Mycologia最新文献

{"title":"The genus <i>Volvariella</i> from southwestern China.","authors":"Xuan Chen, Yang-Yang Cui, Zhu L Yang","doi":"10.1080/00275514.2025.2476934","DOIUrl":"10.1080/00275514.2025.2476934","url":null,"abstract":"<p><p>In this study, morphological characteristics, ecological features, and phylogenetic analyses based on nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS), partial nuc rDNA 28S (28S), and the translation elongation factor 1-α (<i>TEF1</i>) were employed to investigate the species diversity of the genus <i>Volvariella</i> in southwestern China. Eight species are recognized, among which are five known species, namely, <i>V. bombycina, V. hypopithys, V. morozovae, V. pulla</i>, and <i>V. volvacea</i>, and three new species described here, namely, <i>V. parvirimosa, V. pilosa</i>, and <i>V. rimosa. Volvariella pulla</i> represents a species new to China. For species delimitation, the size of the basidiomes, the morphology of the pileus, the size of basidiospores, and the morphology of hymenial cystidia are informative. Taxonomic descriptions of the new species are presented, and a key to the eight species from southwestern China is provided.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"499-515"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New species of <i>Thelephora</i> (Thelephorales, Basidiomycota) associated with <i>Dicymbe</i> and <i>Aldina</i> in Guyana.","authors":"Julieta Alvarez-Manjarrez, Terry W Henkel, Ansel Duncan, M Catherine Aime, Kabir G Peay, Matthew E Smith","doi":"10.1080/00275514.2025.2472593","DOIUrl":"10.1080/00275514.2025.2472593","url":null,"abstract":"<p><p>Species of <i>Thelephora</i> Ehrh. ex Willd. (Thelephorales, Basidiomycota) are known from all continents where ectomycorrhizal (ECM) host plants occur. Although often poorly represented in sporocarp surveys due to the cryptic basidiomata of the resupinate species, belowground sequencing studies in both temperate and tropical systems have shown that thelephoroid fungi are often the dominant ECM group. In the South American lowland tropics, thelephoroid fungi remain poorly known, and very few species have been described from the region. Long-term surveys in Guyana have revealed a wide diversity of ECM fungal species in association with the ECM trees <i>Dicymbe corymbosa</i> (Fabaceae subfam. Detarioideae) and <i>Aldina insignis</i> (Benth.) Endl. (Fabaceae subfam. Papilionoideae). Thelephoroid species form a prominent component of this ECM fungal assemblage, as evidenced by their dominance in both adult tree and seedling root tip surveys and frequent occurrence as fertile basidiomata on a variety of substrata. Here we describe four new thelephoroid species from Guyana that are among the most frequently collected as basidiomata: <i>Thelephora ascendens, Thelephora compacta, Thelephora singularis</i>, and <i>Thelephora ventricobasidia</i>. Three of these species have resupinate basidiomata and would have formerly been assigned to the genus <i>Tomentella</i> Pers. ex Pat. Morphological descriptions, habit, habitat, and known distribution are provided for each new species. Sequence data for the barcode internal transcribed spacer (ITS) locus is provided for types and most other collections of the new species, and a molecular phylogenetic analysis across the Thelephoraceae corroborates their status as novel taxa.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"480-498"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity and ultramicroscopic analysis of green muscardine fungus, <i>Metarhizium anisopliae</i>, infecting the tomato fruit borer, <i>Helicoverpa armigera</i>.","authors":"Yamini Rajendran, Panthagani Venkata Koushik, Shanmugam Pagalahalli Sankaran, Murugan Marimuthu, Geetha Nandagopal, Srinivasan Thulasy, Meenakshi Periasamy, Kandan Aravindaram","doi":"10.1080/00275514.2025.2481821","DOIUrl":"https://doi.org/10.1080/00275514.2025.2481821","url":null,"abstract":"<p><p>The polyphagous insect <i>Helicoverpa armigera</i> is a devastating pest known to develop resistance to insecticides quickly. In the search for alternatives, the entomopathogenic fungus <i>Metarhizium anisopliae</i> has been found to be an eco-friendly component of integrated pest management for lepidopteran insects all over the world. On tomatoes, this