Mikrobiologiia最新文献_第10页

[Towards Reinstatement of the Yeast Genus Zygowilliopsis Kudriavzev (1960)]. [关于恢复酵母属zygowillopsis Kudriavzev(1960)]。

Mikrobiologiia Pub Date : 2015-09-01

G I Naumov, E S Naumova, Ch Fu Lee

引用次数: 0

[Formation of 55-kDa Fragments under Impaired Coordination Bonds and Hydrophobic Interactions in Peripheral Light-Harvesting Complexes Isolated from Photosynthetic Purple Bacteria]. [从光合紫色细菌中分离的外周光收集复合物在受损配位键和疏水相互作用下形成55-kDa片段]。

Mikrobiologiia Pub Date : 2015-05-01

A A Solov'ev, Y E Erokhin

{"title":"[Formation of 55-kDa Fragments under Impaired Coordination Bonds and Hydrophobic Interactions in Peripheral Light-Harvesting Complexes Isolated from Photosynthetic Purple Bacteria].","authors":"A A Solov'ev, Y E Erokhin","doi":"","DOIUrl":"","url":null,"abstract":"Size exclusion chromatography was used to assess the relative size of intact and diphenylamine-treated (DPA, with suppressed carotenoid synthesis) peripheral light-harvesting complexes (LH2 complexes) of the sulfurbacterium Allochromatium minutissimum. Both LH2 complexes were nonamers and had the same elution volume V(e), coinciding with that for the LH2 complex of Rhodoblastus acidophilus (strain 10050). Their molecular mass was 150 kDa. Bot pheophytinization of bacteriochlorophyll (BChl) at low pH and treatment with the detergent LDAO, affecting the hydrophobic interactions between the neighboring protomers, result in the fragmentation of the ring of the isolated LH2 complexes and formation of 55-kDa fragments with molecular masses corresponding to one-third of the initial value. Fragmentation caused by both pheophytinization and detergent treatment was much more rapid in DPA-treated LH2 complexes than in the intact ones. The 55-kDa fragments formed at low pH values contained monomeric bacteriopheophytin, while the fragments of a similar molecular mass formed at pH 8.0 in the presence of the detergent contained monomeric BChl. The observed fragmentation was hypothesized to reflect the inherent C3 symmetry of the LH2 complexes, with the preliminarily assembled trimers used as building blocks.","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33981053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Physiological Properties of Acidithiobacillus ferrooxidans Strains Isolated from Sulfide Ore Deposits in Kazakhstan]. [哈萨克斯坦硫化物矿床中酸性氧化亚铁硫杆菌菌株的生理特性]。

Mikrobiologiia Pub Date : 2015-05-01

Z K Kanaeva, A G Bulaev, A T Kanaev, T F Kondrat'eva

{"title":"[Physiological Properties of Acidithiobacillus ferrooxidans Strains Isolated from Sulfide Ore Deposits in Kazakhstan].","authors":"Z K Kanaeva, A G Bulaev, A T Kanaev, T F Kondrat'eva","doi":"","DOIUrl":"","url":null,"abstract":"Acidithiobacillus ferroxidans strains were isolated from acidophilic microbial communities of Kazakhstan sulfide ore deposits. Their biotechnologically important properties (optimal and maximal growth temperatures and resistance to NaCl) were determined. While temperature optima of the strains were the same (30-32 degrees C), temperature ranges were different. Thus, strain TFBK oxidized iron very poorly at 37 degrees C, while for strain TFV, the iron oxidation rate at this temperature was insignificantly lower than at lesser temperatures. NaCl inhibited the oxidative activity of both strains. Iron oxidation by strain TFV was inhibited at 5 g/L NaCl and was suppressed almost completely at 20 g/L. Iron oxidation by strain TFBK was inhibited by NaCl to a lesser degree, so that iron oxidation rate was relatively high at 10 g/L, while at 20 g/L NaCl the process was not suppressed completely, although the oxidation rate was low. Sulfur oxidation by these strains was less affected by NaCl than oxidation of ferrous iron. Sulfur oxidation by strain TFV was considerably inhibited only at 20 g/L NaCl, but was not suppressed completely. Sulfur oxidation by strain TFBK was more affected by NaCl. At 10 g/L NaCl the oxidation rate was much lower than at lower NaCl concentrations (sulfate concentrations after 6 days of oxidation at 5 and 10 g/L NaCl were -130 and -100 mM, respectively). While sulfur oxidation by strain TFBK was considerably inhibited at 10 and 20 g/L NaCl, similar to strain TFV it was not suppressed completely. Our results indicate the adaptation of the species A. ferrooxidans to a broad range of growth conditions.","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34084121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Yeasts of the Vineyards in Dagestan and Other Regions]. [达吉斯坦和其他地区葡萄园的酵母]。

