MycologiaPub Date : 2023-11-01Epub Date: 2023-11-14DOI: 10.1080/00275514.2023.2258268
Aaron S David, Damian J Hernandez, Eric S Menges, Vivienne L Sclater, Michelle E Afkhami, Christopher A Searcy
{"title":"Heterogeneous landscape promotes distinct microbial communities in an imperiled scrub ecosystem.","authors":"Aaron S David, Damian J Hernandez, Eric S Menges, Vivienne L Sclater, Michelle E Afkhami, Christopher A Searcy","doi":"10.1080/00275514.2023.2258268","DOIUrl":"10.1080/00275514.2023.2258268","url":null,"abstract":"<p><p>Habitat heterogeneity is a key driver of biodiversity of macroorganisms, yet how heterogeneity structures belowground microbial communities is not well understood. Importantly, belowground microbial communities may respond to any number of abiotic, biotic, and spatial drivers found in heterogeneous environments. Here, we examine potential drivers of prokaryotic and fungal communities in soils across the heterogenous landscape of the imperiled Florida scrub, a pyrogenic ecosystem where slight differences in elevation lead to large changes in water and nutrient availability and vegetation composition. We employ a comprehensive, large-scale sampling design to characterize the communities of prokaryotes and fungi associated with three habitat types and two soil depths (crust and subterranean) to evaluate (i) differences in microbial communities across these heterogeneous habitats, (ii) the relative roles of abiotic, biotic, and spatial drivers in shaping community structure, and (iii) the distribution of fungal guilds across these habitats. We sequenced soils from 40 complete replicates of habitat × soil depth combinations and sequenced the prokaryotic 16S and fungal internal transcribed spacer (ITS) regions using Illumina MiSeq. Habitat heterogeneity generated distinct communities of soil prokaryotes and fungi. Spatial distance played a role in structuring crust communities, whereas subterranean microbial communities were primarily structured by the shrub community, whose roots they presumably interacted with. This result helps to explain the unexpected transition we observed between arbuscular mycorrhiza-dominated soils at low-elevation habitats to ectomycorrhiza-dominated soils at high-elevation habitats. Our results challenge previous notions of environmental determinism of microbial communities and generate new hypotheses regarding symbiotic relationships across heterogeneous environments.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"739-748"},"PeriodicalIF":2.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41182998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2023-11-01Epub Date: 2023-11-14DOI: 10.1080/00275514.2023.2259277
Tatiana Yu Gagkaeva, Aleksandra S Orina, Maria M Gomzhina, Olga P Gavrilova
{"title":"<i>Fusarium bilaiae</i>, a new cryptic species in the <i>Fusarium fujikuroi</i> complex associated with sunflower.","authors":"Tatiana Yu Gagkaeva, Aleksandra S Orina, Maria M Gomzhina, Olga P Gavrilova","doi":"10.1080/00275514.2023.2259277","DOIUrl":"10.1080/00275514.2023.2259277","url":null,"abstract":"<p><p>A <i>Fusarium</i> species associated with sunflower based on multilocus genealogy, morphological, physiological, ecological, mating type, and mycotoxin production data is formally described as the newly discovered species <i>Fusarium bilaiae</i>. The <i>F. bilaiae</i> strains formed a genealogically exclusive lineage within the African clade of the <i>F. fujikuroi</i> species complex. Comparison of morphological characteristics of <i>F. bilaiae</i> strains with those of the closely related <i>F. phyllophilum</i> strain NRRL 13617 revealed similarities in the main micromorphology of both species: production of numerous one-celled microconidia in false heads and short chains on monophialides and polyphialides and the absence of macroconidia and sporodochia. There was a slight but significant distinction between the two species when the strains were grown on different agar media, as well as in the shape and width of microconidia. <i>Fusarium bilaiae</i> strains isolated from symptomatic sunflower were not pathogenic to members of the Asteraceae tested; apparently, they live as saprophytes or endophytes in sunflower tissues. A difference between the strains of the two species in the production of mycotoxins was demonstrated with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) analysis. On autoclaved rice, <i>F. bilaiae</i> did not produce fumonisins and beauvericin but produced moniliformin, whereas <i>F. phyllophilum</i> produced all these mycotoxins. A polymerase chain reaction (PCR) assay specific for mating type alleles identified <i>F. bilaiae</i> as a putative heterothallic species with <i>MAT1-1</i> and <i>MAT1-2</i> idiomorphs, but laboratory crosses were unsuccessful. Determining the area and host range of the new endophytic species <i>F. bilaiae</i> is a priority for future research.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"787-801"},"PeriodicalIF":2.8,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71413135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2023-09-01Epub Date: 2023-07-26DOI: 10.1080/00275514.2023.2228182
