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[Production of Antimicrobial Polypeptides by Propionibacterium freudenreichii RVS-4-irf]. [弗氏丙酸杆菌RVS-4-irf抗菌多肽的生产]。
Mikrobiologiia Pub Date : 2017-03-01
E P Ryzhkovaa, I L Shamraichuk, A V Kurakov, A I Netrusov
{"title":"[Production of Antimicrobial Polypeptides by Propionibacterium freudenreichii RVS-4-irf].","authors":"E P Ryzhkovaa,&nbsp;I L Shamraichuk,&nbsp;A V Kurakov,&nbsp;A I Netrusov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Propionibacterium freudenreichii RVS-4-irf is a probiotic bacterium producing antimicrobial\u0000exometabolites applicable for foodstuff protection. Production of antimicrobial factors other than lowmolecular\u0000propionates was observed in media with and without trypton. A method was developed for production\u0000of the fraction of high-molecular mass exopolymers from the culture liquid. Their polypeptide nature was\u0000confirmed using proteinase K. The preparation of extracellular polypeptides from P. freudenreichii RVS-4-irf\u0000exhibited species-specific activity in suppression of fungal and bacterial growth.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36609286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Secondary Metabolites of Fungi of the Usti Section, Genus Aspergillus and Their Application in Chemosystematics]. 曲霉属真菌的次生代谢物及其在化学系统学中的应用
Mikrobiologiia Pub Date : 2017-03-01
A G Kozlovskii, T V Antipova, V P Zhelifonova, B P Baskunov, N E Ivanushkina, G A Kochkina, S M Ozerskaya
{"title":"[Secondary Metabolites of Fungi of the Usti Section, Genus Aspergillus and Their Application in Chemosystematics].","authors":"A G Kozlovskii,&nbsp;T V Antipova,&nbsp;V P Zhelifonova,&nbsp;B P Baskunov,&nbsp;N E Ivanushkina,&nbsp;G A Kochkina,&nbsp;S M Ozerskaya","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Secondary metabolites of 22 fungal strains (genus Aspergillus, section Usti) isolated at diverse geographic\u0000regions, including the Arctic permafrost deposits, were studied. The studied strains were found to\u0000synthesize a variety of biologically active compounds, structurally identified as drimane sesqueterpenoids,\u0000isoquinoline alkaloids (TMC-120 A−C, derivative 1), meroterpenoids (austalides О and J), and anthraquinone\u0000pigments (averufin, versicolorin C). Desferritriacetylfusigen production by A. calidoustus isolates is\u0000reported for the first time. The individual spectra of secondary metabolites were used for reidentification of\u000017 strains, of which 15 were identified as A. calidoustus and two, as A. pseudodeflectus.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36609288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Morphological, Physiological, and Biochemical Characteristics of a Benzoate-Degrading Strain Rhodococcus opacus 1CP under Stress Conditions]. [胁迫条件下苯甲酸酯降解菌株不透明红球菌1CP的形态、生理和生化特性]。
Mikrobiologiia Pub Date : 2017-03-01
I P Solyanikova, N E Suzina, E V Emelyanova, V N Polivtseva, A B Pshenichnikova, A G Lobanok, L A Golovleva
{"title":"[Morphological, Physiological, and Biochemical Characteristics of a Benzoate-Degrading Strain Rhodococcus opacus 1CP under Stress Conditions].","authors":"I P Solyanikova,&nbsp;N E Suzina,&nbsp;E V Emelyanova,&nbsp;V N Polivtseva,&nbsp;A B Pshenichnikova,&nbsp;A G Lobanok,&nbsp;L A Golovleva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Ability of actinobacteria Rhodococcus opacus 1CP to survive under unfavorable conditions and retain its biodegradation activity was assessed. The morphological and ultrastructural features of R. opacus 1CP cells degrading benzoate in the presence of oxidants and stress-protecting agents were investigated. The cells of R. opacus 1CP were resistant to oxidative stress caused by up to 100 mM H2O2 or up to 25 μM juglone (5-oxy-1,4-naphthoquinone). After 2 h of stress impact, changes in the fatty acid composition, increased activity of antioxidant enzymes, and changes in cell morphology and ultrastructure were observed. The strain retained its ability to degrade benzoate. Quercetin had a protective effect on benzoate-degrading cells of R. opacus 1CP. The strategy for cells survival under unfavorable conditions was formulated, which included\u0000decreased cell size/volume and formation of densely-packed cell conglomerates, in which the cells are embedded into a common matrix. Formation of conglomerates may probably be considered as a means for protecting the cells against aggressive environmental factors. The multicellular conglomerate structure and the matrix material impede the penetration of toxic substances into the conglomerates, promoting survival of the cells located inside.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36568658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Taxonomic Composition of Lake Baikal Bacterioneuston Communities]. [贝加尔湖细菌菌群的分类组成]。