Mikrobiologiia Pub Date : 2015-05-01

A V Kachalkin, D A Abdullabekova, E S Magomedova, G G Magomedov, I Yu Chernov

引用次数: 0

[Resistance of Various Yeast Ecological Groups to Prolonged Storage in Dry State]. 不同酵母生态类群对长期干燥贮藏的抗性。

Mikrobiologiia Pub Date : 2015-05-01

A M Glushakova, A V Kachalkin, T M Zheltikova, I Yu Chernov

引用次数: 0

[Active Sulfate Reduction in Acidic Sediments of Gold Mine Tailings]. [金矿尾矿酸性沉积物活性硫酸盐还原研究]。

Mikrobiologiia Pub Date : 2015-05-01

N V Pimenov, D A Ivasenko, A L Gerasimchuk, E E Zakharova, A V Mardanov, O V Karnachuk

引用次数: 0

[Identification of Hydrocarbon-Oxidizing Dietzia Bacteria from Petroleum Reservoirs Based on Phenotypic Properties and Analysis of the 16S rRNA and gyrB Genes]. [基于表型特性及16S rRNA和gyrB基因分析的油气氧化Dietzia细菌鉴定]。

Mikrobiologiia Pub Date : 2015-05-01

T N Nazina, E S Shumkova, D Sh Sokolova, T L Babich, M V Zhurina, Yan-Fen Xue, G A Osipov, A B Poltaraus, T P Tourova

{"title":"[Identification of Hydrocarbon-Oxidizing Dietzia Bacteria from Petroleum Reservoirs Based on Phenotypic Properties and Analysis of the 16S rRNA and gyrB Genes].","authors":"T N Nazina, E S Shumkova, D Sh Sokolova, T L Babich, M V Zhurina, Yan-Fen Xue, G A Osipov, A B Poltaraus, T P Tourova","doi":"","DOIUrl":"","url":null,"abstract":"The taxonomic position of hydrocarbon-oxidizing bacterial strains 263 and 32d isolated from formation water of the Daqing petroleum reservoir (PRC) was determined by polyphasic taxonomy techniques, including analysis of the 16S rRNA and the gyrB genes. The major chemotaxonomic characteristics of both strains, including the IV type cell wall, composition of cell wall fatty acids, mycolic acids, and menaquinones, agreed with those typical of Dietzia strains. The DNA G+C content of strains 263 and 32d were 67.8 and 67.6 mol%, respectively. Phylogenetic analysis of the 16S rRNA gene of strain 32d revealed 99.7% similarity to the gene of D. maris, making it possible to identify strain 32d as belonging to this species. The 16S rRNA gene sequence of strain 263 exhibited 99.7 and 99.9% similarity to those of D. natronolimnaea and D. cercidiphylli YIM65002(T), respectively. Analysis of the gyrB genes of the subterranean isolates and of a number of Dietzia type strains confirmed classiffication of strain 32d as a D. maris strain and of strain 263, as a D. natronolimnaea strain. A conclusion was made concerning higher resolving power of phylogenetic analysis of the gyrB gene compared to the 16S rRNA gene analysis in the case of determination of the species position of Dietzia isolates.","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33981061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Root Nodule Bacteria Sinorhizobium meliloti: Tolerance to Salinity and Bacterial Genetic Determinants]. 根瘤细菌Sinorhizobium meliloti:耐盐性和细菌遗传决定因素。