pest devours both the leaves and the fruits. The present investigation was carried out to identify effective <i>M. anisopliae</i> strains against <i>H. armigera</i> through ultramicroscopic investigations. The fungal strains sourced from soil and <i>Spodoptera frugiperda</i> cadavers were subjected to molecular and morphological characterization, confirming their identity as <i>M. anisopliae</i>. Of the 10 <i>M. anisopliae</i> strains, ICAR SBI Ma 08 and ICAR SBI Ma 01 ranked first and second in efficacy against <i>H. armigera</i> second and fourth instars, respectively. The order of efficacy of other strains against <i>H. armigera</i> was ICAR SBI Ma 69 > ICAR SBI Ma 44 > ICAR SBI Ma 07 > ICAR SBI Ma 05 > ICAR SBI Ma 172 > ICAR SBI Ma 03 > ICAR SBI Ma 46 > ICAR SBI Ma 04. Ultramicroscopic examinations found the growth of mycelia in the natural openings of the larva, such as the spiracles, thorax, legs, and setal alveoli, within 168 h. The tested strains demonstrated greater effectiveness against the second instar compared with the fourth instar, suggesting that the most potent strain, ICAR SBI Ma 08, can effectively manage the early instars.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"1-17"},"PeriodicalIF":2.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The enzymatic arsenal of <i>Discula destructiva</i>: strategies for understanding and managing the dogwood anthracnose pathogen.","authors":"Marcin Nowicki, Kayleigh P Redington, Sarah L Boggess, I Shade Niece, Robert N Trigiano","doi":"10.1080/00275514.2025.2489332","DOIUrl":"https://doi.org/10.1080/00275514.2025.2489332","url":null,"abstract":"<p><p>The increasing prevalence of fungal diseases poses major threats to agricultural productivity and biodiversity, particularly in the context of plant-pathogen interactions. Understanding the mechanisms by which pathogens such as <i>Discula destructiva</i> cause disease and losses in dogwoods, important forest plants and nursery ornamental crops, is crucial for developing effective management strategies. This study focuses on the enzymatic activities of <i>D. destructiva</i>, the causal agent of dogwood anthracnose, and their role in facilitating pathogenicity in <i>Cornus</i> spp. We hypothesized that <i>D. destructiva</i> produces higher levels of extracellular hydrolytic and oxidative enzymes compared with other <i>Discula</i> species, which could enhance its ability to invade and degrade host plant tissues. Our findings revealed that isolates of <i>D. destructiva</i> exhibit elevated enzymatic activities, specifically with abundant polyphenol oxidase activity. Additionally, we assessed the influence of environmental factors, such as pH and medium composition, on pathogen growth, demonstrated that optimal growth occurs at pH 4.5, whereas alkaline conditions inhibited growth dynamics. These insights suggest that managing environmental conditions or fungicide formulations could be an effective strategy for controlling the spread of dogwood anthracnose pathogen, thereby emphasizing the importance of enzymatic profiles in fungal pathogenicity.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"1-14"},"PeriodicalIF":2.6,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing rice growth in adverse conditions: the role of dark septate endophytes in salt and water scarcity tolerance.","authors":"Esther Lalthazuali, Badaiahun Marwein, Hmingremhlua Sailo, P S Lalbiaktluanga, Mc F Vanlalmuana, Lalfakzuala Ralte","doi":"10.1080/00275514.2025.2474900","DOIUrl":"https://doi.org/10.1080/00275514.2025.2474900","url":null,"abstract":"<p><p>Global warming increasingly challenges agricultural productivity due to rising salt and water scarcity stress. In response to these challenges, the present study investigates the potential of dark septate endophytes (DSEs) from Khawrihnim Village, Mizoram, India, to enhance the tolerance of upland rice (<i>Oryza sativa</i>). Two DSE species, <i>Acrocalymma vagum</i> and <i>Alternaria chlamydospora</i>, were identified and examined for their potential enhancement of plant resilience to salt and water scarcity stress. The result showed that both DSE species substantially impacted rice seed germination, biomass accumulation, and oxidative stress responses under salt and water scarcity conditions. Biomass content was