Huiying Huang, Shuxin Du, Lei Guo, Mengli Zhou, Xinzhu Zhao, Fanhao Cui, Ruihong Wang
{"title":"Chloroplast genome characteristics and phylogenetic analysis of <i>Scrophularia marilandica</i> Linnaeus (Scrophulariaceae).","authors":"Huiying Huang, Shuxin Du, Lei Guo, Mengli Zhou, Xinzhu Zhao, Fanhao Cui, Ruihong Wang","doi":"10.1080/23802359.2025.2555458","DOIUrl":"10.1080/23802359.2025.2555458","url":null,"abstract":"<p><p><i>Scrophularia marilandica</i> Linnaeus 1753 is a herbaceous perennial medicinal plant of the family Scrophulariaceae, native throughout eastern and central North America. In this study, the first complete chloroplast genome of <i>S. marilandica</i> was reported and phylogenetic analysis was conducted with other 11 species from Scrophulariaceae. The chloroplast genome was 152,414 bp with 132 genes and includes a large single-copy (LSC) region (83,583 bp), a small single-copy (SSC) region (17,925 bp), and a pair of inverted repeat (IRs) regions (25,453 bp). The chloroplast genome reported here will be beneficial for its interspecific identification and evolutionary studies of <i>Scrophularia.</i></p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 10","pages":"914-918"},"PeriodicalIF":0.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12409920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhi-Peng Chen, Wen-Jian Li, Hong-Xia Hou, Guo-Hao Zu
{"title":"The complete mitochondrial genome of <i>Leptomastidea bifasciata</i> (Hymenoptera: Chalcidoidea: Encyrtidae) and phylogenetic analysis.","authors":"Zhi-Peng Chen, Wen-Jian Li, Hong-Xia Hou, Guo-Hao Zu","doi":"10.1080/23802359.2025.2555459","DOIUrl":"10.1080/23802359.2025.2555459","url":null,"abstract":"<p><p><i>Leptomastidea</i> (Hymenoptera, Encyrtidae) are parasitoids of agricultural/forestry mealybug pests. This study first sequences and annotates <i>L. bifasciata</i>'s complete mitochondrial genome (15,768 bp), which contains 13 PCGs, 22 tRNAs, 2 rRNAs. All 13 PCGs start with ATN (ATT, ATG, ATA); most end with TAA, except ND1 (TAG). Phylogenetic analysis shows <i>L. bifasciata</i> is sister to <i>Anagyrus</i> spp.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 10","pages":"919-922"},"PeriodicalIF":0.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12412320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Complete mitochondrial genome of the firefly <i>Luciola parvula</i> Kiesenwetter (Coleoptera, Lampyridae) from Japan and its phylogenetic analyses.","authors":"Yuki Selene Saito, Yuki Kaneko-Ochi, Masayuki Iigo","doi":"10.1080/23802359.2025.2554217","DOIUrl":"10.1080/23802359.2025.2554217","url":null,"abstract":"<p><p>We have sequenced the complete mitochondrial genome of the firefly <i>Luciola parvula</i> Kiesenwetter, 1874 (Coleoptera, Lampyridae, Luciolinae) from Niigata Prefecture, Honshu, Japan, and inferred the Luciolinae phylogeny. The circular genome of 16,685 bp (GenBank accession number, LC677171) has a base composition of A (43.52%), C (13.21%), G (8.52%) and T (34.74%). Our sequence is similar to other Metazoa, which contains 13 protein coding genes (PCGs), 2 rRNA genes, 22 tRNA genes and D-loop. Molecular phylogenetic analyses based on mitochondrial whole genome sequences revealed <i>L. parvula</i> as a sister group of (<i>L. unmunsana</i> [MT134039] <i>+ L. parvula</i> [OL944082]). Identity of the two <i>L. parvula</i> sequences (LC677171 and OL944082) is only 90.5% (14,064/15,537 bp), suggesting the two <i>L. parvula</i> might be different species. Further taxonomical examination including morphological analyses should be required to test if the two populations are different species.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 10","pages":"909-913"},"PeriodicalIF":0.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12406331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Complete mitochondrial genomes of biological control stains in the <i>Trichoderma harzianum</i> Rifai complex (strains DL1-3, KC1-1, and PAR10) isolated from Californian grapevines.","authors":"Christopher M Wallis, Jianchi Chen","doi":"10.1080/23802359.2025.2552822","DOIUrl":"10.1080/23802359.2025.2552822","url":null,"abstract":"<p><p>The complete mitochondrial genomes of <i>Trichoderma</i> sp. strains DL1-3 (29,197 bp), KC1-1 (27,631 bp), and PAR10 (29,122 bp), isolated from grapevine leaves, were obtained. All strains contained 25 or 26 tRNA genes, two rRNA genes, and 15 core mitochondrial protein coding genes. Phylogenetic trees made with housekeeping region and mitochondrial sequences placed these strains in the <i>T. harzianum</i> clade, with DL1-3 and PAR10 as potential novel species, and KC1-1 identified as a strain of <i>T. harzianum</i>. This suggests that complete mitochondria genome comparisons provide valuable information in resolving questions of species identification, including putatively identifying those that are novel.