{"title":"Multilocus phylogeny contributes to fungal taxonomy with three novel species of <i>Conocybe</i> (Bolbitiaceae) from southern Punjab, Pakistan.","authors":"Muhammad Asif, Malka Saba, Fazli Maula, Wajahat Akram, Asif Kamal, Mubashar Raza","doi":"10.1080/00275514.2025.2463279","DOIUrl":null,"url":null,"abstract":"<p><p>Advancements in fungal taxonomy have been significantly enhanced by multilocus phylogenetic analyses, which improve the precision of species identification. This study also employs such methods to investigate the genus <i>Conocybe</i>, resulting in the description of three novel species, viz. <i>C. hasiluprensis</i> and <i>C. sultanii</i> belonging to the section <i>Pilosellae</i> and <i>C. ferruginae</i> of section <i>Conocybe</i>, from southern Punjab, Pakistan. Field collections yielded specimens that were examined morphologically and genetically, using a multilocus phylogenetic analysis utilizing sequences from the ITS, 28S, and <i>TEF1-α</i> regions. A combined phylogenetic tree was constructed through maximum likelihood and Bayesian inference methods, revealing three novel taxa occupying their respective distinct branches. Further, morphological analysis complemented the genetic data, with each species displaying unique characteristics. This study not only adds to the fungal biodiversity of Pakistan but also provides a robust framework for future taxonomic and ecological research in the region. In addition, we provide a key to the known species of <i>Conocybe</i> in Pakistan.</p>","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"1-17"},"PeriodicalIF":2.6000,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycologia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/00275514.2025.2463279","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Advancements in fungal taxonomy have been significantly enhanced by multilocus phylogenetic analyses, which improve the precision of species identification. This study also employs such methods to investigate the genus Conocybe, resulting in the description of three novel species, viz. C. hasiluprensis and C. sultanii belonging to the section Pilosellae and C. ferruginae of section Conocybe, from southern Punjab, Pakistan. Field collections yielded specimens that were examined morphologically and genetically, using a multilocus phylogenetic analysis utilizing sequences from the ITS, 28S, and TEF1-α regions. A combined phylogenetic tree was constructed through maximum likelihood and Bayesian inference methods, revealing three novel taxa occupying their respective distinct branches. Further, morphological analysis complemented the genetic data, with each species displaying unique characteristics. This study not only adds to the fungal biodiversity of Pakistan but also provides a robust framework for future taxonomic and ecological research in the region. In addition, we provide a key to the known species of Conocybe in Pakistan.
期刊介绍:
International in coverage, Mycologia presents recent advances in mycology, emphasizing all aspects of the biology of Fungi and fungus-like organisms, including Lichens, Oomycetes and Slime Molds. The Journal emphasizes subjects including applied biology, biochemistry, cell biology, development, ecology, evolution, genetics, genomics, molecular biology, morphology, new techniques, animal or plant pathology, phylogenetics, physiology, aspects of secondary metabolism, systematics, and ultrastructure. In addition to research articles, reviews and short notes, Mycologia also includes invited papers based on presentations from the Annual Conference of the Mycological Society of America, such as Karling Lectures or Presidential Addresses.