MycologiaPub Date : 2025-09-01Epub Date: 2025-07-21DOI: 10.1080/00275514.2025.2516968
Tatiana Yu Gagkaeva, Aleksandra S Orina, Olga P Gavrilova
{"title":"<i>Fusarium neoglobosum</i>, a novel species within the Asian clade of <i>Fusarium fujikuroi</i> complex.","authors":"Tatiana Yu Gagkaeva, Aleksandra S Orina, Olga P Gavrilova","doi":"10.1080/00275514.2025.2516968","DOIUrl":"10.1080/00275514.2025.2516968","url":null,"abstract":"<p><p>From 2017 to 2024, 10 strains morphologically similar to <i>Fusarium globosum</i> were obtained from cereals in West Siberia, the Urals, and the Central European region of Russia. This study was conducted to confirm the species identity of the strains morphologically assigned to <i>F</i>. cf. <i>globosum</i>. For this purpose, comparison with <i>F. globosum</i> strain KSU 11554 from South Africa was performed using multilocus analysis, morphological features, growth tests, and production of secondary metabolites. In order to determine phylogenetic relationships, the translation elongation factor 1α, β-tubulin, RNA polymerase II second largest subunit, and histone H3 genes were used. Strains from Japan and Russia formed a separate and well-supported lineage, representing a single, previously undescribed species, described here as <i>F. neoglobosum</i>. Both <i>F. globosum</i> and <i>F. neoglobosum</i> are deeply nested within the Asian clade in the molecular phylogeny of <i>Fusarium fujikuroi</i> species complex. The key character in both species is a formation of globose microconidia in the dark. Opposite mating type idiomorphs was detected in <i>F. globosum</i> and <i>F. neoglobosum</i>, which is indicative of the heterothallic nature of sexual reproduction. Strains described as <i>F. neoglobosum</i> exhibit two mycotoxin-related phenotypes: mycotoxin-producing and non-mycotoxin-producing. Five <i>F. neoglobosum</i> strains and a <i>F. globosum</i> strain were capable of producing a wide range of fumonisins. None of the strains of either species produced moniliformin, and only 2 of 10 <i>F. neoglobosum</i> strains produced detectable beauvericin. The data presented confirm the distribution of <i>F. neoglobosum</i> in East and North Asia, with only one strain isolated from European Russia.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"965-982"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2025-09-01Epub Date: 2025-06-26DOI: 10.1080/00275514.2025.2516372
Min Liu, Zengqing Su, Shiyu Zhang, Zhuo Fan, Peiyi Zhao, Xiaoping Xing, Wei Zhao, Honglian Li, Linlin Chen
{"title":"A secreted glycerophosphodiester phosphodiesterase (GDPD) domain-containing protein, FpGDPD, is involved in <i>Fusarium pseudograminearum</i> virulence.","authors":"Min Liu, Zengqing Su, Shiyu Zhang, Zhuo Fan, Peiyi Zhao, Xiaoping Xing, Wei Zhao, Honglian Li, Linlin Chen","doi":"10.1080/00275514.2025.2516372","DOIUrl":"10.1080/00275514.2025.2516372","url":null,"abstract":"<p><p>Microbial pathogens secrete a diverse array of proteins to manipulate plant defense mechanisms and facilitate infection. However, few secreted proteins were thoroughly characterized in <i>Fusarium pseudograminearum</i>. Here, we identify and characterize <i>FpGDPD</i>, a gene encoding a secretory glycerophosphodiester phosphodiesterase (GDPD) domain-containing protein in <i>F. pseudograminearum</i>. We found that <i>FpGDPD</i> expression was significantly upregulated during specific stages of fungal infection. Disruption of <i>FpGDPD</i> in <i>F. pseudograminearum</i> did not affect vegetative growth, stress responses, or conidiation but enhanced phosphate tolerance and reduced fungal virulence in wheat. Furthermore, we demonstrated the secretory nature of the N-terminal signal peptide of FpGDPD and that the signal peptide contributes to <i>F. pseudograminearum</i> pathogenicity. Interestingly, FpGDPD triggers immune responses in <i>Nicotiana benthamiana</i>, inducing reactive oxygen species (ROS) accumulation and upregulating defense-related genes. Collectively, our findings reveal that FpGDPD plays a dual role: it promotes fungal virulence while simultaneously eliciting plant immune responses.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"925-936"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144497508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2025-09-01Epub Date: 2025-07-11DOI: 10.1080/00275514.2025.2518029
