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Rediscovery of the tardigrade pathogen Sorochytrium milnesiophthora, a blastoclad boasting the longest-known fungal internal transcribed spacer. 重新发现缓步动物病原体千疫霉,一种具有已知时间最长的真菌内部转录间隔的胚包膜。
IF 2.6 2区 生物学
Mycologia Pub Date : 2026-05-01 Epub Date: 2026-03-09 DOI: 10.1080/00275514.2025.2611368
Alden C Dirks, Matteo Vecchi, Michelle Orozco-Quime, Eva Schwarz, Sara Calhim, Timothy Y James
{"title":"Rediscovery of the tardigrade pathogen <i>Sorochytrium milnesiophthora</i>, a blastoclad boasting the longest-known fungal internal transcribed spacer.","authors":"Alden C Dirks, Matteo Vecchi, Michelle Orozco-Quime, Eva Schwarz, Sara Calhim, Timothy Y James","doi":"10.1080/00275514.2025.2611368","DOIUrl":"10.1080/00275514.2025.2611368","url":null,"abstract":"<p><p><i>Sorochytrium milnesiophthora</i> is a necrotrophic pathogen of tardigrades and a member of the poorly sampled zoosporic phylum Blastocladiomycota. First described in 1985, it was previously known only from the type locality, the Appalachian Mountains of North Carolina, USA, and had not been documented in over 35 years. We rediscovered <i>S. milnesiophthora</i> in a lichen sample from Finland, cultured the fungus, and sequenced its genome. Ribosomal DNA and phylogenomic analyses placed <i>S. milnesiophthora</i> in the Blastocladiales as sister to the rest of the order. The Sorochytriaceae clade also included environmental sequences from Brazil, Italy, Poland, and Sweden, indicating a wide distribution and substantial unexplored biodiversity of <i>Sorochytrium</i>-like fungi. To our surprise, <i>S. milnesiophthora</i> was found to have an exceptionally large nuclear rDNA ITS1-5.8S-ITS2 (internal transcribed spacer [ITS]) region of approximately 5500 bp, the largest known in Fungi. This extraordinary ITS length may represent an adaptation to desiccation by slowing ribosome biogenesis. Further studies should examine how <i>S. milnesiophthora</i> infection intersects with tardigrade cryptobiosis and whether the fungus can also survive the extreme conditions for which its host is famous. Deposition of <i>S. milnesiophthora</i> in the Collection of Zoosporic Eufungi at University of Michigan ensures its long-term availability for future investigations.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"623-637"},"PeriodicalIF":2.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147390506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Filamentous fungal communities associated with the eastern larch beetle (Dendroctonus simplex LeConte) in Minnesota, including new Ophiostomatales species. 与明尼苏达州东部落叶松甲虫(Dendroctonus simplex LeConte)相关的丝状真菌群落,包括新的Ophiostomatales物种。
IF 2.6 2区 生物学
Mycologia Pub Date : 2026-05-01 Epub Date: 2026-02-26 DOI: 10.1080/00275514.2025.2609488
Andrew J Mann, Amelia G Lochridge, Rin M Barnum, Jennifer Galarneau, Eric C Otto, Nickolas N Rajtar, Benjamin W Held, Kathryn E Bushley, Robert A Blanchette
{"title":"Filamentous fungal communities associated with the eastern larch beetle (<i>Dendroctonus simplex</i> LeConte) in Minnesota, including new Ophiostomatales species.","authors":"Andrew J Mann, Amelia G Lochridge, Rin M Barnum, Jennifer Galarneau, Eric C Otto, Nickolas N Rajtar, Benjamin W Held, Kathryn E Bushley, Robert A Blanchette","doi":"10.1080/00275514.2025.2609488","DOIUrl":"10.1080/00275514.2025.2609488","url":null,"abstract":"<p><p>The eastern larch beetle (<i>Dendroctonus simplex</i> LeConte) is a native and historically nonaggressive inhabitant of <i>Larix laricina</i> trees in North America. Since 2001, however, <i>D. simplex</i> has experienced an uncharacteristic and prolonged outbreak that has impacted trees on approximately 90% of the <i>L. laricina</i> forests in Minnesota. To investigate the fungal communities within <i>D. simplex</i> galleries during this outbreak, we isolated fungi from 56 <i>D. simplex</i>-infested trees across seven sites in Minnesota. We recovered 45 fungal species, including four new species in the order Ophiostomatales, proposed as <i>Graphilbum insulare</i>, sp. nov., <i>Ophiostoma itasca</i>, sp. nov., <i>Ophiostoma minnesotense</i>, sp. nov., and <i>Ophiostoma pseudoips</i>, sp. nov. Additionally, based on phylogenetics and morphological traits, we recommend a new taxonomic classification for <i>Leptographium simplex</i>, comb. nov. previously described as <i>Graphium simplex</i> from <i>D. simplex</i> galleries in Vermont, USA. At the community level, most isolates were in the phylum Ascomycota (82% of the total relative abundance), followed by Basidiomycota (12%). The most frequently isolated orders were Ophiostomatales (59% of the total relative abundance), Hypocreales (9%), and Pleosporales (8%). One species, <i>Grosmannia americana</i>, accounted for more than half of all isolates recovered. These results improve our understanding of the fungi associated with a bark beetle outbreak and an understudied tree species.