Methods and Applications in Fluorescence最新文献_第6页

Excited-state properties of 6-methoxyflavone in the presence of halide ions in aqueous media. 卤化物离子存在下6-甲氧基黄酮的激发态性质。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-07-07 DOI: 10.1088/2050-6120/ace152

Nisha Fatma, Sanjay Pant, Nupur Pandey, Mohan Singh Mehata

{"title":"Excited-state properties of 6-methoxyflavone in the presence of halide ions in aqueous media.","authors":"Nisha Fatma, Sanjay Pant, Nupur Pandey, Mohan Singh Mehata","doi":"10.1088/2050-6120/ace152","DOIUrl":"https://doi.org/10.1088/2050-6120/ace152","url":null,"abstract":"The present work investigated the influence of different halides on the excited state dynamics of 6-methoxyflavone (6MF) in an aqueous solution with steady-state and time-resolved techniques. On successive addition of I-and Br-ions, the fluorescence of 6MF quenched significantly, whereas the respective ions do not change the maximum fluorescence band. Fluorescence of 6MF was quenched 66% by I-ions and 34% by Br-ions. In a pure aqueous medium, both the H-bonded: CT and protonated species of 6MF participate in the quenching of fluorescence. The quenching process was categorized by Stern-Volmer (S-V) and Lehrer equations. Quenching parameters such as KSV, KSV-Land kqwere higher for I-ions than Br-ions. The decrease in fluorescence intensity and a reduction in fluorescence lifetime suggested the dynamic nature of quenching by I-ions following the electron transfer mechanism. Fluorescence quenching of 6MF has also been observed in the acidic medium in the presence of different halides. Thus, the study reveals that 6MF is responsive towards I-ions in a wide range of pH, specifically in a purely aqueous environment (pH∼7), hence important for sensing/detection applications.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10157473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time correction of chromatic aberration in optical fluorescence microscopy. 光学荧光显微镜色差的实时校正。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-07-03 DOI: 10.1088/2050-6120/ace153

Ana Cayuela López, Pablo Conesa, Ana María Oña Blanco, José Antonio Gómez-Pedrero, Carlos Oscar S Sorzano

{"title":"Real-time correction of chromatic aberration in optical fluorescence microscopy.","authors":"Ana Cayuela López, Pablo Conesa, Ana María Oña Blanco, José Antonio Gómez-Pedrero, Carlos Oscar S Sorzano","doi":"10.1088/2050-6120/ace153","DOIUrl":"https://doi.org/10.1088/2050-6120/ace153","url":null,"abstract":"Multi-color fluorescence imaging is a powerful tool for studying the spatial relationships and interactions among sub-cellular structures in biological specimens. However, if improperly corrected, geometrical distortions caused by mechanical drift, refractive index mismatch, or chromatic aberration can lead to lower image resolution. In this paper, we present an extension of the image processing framework of Scipion by integrating a protocol called OFM Corrector, which corrects geometrical distortions in real-time using a B-spline-based elastic continuous registration technique. Our proposal provides a simple strategy to overcome chromatic aberration by digitally re-aligning color channels in multi-color fluorescence microscopy images, even in 3D or time. Our method relies on a geometrical calibration, which we do with fluorescent beads excited by different wavelengths of light and subsequently registered to get the elastic warp as a reference to correct chromatic shift. Our software is freely available with a user-friendly GUI and can be broadly used for various biological imaging problems. The paper presents a valuable tool for researchers working in light microscopy facilities.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9734822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Two-photon lifetime-based photoconversion of EGFP for 3D-photostimulation in FLIM. 基于双光子寿命的EGFP在FLIM中用于3d光刺激的光转换。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-06-12 DOI: 10.1088/2050-6120/acdb31

Dita Strachotová, Aleš Holoubek, Barbora Brodská, Petr Herman

引用次数: 0

Study of the senescence of rice leaves through stationary and time-resolved photoluminescence. 用固定光致发光和时间分辨光致发光研究水稻叶片衰老。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-06-12 DOI: 10.1088/2050-6120/acda7b