Zi-Rui Wang, Hong Yu, Zhu L Yang
{"title":"<i>Cystinarius</i>: phylogeny and species diversity in China.","authors":"Zi-Rui Wang, Hong Yu, Zhu L Yang","doi":"10.1080/00275514.2025.2518029","DOIUrl":"10.1080/00275514.2025.2518029","url":null,"abstract":"<p><p><i>Cystinarius</i> is a small genus belonging to the family Cortinariaceae, distinguished by its distinctive combination of small basidiospores and presence of both cheilo- and pleurocystidia, a combination that is rarely found in other genera within the Cortinariaceae. To date, this genus consists of only two subgenera and includes seven recognized species. Previously, only a single specimen of <i>Cy. crassus</i> was reported from China. Based on the analysis of 17 newly collected specimens, supported by morphological evidence and two-locus phylogenetic inference, five species, namely, <i>Cy. fulvellus, Cy. longipes, Cy. shennongensis, Cy. crassus</i>, and <i>Cy. paurigarhwalensis</i>, were recognized. Among them, the former three species are new to science, whereas the last one is new to China. This study provides comprehensive taxonomic descriptions and a detailed identification key to the <i>Cystinarius</i> species discovered in China.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"983-999"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2025-09-01Epub Date: 2025-07-11DOI: 10.1080/00275514.2025.2513710
David Pelissero, Francisco Kuhar, Gabriel Grilli, Joszef Geml, Matthew E Smith, Esteban M Crespo, Maria L Hernandez Caffot, Eduardo Nouhra
{"title":"Unveiling the cryptic Neotropical species of <i>Phaeoclavulina</i> from the Southern Cone: phylogenetic and morphological analysis.","authors":"David Pelissero, Francisco Kuhar, Gabriel Grilli, Joszef Geml, Matthew E Smith, Esteban M Crespo, Maria L Hernandez Caffot, Eduardo Nouhra","doi":"10.1080/00275514.2025.2513710","DOIUrl":"10.1080/00275514.2025.2513710","url":null,"abstract":"<p><p>Global fungal diversity is estimated at about 6.2 million species, but only 150 000 are currently described. Molecular studies reveal that this diversity is often underestimated, especially in less-studied regions. <i>Phaeoclavulina</i>, a genus of ramarioid fungi, has a wide distribution in areas with temperate and tropical climates. However, in southern South America, research on <i>Phaeoclavulina</i> species has been scarce, with only a few studies conducted in Argentina covering the morphology of a few species. This research aims to analyze <i>Phaeoclavulina</i> species in Argentina, exploring their morphology, ecological roles, and distribution. Four different ecological regions across Argentina were sampled. Molecular data (Sanger and next-generation sequencing [NGS] technology) were obtained to construct their phylogenetic relationships. Our results show a greater diversity of <i>Phaeoclavulina</i> than previously known. Eight taxa are described here, four of which are new species to science (<i>Phaeoclavulina aena, P. angularis, P. prasina</i>, and <i>P. stelligera</i>). Additionally, a neotypification is proposed for <i>Phaeoclavulina camellia</i>. For the other three species (<i>P. articulotela, P. campoi</i>, and <i>P. minutispora</i>), we provide new molecular data and elucidate their phylogenetic relationships with other previously described species. In addition, a key to species of <i>Phaeoclavulina</i> from Argentina and Chile is included to facilitate identification of known taxa. Finally, most <i>Phaeoclavulina</i> species richness is associated with tropical and subtropical forests.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"899-924"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2025-09-01Epub Date: 2025-07-15DOI: 10.1080/00275514.2025.2513195
N P Maslova, A B Sokolova, E Yu Blagoveshchenskaya, M V Tekleva