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"457-469"},"PeriodicalIF":2.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147307821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rust fungi (Pucciniales, Basidiomycota) of the Brazilian Cerrado: Taxonomic advances and new taxa in a threatened biome. 巴西塞拉多地区的锈菌(锈菌门,担子菌门):一个受威胁生物群的分类进展和新分类群。
IF 2.6 2区 生物学
Mycologia Pub Date : 2026-05-01 Epub Date: 2026-03-11 DOI: 10.1080/00275514.2025.2607811
Malte Ebinghaus, João M T Martins, Maria D M Santos, Dirceu Macagnan, Silvino Intra Moreira, Erica S C Souza, Zuleide M Chaves, Denise V de Rezende, José R Lucas Gomes, Anibal A Carvalho-Junior, Danilo B Pinho, José C Dianese
{"title":"Rust fungi (Pucciniales, Basidiomycota) of the Brazilian Cerrado: Taxonomic advances and new taxa in a threatened biome.","authors":"Malte Ebinghaus, João M T Martins, Maria D M Santos, Dirceu Macagnan, Silvino Intra Moreira, Erica S C Souza, Zuleide M Chaves, Denise V de Rezende, José R Lucas Gomes, Anibal A Carvalho-Junior, Danilo B Pinho, José C Dianese","doi":"10.1080/00275514.2025.2607811","DOIUrl":"10.1080/00275514.2025.2607811","url":null,"abstract":"<p><p>The Brazilian savanna designated as Cerrado is one of the most biodiverse biomes in the world, yet it has lost nearly 50% of its native vegetation since the mid-20th century, mostly due to agricultural expansion. This rapid degradation makes it one of the most threatened biodiversity hot spots worldwide, highlighting the urgent need for intensified conservation and biodiversity research efforts. The systematic study of rust fungi (Pucciniales) in the Brazilian Cerrado began around 140 years ago with the temporary emigration from Germany of the explorer and collector Ernst Heinrich Georg Ule (*1854-†1915) to Brazil where he was hired as a \"visiting naturalist\" serving in the National Museum of Rio de Janeiro from 1891 until 1895. Since then, approximately 270 species of rust fungi have been documented in the Cerrado. Historically, taxonomic classifications of rust fungi relied strongly on the interpretation of morphological traits, which are prone to subjective bias that has led to taxonomic instability and is reflected in complex taxonomic histories. Furthermore, recent molecular phylogenetic studies have revealed a high frequency of homoplasious traits in rust fungal morphology, further complicating accurate taxonomic decisions when such traits are considered in isolation. In this study, we conducted morphological and molecular phylogenetic analyses on several rarely collected and studied genera of Cerrado rust fungi, evaluating phylogenetic relationships and discussing their taxonomy. We describe a new <i>Puccinia</i> species infecting the genus <i>Coracoralina</i> (Eriocaulaceae: Poales), <i>Puccinia coracoralinae</i>, sp. nov. along with a new genus <i>Dietelomyces</i>, gen. nov. and several new combinations, i.e. <i>Cerradopsora pouteriae</i>, nom. nov. <i>Dietelomyces copaiferae</i>, comb. nov. besides defining the suprageneric status of <i>Esalque holwayi, Dietelia duguetiae, Kimuramyces cerradensis</i>, and <i>Mimema venturae</i>. Additionally, we discuss the effects of identified homoplasious traits on rust fungal systematics based on our phylogenetic analyses. Considering published estimates of rust fungal diversity in other regions, we conclude that with approximately 12 356 documented vascular plant species in the Cerrado, at least ca. 2300 rust fungal species can be expected to be present. This suggests that, to date, ca. of 13% of the rust fungi occurring in the Cerrado has been documented. Given the alarming threat status of the Cerrado and its extremely significant biodiversity, we also emphasize and discuss the potential implications of systematic rust fungal research for future conservation policies in this unique ecosystem. By addressing key taxonomic and phylogenetic gaps, this study highlights rust fungi as a critically understudied component of Cerrado biodiversity and reinforces the urgent need to expand field collections and integrate fungal systematics with conservation strategies as habitat loss accelerates.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"583-622"},"PeriodicalIF":2.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147434279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unraveling the genomic and phylogenetic complexity of the under-studied microfungus Basidiobolus: Insights from 19 newly sequenced genomes. 揭示未被充分研究的担子孢子微真菌的基因组和系统发育复杂性:来自19个新测序基因组的见解。
