in the human bronchial epithelial 16HBE14o - cell line. Of the six materials, rGO1-rGO4 only differed in the carbon-to-oxygen (C/O) content, whereas rGO5 and rGO6 were characterized by different lateral size and number of layers, respectively, but similar C/O content compared with rGO1. The materials were characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, laser diffraction and dynamic light scattering, and Brunauer-Emmett-Teller analysis. Cytotoxicity (Luminescent Cell Viability and WST-8 assays), the induction of reactive oxygen species (ROS; 2',7'-dichlorofluorescin diacetate-based assay), the production of cytokines (enzyme-linked immunosorbent assays) and genotoxicity (comet and micronucleus assays) were evaluated. Furthermore, the internalization of the materials in the cells was confirmed by laser confocal microscopy. No relationships were found between the C/O ratio or the lateral size and any of the rGO-induced biological effects. However, rGO of higher oxygen content showed higher cytotoxic and early ROS-inducing potential, whereas genotoxic effects were observed with the rGO of the lowest density of oxygen groups. On the other hand, a higher number of layers seems to be associated with a decreased potential for inducing cytotoxicity and ROS production.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":" ","pages":"471-495"},"PeriodicalIF":5.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41166896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-walled carbon nanotubes induce arachidonate 5-lipoxygenase expression and enhance the polarization and function of M1 macrophages in vitro. 多壁碳纳米管在体外诱导花生四烯酸5-脂氧合酶表达，增强M1巨噬细胞的极化和功能。

IF 3.6 3区医学

Nanotoxicology Pub Date : 2023-04-01 Epub Date: 2023-04-28 DOI: 10.1080/17435390.2023.2204161

Chol Seung Lim, Brandon Veltri, Michael Kashon, Dale W Porter, Qiang Ma

{"title":"Multi-walled carbon nanotubes induce arachidonate 5-lipoxygenase expression and enhance the polarization and function of M1 macrophages in vitro.","authors":"Chol Seung Lim, Brandon Veltri, Michael Kashon, Dale W Porter, Qiang Ma","doi":"10.1080/17435390.2023.2204161","DOIUrl":"10.1080/17435390.2023.2204161","url":null,"abstract":"Fibrogenic carbon nanotubes (CNTs) induce the polarization of M1 and M2 macrophages in mouse lungs. Polarization of the macrophages regulates the production of proinflammatory and pro-resolving lipid mediators (LMs) to mediate acute inflammation and its resolution in a time-dependent manner. Here we examined the molecular mechanism by which multi-walled CNTs (MWCNTs, Mitsui-7) induce M1 polarization in vitro. Treatment of murine macrophages (J774A.1) with Mitsui-7 MWCNTs increased the expression of Alox5 mRNA and protein in a concentration- and time-dependent manner. The MWCNTs induced the expression of CD68 and that induction persisted for up to 3 days post-exposure. The expression and activity of inducible nitric oxide synthase, an intracellular marker of M1, were increased by MWCNTs. Consistent with M1 polarization, the MWCNTs induced the production and secretion of proinflammatory cytokines tumor necrosis factor-α and interleukin-1β, and proinflammatory LMs leukotriene B4 (LTB4) and prostaglandin E2 (PGE2). The cell-free media from MWCNT-polarized macrophages induced the migration of neutrophilic cells (differentiated from HL-60), which was blocked by Acebilustat, a specific leukotriene A4 hydrolase inhibitor, or LY239111, an LTB4 receptor antagonist, but not NS-398, a cyclooxygenase 2 inhibitor, revealing LTB4 as a major mediator of neutrophil chemotaxis from MWCNT-polarized macrophages. Knockdown of Alox5 using specific small hairpin-RNA suppressed MWCNT-induced M1 polarization, LTB4 secretion, and migration of neutrophils. Taken together, these findings demonstrate the polarization of M1 macrophages by Mitsui-7 MWCNTs in vitro and that induction of Alox5 is an important mechanism by which the MWCNTs promote proinflammatory responses by boosting M1 polarization and production of proinflammatory LMs.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":"17 3","pages":"249-269"},"PeriodicalIF":3.6,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10263170/pdf/nihms-1899756.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9678401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced toxicity of 2,2-bis(chloromethyl) trimethylene bis[bis(2-chloroethyl) phosphate] (V6) by nanopolystyrene particles towards HeLa cells. 纳米聚苯乙烯颗粒增强2,2-二(氯甲基)三亚甲基二[二(2-氯乙基)磷酸](V6)对HeLa细胞的毒性。

