Mikrobiologiia最新文献_第3页

Genome analysis of Acidiplasma sp. MBA-1, a polyextremophilic archaeon predominant in the microbial community of a bioleaching reactor. 在生物浸出反应器微生物群落中占优势的嗜极古菌Acidiplasma sp. MBA-1的基因组分析。

Mikrobiologiia Pub Date : 2017-01-01

A G Bulaev, A V Kanygina, A I Manolov

{"title":"Genome analysis of Acidiplasma sp. MBA-1, a polyextremophilic archaeon predominant in the microbial community of a bioleaching reactor.","authors":"A G Bulaev, A V Kanygina, A I Manolov","doi":"","DOIUrl":"","url":null,"abstract":"Results of genome analysis of a member of the family Ferroplasmaceae, Acidiplasma sp. strain MBA-1, an extremely acidophilic, moderately thermophilic archaeon oxidizing ferrous iron under oxic conditions and utilizing organic compounds. This strain was previously shown to predominate in the community carrying out biooxidation of pyrite-arsenopyrite gold-bearing concentrate. The genome was sequenced using the Illumina HiSeq 2000 platform. A total of 2306800 pairwise reads were obtained, corresponding to 300-fold coverage. Assembly was carried out by three programs in parallel. The optimal assembly contained nine contigs, the genome size was 1747364 bp, and N50 was 446845 bp. Annotation of the genome revealed 1749 protein-encoding sequences, as well as 46 tRNA genes and one rRNA gene copy. The results of genome analysis confirmed the previous data on the physiology of this organism. The gene of sulfocyanin (TZ01_06185), a blue copper-containing protein playing the key role in the iron-oxidizing electron transport chain, was identified in the genome. The genes encoding sulfur oxidoreductase (TZ01_04750) and sulfateadenilyl transferase (TZ01_04545), the enzymes of sulfur oxidation, were also identified. The genes involved in the transport and catabolism of organic compounds and the genes of the 3-hydroxypropionate/4-hydroxybutyrate cycle were revealed. The genome of Acidiplasma sp. MBA-1 is the first genome of this genus deposited to a public database DDBJ/EMBL/GenBank (accession no. JYHS00000000) and is of interest for further investigation of Acidiplasma archaea.","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36485137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of polyethylenimine-based sorbents on the luminescence of photobacteria. 聚乙烯亚胺基吸附剂对光细菌发光性能的影响。

Mikrobiologiia Pub Date : 2017-01-01

E S Lobakova, L E Aleskerova, A A Orlova, S G Vasil’eva, A D Ismailov

引用次数: 0

Sporulation of Bacillus subtilis in Binary Cultures with Ultramicrobacteria. 超微细菌二元培养枯草芽孢杆菌的产孢。

Mikrobiologiia Pub Date : 2017-01-01

A P Shorokhova, N E Suzina, V N Polivtseva, T Z Esikova, D V Ross, V P Kholodenko, T N Abashina, V I Duda, A M Boronin

引用次数: 0

Endophytic Yeasts in Malus domestica and Pyrus communis Fruits under Anthropogenic Impact. 人为影响下家苹果和梨树果实内生酵母菌。

Mikrobiologiia Pub Date : 2017-01-01

A M Glushakova, A V Kachalkin

{"title":"Endophytic Yeasts in Malus domestica and Pyrus communis Fruits under Anthropogenic Impact.","authors":"A M Glushakova, A V Kachalkin","doi":"","DOIUrl":"","url":null,"abstract":"Yeast abundance and species diversity on the surface and in inner tissues of Malus domestica and\u0000Pyrus communis fruits under high anthropogenic impact in the city of Moscow (Russia) were studied. Results\u0000demonstrated that abundance of epiphytic yeasts on the fruits increased gradually, reaching the maximum of\u00003.2 × 10⁴ CFU/g on mature fruits. During summer, abundance of endophytes did not change significantly\u0000(variation near 2.5 × 10³ CFU/g) until complete maturation, while in September their numbers increased to\u0000104 CFU/g. Basidiomycetous yeasts (Filobasidium wieringae, F. magnum, Rhodotorula glutinis, and Rhodosporidiobolus\u0000colostri) predominated on the fruit surface. Ascomycetous species were the most diverse group\u0000inside the fruits, which quantitatively increased through maturation. It was found that the share of opportunistic\u0000species Candida parapsilosis in internal tissues was significant during the entire period of fruit formation\u0000and development under anthropogenic impact in the city. Specific properties of epiphytic and endophytic\u0000yeast communities developing in natural ecological niches under synanthropic conditions and anthropogenic\u0000impact are discussed.","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36483955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Intestinal Microbiota: Role in Development and Functioning of the Nervous System. 人类肠道微生物群:在神经系统发育和功能中的作用。

