Dong-Yeop Lee, Heesu Lee, Ji-Yeon Hyeon, Dong-Hun Lee
{"title":"Complete mitochondrial genome sequencing and phylogenetic analysis of the Light-vented Bulbul (<i>Pycnonotus sinensis</i>) killed by window collision in South Korea in 2023.","authors":"Dong-Yeop Lee, Heesu Lee, Ji-Yeon Hyeon, Dong-Hun Lee","doi":"10.1080/23802359.2025.2532040","DOIUrl":"10.1080/23802359.2025.2532040","url":null,"abstract":"<p><p>The Light-vented Bulbul (<i>Pycnonotus sinensis</i>) has rapidly expanded across East Asia, particularly in South Korea since the early 2000s. This study presents the complete mitochondrial genomes of five individuals died from window collisions in Seoul. The genomes, sequenced using next-generation sequencing technology, are 16,923bp long and contain 13 protein-coding genes, 22 tRNAs, and 2 rRNAs. Phylogenetic analysis revealed that South Korean <i>P. sinensis</i> forms a monophyletic clade with <i>P. sinensis hainanus</i> from China, indicating high genetic similarity. These findings provide essential data for understanding of <i>P. sinensis</i> population structure and regional adaptation.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 8","pages":"773-777"},"PeriodicalIF":0.7,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12302383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The complete mitochondrial genome of <i>Uraecha angusta</i> Pascoe, 1856 (Coleoptera: Cerambycidae) and its phylogenetic analyses.","authors":"Sikai Ke, Zibo Li, Ruiheng Lin, Yunfeng Zheng, Yinghua Tong","doi":"10.1080/23802359.2025.2532186","DOIUrl":"10.1080/23802359.2025.2532186","url":null,"abstract":"<p><p><i>Uraecha angusta</i> Pascoe, 1856 (Coleoptera: Cerambycidae) is one of the primary stem borers affecting economically significant forests. However, research on <i>U. angusta</i> remains limited. In this study, we successfully sequenced the complete mitochondrial genome of <i>U. angusta.</i> The analysis revealed that the mitochondrial genome spans 15,678 bp with a GC content of 36.7%. It comprises13 protein-coding genes (CDS), 22 transfer RNA genes (tRNA), 2 ribosomal RNA genes (rRNA), and an A + T-rich region. Phylogenetic analysis revealed that <i>U. angusta</i> is closely related to <i>Annamanum lunulatum</i>. This study offers valuable genetic insights into the evolutionary dynamics within the Cerambycidae family.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 8","pages":"768-772"},"PeriodicalIF":0.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12291189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Complete sequence of the <i>Achillea ptarmica</i> chloroplast genome determined by long-read sequencing.","authors":"Hiroki Natsume, Makoto Kusaba","doi":"10.1080/23802359.2025.2535628","DOIUrl":"10.1080/23802359.2025.2535628","url":null,"abstract":"<p><p><i>Achillea</i> species, commonly referred to as yarrow, belong to the family Asteraceae and are typically perennial, outbreeding plants used in traditional medicine. The genus <i>Achillea</i> has undergone hybridization and polyploidization events, resulting in phylogenetically complex relationships. Considering the limited scope of genomic studies on this genus, a genomic analysis of a diploid <i>Achillea</i> species is important for advancing our understanding of its evolutionary biology and genetic diversity. In this study, we assembled the complete chloroplast genome sequence of a diploid <i>Achillea</i> species (<i>A. ptarmica</i>) using nanopore long-read sequencing technology. The assembled chloroplast genome (149,252 bp), with a GC content of 38%, was revealed to include a large single-copy region (82,498 bp), a small single-copy region (18,458 bp), and two inverted repeat regions (24,148 bp each). The genome contains 89 protein-coding genes, 8 rRNAs, and 37 tRNAs. A phylogenetic analysis clustered <i>A. ptarmica</i> with other <i>Achillea</i> species (<i>A. millefolium</i> and <i>A. wilsoniana</i>), positioning them within the same clade as the genus <i>Tanacetum</i>. The accuracy of nanopore sequencing was validated by comparing the results for 10 chloroplast genes with the corresponding Sanger sequencing results. Our findings provide valuable genetic resources for further taxonomic, evolutionary, and phylogenetic studies of the genus <i>Achillea</i>.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 8","pages":"753-757"},"PeriodicalIF":0.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12288161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yin-Tao Zhang, Cheng-Pu Lu, Yu-Hui Tao, Cheng-Wei Tong, Jie Chen, Wei Liu