{"title":"The new genus of epiphyllous fungi on sequoioid leaves from the Albian-Cenomanian of Western Siberia, Russia.","authors":"N P Maslova, A B Sokolova, E Yu Blagoveshchenskaya, M V Tekleva","doi":"10.1080/00275514.2025.2513195","DOIUrl":"10.1080/00275514.2025.2513195","url":null,"abstract":"<p><p>Co-evolution of plants and fungi is of great importance for the phylogeny of both groups. Here we establish fossil thyriothecioid stromata on sequoioid (Cupressaceae) leaves from the Albian-Cenomanian of Western Siberia, Russia, which are assigned to the new fossil genus and species, <i>Kiyamyces sequoiae</i>. The new genus is described on the basis of thyriothecioid stromata arising from a host plant stoma, appearing first as a columella and with tendency to be confluent forming crusts. Thyriothecioid stromata are characterized by a \"basal layer,\" a scutellum of radial, septate cells, a pseudoparenchyma inside the thyriothecioid stromata; a prominent hypostroma and an endophytic, septate mycelium. The new data contribute to our understanding of the range of character combinations of early epiphyllous Ascomycota. The newly discovered fungus and the conifer plant in the Cretaceous extend the range of character combinations of early epiphyllous Dothideomycetes.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"858-883"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2025-09-01Epub Date: 2025-08-04DOI: 10.1080/00275514.2025.2516969
Marina F Bracale, José Mardgan-Neto, Débora C Guterres, Robert W Barreto
{"title":"Novel genera of Parmulariaceae on Arecaceae from Brazil.","authors":"Marina F Bracale, José Mardgan-Neto, Débora C Guterres, Robert W Barreto","doi":"10.1080/00275514.2025.2516969","DOIUrl":"10.1080/00275514.2025.2516969","url":null,"abstract":"<p><p>The ascomycetes placed in Parmulariaceae represent a diverse assemblage of biotrophic plant parasites. They usually produce discoid/scutellinioid stromata that are external to erumpent on living leaves of plants in the tropics. Disease symptoms are usually mild, and fungal structures are often cryptic on their hosts. These fungi are poorly known to science, and their classification has relied mostly on morphological features. There was a general lack of molecular data for taxa placed in this family, until recently, and morphological descriptions of many genera and species were brief and incomplete. Morphology remains the main source of information for taxonomic delimitation within the family. Here we follow a polyphasic approach, combining morphological, molecular, and host association information in order to elucidate the identity of three members of the Parmulariaceae found on samples of three neotropical palm species (Arecaceae), namely, (i) <i>Acrocomia aculeata</i> (macaw palm), (ii) <i>Astrocaryum aculeatissimum</i> (brejaúva), and (iii) <i>Syagrus romanzoffiana</i> (queen palm). Each palm species is a host for a novel species, each belonging to a new genus. The following names are proposed: (i) <i>Concertinularia acrocomiae</i>, sp. et gen. nov.-on macaw palm; (ii) <i>Amphistromularia astrocaryi</i>, sp. et gen. nov.-on brejaúva, and (iii) <i>Labyrinthularia syagri</i>, sp. et gen. nov.-on queen palm.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"937-950"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2025-09-01Epub Date: 2025-07-23DOI: 10.1080/00275514.2025.2522020
Warong Suksavate, C Ngernsaengsaruay, M Nipitwattanaphon, S Hasin, C Phosri, I Voraphab, B Sakolrak, P Choosa-Nga, L Nakpong, R Khunkrai, K Bunlerlerd, Tharnrat Kaewgrajang