Cassandra L Ettinger, Talieh Ostovar, Mark Yacoub, Steven Ahrendt, Robert H Hice, Brian A Federici, Jason E Stajich
{"title":"Genomes and transcriptomes help unravel the complex life cycle of the blastoclad fungus, <i>Coelomomyces lativittatus</i>, an obligate parasite of mosquitoes and microcrustaceans.","authors":"Cassandra L Ettinger, Talieh Ostovar, Mark Yacoub, Steven Ahrendt, Robert H Hice, Brian A Federici, Jason E Stajich","doi":"10.1080/00275514.2023.2228182","DOIUrl":"10.1080/00275514.2023.2228182","url":null,"abstract":"<p><p>Species of the phylum Blastocladiomycota, early-diverging zoosporic (flagellated) lineages of fungi, are vastly understudied. This phylum includes the genus <i>Coelomomyces</i>, which consists of more than 80 fungal species that are obligate parasites of arthropods. Known <i>Coelomomyces</i> species lack a complete asexual life cycle, instead surviving through an obligate heterecious alternation of generations life cycle. Despite their global distribution and interesting life cycle, little is known about the genomics of any <i>Coelomomyces</i> species. To address this, we generated three draft-level genomes and annotations for <i>C. lativittatus</i> representing its haploid meiospore, orange gamete, and amber gamete life stages. These draft genome assemblies ranged in size from 5002 to 5799 contigs, with a total length of 19.8-22.8 Mb and a mean of 7416 protein-coding genes. We then demonstrated the utility of these genomes by combining the draft annotations as a reference for analysis of <i>C. lativittatus</i> transcriptomes. We analyzed transcriptomes from across host-associated life stages, including infected larvae and excised mature sporangia from the mosquito <i>Anopheles quadrimaculatus</i>. We identified differentially expressed genes and enriched GO terms both across and within life stages and used these to make hypotheses about <i>C. lativittatus</i> biology. Generally, we found the <i>C. lativittatus</i> transcriptome to be a complex and dynamic expression landscape; GO terms related to metabolism and transport processes were enriched during infection and terms related to dispersal were enriched during sporulation. We further identified five high mobility group (HMG)-box genes in <i>C. lativittatus</i>, three belonging to clades with mating type (MAT) loci from other fungi, as well as four ortholog expansions in <i>C. lativittatus</i> compared with other fungi. The <i>C. lativittatus</i> genomes and transcriptomes reported here are a valuable resource and may be leveraged toward furthering understanding of the biology of these and other early-diverging fungal lineages.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":"115 5","pages":"630-647"},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10575185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2023-09-01DOI: 10.32598/bcn.2021.2892.1
Emel Uzunoglu-Ozyurek, Gizem Önal, Serap Dökmeci
{"title":"Investigating the Therapeutics Effects of Oral Cavity Derived Stem Cells on Neurodegenerative Diseases: A Systematic Review.","authors":"Emel Uzunoglu-Ozyurek, Gizem Önal, Serap Dökmeci","doi":"10.32598/bcn.2021.2892.1","DOIUrl":"10.32598/bcn.2021.2892.1","url":null,"abstract":"<p><strong>Introduction: </strong>Published data obtained from in vitro and in vivo studies was reviewed systematically and analyzed critically to evaluate the effect of oral cavity-derived stem cells (OCDSCs) on the recovery or therapy of neurodegenerative diseases (NDs), such as Alzheimer disease (AD), amyotrophic lateral sclerosis (ALS), Huntington (HD) diseases, and Parkinson disease (PD).</p><p><strong>Methods: </strong>An electronic search was accomplished. References of included articles were also manually searched. Studies were critically evaluated for suitability against the inclusion/exclusion criteria and the data was extracted. Bias risk evaluation of the studies and evidence synthesis were conducted.