Minerva dental and oral science最新文献

{"title":"Diagnosis of odontogenic keratocysts and non-keratocysts using edge attention convolution neural network.","authors":"Nivedan Yakolli, Divya B Shivanna, Roopa S Rao, Shankargouda Patil, Vincenzo Ronsivalle, Marco Cicciù, Giuseppe Minervini","doi":"10.23736/S2724-6329.24.04874-5","DOIUrl":"10.23736/S2724-6329.24.04874-5","url":null,"abstract":"<p><strong>Background: </strong>The study's objective was to develop an automated method for a histopathology recognition model for odontogenic keratocysts (OKC) and non-keratocyst (Non-KC) in jaw cyst sections stained with hematoxylin (H) and eosin (E) on a tiny bit of incisional biopsy prior to surgery.</p><p><strong>Methods: </strong>This hastens the speed and precision of diagnosis to patients. Also, navigates the clinicians with the therapeutic doctrine. To build such a system and to increase the accuracy of the existing models, the edge attention CNN model with Keras functional API was implemented which efficiently analyzes the texture information of the images. Approximately 2861 microscopic images at a 40X magnification were taken from 54 OKC, 23 Dentigerous cysts (DC), and 20 Radicular cysts.</p><p><strong>Results: </strong>The model was trained using both RGB and canny edge-detected images. The model gave a good accuracy of 96.8%, which is suitable for real-time. Histopathological images are better analyzed through textural features. The proposed edge attention CNN highlights the edges, making texture analysis more precise.</p><p><strong>Conclusions: </strong>The suggested method will work for OKC and Non-KC diagnosis automation systems. The use of a whole slide imaging scanner has the potential to increase accuracy and remove human bias.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":" ","pages":"303-311"},"PeriodicalIF":1.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142350247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in-vivo study on the effect of head orientation in the measurement of anthropometric points in stereophotogrammetry.","authors":"Marco Di Blasio, Giuseppe Minervini, Marzia Segù, Giuseppe Pedrazzi, Alberto Di Blasio, Diana Cassi, Marco Cicciù, Bendetta Vaienti","doi":"10.23736/S2724-6329.24.04852-6","DOIUrl":"10.23736/S2724-6329.24.04852-6","url":null,"abstract":"<p><strong>Background: </strong>It is well known that in bidimensional photography the orientation of the head is fundamental to obtain a correct reproduction of the subject. Even minimal errors in yaw, roll and pitch of the head can be cause of unreliability of the photography itself. This is not the case in three-dimensional photography in which head positioning may have no influence on the reliability of the results since on the computer the 3D reconstruction of the face can be oriented as desired, allowing any small errors to be corrected. The authors, in a previous in-vitro study, demonstrated this using a mannequin of the head. In the present study, the Authors repeat the study in vivo to verify any differences.</p><p><strong>Methods: </strong>Seventeen anthropometric points were marked on the face of a young adult female (26 years old) with eyeliner. Images of the face were then acquired with a stereo-photogrammetric system (Face Shape 3D MaxiLine) in natural head position and with varying degrees of roll, pitch and yaw. The anthropometric points marked on the skin were then recognized and marked on a digital software (Viewbox, dHAL Software, 6 Menandrou Street, Kifissia 14561, Greece) on each individual image. 14 linear distances between these points were measured using the same software.</p><p><strong>Results: </strong>The linear distances of these points in the stereophotos taken at centered positions were then compared with those taken with different degrees of roll, pitch and yaw. This study confirms previous results obtained in vitro regarding the presence of areas of non-equivalence (P<0.05) when comparing the frontal and lateral regions of the face at various angles.</p><p><strong>Conclusions: </strong>However, it introduces important suggestions on the collection of data from specific anthropometric points. If these points are to be assessed, the medical staff will have to take special precautions to obtain reliable images.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":" ","pages":"343-351"},"PeriodicalIF":1.