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Methods and Applications in Fluorescence最新文献

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Spectrofluorometric determination of Fe3+, Pd2+, and Sn2+ions using carbon nanodots derived from hazelnut shells. 用榛子壳碳纳米点分光光度法测定Fe3+、Pd2+和Sn2+离子。
IF 2.4 3区 化学
Methods and Applications in Fluorescence Pub Date : 2025-05-12 DOI: 10.1088/2050-6120/add1aa
Abidin Gümrükçüoğlu, Miraç Ocak, Sevgi Kolaylı, Saliha Dinç, Ilknur Altin, Serhat Gün, Ümmühan Turgut Ocak
{"title":"Spectrofluorometric determination of Fe<sup>3+</sup>, Pd<sup>2+</sup>, and Sn<sup>2+</sup>ions using carbon nanodots derived from hazelnut shells.","authors":"Abidin Gümrükçüoğlu, Miraç Ocak, Sevgi Kolaylı, Saliha Dinç, Ilknur Altin, Serhat Gün, Ümmühan Turgut Ocak","doi":"10.1088/2050-6120/add1aa","DOIUrl":"https://doi.org/10.1088/2050-6120/add1aa","url":null,"abstract":"<p><p>Carbon nanodots (CNDs) were synthesized from hazelnut shells using the pyrolysis method in an oven at temperatures ranging from 250 to 400 °C and reaction times between 1 and 3 h. The structural and optical properties of the CNDs, which exhibited strong blue fluorescence under a UV lamp (365 nm), were characterized using UV-vis absorption spectroscopy, fluorescence spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, x-ray diffraction (XRD), x-ray photoelectron spectroscopy (XPS), and transmission electron microscopy (TEM). The quantum yield of the CNDs was determined to be between 2.2% and 7.8%. The effect of various cations on the fluorescence spectra of the CNDs was investigated using fluorescence spectrometry. Among the synthesized CNDs, those prepared via pyrolysis for 3 h at 300 °C, 315 °C, and 350 °C (designated as HS300-3, HS315-3, and HS350-3, respectively) exhibited selective fluorescence quenching in the presence of Fe<sup>3+</sup>, Sn<sup>2+</sup>, and Pd<sup>2+</sup>. These CNDs were applied for the determination of Fe<sup>3+</sup>in tap water and soil samples, while HS315-3 was also employed for the detection of Sn<sup>2+</sup>and Pd<sup>2+</sup>in tap water. The limits of detection (LOD) were 4.5 μg l<sup>-1</sup>for Fe<sup>3+</sup>and 15.8 μg l<sup>-1</sup>for both Sn<sup>2+</sup>and Pd<sup>2+</sup>. The accuracy of the methods was validated through spiked recovery experiments with tap water samples and by analyzing a certified reference material (CRM-SA-C Sandy Soil C).</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"13 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Selective detection of Cr(III) among various chromium oxidation states in aqueous environments. 水环境中不同氧化态Cr（III）的选择性检测。
IF 2.4 3区 化学
Methods and Applications in Fluorescence Pub Date : 2025-05-07 DOI: 10.1088/2050-6120/add080
Mengyu Yin, Long Yu, Kaili Wu, Yifei Sun, Yunfei Lu, Zhaojing Yuan, Wenyuan Jiang, Xiangxue Wang, Suhua Wang
{"title":"Selective detection of Cr(III) among various chromium oxidation states in aqueous environments.","authors":"Mengyu Yin, Long Yu, Kaili Wu, Yifei Sun, Yunfei Lu, Zhaojing Yuan, Wenyuan Jiang, Xiangxue Wang, Suhua Wang","doi":"10.1088/2050-6120/add080","DOIUrl":"https://doi.org/10.1088/2050-6120/add080","url":null,"abstract":"<p><p>Chromium (III), as a significant environmental pollutant, poses a serious threat to human health when accumulated in excess, making it imperative to develop highly sensitive and rapid on-site detection methods. In this study, a Eu<sup>3+</sup>-based metal-organic framework (MOF) was successfully synthesized using a dual-ligand strategy, enabling ratiometric fluorescence detection of Cr(III). During the detection process, distinct changes in the fluorescence ratio were observed, accompanied by a notable color shift from red to blue, providing a clear visual cue. Aromatic π-conjugated organic ligands were selected to enhance the photoluminescence properties, while a second ligand was introduced to optimize the crystal size and pore structure of the MOF. The experimental results demonstrated that the MOF material exhibited outstanding ratiometric fluorescence properties for Cr(III) detection, with high stability. Additionally, the material showed excellent selectivity, anti-interference ability, and sensitivity for detecting Cr(III) in water environments, with a detection range of 0-24 μM and detection limit as low as 59 nM. Further investigation revealed that the changes in ratiometric fluorescence signals were induced by Cr(III)-specific partial collapse of the crystal structure and ligand release. Finally, a MOF-based test strip was developed, where the vivid fluorescence color changes enabled visual detection by the naked eye. The test strip also demonstrated good recovery efficiency in real water samples, further confirming the material's potential as a real-time smart sensor for Cr(III) detection.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"13 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Plasmon-enhanced fluorescence of polyvinylpyrrolidone-embedded CdSeS/ZnS quantum dots on gold film over nanospheres. 纳米球金膜上聚乙烯吡咯烷酮包埋CdSeS/ZnS量子点的等离子体增强荧光。