Mikrobiologiia Pub Date : 2015-05-01

M L Roumiantseva, V S Muntyan

{"title":"[Root Nodule Bacteria Sinorhizobium meliloti: Tolerance to Salinity and Bacterial Genetic Determinants].","authors":"M L Roumiantseva, V S Muntyan","doi":"","DOIUrl":"","url":null,"abstract":"The theoretical and experimental data on salt tolerance of root nodule bacteria Sinorhizobium meliloti (Ensifer meliloti), an alfalfa symbiont, and on genetic determination of this feature are reviewed. Extensive data on the genes affecting adaptation of proteobacteria are provided, as well as on the groups of genes with activity depending on the osmolarity of the medium. Structural and functional polymorphism of the bet genes involved in betaine synthesis and transport in S. meliloti is discussed. The phenotypic and. genotypic polymorphism in 282 environmental rhizobial strains isolated from the centers of alfalfa diversity affected by aridity and salinity is discussed. The isolates from the Aral Sea area and northern Caucasus were shown to possess the betC gene represented by two types of alleles: the dominant A-type allele found in Rm 1021 and the less common divergent E-type allele, which was revealed in regions at the frequencies at the frequencies of 0.35 and 0.48, respectively. In the isolates with the salt-tolerant phenotype, which were isolated from root nodules and subsequently formed less effective symbioses with alfalfa, the frequency of E-type alleles was 2.5 times higher. Analysis of the nucleotide and amino acid sequences of the E-type allele of the betC gene revealed that establishment of this allele in the population was a result of positive selection. It is concluded that diversification of the functionally diverse bet genes occurring in S. meliloti affects the salt tolerance and symbiotic effectivity of rhizobia.","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34084117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Regulation of Biofilm Formation by Pseudomonas chlororaphis in an vitro System]. 绿假单胞菌在体外系统中生物膜形成的调控

Mikrobiologiia Pub Date : 2015-05-01

A V Gannesen, M V Zhurina, M A Veselova, I A Khmel, V K Plakunov

引用次数: 0

[Applicability of MALDI Mass Spectrometry for Diagnostics of Phase Variants in Bacterial Populations]. [MALDI质谱法诊断细菌种群相变的适用性]。

Mikrobiologiia Pub Date : 2015-05-01

N A Kryazhevskikh, N G Loiko, E V Demkina, A L Mulyukin, A T Lebedev, A M Gaponov, A V Tutel'yan, Yu A Nikolaeva, G I El'-Registan

{"title":"[Applicability of MALDI Mass Spectrometry for Diagnostics of Phase Variants in Bacterial Populations].","authors":"N A Kryazhevskikh, N G Loiko, E V Demkina, A L Mulyukin, A T Lebedev, A M Gaponov, A V Tutel'yan, Yu A Nikolaeva, G I El'-Registan","doi":"","DOIUrl":"","url":null,"abstract":"Efficiency of MALDI mass spectrometry for differentiation between phenotypic phase variants (in colony morphology and virulence/avirulence) was investigated.for saprotrophic and opportunistically pathogenic bacteria of five genera (Acinetobacter, Arthrobacter, Rhodococcus, Corynebacterium, and Escherichia). Analysis of MALDI spectra (on the SA and HCCA matrices) included: (1) determination of similarity of the protein spectra as a percentage of the common protein peaks to the total amount of proteins, which reflects the phylogenetic relationships of the objects and has been recommended for identification of closely related species; (2) comparison of intensities of the common peaks; and (3) the presence of specific peaks as determinative characteristics of the variants. Under the standard analytical conditions the similarity between the MALDI profiles was shown to increase in the row: genus-species-strain-variant. Assessment of intensities of the common peaks was most applicable for differentiation between phase variants, especially in the case of high similarity of their profiles. Phase variants (A. oxydans strain K14) with similar colony morphotypes (S, R, M, and S(m)) grown on different media (LB agar, TSA, and TGYg) exhibited differences in their protein profiles reflecting the differences in their physiological characteristics. This finding is in agreement with our previous results on screening of the R. opacus with similar colony morphology and different substrate specificity in decomposition of chlorinated phenols. Analysis of MALDI spectra is probably the only efficient method for detection of such variants.","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34084119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0