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical considerations of chemical structure and biosafety of current ceramic biomaterials for dentistry: review and outlook of the current state of the art.","authors":"Gennaro Musella, Andrea Ballini, Michele Di Cosola, Alessandro Motta, Tommaso Dai Prà, Fariba Esperouz, Mario Dioguardi, Lorenzo Lo Muzio, Gaetano Illuzzi, Maria E Bizzoca","doi":"10.23736/S2724-6329.24.05093-9","DOIUrl":"10.23736/S2724-6329.24.05093-9","url":null,"abstract":"<p><p>Biomaterial refers to any material, non-pharmaceutical or combination of substances of synthetic or natural origin used for any period independently or as part of a system for the purpose of healing, assisting the growth or replacement of tissues, organs or functions of the body. It is an unsustainable material that interacts with biological systems. Before being produced a biomaterial goes through a sequence of steps, which includes: 1) the definition of the problem that the material will be called to solve (treatment, replacement of the instrument, aesthetic reasons); 2) design of the device, composition and control of materials (mechanical properties, toxicity, biological response, corrosion resistance, interaction with proteins, cells and tissues), manufacture, sterilization and standardization; 3) control of the device (in experimental animals, in vitro) clinical studies, studies; and 4) monitor its long-term use in vivo, in vitro and in patients. Research for ceramic materials is evolving and because they can be porous or glassy, they find applications in medicine and biotechnology, as they can be used as fillers, covering materials and scaffolds. Therefore, ceramic biomaterials are widely used not only in the field of orthopedic and maxillofacial surgery but also in dentistry as for dental prostheses. Materials monitoring technologies allow us to monitor the three-dimensional development in space, as well as imperfections or micro-cracks in the ceramic.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":" ","pages":"352-358"},"PeriodicalIF":1.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effect of aging on the shear bond strength between old composite resin restoration and the repair composite resin: an in-vitro study.","authors":"Hafiz A Adawi, Saurabh Jain, Ahmed O Shami, Hamood M Mahdali, Ali M Kaabi, Osama B Balki, Marco Cicciù, Giuseppe Minervini","doi":"10.23736/S2724-6329.24.04889-7","DOIUrl":"https://doi.org/10.23736/S2724-6329.24.04889-7","url":null,"abstract":"<p><strong>Background: </strong>The current study aimed to evaluate the effect of aging on the repair bond strength between old composite resin and repaired composite resin and to evaluate which type of repair composite material provides better bond strength in different time durations.</p><p><strong>Methods: </strong>Eighty nano-hybrid composite specimens were randomly divided into four groups (N.=20) and exposed to accelerated aging from 0 to 2 years. Each group was further divided into two subgroups, A and B, repaired with packable and flowable composite respectively, after micro-abrasion treatment. Shear bond strength was assessed and statistically analyzed.</p><p><strong>Results: </strong>It was observed that with an increase in aging time, the bond strength of both the composite materials used for repair decreased. The flowable composite's mean bond strength (22.09±0.65, 18.74±2.36, and 17.74±2.38 MPa) was more than the packable composite (21.97±0.84, 17.97±3.85, and 15.97±2.45 MPa) used for repair on aged specimens. In contrast, the bond strength of the packable was more than the flowable composite in control group specimens that were not aged (23.25±0.89 vs. 22.40±1.26).</p><p><strong>Conclusions: </strong>As restored composites age, their bond strength decreases. Flowable composite displays better repair shear strength than packable composite as aging progresses. Micro abrasion followed by flowable composite bonding can repair aged composite restorations effectively.