IF 2.4 3区 化学
Methods and Applications in Fluorescence Pub Date : 2025-05-02 DOI: 10.1088/2050-6120/add3c0
Alexandra Falamas, C Farcau
{"title":"Plasmon-enhanced fluorescence of polyvinylpyrrolidone-embedded CdSeS/ZnS quantum dots on gold film over nanospheres.","authors":"Alexandra Falamas, C Farcau","doi":"10.1088/2050-6120/add3c0","DOIUrl":"https://doi.org/10.1088/2050-6120/add3c0","url":null,"abstract":"<p><p>This study explores the fluorescence enhancement of quantum dots (QDs) by gold film over nanospheres (AuFoN) plasmonic substrates, focusing on how a polymer matrix, surface functionalization, and plasmon resonances of the substrate affect the fluorescence properties of QDs. It was observed that a polyvinylpyrrolidone (PVP) matrix facilitated the uniform distribution of QDs on the surface of the AuFoN, avoiding aggregation during solvent evaporation. Progressive fluorescence redshifts and fluorescence intensity enhancement were observed when moving from QDs on glass substrates to planar Au, and most pronouncedly, to nanostructured AuFoN substrates. Among different AuFoN substrates, those based on 600 - 700 nm spheres provided the strongest fluorescence amplification due to stronger localized electromagnetic fields. Time-resolved fluorescence measurements revealed two primary fluorescence lifetime components for QDs on AuFoN films: a short component linked to non-radiative plasmonic energy transfer and a long component representing intrinsic QDs emission. By optimizing sphere size, Au nanostructured films can be tailored to control QDs fluorescence lifetimes and intensity, advancing their use in biosensing, photonics, and other fluorescence-based technologies. This work enhances our understanding of how substrate design and matrix effects impact QDs fluorescence, providing a pathway for precisely engineered SEF platforms suited to various applications in optics and photonics.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantum dots - polymer films as the reversible luminescent wide range temperature sensors. 量子点-聚合物薄膜作为可逆发光宽范围温度传感器。
IF 2.4 3区 化学
Methods and Applications in Fluorescence Pub Date : 2025-03-06 DOI: 10.1088/2050-6120/adb9b6
Dmitriy O Sagdeev, Yuriy G Galyametdinov
{"title":"Quantum dots - polymer films as the reversible luminescent wide range temperature sensors.","authors":"Dmitriy O Sagdeev, Yuriy G Galyametdinov","doi":"10.1088/2050-6120/adb9b6","DOIUrl":"10.1088/2050-6120/adb9b6","url":null,"abstract":"<p><p>The paper considers the temperature sensitivity of luminescence of three polymer types films: polymethyl methacrylate, polystyrene and polylactide doped with core-shell CdSe/CdS/ZnS and Cd<sub>0.9</sub>Mn<sub>0.1</sub>S/ZnS quantum dots. Films with uniform distribution of quantum dots in the polymer matrix were obtained by the spin-coating method. The influence of the quantum dots and polymer type, as well as their mutual content in the composite, on the thermal sensitivity and thermal stability of the films is considered. The thermal stability of the obtained composites was studied during multiple heating-cooling cycles to 100 and 175 °C, and the conditions for obtaining reusable reversible luminescent thermal sensors were established.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microwave synthesis of molybdenum disulfide quantum dots and the application in bilirubin sensing. 微波合成二硫化钼量子点及其在胆红素传感中的应用。
IF 2.4 3区 化学
Methods and Applications in Fluorescence Pub Date : 2025-01-30 DOI: 10.1088/2050-6120/ada99b
Shu Pang, Xinyu Chang, Lin Xu, Jiazhen Wu