{"title":"The complete mitochondrial genome of <i>Rhynchocypris czekanowskii</i> (Cypriniformes, Cyprinidae).","authors":"Yin-Tao Zhang, Cheng-Pu Lu, Yu-Hui Tao, Cheng-Wei Tong, Jie Chen, Wei Liu","doi":"10.1080/23802359.2025.2535639","DOIUrl":"10.1080/23802359.2025.2535639","url":null,"abstract":"<p><p>Here we present the first complete mitochondrial genome assembly of <i>Rhynchocypris czekanowskii</i> Dybowski, 1869. We characterized a 16,651-bp circular genome comprising 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a 981-bp control region. Phylogenetic reconstruction using mitochondrial sequences robustly resolved <i>R. czekanowskii</i> as sister to <i>R. lagowskii</i> (bootstrap = 100%), supporting morphological affinities. This work fills a critical genomic gap in <i>Rhynchocypris</i> phylogenetics and has significant implications for both taxonomic revision and the conservation of cold-adapted ichthyofauna in Northeast Asia.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 8","pages":"763-767"},"PeriodicalIF":0.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12288181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zheng-Kai Ye, Guo-Yong Yang, Ye Tang, Qin-Yu Liu, Li-Hua Chen, Xiang-Hua Fang
{"title":"Complete chloroplast genome analysis of <i>Hypopterygium flavolimbatum</i> Müll. Hal. (Hypopterygiaceae, Bryophyta).","authors":"Zheng-Kai Ye, Guo-Yong Yang, Ye Tang, Qin-Yu Liu, Li-Hua Chen, Xiang-Hua Fang","doi":"10.1080/23802359.2025.2535629","DOIUrl":"10.1080/23802359.2025.2535629","url":null,"abstract":"<p><p>To address limited genomic resources hindering <i>Hypopterygium</i> phylogenetics/taxonomy, the chloroplast genome of <i>Hypopterygium flavolimbatum</i> Müll. Hal. (Hypopterygiaceae, Bryophyta) was sequenced. The chloroplast genome is 125,283 bp in length, comprising an 86,845 bp large single-copy region, an 18,606 bp small single-copy region, and a pair of 9916 bp inverted repeats. A total of 127 genes were predicted, including 82 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. Phylogenetic analysis indicates that <i>H. flavolimbatum</i> exhibits the closest genetic affinity with a clade containing <i>Myurella julacea</i>, <i>Myuroclada maximowiczii</i>, and <i>Climacium dendroides</i>. This study provides essential sequence data for species identification and phylogenetics.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 8","pages":"758-762"},"PeriodicalIF":0.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12284983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of the complete mitochondrial genome of a rare fungal species <i>Mutinus fleischeri</i> Penz.","authors":"Jing Song, Qiang Gao, Fang-Neng Lou, Peng-Xiang Wang, Chun-Guang Zhang, Hong-Yin Tang, Zhan-Ping Huang","doi":"10.1080/23802359.2025.2528576","DOIUrl":"10.1080/23802359.2025.2528576","url":null,"abstract":"<p><p>The complete mitochondrial genome of <i>Mutinus fleischeri</i> Penz 1899, a rare fungal species of Phallales, was sequenced, assembled and annotated in this study. The mitochondrial genome of <i>M. fleischeri</i> was assembled as a circular DNA molecule with a length of 113,076 base pairs (bp). It encodes 47 protein-coding genes and 24 transfer RNA (tRNA) genes. The nucleotide composition of the <i>M. fleischeri</i> mitochondrial genome is as follows: adenine (A: 38.59%), thymine (T: 36.00%), guanine (G: 13.75%), and cytosine (C: 11.66%). This in-depth phylogenetic analysis based on mitochondrial genes suggests that <i>M. fleischeri</i> shares a close evolutionary relationship with both <i>Phallus indusiatus</i> Vent 1798 and <i>Phallus echinovolvatus</i> (M. Zang, D.R. Zheng, and Z.X. Hu) Kreisel 1996.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 8","pages":"748-752"},"PeriodicalIF":0.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aijing Meng, Hong Wang, Liufanzhi Ye, Yan Zhi, Chuntian Zhang, Jiayu Liu
{"title":"The complete mitochondrial genome of <i>Aneogmena fischeri</i> (Brauer & Bergenstamm, 1891) (Diptera: Tachinidae) with phylogenetic analysis.","authors":"Aijing Meng, Hong Wang, Liufanzhi Ye, Yan Zhi, Chuntian Zhang, Jiayu Liu","doi":"10.1080/23802359.2025.2528573","DOIUrl":"10.1080/23802359.2025.2528573","url":null,"abstract":"<p><p>The complete mitochondrial genome of <i>Aneogmena fischeri</i> was sequenced and assembled. The circular genome spans 15,411 bp and contains thirteen protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and one control region (GenBank accession no. OR537869). The nucleotide composition shows a significant AT bias: 41.01% adenine (A), 39.94% thymine (T), 7.96% guanine (G), and 11.10% cytosine (C). The phylogenetic analysis of 76 species in the family Tachinidae was performed. Phylogenetic results revealed that <i>A. fischeri</i> forms sister relationship with genus <i>Blepharipa</i> Rondani. Tribe Goniini was identified as the sister group to the majority of tribe Eryciini.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 8","pages":"709-713"},"PeriodicalIF":0.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12278469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Feiming Chen, Hao Wu, Min Xie, Zhenzhen Xiong, Jia Cai, Yu Huang, Xiao Jin, Can Yang, Jinwei Gao, Rui Song