augmented under elevated stress levels (-0.90 MPa), suggesting possible stress tolerance mechanisms. Increased superoxide dismutase activity and diminished malondialdehyde levels under stress conditions underscored the DSE's capacity to alleviate reactive oxygen species and lipid peroxidation in plants. Furthermore, seed and shoot germination rates were markedly enhanced by 60% using DSEs as biofertilizers, particularly evident in direct treatments. These findings highlight the efficacy of the DSEs <i>Acrocalymma vagum</i> and <i>Alternaria chlamydospore</i> as biofertilizers to enhance plant development and resilience in adverse environmental conditions. Their capacity to augment abiotic stress resilience and promote agricultural sustainability presents a significant opportunity for enhancing crop output, especially in areas susceptible to stress.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"1-13"},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new species of <i>Periglandula</i> symbiotic with the morning glory <i>Ipomoea tricolor</i>.","authors":"Corinne M Hazel, Daniel G Panaccione","doi":"10.1080/00275514.2025.2483634","DOIUrl":"https://doi.org/10.1080/00275514.2025.2483634","url":null,"abstract":"<p><p>Many morning glories (family Convolvulaceae) contain ergot alkaloids-important bioactive compounds produced exclusively by fungi. The ergot alkaloids of the few investigated morning glories are associated with the presence of a symbiotic Clavicipitaceous fungus. The genus <i>Periglandula</i> (Clavicipitaceae) was erected recently for two epibiotic species of morning glory symbionts. Biochemical and limited sequence data indicate that <i>Ipomoea tricolor</i>, a commonly cultivated morning glory from Mexico, contains a <i>Periglandula</i> species, but no signs of the fungus have ever been detected. Our goal was to isolate and describe this fungus, which we hypothesize represents a new species. Observation of fungal hyphae in evacuated seed coats of <i>I. tricolor</i> and subsequent transfer onto malt extract agar resulted in cultures of the symbiont isolated from the plant. The fungus grew slowly as white hyphae and sometimes aggregated into synnema-like structures, both of which lacked spores. We isolated sufficient DNA to sequence the genome with Illumina technology. Phylogenetic analyses based on multiple genes indicated that the symbiont of <i>I. tricolor</i> was distinct from, but related to, the two described species of <i>Periglandula</i> previously observed in other species of morning glories. Using quantitative polymerase chain reaction (qPCR), the fungus was quantified most abundantly in hypocotyls of <i>I. tricolor</i>, with lesser quantities in stems, cotyledons, and leaves. The fungus was not detected in roots, although ergot alkaloids were abundant in all tissues including roots. We conclude that the symbiotic fungus of <i>I. tricolor</i> is a distinct species of <i>Periglandula</i> and propose the name <i>Periglandula clandestina</i>, sp. nov.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"1-13"},"PeriodicalIF":2.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny and taxonomy of the genera of Erysiphaceae, part 7: Phyllactinieae.","authors":"Michael Bradshaw, Uwe Braun, Seyed Akbar Khodaparast, James K Mitchell, Uma Crouch, Jacklyn Thomas, Hamideh Darsaraei, Donald H Pfister","doi":"10.1080/00275514.2025.2476375","DOIUrl":"https://doi.org/10.1080/00275514.2025.2476375","url":null,"abstract":"<p><p>The seventh part of this series devoted to the phylogeny and taxonomy of powdery mildews presents the phylogeny and taxonomy of species assigned to the genera <i>Leveillula, Phyllactinia, Pleochaeta</i>, and <i>Queirozia</i> (Erysiphaceae tribe Phyllactinieae). Phylogenetic trees based on multiple loci (ITS+28S, <i>CAM, GADPH, GS</i>, IGS, <i>RPB2</i>, and <i>TUB</i>) are presented. All species with available sequence data are included. The present analyses aim at providing a reference for analyses of powdery mildews of tribe Phyllactinieae. A special focus is on the generic distinction at the base of tribe Phyllactinieae and between <i>Leveillula</i> and <i>Phyllactinia</i>. The inclusion of a larger number of phylogenetically basal <i>Phyllactinia</i> spp. and multiple loci allowed a better insight into the phylogeny