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 10","pages":"893-898"},"PeriodicalIF":0.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12406320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The complete chloroplast genome of <i>Coreopsis lanceolata</i> L. and its phylogenetic analysis.","authors":"Chunfeng Wan, Dexin Wang, Xiaoqin Zhang, Hongling Liang, Junwei Zhang","doi":"10.1080/23802359.2025.2555460","DOIUrl":"10.1080/23802359.2025.2555460","url":null,"abstract":"<p><p><i>Coreopsis lanceolata</i> L. 1753 is a perennial herb of the family Asteraceae, often cultivated as an ornamental flower. The species has also been reported to contain a wide range of phytochemicals and to exhibit diverse pharmacological activities. To better understand the genetic information of <i>C. lanceolata</i>, its complete chloroplast genome was assembled and characterized for the first time in this study. The chloroplast genome was 150,448 bp long and contained 129 genes, including 86 protein-coding genes, 35 tRNA genes, and eight rRNA genes. A rearrangement involving double inversions was observed in the chloroplast genome when compared with that of <i>Barnadesia caryophylla</i>. Phylogenomic analysis indicated that <i>C. lanceolata</i>, <i>Bidens frondosa</i>, <i>B. tripartita</i>, and <i>B. pilosa</i> formed a monophyletic clade. The findings of this study provide support for future research on the evolution and potential pharmaceutical applications of this species.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 10","pages":"899-903"},"PeriodicalIF":0.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12406329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhiming Dai, Fayuan Jiang, Jingpeng Zhao, Min Chen, Ruisen Lu
{"title":"The complete chloroplast genome of <i>Dioscorea tenuipes</i> Franch. & Sav. and its phylogenetic analysis.","authors":"Zhiming Dai, Fayuan Jiang, Jingpeng Zhao, Min Chen, Ruisen Lu","doi":"10.1080/23802359.2025.2556007","DOIUrl":"10.1080/23802359.2025.2556007","url":null,"abstract":"<p><p>Here, we present the first complete chloroplast genome of <i>D. tenuipes</i> (154,018 bp), which exhibits a typical quadripartite structure, including an LSC (83,966 bp), SSC (18,910 bp), and two IRs (25,571 bp each). A total of 133 genes were annotated, with 114 unique genes and 19 duplicated in the IRs. .Phylogenetic analyses based on complete chloroplast genomes of 13 <i>Stenophora</i> species strongly supported <i>D. tenuipes</i> as sister to <i>D. gracillima</i> (BS = 100%, PP = 1.0), challenging earlier studies that suggested a sister relationship with <i>D. tokoro</i> based on partial plastid sequences. Overall, this study provides a critical genomic resource for advancing research on the taxonomy, evolution, and medicinal breeding of <i>D. tenuipes</i>.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 10","pages":"904-908"},"PeriodicalIF":0.7,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12406315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The complete mitochondrial DNA sequence of the <i>Pentalagus furnessi</i> (Lagomorpha: Leporidae), an endemic species of Amami Ōshima, Japan.","authors":"Miyu Tsubota, Himeyuri Sunahata, Asahi Itoi, Mariko Suzuki, Jun-Ichi Takahashi","doi":"10.1080/23802359.2025.2552811","DOIUrl":"10.1080/23802359.2025.2552811","url":null,"abstract":"<p><p><i>Pentalagus furnessi</i>, an endemic relict rabbit restricted to Amami Ōshima and Tokunoshima Islands, Japan, is threatened by invasive carnivores, habitat loss, and roadkill. We report the first complete mitochondrial genome of <i>P. furnessi</i> obtained by next-generation sequencing, providing baseline data for conservation genetics. The mitochondrial genome (approximately 16 kb) encodes 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and a control region, with an overall A+T content of 62.8%. Start codons ATG/ATT and stop codons TAA/TAG were identified. Phylogenetic analysis using 13 PCGs revealed a distinct lineage, consistent with its monotypic genus status and supporting conservation efforts.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 10","pages":"889-893"},"PeriodicalIF":0.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jorge L Garzon, Celestino Aguilar, Kristin Saltonstall, Carlos F Arias, Catherine B Viverette, Luis F De León