Mikrobiologiia Pub Date : 2017-03-01
A D Galach’yants, N L Bel’kova, E V Sukhanova, Yu P Galach’yants, A A Morozov, V V Parfenova
{"title":"[Taxonomic Composition of Lake Baikal Bacterioneuston Communities].","authors":"A D Galach’yants,&nbsp;N L Bel’kova,&nbsp;E V Sukhanova,&nbsp;Yu P Galach’yants,&nbsp;A A Morozov,&nbsp;V V Parfenova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The taxonomic composition of microbial communities of Lake Baikal surface microlayer was studied by pyrosequencing of the 16S rDNA amplicons. Statistically reliable differences were found between bacterioneuston of the shallow and deep-water stations. The shallow station community was characterized by higher diversity than the deep-water one. While bacterioneuston communities were shown to be less diverse\u0000than the water column communities, their diversity was comparable to that of other biofilm associations. Microbial communities of Lake Baikal surface microlayer were shown to be similar to those of the water column in the composition of predominant phyla, while differing considerably at the genus level. Bacterioneuston of Lake Baikal was comparable to microbial communities of the surface microlayer of other freshwater basins, although it was characterized by high abundance of the Alphaproteobacteria and Verrucomicrobia. High abundance of photoheterotrophs compared to the water column communities of other freshwater basins was another distinctive feature of Lake Baikal bacterioneuston. Our results showed the Lake Baikal surface microlayer to be a specific microbial community with low species diversity and relatively high abundance of photoheterotrophic microorganisms.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36569620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of chitinase diversity in the Baltic Sea bottom sediments. 波罗的海海底沉积物几丁质酶多样性分析。
Mikrobiologiia Pub Date : 2017-01-01
A V Teplyuk, N I Samarov, A A Korzhenkov, M O Ul’yanova, M A Goeva, I V Kublanov, T A Kanapatskii, N V Pimenov, S V Toshchakov
{"title":"Analysis of chitinase diversity in the Baltic Sea bottom sediments.","authors":"A V Teplyuk,&nbsp;N I Samarov,&nbsp;A A Korzhenkov,&nbsp;M O Ul’yanova,&nbsp;M A Goeva,&nbsp;I V Kublanov,&nbsp;T A Kanapatskii,&nbsp;N V Pimenov,&nbsp;S V Toshchakov","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36483957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic Polymorphism of Sherry Saccharomyces cerevisiae Yeasts. 雪利酒酵母菌的遗传多态性。
Mikrobiologiia Pub Date : 2017-01-01
G I Naumov
{"title":"Genetic Polymorphism of Sherry Saccharomyces cerevisiae Yeasts.","authors":"G I Naumov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The review deals with natural diversity of sherry (“flor”) Saccharomyces cerevisiae yeasts. Various\u0000properties of these yeasts are analyzed: life cycles, fermentation of sugars, sensitivity to methyl violet and killer\u0000toxins, and resistance to ethanol and sulfite. Special attention is paid to molecular identification and differentiation\u0000of sherry yeasts. The history of their nomenclature is considered, including the names of possible\u0000subpopulations: “aceti,” “beticus” (“cheresienses”), “cordubensis,” “gaditensis,” “hispanica” (“prostoserdovii”),\u0000and “oxidans.”</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36484723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of chromate ions on marine microalgae Phaeodactylum tricornutum. 铬酸盐离子对海洋微藻褐指藻的影响。
Mikrobiologiia Pub Date : 2017-01-01
D T Gabbasova, D N Matorin, I V Konyukhov, N Kh Seifullina, B K Zayadan