Gerardo Fonthal, Liliana Tirado-Mejía, Luz Angela Giraldo-Pinto

{"title":"Study of the senescence of rice leaves through stationary and time-resolved photoluminescence.","authors":"Gerardo Fonthal, Liliana Tirado-Mejía, Luz Angela Giraldo-Pinto","doi":"10.1088/2050-6120/acda7b","DOIUrl":"https://doi.org/10.1088/2050-6120/acda7b","url":null,"abstract":"This work describes the relationship between the complex of photosystem I and photosystem II in the senescence process of rice leaves observed through changes in the optical response. We studied three varieties of rice plants at different aging times using time-resolved photoluminescence to measure the time decay of the emission, and stationary photoluminescence, to measure the emission wavelength. The spectra obtained with the former technique were fitted with decreasing exponential functions. Two relaxation times were obtained, one ranging between 1.0 and 1.7 ns, and the other, from 5.0 to 10.5 ns. They are associated with the electron's deexcitation of PSI and PSII, respectively, and these decay times increase as the leaf senescence process takes place. The spectra obtained with stationary photoluminescence were fitted with Voigt functions. These spectra exhibit two main peaks around 683 and 730 nm, which could be associated mainly with PSII and PSI emissions, respectively. The PSI de-excitation exhibits higher dispersive processes because chlorophyll-a molecules in it move away from each other, decreasing their concentration. Therefore, it takes longer for electrons to recombine during photosynthesis, as seen in the time-resolve response. Articulating the results of both photoluminescence techniques, the changes in the response of the photosystems of the living rice leaves during senescence are evidenced.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9616049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of polyvinylpyrrolidone (PVP) in the synthesis of luminescent NaYF₄:Yb,Er upconversion nanoparticles. 聚乙烯吡咯烷酮(PVP)对合成发光NaYF4:Yb,Er上转化纳米粒子的影响。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-05-30 DOI: 10.1088/2050-6120/acd837

Ross Birch, Jochen Bruckbauer, Marta Gajewska, Grzegorz Cios, Robert Pal, Lewis E MacKenzie

{"title":"Influence of polyvinylpyrrolidone (PVP) in the synthesis of luminescent NaYF4:Yb,Er upconversion nanoparticles.","authors":"Ross Birch, Jochen Bruckbauer, Marta Gajewska, Grzegorz Cios, Robert Pal, Lewis E MacKenzie","doi":"10.1088/2050-6120/acd837","DOIUrl":"https://doi.org/10.1088/2050-6120/acd837","url":null,"abstract":"Polyvinylpyrrolidone (PVP) can be used to produce upconversion nanoparticles (UCNPs) in an advantageous manner, i.e. at modest temperatures in open-to-air conditions with simple hotplate and flask apparatus. However, the influence of PVP parameters on the formation of UCNPs has not been previously investigated. In this exploratory study, we establish that PVP molecular weight and relative amount of PVP can greatly influence the morphology and diameter of NaYF4:Yb,Er UCNPs produced via the PVP-assisted route. At nominal amounts of PVP, varying the molecular weight of PVP in synthesis between 10,000 g mol-1(PVP10), 40,000 g mol-1(PVP40), and 55,000 g mol-1(PVP55), had minimal effect on UCNP morphology, whereas reducing the quantity of PVP10 and PVP40 in the reaction to 10% of the nominal amount resulted in two notable effects: (1) the generation of a greater range of UCNP diameters and (2) the production of an unexpected sub-population of rhombus-shaped UCNPs. Bulk and individual nanoparticle analysis indicates that all UCNP morphologies were cubic (α-phase) crystal structure and consisted of NaYF4:Yb,Er. Optical emission properties exhibited only modest green and red luminescence emission ratio when PVP parameters were varied. However, separately produced PVP40 NaYF4:Yb,Tm UCNPs exhibited a much more intense and dual-band blue/red emission. This exploratory work demonstrates that tailoring PVP content in synthesis of UCNPs can greatly alter morphology of UCNPs produced and should be carefully considered in experimental design. However, the underlying mechanisms of action of the role PVP plays in this synthesis remain unclear. Ultimately, significant further work is still required to fully elucidate the relevant chemistry to achieve full control of PVP-UCNP synthesis.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9537912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative study of conventional FRET and light harvesting properties of Rh-110/Rh-6G and Rh-19/Rh-B organic dye pairs impregnated in sol-gel glasses. 在溶胶-凝胶玻璃中浸渍的Rh-110/Rh-6G和Rh-19/Rh-B有机染料对的常规FRET和光捕获性能的比较研究。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-05-05 DOI: 10.1088/2050-6120/accfe4