IF 5 3区医学

Nanotoxicology Pub Date : 2023-04-01 DOI: 10.1080/17435390.2023.2203238

Zheng Zhong, Xin Liu, Yiming Ruan, Ziwei Li, Junxian Li, Lili Sun, Sen Hou

{"title":"Enhanced toxicity of 2,2-bis(chloromethyl) trimethylene bis[bis(2-chloroethyl) phosphate] (V6) by nanopolystyrene particles towards HeLa cells.","authors":"Zheng Zhong, Xin Liu, Yiming Ruan, Ziwei Li, Junxian Li, Lili Sun, Sen Hou","doi":"10.1080/17435390.2023.2203238","DOIUrl":"https://doi.org/10.1080/17435390.2023.2203238","url":null,"abstract":"2,2-bis(chloromethyl) trimethylene bis[bis(2-chloroethyl) phosphate] (V6) has been widely used as an additive in a variety of plastics due to its extremely low toxicity. However, we showed in the study that once mixed with nanopolystyrene particles (NPs), the nontoxic V6 could exhibit significant toxicity to HeLa cells. The enhanced toxicity was much higher than the toxicity of NPs alone and was related to the size of NPs. The mixture of V6 and small polystyrene NPs (10 nm and 15 nm in radius) showed obvious toxicity to HeLa cells. The toxicity increased with the concentrations of both V6 and NPs. On the contrary, the mixture of V6 and larger NPs (25 nm, 50 nm, 100 nm, and 500 nm in radius) showed almost no toxicity even at extremely high concentrations (NPs: 100 mg/L; V6: 50 mg/L). The small NPs could enter the cells and accumulated in cytoplasm. However, the larger NPs did not distribute inside the cells. NPs efficiently adsorbed V6 on the surface. The mechanism of the enhanced toxicity was attributed to the increased intracellular reactive oxygen species (ROS) production and the regulation of gene expression concerning apoptosis and ROS scavenging. Our study not only showed that a safe chemical V6 could be turned to be toxic by NPs, but also pointed out a potential risk caused by the joint toxicity of 'safe' chemicals and plastic particles with small size.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":"17 3","pages":"203-217"},"PeriodicalIF":5.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9459184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Needlelike, short and thin multi-walled carbon nanotubes: comparison of effects on wild type and p53^+/- rat lungs. 针状、短和薄的多壁碳纳米管:对野生型和p53+/-大鼠肺的影响比较。

IF 5 3区医学

Nanotoxicology Pub Date : 2023-04-01 DOI: 10.1080/17435390.2023.2204933

Hélène Barthel, Sylvie Sébillaud, Mylène Lorcin, Henrik Wolff, Stéphane Viton, Frédéric Cosnier, Laurent Gaté, Carole Seidel