{"title":"Characterizing the complete mitogenome of <i>Heterosentis holospinus</i> Amin, Heckman & Ha, 2011 (Palaeacanthocephala: Echinorhynchida: Arhythmacanthidae) and its mitochondrial phylogeny.","authors":"Feiming Chen, Hao Wu, Min Xie, Zhenzhen Xiong, Jia Cai, Yu Huang, Xiao Jin, Can Yang, Jinwei Gao, Rui Song","doi":"10.1080/23802359.2025.2528571","DOIUrl":"10.1080/23802359.2025.2528571","url":null,"abstract":"<p><p><i>Heterosentis holospinus</i> Amin, Heckman & Ha, 2011 is a parasitic acanthocephalan, with <i>Cynoglossus bilineatus</i> Lacepède, 1802 identified as a new definitive host. We sequenced and annotated its complete mitochondrial genome, which spans 16,560 bp and contains 36 genes, including 22 tRNAs, 12 PCGs and 2 rRNAs. Phylogenetic analysis supports the monophyly of <i>Heterosentis</i> and its current taxonomic status. This study enriches essential molecular data that contribute to a clearer phylogenetic framework for Acanthocephala and highlight the utility of mitochondrial genomes in resolving taxonomic relationships at both the genus and family levels.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 8","pages":"742-747"},"PeriodicalIF":0.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12272951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Changmei Du, Tingting Xiong, Yan Dong, Haishuo Gao, Yan Zhang, Jianhua Yue
{"title":"Complete chloroplast genome structural and phylogenetic analysis of <i>Physostegia virginiana</i> (L.) Benth. 1930 (Lamiaceae).","authors":"Changmei Du, Tingting Xiong, Yan Dong, Haishuo Gao, Yan Zhang, Jianhua Yue","doi":"10.1080/23802359.2025.2528568","DOIUrl":"10.1080/23802359.2025.2528568","url":null,"abstract":"<p><p><i>Physostegia virginiana</i> (L.) Benth. (1930) is an ornamental plant with great potential for landscaping application and cut-flower using in China. To explore its phylogenetic position and analyze the characteristics of its chloroplast (cp) genome, this study sequenced the whole cp genome of <i>P. virginiana</i>. The results revealed that the total length of the cp genome was 152,880 bp, and the lengths of the large single-copy region (LSC) and small single-copy region (SSC) were 84,428 and 17,396 bp, respectively. The lengths of the inverted repeat regions (IRa and IRb) were both 25,528 bp. The cp genome of <i>P. virginiana</i> contains 131 coding genes, namely, 86 protein-coding genes, 37 tRNA genes and eight rRNA genes. The total GC content of the cp genome was 38.38%. The phylogenetic results revealed that <i>P. virginiana</i> was closely related to <i>Gomphostemma lucidum</i>. The results of this study provide a molecular basis for the phylogenetic localization of <i>P. virginiana</i> and basic data for its identification and resource development.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 8","pages":"736-741"},"PeriodicalIF":0.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The complete chloroplast genome of <i>Mitella nuda</i> (Saxifragaceae).","authors":"Yangling Peng, Tiange Yang, Hong Liu","doi":"10.1080/23802359.2025.2529830","DOIUrl":"10.1080/23802359.2025.2529830","url":null,"abstract":"<p><p>The genus <i>Mitella</i> L. 1753 belongs to the family Saxifragaceae. Studying the chloroplast genome of <i>Mitella</i> is of great significance for understanding the phylogeny within the <i>Heuchera</i> group. This study reports the chloroplast genome of <i>Mitella nuda</i>, which has a total length of 155,409 bp. The chloroplast genome contains 131 genes. The phylogenetic tree indicates that <i>Mitella</i> is not monophyletic and exhibits clear phylogenetic relationships with <i>Heuchera</i> and <i>Tiarella</i>. This study not only enriches the genomic data for <i>Mitella</i> but also provides valuable data support for evolutionary studies within the Saxifragaceae family.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. Part B, Resources","volume":"10 8","pages":"719-724"},"PeriodicalIF":0.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}