Kapil Dev Mahato, Uday Kumar

{"title":"A comparative study of conventional FRET and light harvesting properties of Rh-110/Rh-6G and Rh-19/Rh-B organic dye pairs impregnated in sol-gel glasses.","authors":"Kapil Dev Mahato, Uday Kumar","doi":"10.1088/2050-6120/accfe4","DOIUrl":"https://doi.org/10.1088/2050-6120/accfe4","url":null,"abstract":"Two different pairs of laser dyes, Rhodamine-110 (Rh-110)/Rhodamine-6G (Rh-6G) and Rhodamine-19 (Rh-19)/Rhodamine-B (Rh-B) (the first dye in each pair as a donor and the second as an acceptor) were impregnated in silica samples prepared by the sol-gel method and spectroscopically studied using absorption and steady-state fluorescence techniques. The critical transfer distance (R0), actual distance (r) between the donor and acceptor, overlap integral [J(υ¯)], FRET (fluorescence resonance energy transfer) efficiency (E), and antenna effect efficiency (AE) were investigated in detail based on the variation in acceptor concentration. The FRET efficiency, antenna effect efficiency, and actual donor-acceptor distance for Rh-110/Rh-6G and Rh-19/Rh-B dye pairs corresponding to acceptor concentration ranges (3.83 to 7.65) × 10-5M l-1and (3.71 to 8.34) × 10-5M l-1, respectively, were found to be in the ranges of 57.38% to 74.89%, 36.97% to 24.13%, 5.44 nm to 4.77 nm, and 77.01%. Furthermore, maximum FRET efficiencies of 85.68% and 87.63% and antenna effect efficiencies of 36.97% and 40.95% for Rh-110/Rh-6G and Rh-19/Rh-B, respectively, were also reported. Our results demonstrate the superior FRET efficiency of Rh-19/Rh-B over Rh-110/Rh-6G dye pair in sol-gel glasses, while the antenna effect efficiency of Rh-110/Rh-6G is higher than that of Rh-19/Rh-B for the same donor to acceptor (D/A) ratio. Finally, Rh-110/Rh-6G is a better energy harvester than the Rh-19/Rh-B dye pair at the common D/A ratio. These results are explained in terms of molecular structure similarity, polarity, and rigidity of donor and acceptor.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9766784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual emission and itsλ-ratiometric detection in analytical fluorimetry. Pt. I. Basic mechanisms of generating the reporter signal. 分析荧光法中的双发射及其λ-比率检测。第1章报告信号产生的基本机制。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-04-21 DOI: 10.1088/2050-6120/acc714

Alexander Demchenko

引用次数: 1

Surface plasmon enhanced fluorescence: self-consistent classical treatment in the quasi-static limit. 表面等离子体增强荧光:准静态极限下的自洽经典处理。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-04-20 DOI: 10.1088/2050-6120/acca62

Dentcho A Genov

{"title":"Surface plasmon enhanced fluorescence: self-consistent classical treatment in the quasi-static limit.","authors":"Dentcho A Genov","doi":"10.1088/2050-6120/acca62","DOIUrl":"https://doi.org/10.1088/2050-6120/acca62","url":null,"abstract":"The problem of enhanced molecular emission in close proximity to dielectric and metallic interfaces is of great importance for many physical and biological applications. Here we present an exact treatment of the problem from the view point of classical electromagnetism. Self-consistent analytical theory of the surface enhanced fluorescence (SEF) is developed for configurations consisting of an emitter in proximity to core-shell metal-dielectric nanoparticles. The dependence of the fluorescence enhancement on the excitation laser and fluorescence frequencies and distance of the emitter to the nanoparticle interface are studied. The developed theory predicts enhanced fluorescence at intermediate distances as well as emission quenching into non-radiative surface plasmon (SP) modes dominating the response for short distances. The conditions for optimal emission enhancement for two core-shell configurations are determined and a comparison to published experimental data is performed showing a good correspondence between theory and experiment. The developed model can be applied toward analyzes and optimizations of various applications related to SP enhance fluorescence spectroscopy.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9325749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual emission and itsλ-ratiometric detection in analytical fluorimetry Pt. II. Exploration in sensing and imaging. 分析荧光法中的双发射及其λ-比率检测[j]。传感与成像探索。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-04-18 DOI: 10.1088/2050-6120/acc715

Alexander P Demchenko

{"title":"Dual emission and itsλ-ratiometric detection in analytical fluorimetry Pt. II. Exploration in sensing and imaging.","authors":"Alexander P Demchenko","doi":"10.1088/2050-6120/acc715","DOIUrl":"https://doi.org/10.1088/2050-6120/acc715","url":null,"abstract":"The wavelength-ratiometric techniques demonstrate strong advantages in fluorescence sensing and imaging over techniques employing variations of intensity at single wavelength. We present different possibilities for realization of these advantages in different simplified, miniaturized and multiplexing devices. They include the smartphone-based detection systems and strips, in which the color changes are observed with naked eye. The array-based techniques and different immunoassays withλ-ratiometric detection demonstrate strongly increased stability and sensitivity. The application areas extend from on-site monitoring of environment and point-of-care diagnostics to testing in personal need. Selected examples of sensing different analytes in chemical and biological systems demonstrate multiple possibilities of coupling the analyte-sensor interaction with the generation ofλ-ratiometric output signal. Among them, simultaneous detection of several analytes and performing logical operations that can be useful in analysis. Finally, the benefits of multicolor ratiometric fluorescence imaging are demonstrated by visualization the functionally important parameters of biological membranes.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9377952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Corrigendum: Pulse-shaped broadband multiphoton excitation for single-molecule fluorescence detection in the far field (2023Methods Appl. Fluoresc.11017001). 勘误:用于远场单分子荧光检测的脉冲形宽带多光子激发(2023)Fluoresc.11017001)。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-04-14 DOI: 10.1088/2050-6120/acca63

David Nobis, Henry G Sansom, Steven W Magennis

引用次数: 0