{"title":"Needlelike, short and thin multi-walled carbon nanotubes: comparison of effects on wild type and p53+/- rat lungs.","authors":"Hélène Barthel, Sylvie Sébillaud, Mylène Lorcin, Henrik Wolff, Stéphane Viton, Frédéric Cosnier, Laurent Gaté, Carole Seidel","doi":"10.1080/17435390.2023.2204933","DOIUrl":"https://doi.org/10.1080/17435390.2023.2204933","url":null,"abstract":"Carbon nanotubes (CNTs) are nanomaterials presenting an occupational inhalation risk during production or handling. The International Agency for Research on Cancer classified one CNT, Mitsui-7 (MWNT-7), as 'possibly carcinogenic to humans'. In recognition of their similarities, a proposal has been submitted to the risk assessment committee of ECHA to classify all fibers with 'Fibre Paradigm' (FP)-compatible dimensions as carcinogenic. However, there is a lack of clarity surrounding the toxicity of fibers that do not fit the FP criteria. In this study, we compared the effects of the FP-compatible Mitsui-7, to those of NM-403, a CNT that is too short and thin to fit the paradigm. Female Sprague Dawley rats deficient for p53 (GMO) and wild type (WT) rats were exposed to the two CNTs (0.25 mg/rat/week) by intratracheal instillation. Animals (GMO and WT) were exposed weekly for four consecutive weeks and were sacrificed 3 days or 8 months after the last instillation. Exposure to both CNTs induced acute lung inflammation. However, persistent inflammation at 8 months was only observed in the lungs of rats exposed to NM-403. In addition to the persistent inflammation, NM-403 stimulated hyperplasic changes in rat lungs, and no adenomas or carcinomas were detected. The degree and extent of hyperplasia was significantly more pronounced in GMO rats. These results suggest that CNT not meeting the FP criteria can cause persistent inflammation and hyperplasia. Consequently, their health effects should be carefully assessed.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":"17 3","pages":"270-288"},"PeriodicalIF":5.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9863831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Assessing regulated cell death modalities as an efficient tool for in vitro nanotoxicity screening: a review. 评估作为体外纳米毒性筛选有效工具的受调控细胞死亡模式:综述

IF 5 3区医学

Nanotoxicology Pub Date : 2023-04-01 DOI: 10.1080/17435390.2023.2203239

Anton Tkachenko, Anatolii Onishchenko, Valeriy Myasoedov, Svetlana Yefimova, Ondrej Havranek

{"title":"Assessing regulated cell death modalities as an efficient tool for in vitro nanotoxicity screening: a review.","authors":"Anton Tkachenko, Anatolii Onishchenko, Valeriy Myasoedov, Svetlana Yefimova, Ondrej Havranek","doi":"10.1080/17435390.2023.2203239","DOIUrl":"https://doi.org/10.1080/17435390.2023.2203239","url":null,"abstract":"Nanomedicine is a fast-growing field of nanotechnology. One of the major obstacles for a wider use of nanomaterials for medical application is the lack of standardized toxicity screening protocols for assessing the safety of newly synthesized nanomaterials. In this review, we focus on less frequently studied nanomaterials-induced regulated cell death (RCD) modalities, including eryptosis, necroptosis, pyroptosis, and ferroptosis, as a tool for in vitro nanomaterials safety evaluation. We summarize the latest insights into the mechanisms that mediate these RCDs in response to nanomaterials exposure. Comprehensive data from reviewed studies suggest that ROS (reactive oxygen species) overproduction and ROS-mediated pathways play a central role in nanomaterials-induced RCDs activation. On the other hand, studies also suggest that individual properties of nanomaterials, including size, shape, or surface charge, could determine specific toxicity pathways with consequent RCD induction as well. We anticipate that the evaluation of RCDs can become one of the mechanism-based screening methods in nanotoxicology. In addition to the toxicity assessment, evaluation of necroptosis-, pyroptosis-, and ferroptosis-promoting capacity of nanomaterials could simultaneously provide useful information for specific medical applications as could be their anti-tumor potential. Moreover, a detailed understanding of molecular mechanisms driving nanomaterials-mediated induction of immunogenic RCDs will substantially aid novel anti-tumor nanodrugs development.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":"17 3","pages":"218-248"},"PeriodicalIF":5.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9458679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Algal extracellular polymeric substances (algal-EPS) for mitigating the combined toxic effects of polystyrene nanoplastics and nano-TiO₂ in Chlorella sp. 藻类胞外聚合物质(藻类- eps)减轻聚苯乙烯纳米塑料和纳米tio2对小球藻的联合毒性作用。

IF 5 3区医学

Nanotoxicology Pub Date : 2023-03-01 DOI: 10.1080/17435390.2023.2179438

Lokeshwari Natarajan, M Annie Jenifer, Willie J G M Peijnenburg, Amitava Mukherjee