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-based prediction and classification of root caries using radiographic images.","authors":"Pradeep K Yadalam, Jeevitha Manickavasagam, Trisha Sasikumar, Maria M Marrapodi, Vincenzo Ronsivalle, Marco Cicciù, Giuseppe Minervini","doi":"10.23736/S2724-6329.24.04967-2","DOIUrl":"https://doi.org/10.23736/S2724-6329.24.04967-2","url":null,"abstract":"<p><strong>Background: </strong>Root surface caries, commonly known as root decay, is a common dental disorder that affects tooth roots. Like enamel-based tooth decay, root caries attack exposed root surfaces caused by gum recession or periodontal disease. Older persons with gum recession, tooth loss, or poor oral hygiene may be more likely to develop this disorder. Dental root caries must be diagnosed early to improve treatment and prevention. This research will examine radiographic image-based AI-based root caries prediction algorithms.</p><p><strong>Methods: </strong>Saveetha Dental College supplied 200 root surface radiographs. An expert dentist and dental radiologist confirmed one hundred teeth with root caries and 100 without. Edited and segmented radiographic images. Orange, a machine learning squeeze net embedding model with Naive Bayes, Logistic Regression, and neural networks, was used to assess prediction accuracy. Training and test data were split 80/20. Cross-validation, confusion matrix, and ROC analysis assessed model performance. This study examined precision and recall.</p><p><strong>Results: </strong>Naïve bayes and logistic regression have 96% and 100% accuracy, but class accuracy is -94% and 100% in image classification of root caries was seen.</p><p><strong>Conclusions: </strong>AI-based root caries prediction utilizing radiographic images would improve dental care by diagnosing and treating early, accurately, and personalized. With appropriate deployment, research, and ethics, AI integration in dentistry could benefit practitioners and patients. Dental professionals and AI experts must work together to maximize this new technology.AI integration in dentistry can significantly improve root caries diagnosis and treatment by predicting root caries using radiographic images. This early detection reduces treatment need and time. Collaboration between dental professionals and AI experts is crucial for maximizing benefits.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of red blood cell parameters for signs of iron deficiency anemia in patients with oral diseases.","authors":"Kavithaa Rajasekaran, Kaviyarasi Renu, Kavitha Sankaran, Vishnu Priya Veeraraghavan, Gayathri Rengasamy, Vincenzo Ronsivalle, Marco Cicciù, Giuseppe Minervini","doi":"10.23736/S2724-6329.24.04907-6","DOIUrl":"https://doi.org/10.23736/S2724-6329.24.04907-6","url":null,"abstract":"<p><strong>Background: </strong>A lack of healthy red blood cells such as iron deficiency frequently consequences as anemia. In expectant women, iron deficiency increases the risk of developmental delays in the fetus. It is utilized to regenerate red blood cells (RBC), which determines the storage and transport of oxygen in the body. As for oral symptoms and indications, it has an inclusive of atrophic glossitis, oral mucosa atrophy, and oral mucosa tenderness or a sensation with burning in mouth. The objective is to determine RBC parameters for iron deficiency anemia (IDA) symptoms in oral disease patients.</p><p><strong>Methods: </strong>Normal and oral disease patients' blood is collected in a 2-mL EDTA vial for the hematology analyzer. With file number SRB/SDC/UG-2155/22/493, the institutional ethical approval committee approved this study: 10 controls (without anemia) and 10 patients with oral disease (with anemia).</p><p><strong>Results: </strong>In oral disease patients with anemia, a decreased hemoglobin level is observed. In this study, nearly all anemic patients have IDA. In IDA, the RBC count decreases, the MCV decreases, the MCH decreases, and the RDW rises. We have analyzed various iron deficiency anemia indices. Ricercari and Eshani demonstrate a significant change in it.</p><p><strong>Conclusions: </strong>In predicting iron deficiency anemia, our results indicate that oral patients have significant differences in hematological parameters that are individual. It demonstrates that oral disease patients are developing iron deficiency anemia than controls (healthy). Additionally, the discrimination indices reveal that they do not have hemoglobinopathies but rather iron deficiencies. Oral manifestations are more prevalent in Iron deficiency anemia (IDA) patients than in healthy patients, according to our findings.