IF 2.6 2区 生物学
Mycologia Pub Date : 2026-05-01 Epub Date: 2026-03-13 DOI: 10.1080/00275514.2026.2619989
Jasper P Carleton, Alexander J Bradshaw, Liam P Cleary, Madison R Hincher, Kathryn Bushley, Javier F Tabima
{"title":"Unraveling the genomic and phylogenetic complexity of the under-studied microfungus <i>Basidiobolus</i>: Insights from 19 newly sequenced genomes.","authors":"Jasper P Carleton, Alexander J Bradshaw, Liam P Cleary, Madison R Hincher, Kathryn Bushley, Javier F Tabima","doi":"10.1080/00275514.2026.2619989","DOIUrl":"10.1080/00275514.2026.2619989","url":null,"abstract":"<p><p><i>Basidiobolus</i> is a globally distributed genus of non-Dikarya fungi within Zoopagomycota, known for its presence in diverse ecological niches ranging from soil and decaying organic matter to vertebrate gastrointestinal tracts. Despite its ecological and medical relevance, the taxonomy and evolutionary relationships within the genus remain poorly resolved due to limited genomic resources. In this study, we present 19 newly sequenced <i>Basidiobolus</i> genomes, expanding the available genomic data. Using short-read Illumina sequencing, assembly, and annotation pipelines, we characterize gene content, assess completeness, and explore biosynthetic gene content across isolates, one with 14 genomes that includes <i>B. ranarum</i> and another with eight genomes that includes <i>B. meristosporu</i>s; a single genome of <i>B. heterosporus</i> forms a distinct lineage. Several isolate groups exhibit deep divergence suggestive of novel species, underscoring the need for expanded sampling and taxonomic revision. Functional annotations reveal a rich repertoire of biosynthetic gene clusters, including non-ribosomal peptide synthetases, polyketide synthases, and hybrid clusters, pointing to an underexplored reservoir of secondary metabolite diversity. These findings position <i>Basidiobolus</i> as a compelling model for investigating fungal evolution, ecological adaptation, and natural product biosynthesis.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"482-495"},"PeriodicalIF":2.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147444455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fire history and frequency alter arbuscular mycorrhizal fungal traits, community dynamics, and symbioses. 火灾历史和频率改变丛枝菌根真菌的性状、群落动态和共生关系。
IF 2.6 2区 生物学
Mycologia Pub Date : 2026-05-01 Epub Date: 2026-02-27 DOI: 10.1080/00275514.2026.2616218
Jacob R Hopkins, Kristian Harris, Alison E Bennett
{"title":"Fire history and frequency alter arbuscular mycorrhizal fungal traits, community dynamics, and symbioses.","authors":"Jacob R Hopkins, Kristian Harris, Alison E Bennett","doi":"10.1080/00275514.2026.2616218","DOIUrl":"10.1080/00275514.2026.2616218","url":null,"abstract":"<p><p>Fire is a common disturbance that structures terrestrial ecosystems. Since climate change models predict increases in fire frequency, it is important to study how changes in fire regime influence plant fuel-related above- and belowground ecosystem components. Due to their role in plant fuel production, arbuscular mycorrhizal (AM) fungal spore communities and symbioses with plants are invaluable for understanding how changes in fire regime influence belowground communities and alter fuel-related above- and belowground processes such as plant growth. With a greenhouse experiment, we tested how variation in fire frequency and history influenced AM fungal community composition, traits, and their interactions with host plants by manipulating fire regimes over three successive generations of AM fungi and <i>Rudbeckia hirta</i> plants. We found that fire frequency effects on spore traits such as pigmentation and sporulation mediated changes in AM fungal spore communities, but these effects took time to develop and were associated with reduced plant growth. This demonstrates that increases in fire frequency can alter the fire-fuel feedbacks that underlie pyrophilic ecosystems.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"470-481"},"PeriodicalIF":2.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147317721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monomethylhydrazine content is determined by altitude in Gyromitra antarctica: Implications for safe consumption. 南极洲Gyromitra的单甲基肼含量由海拔决定:对安全消费的影响。