{"title":"Algal extracellular polymeric substances (algal-EPS) for mitigating the combined toxic effects of polystyrene nanoplastics and nano-TiO2 in Chlorella sp.","authors":"Lokeshwari Natarajan, M Annie Jenifer, Willie J G M Peijnenburg, Amitava Mukherjee","doi":"10.1080/17435390.2023.2179438","DOIUrl":"https://doi.org/10.1080/17435390.2023.2179438","url":null,"abstract":"The continuous release of nanoparticles and nanoplastics into the marine environment necessitates the examination of their combined effects in marine organisms. Natural Organic Matter (NOM) can significantly influence the behavior of nanomaterials in the marine environment. The present study explores the effects of algal Extracellular Polymeric Substances (EPS) in reducing the combined toxic effects of three different polystyrene nanoplastics (PSNPs)- aminated (NH2-PSNPs), carboxylated (COOH-PSNPs), and plain PSNPs - and P25 titanium dioxide nanoparticles (Nano-TiO2) towards the marine alga, Chlorella sp. Two doses (0.25 and 2.5 mg/L) of nano-TiO2 mixed with the PSNPs (1 mg/L) were employed. The COOH-PSNPs with 2.5 mg/L nano-TiO2 exhibited higher growth inhibition toward algal cells. Addition of algal EPS to the mixture of NMs decreased the negative effect significantly. The mean hydrodynamic diameter increased significantly from 666 to 797 nm and 1248 to 3589 nm at concentrations 0.25 and 2.5 mg/L, respectively when the mixtures of nano-TiO2 and COOH-PSNPs were incubated with the algal EPS. In comparison to the pristine NMs, the EPS-NMs were found to significantly reduce the superoxide and hydroxyl radical production. The results were further validated with the estimation of lipid peroxidation (LPO), esterase activity, photosynthetic efficiency, and membrane permeability in the cells. The major outcomes from this study highlight the role of algal EPS in significantly reducing the toxic impact of binary mixture of NMs in marine organisms.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":"17 2","pages":"143-156"},"PeriodicalIF":5.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9460717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Ferritinophagy was involved in long-term SiNPs exposure induced ferroptosis and liver fibrosis. 铁蛋白自噬参与长期暴露于SiNPs诱导的铁下垂和肝纤维化。

IF 5 3区医学

Nanotoxicology Pub Date : 2023-03-01 DOI: 10.1080/17435390.2023.2197055

Qingqing Liang, Yuexiao Ma, Fenghong Wang, Mengqi Sun, Lisen Lin, Tianyu Li, Junchao Duan, Zhiwei Sun

{"title":"Ferritinophagy was involved in long-term SiNPs exposure induced ferroptosis and liver fibrosis.","authors":"Qingqing Liang, Yuexiao Ma, Fenghong Wang, Mengqi Sun, Lisen Lin, Tianyu Li, Junchao Duan, Zhiwei Sun","doi":"10.1080/17435390.2023.2197055","DOIUrl":"https://doi.org/10.1080/17435390.2023.2197055","url":null,"abstract":"SiNPs could induce liver fibrosisinvivo, but the mechanism was not completely clear. This study focused on exploring whether long-term SiNPs exposure at human-related exposure dosage could lead to ferritinophagy-mediated ferroptosis and liver fibrosis. In vivo, long-term SiNPs exposure induced liver fibrosis inrats accompanied by ferritinophagy and ferroptosis in hepatocytes. Interestingly, the progression of liver fibrosis was alleviated after exposure cessation and recovery, meanwhile ferritinophagy and ferroptosis were not further activated. In vitro, after long-term SiNPs exposure, the mitochondrial membrane ruptured, lipid peroxidation intensified, the level of redox active iron increased and the repair protein of lipid peroxidation were consumed in L-02 cells, demonstrating ferroptosis occurrence. Notably, NCOA4 knockdown inhibited ferritin degradation, alleviated the increase of intracellular ferrous iron level, reduced lipid peroxidation and the depletion of glutathione peroxidase 4 (GPX4). In conclusion, ferritinophagy mediated by NCOA4 was responsible for long-term SiNPs exposure induced hepatocytes ferroptosis and liver fibrosis, which provided a scientific basis for toxicological assessment of SiNPs and would be benefited for the safety design of SiNPs-based products.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":"17 2","pages":"157-175"},"PeriodicalIF":5.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9827086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

TiO₂ nanostructures - a double edged sword: current progress on their role in stem cells' differentiation, cancer therapy, and their toxicity issues. 二氧化钛纳米结构-一把双刃剑:它们在干细胞分化、癌症治疗及其毒性问题中的作用的最新进展