{"title":"Effect of climate change on truffle (<i>Tuber</i> species) distribution and host plant interactions in Thailand.","authors":"Warong Suksavate, C Ngernsaengsaruay, M Nipitwattanaphon, S Hasin, C Phosri, I Voraphab, B Sakolrak, P Choosa-Nga, L Nakpong, R Khunkrai, K Bunlerlerd, Tharnrat Kaewgrajang","doi":"10.1080/00275514.2025.2522020","DOIUrl":"10.1080/00275514.2025.2522020","url":null,"abstract":"<p><p>Climate change is an important driver of shifts in species' geographic distributions, including those of several truffle species. Understanding these shifts is essential for effective conservation and sustainable ecosystem management. This study aimed to identify suitable habitats for three <i>Tuber</i> species discovered in Thailand-<i>Tuber lannaense, T. thailandicum</i>, and <i>T. magnatum-</i>and project their future distributions under climate change scenarios. Using MaxEnt modeling and presence-only occurrence data, we predicted current and future suitable habitats under two climate scenarios: SSP1-2.6 (low emissions) and SSP5-8.5 (high emissions) for the year 2050. Annual precipitation (bio12) and mean diurnal range (bio02) were the most influential environmental variables for three <i>Tuber</i> species and their host plants. Currently, suitable habitats for <i>Tuber</i> species and their host trees (<i>Betula alnoides</i> and <i>Carpinus londoniana</i>) are concentrated in mountainous areas of northern and northeastern Thailand, covering approximately 6000 km<sup>2</sup>. By 2050, under both SSP1-2.6 and SSP5-8.5 scenarios, the shared suitable habitat between truffles and their host plants is projected to be completely lost (100%). <i>Carpinus londoniana</i> is expected to lose nearly 100% of its suitable habitat under SSP1-2.6 and retain only 37 km<sup>2</sup> under SSP5-8.5, whereas <i>B. alnoides</i> shows potential for southward range expansion despite some habitat loss. These findings underscore the urgent need for targeted conservation strategies to preserve Thai <i>Tuber</i> species and their symbiotic hosts under changing climatic conditions.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"835-846"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2025-09-01Epub Date: 2025-06-27DOI: 10.1080/00275514.2025.2496600
Paulo Henrique Campiteli, Maria Augusta Horta, Rafaela Rossi Rosolen, Juliano Sales Mendes, Carla Cristina da Silva, Danilo Sforça, Anete Pereira de Souza
{"title":"Targeting lignocellulolytic gene clusters in novel <i>Trichoderma atroviride</i> and <i>Trichoderma harzianum</i> strains through bacterial artificial chromosome-guided analysis.","authors":"Paulo Henrique Campiteli, Maria Augusta Horta, Rafaela Rossi Rosolen, Juliano Sales Mendes, Carla Cristina da Silva, Danilo Sforça, Anete Pereira de Souza","doi":"10.1080/00275514.2025.2496600","DOIUrl":"10.1080/00275514.2025.2496600","url":null,"abstract":"<p><p>Lignocellulosic biomass is a complex carbon source with recalcitrant properties whose degradation via industrial enzymatic hydrolysis is challenging, directly affecting the cost of reliable energy production. In nature, filamentous fungi, including <i>Trichoderma</i> species, degrade lignocellulose via an arsenal of hydrolytic and oxidative enzymes that act synergistically to process it into soluble sugar monomers. This work explored the genomic content of <i>Trichoderma atroviride</i> and <i>Trichoderma harzianum</i> strains with hydrolytic abilities by identifying regions possessing degradative enzyme-encoding genes, namely, hydrolytic clusters. We employed bacterial artificial chromosome (BAC) methodology to target specific genomic regions and explore their genetic organization, proximal gene context, and gene expression under degradative conditions. With this tool, it was possible to inspect the linear structure and expression profile of target hydrolytic-rich genomic regions. The present work offers a perspective on the organization of genome regions related to carbohydrate metabolism. This study revealed novel genes and genome regions that are positively regulated during cellulose degradation, contributing to elucidating differences in gene organization that potentially impact hydrolysis among <i>Trichoderma</i> species.