</p><p><strong>Results: </strong>A total of 14 in vivo and 10 in vitro studies met the inclusion criteria. PD was induced in 10 in vivo and 7 in vitro studies, while AD was induced in 2 in vivo and 4 in vitro studies. Two studies (1 in vitro and 1 in vivo) evaluated ALS disease and 1 in vivo study evaluated HD. Moderate evidence was found for in vitro studies reporting the positive effect of OCDSCs on PD or AD recovery. Strong evidence was found for in vivo studies in which PD animal models were used; meanwhile, moderate evidence was found for the impact of OCDSCs on AD recovery. Limited evidence was found for in vivo studies evaluating HD and ALS.</p><p><strong>Conclusion: </strong>Although studies reported favorable data regarding the OCDSCs on NDs, they presented a considerable risk of bias. Because of heterogeneous study characteristics, the current study recommends improving standardized methods to evaluate the therapeutic effects of OCDSCs on the NDs.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":"79 1","pages":"565-584"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11016878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80906053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2023-09-01Epub Date: 2023-07-26DOI: 10.1080/00275514.2023.2227747
Ingrid Gomes Duarte, Ana Gabriele Gurgel Amaral, Willie Anderson Dos Santos Vieira, Josiene Silva Veloso, Anthony Carlos da Silva, Christiana de Fátima Bruce da Silva, Valdir de Queiroz Balbino, Lisa A Castlebury, Marcos Paz Saraiva Câmara
{"title":"Diversity of <i>Colletotrichum</i> species associated with torch ginger anthracnose.","authors":"Ingrid Gomes Duarte, Ana Gabriele Gurgel Amaral, Willie Anderson Dos Santos Vieira, Josiene Silva Veloso, Anthony Carlos da Silva, Christiana de Fátima Bruce da Silva, Valdir de Queiroz Balbino, Lisa A Castlebury, Marcos Paz Saraiva Câmara","doi":"10.1080/00275514.2023.2227747","DOIUrl":"10.1080/00275514.2023.2227747","url":null,"abstract":"<p><p>Anthracnose caused by <i>Colletotrichum</i> species is one of the most important diseases of torch ginger. The disease leads to loss of aesthetic and commercial value of torch ginger stems. This study aimed to characterize <i>Colletotrichum</i> species associated with torch ginger anthracnose in the production areas of Pernambuco and Ceará. A total of 48 <i>Colletotrichum</i> isolates were identified using molecular techniques. Pathogenicity tests were performed on torch ginger with representative isolates. Phylogenetic analyses based on seven loci-DNA lyase (<i>APN</i>2), intergenic spacer between DNA lyase and the mating-type locus <i>MAT1-2-1</i> (<i>APN</i>2/<i>MAT</i>-IGS), calmodulin (<i>CAL</i>), intergenic spacer between glyceraldehyde-3-phosphate dehydrogenase (<i>GAPDH</i>) and a hypothetical protein (<i>GAP</i>2-IGS), glutamine synthetase (<i>GS</i>), and β-tubulin (<i>TUB</i>2)-revealed that they belong to five known <i>Colletotrichum</i> species, namely, <i>C. chrysophilum, C. fructicola, C. siamense, C. theobromicola</i>, and <i>C. tropicale</i>, and three newly discovered species, described here as <i>C. atlanticum, C. floscerae</i>, and <i>C. zingibericola</i>. Of these, <i>C. atlanticum</i> was the most dominant. Pathogenicity assays showed that all isolates were pathogenic to torch ginger bracts. All species are reported for the first time associated with torch ginger in Brazil. The present study contributes to the current understanding of the diversity of <i>Colletotrichum</i> species associated with anthracnose on torch ginger and demonstrates the importance of accurate species identification for effective disease management strategies.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":"115 5","pages":"661-673"},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10575184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2023-09-01Epub Date: 2023-07-06DOI: 10.1080/00275514.2023.2220166
Achala J Gajanayake, Samantha C Karunarathna, Ruvishika S Jayawardena, Thatsanee Luangharn, Abhaya Balasuriya