Mikrobiologiia Pub Date : 2017-01-01

O V Averina, V N Danilenko

{"title":"Human Intestinal Microbiota: Role in Development and Functioning of the Nervous System.","authors":"O V Averina, V N Danilenko","doi":"","DOIUrl":"","url":null,"abstract":"Recent results related to investigation of the role of intestinal microbiota (IM) in development and\u0000functioning of the human nervous system are discussed. The role of the microbiota in bidirectional communication\u0000between the gastrointestinal tract and the central nervous system is considered. Special attention is\u0000paid to the primary IM of infants, which is actively involved in formation of immune and other physiological\u0000mechanisms, including the nervous system, and is responsible for the subsequent general and psychical\u0000health of a human. The results of research on ability of the commensal intestinal microflora to produce neuroactive\u0000compounds, including neurotransmitters, short- and long-chain fatty acids, γ-aminobutyric acid,\u0000etc., are summarized. These compounds may have a considerable effect on development and functioning of\u0000the central nervous system, including the brain. Research on various animal models is discussed, including\u0000investigation of IM effect on behavior, learning abilities and memory, anxiety and depression levels, reaction\u0000to emotional stimuli, and stress resistance. A special section deals with probiotic bacteria, which are presently\u0000considered as psychobiotics with preventive and therapeutic potential for treatment of neurological and neurophysiological\u0000disorders. Development of new paradigms and concepts, rejection of some classical concepts\u0000of neurobiology is presently the key condition for the future breakthrough in investigation of human nervous\u0000activity.","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36484722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epiphytic microorganisms degrading aromatic hydrocarbons from the phyllosphere of urban woody plants. 城市木本植物层圈中降解芳烃的附生微生物。

Mikrobiologiia Pub Date : 2017-01-01

O I Sazonova, S L Sokolov, N V Prisyazhnaya, T Yu Izmalkova, I A Kosheleva, A M Boronin

引用次数: 0

Characterization of the lipopolysaccharide of Escherichia coli 126. 大肠杆菌126脂多糖的鉴定

Mikrobiologiia Pub Date : 2017-01-01

L D Varbanets, E A Zdorovenko, O S Brovarskaya, S V Kalinichenko

{"title":"Characterization of the lipopolysaccharide of Escherichia coli 126.","authors":"L D Varbanets, E A Zdorovenko, O S Brovarskaya, S V Kalinichenko","doi":"","DOIUrl":"","url":null,"abstract":"The lipopolysaccharide (LPS) of Escherichia coli 126 was isolated and studied. The lipid A fatty acid composition of the investigated LPS was similar to that of other members of the family Enterobacteriaceae. The E. coli 126 LPS was more toxic than the LPSs of previously studied E. coli strains and of other members of the Enterobacteriaceae (Budvicia aquatica and Pragia fontium), and was less pyrogenic than pyrogenal. SDS-PAG electrophoresis showed a bimodal distribution typical of S-form LPSs. The LPS of E. coli 126 decreased the adhesive index indicating a possible competition between LPS molecules of E. coli 126 and adhesins of E. coli F-50 on rabbit erythrocytes. The LPS of E. coli 126 in a homologous system showed antigenic activity in the reactions of double immunodiffusion in agar by Ouchterlony. No serological cross-reaction of the LPS of other E. coli strains, as well as of that of the B. aquatica type strain, with the antiserum to E. coli 126 was observed. The structural components of the lipopolysaccharide obtained by mild acid hydrolysis were lipid A, the core oligosaccharide, and the O-specific polysaccharide. Based on the data of monosaccharide analysis and 1H and 13C NMR spectroscopy it was found that the O-specific polysaccharide had the structure characteristic of the representatives of E. coli serogroup O15.","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36484727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel Delftia plant symbiont capable of autotrophic methylotrophy. 一种能自养甲基化的Delftia植物共生体。

Mikrobiologiia Pub Date : 2017-01-01

N V Agafonova, N V Doronina, E N Kaparullina, D N Fedorov, A B Gafarov, O I Sazonova, S L Sokolov, Yu A Trotsenko