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pyogenic granuloma: localization, incidence, clinical features.","authors":"Afrah A Aldelaimi, Hamid H Enezei, Thamer Y Humadi, Khalil A Mohammed, Tahrir N Aldelaimi, Maria M Marrapodi, Gabriele Cervino, Giuseppe Minervini","doi":"10.23736/S2724-6329.24.05075-7","DOIUrl":"https://doi.org/10.23736/S2724-6329.24.05075-7","url":null,"abstract":"<p><strong>Background: </strong>A pyogenic granuloma (PG) represents a swiftly proliferating benign vascular tumor situated in the skin or subcutaneous tissue, commonly observed as a reactive lesion in the orofacial area. This research intended to scrutinize the clinicopathological characteristics of pyogenic granulomas in the oral and maxillofacial region.</p><p><strong>Methods: </strong>Seventy two participants, comprising 24 men (33%) and 48 women (67%), underwent surgical ablation of pyogenic granuloma in oral and maxillofacial regions utilizing 940nm and 980nm diode lasers. Standard tissue processing procedures were applied to all specimens, with subsequent slicing of paraffin-embedded blocks at a 5µm thickness for H & E staining. A microscopic examination via light microscopy was conducted on all slides to confirm the diagnosis.</p><p><strong>Results: </strong>All cases underwent pyogenic granuloma surgical laser ablation, with 53 individuals (74%) aged 30 years or younger, 19 (26%) aged 30 years or more, 52 (72%) have duration less than six months,, and 20 (28%) have a six months or more.</p><p><strong>Conclusions: </strong>Pyogenic granuloma emerges as a prevalent reactive lesion in the oral and maxillofacial domain. The diode laser demonstrates utility in oral and facial soft tissue surgery owing to its facile application, enhanced coagulation, avoidance of suturing, reduced swelling and pain, and its capacity for esthetically favorable outcomes, encompassing superior de-epithelialization, absence of bleeding, and improved repair. The roles of surgeons and pathologists are pivotal in diagnosing and treating pyogenic granuloma in oral and maxillofacial regions.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142676356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-silico immunoinformatic vaccine design for Treponema denticola ergothionase.","authors":"Jai P Rexlin, Jeevitha Manickavasagam, Pradeep K Yadalam, Deepti Shrivastava, Kumar C Srivastava, Vincenzo Ronsivalle, Marco Cicciù, Giuseppe Minervini","doi":"10.23736/S2724-6329.24.04915-5","DOIUrl":"https://doi.org/10.23736/S2724-6329.24.04915-5","url":null,"abstract":"<p><strong>Background: </strong>Treponema denticola, a well-studied oral spirochete, adheres, invades, and damages periodontal tissues - gram-negative, anaerobic Treponema denticola. In previous research, sub-gingival spirochetes have correlated positively with dental plaque score, pocket, and clinical attachment level measurements. Hence, the study aims to design an immunoinformatic vaccine using a reverse vaccinology approach against Treponema denticola ergothionase.</p><p><strong>Methods: </strong>Protein Data Bank provided the FASTA amino acid sequence of Treponema denticola. Antigenicity, toxicity, and stability of discovered T-cell epitopes were evaluated to develop 6S7Q B and A multiepitope vaccination design. The Vaccine's dual major histocompatibility complex (MHC I and II) binding epitopes were also predicted. The designed Vaccine's identified epitope sequence and secondary structure were then predicted and validated. Protein-protein interactions involving ergothionase and human beta-defensins were investigated using molecular docking.</p><p><strong>Results: </strong>The designed Vaccine had high antigenicity, toxicity, and stability. The Vaccine's three-dimensional structure demonstrated a significant association with beta-defensin. Its low binding energy score of -827.6 kcal/mol indicates that the immune system will respond favorably to the antigen.</p><p><strong>Conclusions: </strong>In this research, we employed immunoinformatic techniques to create a reverse vaccination effort to develop an in-silico vaccine.