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"884-898"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Leaf mycobiome across Fabaceae species: Non-nodulating plants exhibit higher fungal diversity.","authors":"Rachelle Fernandez-Vargas, Fabiana Tabash-Porras, Keilor Rojas-Jimenez","doi":"10.1080/00275514.2025.2527989","DOIUrl":"10.1080/00275514.2025.2527989","url":null,"abstract":"<p><p>The impact of plant-bacterial symbioses in roots on plant-fungal interactions in distant tissues, such as leaves, remains a significant knowledge gap, particularly for tropical legume trees. In this study, we analyzed the ITS2 sequences of fungal endophytes from eight tropical Fabaceae species, differing in their ability to form root nodules, to explore patterns in foliar fungal community composition. Our findings reveal differences in fungal community richness and composition between nodulating and non-nodulating species, with non-nodulating species hosting a richer and more diverse fungal community. Specifically, non-nodulating plants supported 72 taxonomic orders and 130.5 fungal amplicon sequence variants (ASVs), compared with 45 orders and 42.7 ASVs in nodulating plants. Moreover, 40% of fungal orders were exclusive to non-nodulating species. These patterns provide insights into the diversity of fungal endophytes in tropical legumes and lay the groundwork for future research on plant-microbe interactions. This study emphasizes the need for further exploration of the ecological factors influencing fungal community composition in tropical forest ecosystems.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"847-857"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144789585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2025-09-01Epub Date: 2025-07-29DOI: 10.1080/00275514.2025.2525718
Muhammad Salahudin Kheirel Anuar, Nur Hazwani Khairuddin, Zaishazleen Shazwanie Zainal, Muhammad Farhan Nazarudin, Mohammad Noor Amal Azmai, Annas Salleh, Che Azurahanim Che Abdullah, Ina Salwany Md Yasin, Mohd Termizi Yusof
{"title":"<i>Lecanicillium coprophilum-</i>mediated green synthesis of zinc oxide nanoparticles displaying antimicrobial activity against important rice pathogens.","authors":"Muhammad Salahudin Kheirel Anuar, Nur Hazwani Khairuddin, Zaishazleen Shazwanie Zainal, Muhammad Farhan Nazarudin, Mohammad Noor Amal Azmai, Annas Salleh, Che Azurahanim Che Abdullah, Ina Salwany Md Yasin, Mohd Termizi Yusof","doi":"10.1080/00275514.2025.2525718","DOIUrl":"10.1080/00275514.2025.2525718","url":null,"abstract":"<p><p>Spot and blight diseases are prevalent in the paddy sector, particularly in Malaysia, and are caused by the pathogens <i>Burkholderia glumae</i> and <i>Curvularia lunata</i>. To combat these issues, chemical pesticides, fungicides, and antibiotics are commonly used due to their affordability and ease of application. However, their excessive use has led to serious environmental issues, including groundwater pollution, soil contamination, and toxicity to non-target organisms. To address these concerns, nanotechnology, especially the \"green synthesis\" method, has recently gained attention. One approach uses metabolites from microbes as reducing agents to synthesize metal oxide nanoparticles, offering a more environmentally friendly alternative to conventional disease control methods. This study aims to explore the ability of a soil-derived fungal isolate to synthesize zinc oxide nanoparticles (ZnO-NPs) and to characterize the physicochemical properties and antimicrobial activity of the generated ZnO-NPs against paddy pathogens. In this study, a single fungal isolate capable of growing on potato dextrose agar (PDA) supplemented with 2 mM ZnSO<sub>4</sub>·7H<sub>2</sub>O was obtained from soil. The isolate, identified as <i>Lecanicillium coprophilum</i>, demonstrated the ability to synthesize ZnO-NPs. Characterization of the synthesized ZnO-NPs using various analytical techniques revealed distinct rod- and spherical shaped nanoparticles with a hexagonal wurtzite structure. Antimicrobial assessment showed that higher ZnO-NP concentrations led to greater inhibition of <i>B. glumae</i> and <i>C. lunata</i>. This study highlights the potential of green-synthesized ZnO-NPs as an eco-friendly alternative for managing paddy diseases.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"801-817"},"PeriodicalIF":2.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144743154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}