{"title":"A novel Delftia plant symbiont capable of autotrophic methylotrophy.","authors":"N V Agafonova, N V Doronina, E N Kaparullina, D N Fedorov, A B Gafarov, O I Sazonova, S L Sokolov, Yu A Trotsenko","doi":"","DOIUrl":"","url":null,"abstract":"A facultative methylotrophic bacterium, strain Lp-1, which was isolated from root nodules of lupine (Lupinus polyphyllus L.) on the medium with methanol as a carbon and energy source, exhibited high similarity of the 16S rRNA gene sequences to Delftia strains (94‒99.9%). The cells of Delftia sp. Lp-1 were motile gram-negative rods dividing by binary fission. Predominant fatty acids were C16:0 (34.2%), C16:1ω9 (14.5%), and C18:1ω7c (17.3%). Phosphatidylethanolamine, phosphatidylcholine, and phosphatidylglycerol were the dominant phospholipids. Q8 was the major ubiquinone. Optimal growth occurred at 24‒26°C and pH 7.1‒7.3; growth was inhibited by 1% NaCl. The organism oxidized methanol with the classical methanol dehydrogenase and used the ribulose bisphosphate pathway of C1 metabolism. Analysis of translated amino acid sequence of the large subunit of the MxaF methanol dehydrogenase revealed 85.5‒94% similarity to the sequences of such autotrophic methylotrophs of the class Alphaproteobacteria as Angulomicrobium, Starkeya, and Ancylobacter, indicating the possible acquisition of the mxaF gene via horizontal gene transfer. Delftia sp. Lp-1 (VKM B-3039, DSM 24446), the first methylotrophic member of the genus Delftia, was shown to be a plant symbiont, stimulating plant growth and morphogenesis, increasing the level of photosynthetic pigments and specific leaf weight. It possesses the nifH gene of nitrogen fixation, is capable of phosphate solubilization, synthesis of auxins and siderophores, and is antagonistic to plant pathogenic fungi and bacilli.","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36485138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and Seasonal Trophodynamics of Picophytoplankton in Sevastopol Bay and Adjacent Waters (the Black Sea). 黑海塞瓦斯托波尔湾及其邻近水域浮游植物的结构和季节营养动力学。

Mikrobiologiia Pub Date : 2016-09-01

V S Mukhanovi, O A Rylkova, T Ya Churiloval, E G Sakhon, N V Pimenov

{"title":"Structure and Seasonal Trophodynamics of Picophytoplankton in Sevastopol Bay and Adjacent Waters (the Black Sea).","authors":"V S Mukhanovi, O A Rylkova, T Ya Churiloval, E G Sakhon, N V Pimenov","doi":"","DOIUrl":"","url":null,"abstract":"Abundance and seasonal trophodynamics. (specific growth rate, daily production, and grazing mortality) of the major picophytoplankton components, Synechococcus cyanobacteria (Syn) and picoeukary- otes (Pico-E), were studied at three stations in Sevastopol Bay and adjacent coastal waters (the Black Sea) in 2014 by flow cytometry and the dilution method. Pico- E abundance was shown to increase along the nutrient and pollution gradient from the coastal waters outside the bay (annual average of 7.3 ± 5.4 x 103 cells mL⁻¹) to the eastern corner of the bay (28.7 ± 11.4 x 103 cells mL⁻¹), while no relation was found between the water pollution status.and Syn abundance (9.9 ± 8.7 x 10³ cells mL⁻¹, at all the stations, n=27). Matter flows through the communities (daily production for Syn and Pico-E 0-16.6 and 0-19.3 μg C L- day⁻¹, respec- tively; grazing mortality for Syn and PicoE 0-3.6 and 0-21.2 μg C L⁻¹ day⁻¹, respectively) were comparable to or even exceeded their biomass stocks (<0.05-6.8 and 0.9-26.5 μg C L- for Syn and PicoE, respectively), indicating high biomass turnover rates. The highest flow-to-stock ratio (up to 6 for Syn) and,a significant imbalance between daily production (P) and grazing mortality (G) were observed in the most polluted and eu- trophicated waters of the bay in spring (Pico-E: P/G <.1) and late summer (Syn: P/G > 1). Black River inflow to the bay was hypothesized to be among the mechanisms maintaining.this pronounced and long-term im- balance in the open system without any negative consequences for the picophytoplankton assemlages.","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35762583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Role of the Microbial Community in Formation of Speleothem (Moonmilk) in the Snezhnaya Carst Cave (Abkhazia).] [微生物群落在Snezhnaya Carst Cave (Abkhazia) Speleothem (Moonmilk)形成中的作用]

Mikrobiologiia Pub Date : 2016-09-01

引用次数: 0