of tribe Phyllactinieae and confirmed that <i>Leveillula</i> spp. are nested within the <i>Phyllactinia</i> clade. As a consequence, <i>Leveillula</i> is reduced to synonymy with <i>Phyllactinia</i>. Additionally, <i>Queirozia</i> was found to be nested within the <i>Pleochaeta</i> clade, and as such we have reduced <i>Queirozia</i> to synonymy with <i>Pleochaeta</i>. Multiple species have been sequenced for the first time, in particular multiple North American species. <i>Erysiphe betulae, E. orbicularis, E. polychaeta, E. saxaouli, Phyllactinia antarctica, Ph. broussonetiae-kaempferi, Ph. hamamelidis, Ph. kakicola, Ph. pyri-serotinae, Ph. salmonii, Ph. zanthoxylicola, Uncinula lynchii</i>, and <i>U. shiraiana</i> are epitypified. The new species <i>Ph. amelanchieris</i>, sp. nov. <i>Ph. fraxini-pennsylvanicae</i>, sp. nov. and <i>Ph. oemleriae</i>, sp. nov. are described, and the new combinations <i>Phyllactinia buddlejae, Ph. cleomes, Ph. cylindrospora, Ph. farinosa, Ph. golovinii, Ph. guilanensis, Ph. jaczewskii, Ph. lactucae-seriolae, Ph. lactucarum, Ph. lanugiosa, Ph. lappae, Ph. mindii, Ph. oxalidicola, Ph. picridis, Ph. rubiae, Ph. saxaoulii, Ph. taurica, Ph. thevenotiae, Ph. ulmi</i> (comb. et stat. nov.), <i>Ph. verbasci, Ph. wissadulae</i>, and <i>Pleochaeta erysiphoides</i> are introduced. Furthermore, <i>Phyllactinia</i> sect. <i>Basiphyllactinia</i>, sect. nov. <i>Phyllactinia</i> sect. <i>Leveillula</i>, comb. et stat. nov. and <i>Pleochaeta</i> sect. <i>Ovulariopsis</i>, comb. et stat. nov. are presented.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"1-61"},"PeriodicalIF":2.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aquatic <i>Xylaria</i>: an exotic fungus introduced into the United States on aquarium decorative wood.","authors":"Robert A Blanchette, Nickolas N Rajtar, Henry Yandrasits, Kira Cassidy Stephens, Benjamin W Held","doi":"10.1080/00275514.2025.2451522","DOIUrl":"10.1080/00275514.2025.2451522","url":null,"abstract":"<p><p>This study examined fungi with <i>Xylaria</i>-like morphology on submerged decorative wood in freshwater aquariums in Minnesota and Colorado. The wood was sold in retail stores in the United States but originated from Asia. The submerged wood had black stromatic melanized structures with white tips that grew out from the wood. As colonization progressed, the fungus produced more melanized structures along the entire length of the wood and moved to new wood placed in the aquariums. Cut segments from the fungal structures and from the colonized wood were cultured in malt extract agar supplemented with antibiotics. Pure cultures obtained were used for DNA extraction, and polymerase chain reaction (PCR) amplification was performed using ITS1F-ITS4. Sequences were compared against the National Center for Biotechnology Information (NCBI) nucleotide database using BLASTn. Isolates from the fungal structures and wood obtained from the Minnesota and Colorado aquariums were all found to be <i>Xylaria apoda</i>, a fungus not previously reported from the United States. Phylogenetic analysis shows that the aquarium isolates of <i>Xylaria</i> cluster within a well-supported clade of <i>Xylaria apoda</i>. These <i>Xylaria</i> had grown in a completely aquatic habitat, producing fruiting body-like structures for several years in the freshwater aquariums. Asexual spores were not observed but may have been disseminated into the water as they are formed. Asci and ascospores were also not observed. <i>Xylaria apoda</i> has been reported only from Asia, and it is usually found in terrestrial habitats. This report adds to our knowledge of <i>Xylaria</i> that can grow in a completely underwater environment and focuses attention on an avenue for exotic fungi to be brought into new countries where they are not native. These results also contribute to the growing body of evidence that <i>X. apoda</i> is an ecologically versatile species, capable of thriving in diverse environments, including artificial habitats such as freshwater aquariums.