IF 2.6 2区 生物学
Mycologia Pub Date : 2026-04-30 DOI: 10.1080/00275514.2026.2641837
Romina B Parada, Roberto Garibay Orijel, Andrés de Errasti, María Belén Pildain, Carolina Barroetaveña
{"title":"Monomethylhydrazine content is determined by altitude in <i>Gyromitra antarctica</i>: Implications for safe consumption.","authors":"Romina B Parada, Roberto Garibay Orijel, Andrés de Errasti, María Belén Pildain, Carolina Barroetaveña","doi":"10.1080/00275514.2026.2641837","DOIUrl":"https://doi.org/10.1080/00275514.2026.2641837","url":null,"abstract":"<p><p>Several species of the genus <i>Gyromitra</i>-commonly known as \"false morels\"-are traditionally consumed in different parts of the world, despite their potential toxicity due to the presence of monomethylhydrazine, a volatile compound. In some taxa, monomethylhydrazine content has been linked to environmental factors such as elevation, potentially influencing toxicity levels. In southern South America, ethnomycological records indicate that <i>Gyromitra</i> species are collected and consumed by indigenous communities. However, their taxonomy and toxicological profile remain poorly understood. In this study, we examined the phylogenetic identity and hydrazine content of <i>Gyromitra</i> specimens collected across a Patagonian elevation gradient. Molecular analyses based on nuclear internal transcribed spacer, large subunit, and elongation factor-1 alpha sequences confirmed the identity of all specimens as <i>G. antarctica</i>. Hydrazine content was quantified in 82 fresh samples collected between 300 and 800 m above sea level, revealing a significant negative correlation between elevation and toxin concentration (ρ = -0.79, <i>P</i> < 0.001). Two boiling treatments -10 min without water exchange, and 2 × 5 min with water exchange-reduced hydrazine levels by 97% and 94%, respectively. However, residual toxins were detected in the boiling water of both treatments. These findings provide new insights into the taxonomy and elevation-dependent toxicity of <i>Gyromitra</i> in the Southern Hemisphere. They also underscore the risks associated with the consumption of wild false morels and highlight the importance of informed and adequate culinary practices to reduce toxicity.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"1-13"},"PeriodicalIF":2.6,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147817638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Defined polyxenic cultivation of species of Didymium and Physarum for small-scale laboratory production of plasmodia, sclerotia, and sporocarps. 为小规模实验室生产疟原虫、菌核菌和孢子囊而确定的双孢菌和绒泡菌的多种培养。
IF 2.6 2区 生物学
Mycologia Pub Date : 2026-04-15 DOI: 10.1080/00275514.2026.2641983
Johann Gangl, Gabriela Nagl-Neuhold, Myriam de Haan, Marc Lemmens, Rudolf Krska
{"title":"Defined polyxenic cultivation of species of <i>Didymium</i> and <i>Physarum</i> for small-scale laboratory production of plasmodia, sclerotia, and sporocarps.","authors":"Johann Gangl, Gabriela Nagl-Neuhold, Myriam de Haan, Marc Lemmens, Rudolf Krska","doi":"10.1080/00275514.2026.2641983","DOIUrl":"https://doi.org/10.1080/00275514.2026.2641983","url":null,"abstract":"<p><p>Myxomycetes (plasmodial slime molds or myxogastrids) have long attracted scientific attention for their complex life cycles and unique behaviors, yet they remain underexplored in applied research contexts such as plant pathology. This study established reproducible, stepwise protocols for the cultivation, maintenance, and induction of sclerotia and sporocarps under defined polyxenic conditions of four species of myxomycetes, <i>Didymium squamulosum, D. bahiense, Physarum cinereum</i>, and <i>P. compressum</i> originally collected from maize. Starting from spore-based cultures, we optimized feeding regimes using bacterial and yeast mixtures, introduced a purification method combining antibiotic agar and low-heat-killed yeast, and demonstrated long-term culture maintenance at low temperature. Growth dynamics were modeled via the area of microbial feed consumed, revealing strong species- and temperature-dependent differences. Controlled sclerotization yielded sclerotia with quantifiable macrocyst activity, assessed by phase-contrast microscopy of cytoplasmic movement as a reproducible proxy for macrocyst functionality. Sporulation was successfully induced for all four species, with interspecific variation in sporocarp formation and spore germination rates. Together, these methods enable full life cycle completion in the laboratory and provide standardized inoculum sources in small scale for downstream applications.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"1-17"},"PeriodicalIF":2.6,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147691034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The genus Phaeocollybia from southwestern China based on morphological and molecular evidence. 基于形态学和分子证据的中国西南褐藻属研究。