{"title":"Characterization of the mitochondrial genome and phylogenetic position of the Varied Solitaire <i>Myadestes coloratus</i> (Passeriformes: Turdidae).","authors":"Jorge L Garzon, Celestino Aguilar, Kristin Saltonstall, Carlos F Arias, Catherine B Viverette, Luis F De León","doi":"10.1080/23802359.2025.2549750","DOIUrl":"10.1080/23802359.2025.2549750","url":null,"abstract":"<p><p>The Varied Solitaire (<i>Myadestes coloratus</i> Nelson, 1912) is a near-threatened bird species, endemic to the highlands of eastern Panama and the Colombian border. In this study, we report the complete mitochondrial genome of <i>M. coloratus</i>. The mitogenome exhibits a typical avian structure with a slight AT bias and a control region. Phylogenetic analysis confirms the close relationship between <i>M. coloratus</i> and other thrush (Turdidae) species, including the extinct <i>M. myadestinus</i>. This data is crucial for future research on the evolutionary history and population genetics of <i>M. Coloratus</i>. These findings are particularly relevant as the species is increasingly threatened by human expansion.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 9","pages":"878-883"},"PeriodicalIF":0.7,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wan Tang, Yushuang Chen, Chenling Zheng, Shuang Xiong, Yuan Huang
{"title":"The complete chloroplast genome of <i>Primula bella</i> (Primulaceae): a species endemic to the Hengduan Mountains in China.","authors":"Wan Tang, Yushuang Chen, Chenling Zheng, Shuang Xiong, Yuan Huang","doi":"10.1080/23802359.2025.2548838","DOIUrl":"10.1080/23802359.2025.2548838","url":null,"abstract":"<p><p>We sequenced and annotated the complete chloroplast genome of <i>Primula bella</i> (Primulaceae). The 154,775 bp genome displays a typical quadripartite structure: an 85,343 bp large single-copy (LSC) region, a 17,772 bp small single-copy (SSC) region, and two 25,830 bp inverted repeat (IR) regions. It contains 130 genes (86 protein-coding, 36 tRNA, 8 rRNA). Phylogenetic analysis revealed close relationships between <i>P. bella, Primula annulata</i>, and <i>Primula walshii</i>, supporting the monophyly of section <i>Minutissimae</i>. This study provides valuable genomic resources for <i>P. bella</i> and insights into Primulaceae phylogeny.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 9","pages":"874-877"},"PeriodicalIF":0.7,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12379693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stephan Siegert, Joel Fonseca Nogueira, Daniel Jestrzemski, Mike Reich, Bertram Brenig
{"title":"The cypriot blunt-nosed viper <i>Macrovipera lebetinus lebetinus</i>: complete mitochondrial genome revealed by next-generation sequencing.","authors":"Stephan Siegert, Joel Fonseca Nogueira, Daniel Jestrzemski, Mike Reich, Bertram Brenig","doi":"10.1080/23802359.2025.2546948","DOIUrl":"10.1080/23802359.2025.2546948","url":null,"abstract":"<p><p><i>Macrovipera lebetinus lebetinus</i> is the sole venomous snake species endemic to Cyprus, with its population declining due to habitat loss and persecution. The taxonomic status of this species remains uncertain, and there is a significant gap in available genetic information. Here, we present the first complete mitochondrial genome of <i>M. lebetinus lebetinus</i>, obtained from two specimens. The mitogenomes (17,151 bp and 17,152 bp) include the standard 37 genes-13 protein-coding genes, 22 tRNAs, and 2 rRNAs-as well as two control regions. Comparative analysis reveals a conserved gene order typical for Viperidae but also an unprecedented structural modification: the complete loss of the D-arm in tRNA-Cys, a feature previously unreported in snakes. Phylogenetic analysis confirms the monophyly of <i>M. lebetinus lebetinus</i> and <i>M. lebetinus schweizeri</i>. These findings contribute essential genetic data for this under-documented species, providing valuable molecular markers for future phylogenetic and conservation studies.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 9","pages":"852-857"},"PeriodicalIF":0.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12377087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}