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"213-221"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatty acid methyl ester (FAME) profiling for species-specific characterization and detection of fungal pathogens that cause tree and grapevine trunk diseases.","authors":"Christopher M Wallis, Kendra Baumgartner","doi":"10.1080/00275514.2024.2439753","DOIUrl":"10.1080/00275514.2024.2439753","url":null,"abstract":"<p><p>Fungal trunk diseases are of major concern for tree fruit, nut, and grape growers throughout the world. These diseases include Eutypa dieback of grape, caused by <i>Eutypa lata</i>, band canker of almond, caused by <i>Neofusicoccum mediterraneum</i> and <i>Neofusicoccum parvum</i>, and twig and branch dieback of walnut, caused by <i>N. mediterraneum</i>, Botryosphaeria dieback of grape, caused by <i>Diplodia mutila, Diplodia seriata, N. mediterraneum</i>, and <i>N. parvum</i>, and esca of grape, caused by <i>Phaeomoniella chlamydospora</i> and <i>Phaeoacremonium minimum</i>. Given the common occurrence of mixed infections, and the similar wood symptoms at the macroscopic level, species-specific detection tools are needed. Fatty acid methyl ester (FAME) profiling can be an effective and inexpensive diagnostic tool. FAME analyses were conducted on pure cultures of multiple isolates per species to characterize profiles and assess whether this technique could result in consistent identification. FAME profiles were dominated by oleic acid (18:1 ω9c) and palmitic acid (16:0), with less abundant FAMEs in different ratios for each species and isolates within species. Canonical discriminant analyses revealed which minor FAMEs were most variable, with a total of 20 different FAMEs that can explain 69.01% of profile variance in the first two canonicals. Using these analyses, samples were self-tested and correctly sorted 97.18% of the time. Within species, canonical discriminant analyses were able to separate isolates further, often by original geographic location or by host plant species. These results further suggest that potential novel species, subspecies, or races may be present among the isolates analyzed, demonstrating the capacity of FAME profiling to have a role in discovering cryptic species and accurately identifying fungal pathogens in conjunction with other molecular techniques and genomic analyses.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"319-330"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ubiquity and diversity of <i>Basidiobolus</i> across amphibian species inhabiting an urbanization gradient.","authors":"Madison R Hincher, Jasper P Carleton, Sara J Wheeler, Manning DelCogliano, Kaitlyn Mathis, Javier F Tabima","doi":"10.1080/00275514.2025.2455909","DOIUrl":"10.1080/00275514.2025.2455909","url":null,"abstract":"<p><p>The role of microfungal species in the environment is wide and well documented, especially in terms of symbiosis. Nonetheless, microfungal species are usually overseen and vastly understudied. One example of these understudied microfungal groups is the genus <i>Basidiobolus</i>, an ecologically diverse zoopagomycete genus found within vertebrate gastrointestinal systems, a saprobe across leaf litter, or as an opportunistic pathogen of immunocompromised humans. Studies of <i>Basidiobolus</i> diversity and distribution have been focused mostly on non-urbanized areas of subtropical regions, but there is a recent paucity of studies on this genus in temperate and densely human populated areas. Here, we present insights into the ubiquity and diversity of <i>Basidiobolus</i> species associated with amphibian species that live in the Worcester waterway system, a system of connecting streams and ponds that originate in pristine, protected wild management areas, and the highly urbanized downtown area of Worcester, Massachusetts. Our results show the ubiquitous presence of <i>Basidiobolus</i> across the gastrointestinal tract samples of amphibians spanning diverse species and habitats, including conservation areas, urban watersheds, and rural ecosystems. Our study reveals that multiple individuals and species of <i>Basidiobolus</i> coexist within a single host, suggesting complex interactions within amphibian gut microbiomes. Finally, we present possible novel diversity in the genus, indicating that further studies should be focused on understanding the species richness, genetic diversity, and ecological roles and associations of this interesting fungal group.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"222-234"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}