{"title":"Effect of chromate ions on marine microalgae Phaeodactylum tricornutum.","authors":"D T Gabbasova,&nbsp;D N Matorin,&nbsp;I V Konyukhov,&nbsp;N Kh Seifullina,&nbsp;B K Zayadan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Effect of chromate ions on the culture of a marine diatom Phaeodactylum tricornutum was studied using an M-PEA-2 fluorimeter, which carries out simultaneous measurement of fluorescence induction and redox transformations of the P700 pigment within a millisecond range. Chromate ions were shown to inhibit electron transport in PS II and decrease the rate of QА reduction. This results in decreased values of the quantum yield of electron transport in PS II (ϕEo) and performance index (PI ABS), lower rates of P700 reduction, and increased energy (DI0/RC) and ΔpH-dependent nonphotochemical quenching (q E ). Emergence of the slow component of P700 reduction was observed, indicating the activation of cyclic transport in the presence of chromate. Performance index (PI ABS), which was the most sensitive parameter, may be recommended for detection of chromate ions at early stages of their toxic action. The fluorescence parameter F O is promising application in biotesting to assess the algal growth rates.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36484729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diversity of magnetotactic bacteria of the Moskva River. 莫斯科河趋磁细菌的多样性。
Mikrobiologiia Pub Date : 2017-01-01
V V Kozyaeva, D S Grouzdev, M V Dziuba, T V Kolganova, B B Kuznetsov
{"title":"Diversity of magnetotactic bacteria of the Moskva River.","authors":"V V Kozyaeva,&nbsp;D S Grouzdev,&nbsp;M V Dziuba,&nbsp;T V Kolganova,&nbsp;B B Kuznetsov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Diversity of magnetotactic bacteria in the Moskva River at the Strogino area was studied using microscopy and phylogenetic analysis. Magnetotactic cocci were the predominant morphotype. Phylogenetic analysis of the 16S rRNA gene sequences revealed 13 OTUs of the orders Magnetococcales and Rhodospirillales, class Alphaproteobacteria. The shares of the relevant sequences were 90 and 10%, respectively. An axenic culture of magnetotactic spirilla was isolated from the studied community. According to the results of the 16S rRNA gene sequencing, the isolate was identified as a new Magnetospirillum species.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36485139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biodiversity of aerobic methylobacteria associated with the phyllosphere of the southern Moscow Oblast. 与莫斯科州南部层状圈相关的需氧甲基细菌的生物多样性。
Mikrobiologiia Pub Date : 2017-01-01
E N Mikrobiologiia, N V Doronina, I I Mustakhimov, N V Agafonova, Yu A Trotsenko
{"title":"Biodiversity of aerobic methylobacteria associated with the phyllosphere of the southern Moscow Oblast.","authors":"E N Mikrobiologiia,&nbsp;N V Doronina,&nbsp;I I Mustakhimov,&nbsp;N V Agafonova,&nbsp;Yu A Trotsenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>During the summer period (15–25°C), 34 strains of methylotrophic bacteria associated with different\u0000species of herbs, shrub, and trees in Pushchino (Moscow oblast, Russia) were isolated on the medium\u0000with methanol. Predominance of pink-colored Methylobacterium strains in the phyllosphere of many plants\u0000was confirmed by microscopy, enumeration of the colonies from grass leaves, and sequencing of the 16S\u0000rRNA genes. Colorless and yellow-pigmented methylotrophs belonged to the genera Methylophilus, Methylobacillus,\u0000Hansschlegelia, Methylopila, Xanthobacter, and Paracoccus. All isolates were able to synthesize plant\u0000hormones auxins from L-tryptophan (5−50 μg/mL) and are probably plant symbionts.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36484600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Formation of Pectobacterium carotovorum biofilms depending of the carbon source. 胡萝卜乳杆菌生物膜的形成取决于碳源。
Mikrobiologiia Pub Date : 2017-01-01
A L Turskaya, A A Ul’danova, A V Stepanov, Yu S Bukin, V V Verkhoturov, B K Gaida, Yu A Markova
{"title":"Formation of Pectobacterium carotovorum biofilms depending of the carbon source.","authors":"A L Turskaya,&nbsp;A A Ul’danova,&nbsp;A V Stepanov,&nbsp;Yu S Bukin,&nbsp;V V Verkhoturov,&nbsp;B K Gaida,&nbsp;Yu A Markova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Microbial biofilms are a basic form of existence of bacteria in the environment, as well as in the animal and plant organisms. The patterns of biofilm formation depending on cultivation conditions is presently insufficiently studied. This paper presents experimental results on the effect of carbon sources on biofilm formation and movement on the swarming type in a phytopathogenic bacterium Pectobacterium carotovorum. A polyol inositol was shown to cause a significant activation of these processes.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36484726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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