{"title":"Fungicolous <i>Mucor</i> on mushrooms: One novel species and six host records from southwest China and northern Thailand.","authors":"Achala J Gajanayake, Samantha C Karunarathna, Ruvishika S Jayawardena, Thatsanee Luangharn, Abhaya Balasuriya","doi":"10.1080/00275514.2023.2220166","DOIUrl":"10.1080/00275514.2023.2220166","url":null,"abstract":"<p><p><i>Mucor</i> species are a group of common soil-borne fungi, known to cause infections on humans and animals, interfere in food production, and act as useful agents in biotechnological applications. This study reports one new <i>Mucor</i> species, <i>M. yunnanensis</i>, which was found to be fungicolous on an <i>Armillaria</i> sp. from southwest China. Further, <i>M. circinelloides</i> on <i>Phlebopus</i> sp., <i>M. hiemalis</i> on <i>Ramaria</i> sp. and <i>Boletus</i> sp., <i>M. irregularis</i> on <i>Pleurotus</i> sp., <i>M. nederlandicus</i> on <i>Russula</i> sp., and <i>M. yunnanensis</i> on <i>Boletus</i> sp. are reported as new host records. <i>Mucor yunnanensis</i> and <i>M. hiemalis</i> have been collected from Yunnan Province in China, whereas <i>M. circinelloides, M. irregularis</i>, and <i>M. nederlandicus</i> have been collected from Chiang Mai and Chiang Rai Provinces in Thailand. All the <i>Mucor</i> taxa reported herein were identified based on both morphology and phylogenetic analyses of a combined nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS) and partial nuc 28S rDNA (28S) sequence matrix. Comprehensive descriptions, illustrations, and a phylogenetic tree are provided for all the taxa reported in the study to show the placements of taxa, and the new taxon is compared with its sister taxa.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":"115 5","pages":"674-692"},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10558818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2023-09-01Epub Date: 2023-08-10DOI: 10.1080/00275514.2023.2234269
Jing Luo, Emily Walsh, Glen Groben, Brandon Justiniano, Ning Zhang
{"title":"Larger presence of ectomycorrhizae detected from pygmy pine ecotype in the fire-frequent pine barrens ecosystem.","authors":"Jing Luo, Emily Walsh, Glen Groben, Brandon Justiniano, Ning Zhang","doi":"10.1080/00275514.2023.2234269","DOIUrl":"10.1080/00275514.2023.2234269","url":null,"abstract":"<p><p>Pine barrens ecosystem has acidic, sandy, and nutrient-poor soil and is prone to drought and fire. In the New Jersey Pine Barrens, the predominant pitch pine (<i>Pinus rigida</i>) consists of two ecotypes: the regular pitch pines with heights of 4.6-12 m, and the pygmy pines of low stature (1.2-1.8 m) in the New Jersey Pine Plains. Previous ecological studies suggested that the dwarf pines in the Pine Plains that are embedded within the Pine Barrens were an evolutionary adaptation to frequent fire. Pines are obligate ectomycorrhizal (EcM) mutualists, and their root mycobiota may contribute to stress protection and plant health. However, information on the mycobiota associated with plants in the pine barrens ecosystem is lacking. To have a holistic understanding of the evolution and adaptation in this stressed environment, we used both culture-independent metabarcoding and culture-based method to characterize the mycobiota from soil and root of the two ecotypes and to identify core mycobiota. We found that Agaricomycetes, Leotiomycetes, and Mucoromycotina are predominant fungi in the New Jersey Pine Barrens ecosystem, which is rich in root mutualistic fungi. We observed that the pygmy pine roots had significantly higher density of EcM tips than the regular pine roots. This was corroborated by our metabarcoding analysis, which showed that the pygmy pine trees had higher ratio of ectomycorrhiza-forming fungi than the regular-statured pines. We hypothesize that symbiotrophic EcM fungi associated with pygmy pines are capable of mitigating high fire stress in the Pine Plains.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":"115 5","pages":"602-613"},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10199699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2023-09-01Epub Date: 2023-07-21DOI: 10.1080/00275514.2023.2227553
Bjarke Jensen
{"title":"Patterns of basidiocarp growth in oak mazegill (<i>Daedalea quercina</i>, Polyporales) revealed by experimental and natural perturbations.","authors":"Bjarke Jensen","doi":"10.1080/00275514.2023.2227553","DOIUrl":"10.1080/00275514.2023.2227553","url":null,"abstract":"<p><p>In Polyporales, the pore field immediately behind the basidiocarp margin may configure the hymenophore. Basidiocarp growth is not restricted to the margin, however. Here, the importance of the pore field was assessed from two years' of observations on naturally occurring oak mazegill (<i>Daedalea quercina</i>, Polyporales) basidiocarps and tested by experimental perturbations in natural habitats. Oak mazegill was chosen because the formed hymenophore has a unique and stable combination of poroid and lamellate features. Whether the pore field is required for basidiocarp growth was tested in 10 basidiocarps in which one side was resected. New growth was observed in six