IF 2.6 2区 生物学
Mycologia Pub Date : 2026-04-07 DOI: 10.1080/00275514.2026.2626246
San-Zhao Chen, Yun-Hong Tan, Tai-Wen Huang, Dong-Yi Liang, Jun-Gao Chen, Zhu L Yang, Yan-Chun Li
{"title":"The genus <i>Phaeocollybia</i> from southwestern China based on morphological and molecular evidence.","authors":"San-Zhao Chen, Yun-Hong Tan, Tai-Wen Huang, Dong-Yi Liang, Jun-Gao Chen, Zhu L Yang, Yan-Chun Li","doi":"10.1080/00275514.2026.2626246","DOIUrl":"https://doi.org/10.1080/00275514.2026.2626246","url":null,"abstract":"<p><p>Four new species of the genus <i>Phaeocollybia</i> (Hymenogastraceae, Agaricales) from Yunnan Province, southwestern China, namely, <i>P. ochrilata, P. conicogregaria, P. profundirhizoidea</i>, and <i>P. purpureorosea</i>, are described and illustrated based on morphological data and multilocus phylogenetic analyses from DNA sequences of the internal transcribed spacer (ITS) and the second largest subunit of RNA polymerase II (<i>RPB2</i>). Our phylogenetic analyses revealed that the four new species belong to <i>P</i>. subgen. <i>Phaeocollybia</i>. Color photos of fresh basidiomata, line drawings of microscopic features, and their comparisons with allied taxa are presented.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"1-18"},"PeriodicalIF":2.6,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of bidirectional solid fermentation of Irpex lacteus and Chinese herbal medicines on alterations in antifungal activity. 乳酸菌与中草药双向固体发酵对其抗真菌活性的影响。
IF 2.6 2区 生物学
Mycologia Pub Date : 2026-03-31 DOI: 10.1080/00275514.2026.2630619
Chengxin Huang, Huiyue Hu, Ying Zhang, Wang Shu, Yongxun Duan, Yueting Dai, Lingyun Liu, Changtian Li
{"title":"The effect of bidirectional solid fermentation of <i>Irpex lacteus</i> and Chinese herbal medicines on alterations in antifungal activity.","authors":"Chengxin Huang, Huiyue Hu, Ying Zhang, Wang Shu, Yongxun Duan, Yueting Dai, Lingyun Liu, Changtian Li","doi":"10.1080/00275514.2026.2630619","DOIUrl":"https://doi.org/10.1080/00275514.2026.2630619","url":null,"abstract":"<p><p>Bidirectional solid fermentation can augment the therapeutic efficacy of Chinese herbal medicines by increasing their bioactive constituents. Chinese herbal medicines demonstrate specific antifungal properties. The potential of bidirectional solid fermentation to augment the antifungal efficacy of Chinese herbal treatments against plant-pathogenic fungi remains unexamined. This study employed bidirectional solid fermentation utilizing <i>Irpex lacteus</i> and eight traditional Chinese herbal medicines to investigate alterations in antifungal efficacy. The findings indicated that <i>I. lacteus</i> had comparatively fast development on the majority of Chinese herbal medicine substrates. Bidirectional solid fermentation markedly improved antifungal efficacy across the majority of substrates. The ethanol extract of <i>Origanum vulgare</i>, fermented for 10 d, exhibited an inhibition rate against most plant-pathogenic fungi equivalent to that of carbendazim at the same dose. The correlation analysis indicated a favorable association between total terpenoid concentration and antifungal efficacy. The metabolomic investigation indicated that, relative to the unfermented control, the metabolites considerably elevated in <i>Origanum vulgare</i> after 10 d of fermentation were primarily terpenoids, including pseudolaric acid B and prednisolone acetate, whereas the significantly decreased metabolites were primarily shikimic acid and phenylpropanoids, such as salvianolic acid C and icariside I. The KEGG (Kyoto Encyclopedia of Genes and Genomes)  pathway enrichment analyses revealed that the most significantly enriched pathway was tyrosine metabolism. These findings will help facilitate the creation of prospective biocontrol agents, therefore offering sustainable alternatives for crop disease management.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"1-13"},"PeriodicalIF":2.6,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phylogeny and diversity of Rhizopogon species in Japan. 日本根瘤菌的系统发育与物种多样性。
IF 2.6 2区 生物学
Mycologia Pub Date : 2026-03-30 DOI: 10.1080/00275514.2026.2620129
Hiroshi Abe, Hiromi Sasaki, Kazuhide Nara
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