IF 5 3区医学

Nanotoxicology Pub Date : 2023-03-01 DOI: 10.1080/17435390.2023.2199858

Saravanan Ramesh, Kavitha Govarthanan, Arunkumar Palaniappan

{"title":"TiO2 nanostructures - a double edged sword: current progress on their role in stem cells' differentiation, cancer therapy, and their toxicity issues.","authors":"Saravanan Ramesh, Kavitha Govarthanan, Arunkumar Palaniappan","doi":"10.1080/17435390.2023.2199858","DOIUrl":"https://doi.org/10.1080/17435390.2023.2199858","url":null,"abstract":"Titanium dioxide (TiO2)-based nanostructures have wide applications in cosmetics, toothpastes, pharmaceuticals, coatings, papers, inks, plastics, food products, textiles, and many others. Recently, they have also been found to have huge potential as stem cells' differentiation agents as well as stimuli-responsive drug delivery systems in cancer therapy. In this review, we present some of the recent progress in the role of TiO2-based nanostructures toward the above-mentioned applications. We also present recent studies on the toxicity issues of these nanomaterials and the mechanisms behind the toxicity issues. We have reviewed the recent progress of TiO2-based nanostructures on their stem cells' differentiation potentials, their photo- and sono-dynamic capabilities, as stimuli-responsive drug delivery systems, and finally their toxicity issues with mechanistic understanding on the same. We believe that this review will help researchers be aware of the latest progress in the applications as well as few toxicity issues associated with TiO2-based nanostructures, which will help them design better nanomedicine for future applications.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":"17 2","pages":"176-201"},"PeriodicalIF":5.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9462079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of genotoxicity induced by subchronic exposure to graphene in HaCaT human skin cell line. 亚慢性接触石墨烯对HaCaT人皮肤细胞系的遗传毒性评估。

IF 5 3区医学

Nanotoxicology Pub Date : 2023-02-01 DOI: 10.1080/17435390.2023.2183653

Javier Frontiñan-Rubio, Sonia García-Carpintero, Viviana Jehová González, Ester Vázquez, Mario Durán-Prado

{"title":"Assessment of genotoxicity induced by subchronic exposure to graphene in HaCaT human skin cell line.","authors":"Javier Frontiñan-Rubio, Sonia García-Carpintero, Viviana Jehová González, Ester Vázquez, Mario Durán-Prado","doi":"10.1080/17435390.2023.2183653","DOIUrl":"https://doi.org/10.1080/17435390.2023.2183653","url":null,"abstract":"The applications of graphene-based materials (GBMs) and their processing involve prolonged contact with cellular barriers such as human skin. Even though the potential cytotoxicity of graphene has been studied in recent years, the impact of long-term graphene exposure has rarely been explored. We tested in the HaCaT epithelial cells, in vitro, the effect of subchronic treatments with sublethal doses of four different, well-characterized GBMs, two commercial graphene oxides (GO) and two few-layer graphenes (FLG). Cells were exposed weekly to low doses of the GBMs for 14 days, 30 days, 3 months, and 6 months. GBMs-cells uptake was assessed by confocal microscopy. Cell death and cell cycle were determined by fluorescence microscopy and cytometry. DNA damage was measured by comet assay and γ-H2AX staining, followed by the determination of p-p53 and p-ATR by immunolabeling. Subchronic exposure to different GBMs at noncytotoxic doses has potential genotoxic effects on HaCaT epithelial cells that can be recovered depending on the GBM and exposure time. Specifically, GO-induced genotoxicity can be detected after 14 and 30 days from treatment. At this time, FLG appears less genotoxic than GO, and cells can recover more quickly when genotoxic pressure disappears after some days of removal of the GBM. Long-term exposure, 3 and 6 months, to different GBMs induces permanent, nonreversible, genotoxic damage comparable to the exerted by arsenite. This should be considered for the production and future applications of GBMs in scenarios where low concentrations of the material interact chronically with epithelial barriers.","PeriodicalId":18899,"journal":{"name":"Nanotoxicology","volume":"17 1","pages":"42-61"},"PeriodicalIF":5.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9377899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1