basidiocarps, and it occurred equally from the cut hymenophore and the intact pore field. New formation of hymenophore and pileus even occurred in seven out of 10 basidiocarps that had the entire pore field resected. Whether the hymenophore is configured permanently was tested on 54 basidiocarps on 10 trunks that were turned upside down. A new hymenophore grew through the old pileus, often far from the pore field, and its hymenophore configuration was invariably poroid despite the old hymenophore had lamellate features. In 48 experimentally banded basidiocarps, new hymenophore grew in the insertion hole of the band despite this being far from the pore field. The banded basidiocarps grew at an average rate of 5 mm per year. In conclusion, the capacity to configure the hymenophore is not confined to the pore field and it could be broadly present in the basidiocarp, possibly due to ubiquitous hyphal totipotency.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":"115 5","pages":"648-660"},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10208717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2023-09-01Epub Date: 2023-07-18DOI: 10.1080/00275514.2023.2224699
Desiree Szarka, Nicole A Gauthier, Mostafa Rahnama, Christopher L Schardl
{"title":"Seeing double on <i>Cannabis</i>: Haploids and heteroploids of <i>Bipolaris gigantea</i> on hemp and other dicots.","authors":"Desiree Szarka, Nicole A Gauthier, Mostafa Rahnama, Christopher L Schardl","doi":"10.1080/00275514.2023.2224699","DOIUrl":"10.1080/00275514.2023.2224699","url":null,"abstract":"<p><p><i>Bipolaris gigantea</i> (= <i>Drechslera gigantea</i>) causes Bipolaris leaf spot (BLS), a devastating and widespread disease on industrial hemp (<i>Cannabis sativa</i>). An investigation of relationships of isolates from hemp and other plants indicated variation in ploidy that has not previously been reported for <i>Bipolaris</i>. Isolates were obtained from BLS lesions on hemp and nearby weeds in 11 Kentucky counties and were similar to each other in morphology and growth characteristics. In total, 23 isolates were analyzed by multilocus phylogenetics, of which seven were also chosen for whole genome shotgun sequencing. Genes for RNA polymerase II subunit 2 (<i>RPB2</i>), translation elongation factor 1-α (<i>TEF1</i>), and mating type (<i>MAT1</i>) indicated that 13 of the isolates were haploid with only a single allele each of <i>RPB2</i> and <i>TEF1</i> and either the <i>MAT1-1</i> or <i>MAT1-2</i> idiomorph, whereas 10 were apparently \"heteroploid\" with two alleles each of <i>RPB2</i> and <i>TEF1</i> and both <i>MAT1</i> idiomorphs. Haploids all had identical <i>RPB2</i> alleles except for a 1-bp difference in two isolates, identical <i>TEF1</i> alleles, and (if present) identical <i>MAT1-2</i> alleles. Those alleles were also present in each heteroploid along with either of two related but distinct alleles for each gene. In contrast, haploids and heteroploids shared allelic variation of <i>MAT1-1</i>. In total, four haploid and two heteroploid genotypes were identified. Genome sequence data assembled to 30-32 Mb for each of four haploid isolates, but 10-31 Mb larger sizes for each of three heteroploids depending on sequencing platform and assembly program. The haploids and heteroploids caused similar disease on hemp.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":"115 5","pages":"614-629"},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10207345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycologiaPub Date : 2023-09-01Epub Date: 2023-07-21DOI: 10.1080/00275514.2023.2226893
Zheng-Mi He, Zhu L Yang
{"title":"A contribution to the knowledge of the genus <i>Infundibulicybe</i> (Tricholomatineae, Agaricales) in China: Two new species and five redescribed taxa.","authors":"Zheng-Mi He, Zhu L Yang","doi":"10.1080/00275514.2023.2226893","DOIUrl":"10.1080/00275514.2023.2226893","url":null,"abstract":"<p><p>In China, species of the genus <i>Infundibulicybe</i> are often confused with other taxa and misdetermined. Here we describe two newly discovered species, <i>I. bispora</i> and <i>I. ellipsospora</i>, and redescribe five known taxa of this genus present in China. These identifications are supported by both morphological and DNA-based phylogenetic evidence. Additionally, a key to all known species of <i>Infundibulicybe</i> is provided.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":"115 5","pages":"693-713"},"PeriodicalIF":2.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10208720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}