{"title":"Microwave synthesis of molybdenum disulfide quantum dots and the application in bilirubin sensing.","authors":"Shu Pang, Xinyu Chang, Lin Xu, Jiazhen Wu","doi":"10.1088/2050-6120/ada99b","DOIUrl":"10.1088/2050-6120/ada99b","url":null,"abstract":"<p><p>Molybdenum disulfide quantum dots (MoS<sub>2</sub>QDs) is a new type of graphite like nanomaterial, which exhibited well chemical stability, unique fluorescence characteristics, and excellent biocompatibility. The conventional hydrothermal synthesis of MoS<sub>2</sub>generally requires a long-term reaction at high temperature and high pressure. Herein, we have developed a simple and fast MoS<sub>2</sub>QDs synthesis scheme using microwave heating, and further modified the surface of MoS<sub>2</sub>QDs using 3-aminophenylboronic acid. The 3- aminophenylboronic acid modified MoS<sub>2</sub>QDs (B-MoS<sub>2</sub>QDs) were further coated by a zinc-based metal-organic backbone (ZIF-8) in a solution containing zinc ions and 2-methylimidazolium. The constructed nanohybrid B-MoS<sub>2</sub>@ZIF-8 were successfully applied to the visualization and rapid detection of bilirubin based on the ratiometric fluorescence changes. The linear range for bilirubin detection is 0.2-75 μmol·l<sup>-1</sup>, and detection limit is 0.017 μmol·l<sup>-1</sup>.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cell viability assessment by using GelRed/SYTO 9-based double staining. 采用GelRed/SYTO - 9双染色法评估细胞活力。
IF 2.4 3区 化学
Methods and Applications in Fluorescence Pub Date : 2025-01-22 DOI: 10.1088/2050-6120/adaa4e
Yueping Zheng, Jian Sun, Xiaocui Li, Honxin Qiu, Lifang Liao, Shengbin He
{"title":"Cell viability assessment by using GelRed/SYTO 9-based double staining.","authors":"Yueping Zheng, Jian Sun, Xiaocui Li, Honxin Qiu, Lifang Liao, Shengbin He","doi":"10.1088/2050-6120/adaa4e","DOIUrl":"10.1088/2050-6120/adaa4e","url":null,"abstract":"<p><p>Cell viability assessment plays a crucial role in biological research, pharmaceutical development, and toxicological identification. Here, we used GelRed, a sensitive and safer nucleic acid dye, to selectively label dead cells with red fluorescence (FL) thus distinguishing dead cells from live ones. Further more, the combined use of GelRed and SYTO 9 (another nucleic acid dye) enabled the clear differentiation in FL spectra between the two physiological statuses. The GelRed and SYTO 9 concentrations were optimized to obtain the highest FL ratio of dead to live cells. The GelRed/SYTO 9-based double staining could quantify the cell viability through flow cytometry analysis, with a good correlation between the detected and theoretical dead cell ratios. Compared with traditional prodium iodide (PI) staining, the GelRed/SYTO 9-based double staining showed high accuracy in quantifying dead cell of low levels. The as-developed staining method could be used in biomedical research to accurately measure the cytotoxic effect of various substances in living cells.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microwave synthesis of molybdenum disulfide quantum dots and the application in bilirubin sensing. 微波合成二硫化钼量子点及其在胆红素传感中的应用。
IF 2.4 3区 化学
Methods and Applications in Fluorescence Pub Date : 2025-01-13 DOI: 10.1088/2050-6120/ada9b9
Shu Pang, Xinyu Chang, Lin Xu, Jiazhen Wu
{"title":"Microwave synthesis of molybdenum disulfide quantum dots and the application in bilirubin sensing.","authors":"Shu Pang, Xinyu Chang, Lin Xu, Jiazhen Wu","doi":"10.1088/2050-6120/ada9b9","DOIUrl":"https://doi.org/10.1088/2050-6120/ada9b9","url":null,"abstract":"<p><p>Molybdenum disulfide quantum dots (MoS2 QDs) is a new type of graphite like nanomaterial, which exhibited well chemical stability, unique fluorescence characteristics, and excellent biocompatibility. The conventional hydrothermal synthesis of MoS2 generally requires a long-term reaction at high temperature and high pressure. Herein, we have developed a simple and fast MoS2 QDs synthesis scheme using microwave heating, and further modified the surface of MoS2 QDs using 3-aminophenylboronic acid. The 3- aminophenylboronic acid modified MoS2 QDs (B-MoS2 QDs) were further coated by a zinc-based metal-organic backbone (ZIF-8) in a solution containing zinc ions and 2-methylimidazolium. The constructed nanohybrid B-MoS2@ZIF-8 were successfully applied to the visualization and rapid detection of bilirubin based on the ratiometric fluorescence changes. The linear range for bilirubin detection is 0.2-75 μmol•L-1, and detection limit is 0.017 μmol•L-1.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142979190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial: MAF journal and conference build together. MAF期刊和会议共同构建。
IF 2.4 3区 化学
Methods and Applications in Fluorescence Pub Date : 2024-12-17 DOI: 10.1088/2050-6120/ad9a9e
David J S Birch, Marcia Levitus, Yves Mély