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene expression analysis of microRNA-1285 in the South Indian oral squamous cell carcinoma population.","authors":"Mahathi Neralla, Hiranya S, Sushmaa C Selvakumar, K Auxzilia Preethi, Durairaj Sekar","doi":"10.23736/S2724-6329.23.04798-8","DOIUrl":"10.23736/S2724-6329.23.04798-8","url":null,"abstract":"<p><strong>Background: </strong>Oral squamous cell carcinoma (OSCC) is the most common oral malignant tumor, which has poor prognosis. The traditional investigative modality is invasive biopsy which is the gold standard for diagnosis. In recent years, alternative methods like non-invasive biomarkers have been studied for their potential role in early diagnosis and prognosis. Among them, microRNAs (miRNAs or miRs) are short non-coding RNAs that regulate gene expression in various diseases, including OSCC. Several miRNAs are being researched as non-invasive biomarkers as well as novel therapeutic targets in the treatment of OSCC. MiR expression can be upregulated or downregulated in OSCC. Among the reported miRNAs, miR-1285 is an important miRNA found to be involved in OSCC. The aim of the current study was to quantify the levels of miR-1285 in OSCC samples and to validate their potential role as biomarkers for OSCC detection.</p><p><strong>Methods: </strong>Sixteen samples of cancer tissue and normal tissue were evaluated from a total of 25 patients, in the study, conducted in the Department of Oral and Maxillofacial Surgery. The tissues were processed for H&E staining and gene expression analysis of miR-1285. The samples were collected after proper informed consent from the patients. Total RNA isolated was reverse transcribed into cDNA which was used in the gene expression analysis using qRT-PCR.</p><p><strong>Results: </strong>The histopathological examination confirmed the OSCC cases and the gene expression analysis revealed that miR-1285 was significantly downregulated in OSCC tissues. Since miR-1285 showed significant difference between the OSCC and normal tissues it could be postulated as a potential biomarker and therapeutic target for OSCC.</p><p><strong>Conclusions: </strong>Further in-vitro and in-vivo studies could validate their functional role in OSCC.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":" ","pages":"249-255"},"PeriodicalIF":1.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9812581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-34a-3p and its target tumor necrosis factor-α in the regulation of South Indian oral squamous cell carcinoma population.","authors":"M S Varshan, Sushmaa C Selvakumar, K A Preethi, Karthikeyan Murthykumar, Dhanraj M Ganapathy, Durairaj Sekar","doi":"10.23736/S2724-6329.23.04835-0","DOIUrl":"10.23736/S2724-6329.23.04835-0","url":null,"abstract":"<p><strong>Background: </strong>The most frequent head and neck cancer is oral squamous cell carcinoma (OSCC), the common histological cancer of the oral cavity and is one of the most prevalent forms of cancer globally. It has been known that there are several biomarkers and therapeutic targets that have been discovered for OSCC, but none of them were effective against the progression of OSCC. Interestingly, small non-coding RNAs termed microRNAs (miRNAs) regulate cellular activity by targeting numerous signaling pathways or genes that either promote or repress the progression of diseases. Surprisingly, among the differentially expressed miRNAs, miR-34a was identified to be highly sensitive and specific to OSCC and widely studied for its role in various cancers, including OSCC.</p><p><strong>Methods: </strong>The secondary structure of miR-34a-3p was analyzed using bioinformatic analysis and its targets were screened using the TargetScan database. Specimens of 25 OSCC cancer tissues and adjacent normal tissues were collected from the Department of Oral and Maxillofacial Surgery, Saveetha Dental College and Hospitals. The tissues were processed for H&E staining and gene expression analysis of miR-34a-3p and tumor necrosis factor alpha (TNF-α).</p><p><strong>Results: </strong>The minimum free energy for miR-34a-3p was found to be -47.20 kCal which proved the stability of the miRNA. The histopathological examination confirmed the OSCC cases and the gene expression analysis revealed that miR-34a-3p was significantly downregulated in OSCC tissues, whereas TNF-α showed vice versa expression.</p><p><strong>Conclusions: </strong>miR-34a-3p could be postulated as a potential therapeutic target for OSCC.</p>","PeriodicalId":18709,"journal":{"name":"Minerva dental and oral science","volume":" ","pages":"256-263"},"PeriodicalIF":1.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50158338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}