{"title":"Editorial: MAF journal and conference build together.","authors":"David J S Birch, Marcia Levitus, Yves Mély","doi":"10.1088/2050-6120/ad9a9e","DOIUrl":"10.1088/2050-6120/ad9a9e","url":null,"abstract":"","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Surface plasmon enhanced auto-fluorescence and Raman spectroscopy for low-level detection of biological pathogens. 表面等离子体增强的自身荧光和拉曼光谱用于低水平检测生物病原体。
IF 2.4 3区 化学
Methods and Applications in Fluorescence Pub Date : 2024-12-16 DOI: 10.1088/2050-6120/ad9fd2
Anjika Panghal, Sathi Das, Pramila Thapa, Meenakshi Meenakshi, Anand Kumar, Priya Nagpal, Satish Kumar Dubey, Vivekanandan Perumal, Dalip Singh Mehta
{"title":"Surface plasmon enhanced auto-fluorescence and Raman spectroscopy for low-level detection of biological pathogens.","authors":"Anjika Panghal, Sathi Das, Pramila Thapa, Meenakshi Meenakshi, Anand Kumar, Priya Nagpal, Satish Kumar Dubey, Vivekanandan Perumal, Dalip Singh Mehta","doi":"10.1088/2050-6120/ad9fd2","DOIUrl":"https://doi.org/10.1088/2050-6120/ad9fd2","url":null,"abstract":"<p><p>The current culture-based bacterial detection technique is time-consuming and requires an extended sample preparation methodology. We propose the potential of surface-enhanced Raman spectroscopy (SERS) and surface plasmon-enhanced auto-fluorescence spectroscopy (SPEAS) for the label-free identification and quantification of bacterial pathogens at low concentrations collecting its unique auto-fluorescence and Raman signatures utilising highly anisotropic three-dimensional nanostructures of silver nano dendrites (Ag-NDs). The SERS data facilitates qualitative bacterial identification using the spectral features coming from the bacterial cell wall compound, and the SPEAS data was utilised to gain unique auto-fluorescence spectra present on the bacterial cell wall with enhanced quantification. The enhancement of Raman and auto-fluorescence signals of Ag-NDs were first evaluated using rhodamine 6g (R6G) as a probe molecule that exhibits a significant enhancement of 106 and limit of detection (LOD) of 10-12 M for SERS and 15 fold intensity enhancement and LOD of 10-15 M for SPEAS measurements. Further, the SERS and SPEAS measurements of bacterial pathogens, such as Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), using the Ag-NDs were recorded, and the results exhibit high auto-fluorescence and Raman signal intensity for both the samples up to 100 cfu/ml for both modalities. The significant photon count and distinct emission range in SPEAS measurements of bacteria enables accurate quantification. Therefore, the comprehensive investigation of plasmonic enhancement of Ag-NDs for SPEAS and SERS techniques provides complementary information about molecules to enable accurate and quick identification and quantification of pathogens.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spectral properties of 4-methylumbelliferone in PVA films; long-lived room temperature phosphorescence. 4-甲基伞形酮在PVA薄膜中的光谱性质长寿命的室温磷光。
IF 2.4 3区 化学
Methods and Applications in Fluorescence Pub Date : 2024-12-10 DOI: 10.1088/2050-6120/ad9885
Bong Lee, Emma Alexander, Danh Pham, Mariusz Gagoś, Arkadiusz Matwijczuk, Zygmunt Gryczynski, Ignacy Gryczynski
{"title":"Spectral properties of 4-methylumbelliferone in PVA films; long-lived room temperature phosphorescence.","authors":"Bong Lee, Emma Alexander, Danh Pham, Mariusz Gagoś, Arkadiusz Matwijczuk, Zygmunt Gryczynski, Ignacy Gryczynski","doi":"10.1088/2050-6120/ad9885","DOIUrl":"10.1088/2050-6120/ad9885","url":null,"abstract":"<p><p>We studied absorption and fluorescence as well as room temperature phosphorescence (RTP) of 4-methylumbelliferone (4MU) in poly (vinyl alcohol) (PVA) films. We focused our study on the long-wavelength basic form of 4MU with absorption centered at 375 nm. The strong fluorescence with a quantum yield of above 70% appears at ∼430 nm. The fluorescence anisotropy of 4MU-doped PVA film is high, reaching a value of about 0.3. The emission with gated detection shows a broad phosphorescence spectrum with a peak at ∼510 nm and a residual delayed fluorescence at 430 nm. The excitation spectra for fluorescence and phosphorescence roughly follows 4MU absorption. The phosphorescence lifetime of 4MU is remarkably long, almost 3 s. 4MU excitation and emission phosphorescence anisotropies are low, very close to zero.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142751339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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