Methods and Applications in Fluorescence最新文献_第2页

Fluorescence in depth: integration of spectroscopy and imaging with Raman, IR, and CD for advanced research. 荧光深度：光谱学和成像与拉曼、红外和 CD 的整合，用于高级研究。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-05-31 DOI: 10.1088/2050-6120/ad46e6

Lida Aeindartehran, Zahra Sadri, Fateme Rahimi, Tahereh Alinejad

引用次数: 0

Enhanced fluorescence blinking of AF647 fluorophores in Mowiol via violet and UV light induced recovery for superior localization microscopy. 通过紫光和紫外光诱导恢复，增强莫维奥中 AF647荧光团的荧光闪烁，实现卓越的定位显微镜观察。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-05-24 DOI: 10.1088/2050-6120/ad4ae6

Anupam Bharadwaj, Amalesh Kumar, Rumela Mitra, Bithiah Grace Jaganathan, Bosanta R Boruah

{"title":"Enhanced fluorescence blinking of AF647 fluorophores in Mowiol via violet and UV light induced recovery for superior localization microscopy.","authors":"Anupam Bharadwaj, Amalesh Kumar, Rumela Mitra, Bithiah Grace Jaganathan, Bosanta R Boruah","doi":"10.1088/2050-6120/ad4ae6","DOIUrl":"10.1088/2050-6120/ad4ae6","url":null,"abstract":"Blinking of fluorophores is essential in the context of single molecule localization-based optical super-resolution microscopy methods. To make the fluorescence molecule undergo blinking specific complex chemical mounting buffer systems, combined with suitable oxygen scavengers, and reducing agents are required. For instance to realise blinking in widely used fluorescence tags, like Alexa Fluor 647 (AF647), they are to be mounted on anti-fading buffer such as Mowiol and reducing agent such as Beta (β) - ME. However, the quality of the super-resolved images is decided by the total number of blinking events or in other words net duration for which the fluorescence blinking persists. In this paper we investigate how a violet and UV light induced fluorescence recovery mechanism can enhance the duration of fluorescence blinking. Our study uses AF647 dye conjugated with Phalloidin antibody in U87MG cell line mounted on Mowiol andβ- ME. On the basis of the investigation we optimize the intensity, at the sample plane, of fluorescence excitation laser at 638 nm and fluorescence recovery beam at 405 nm or in the UV giving the maximum possible fluorescence blinking duration. We observe that the longer blinking duration, using the optimized illumination scheme, has brought down the resolution in the super-resolved image, as given by Fourier Ring Correlation method, from 168 nm to 112 nm, while the separation between two nearby resolvable filaments has been brought down to ≤ 60 nm.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140916890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring light-emitting diode pumped luminescent concentrators in solid-state laser applications. 探索固体激光应用中的发光二极管泵浦发光聚光器。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-05-15 DOI: 10.1088/2050-6120/ad444b

H C S Perera, B Ford, G Das, F Balembois, J Sathian

引用次数: 0

A quinoline derivative-based supramolecular gel for fluorescence ‘turn-off’ detection of Fe3+ and Cu2+ 基于喹啉衍生物的超分子凝胶，用于荧光 "关闭 "检测 Fe3+ 和 Cu2+

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-05-02 DOI: 10.1088/2050-6120/ad4232

Shuaishuai Fu, Shang Wu, Jutao Liu, Jiajia Wang, Shuo Tian, Guangwu Zhang, Fenping Yin, Yuzhi Sun, Ping Zhang and Quanlu Yang

引用次数: 0

Resolving conjugated polymer film morphology with polarised transmission and time-resolved emission microscopy. 利用偏振透射和时间分辨发射显微镜解析共轭聚合物薄膜形态。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-04-16 DOI: 10.1088/2050-6120/ad388f

Yang Xu, Lili Sun, Kenneth P Ghiggino, Trevor A Smith

{"title":"Resolving conjugated polymer film morphology with polarised transmission and time-resolved emission microscopy.","authors":"Yang Xu, Lili Sun, Kenneth P Ghiggino, Trevor A Smith","doi":"10.1088/2050-6120/ad388f","DOIUrl":"10.1088/2050-6120/ad388f","url":null,"abstract":"The alignment of chromophores plays a crucial role in determining the optoelectronic properties of materials. Such alignment can make interpretation of fluorescence anisotropy microscopy (FAM) images somewhat ambiguous. The time-resolved emission behaviour can also influence the fluorescence anisotropy. This is particularly the case when probing excitation energy migration between chromophores in a condensed phase. Ideally information concerning the chromophoric alignment, emission decay kinetics and fluorescence anisotropy can be recorded and correlated. We report on the use of polarised transmission imaging (PTI) coupled with both steady-state and time-resolved FAM to enable accurate identification of chromophoric alignment and morphology in thin films of a conjugated polydiarylfluorene. We show that the combination of these three imaging modes presents a comprehensive methodology for investigating the alignment and morphology of chromophores in thin films, particularly for accurately mapping the distribution of amorphous and crystalline phases within the thin films, offering valuable insights for the design and optimization of materials with enhanced optoelectronic performance.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140306190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement of elements by portable x-ray fluorescence spectrometry for the study of adsorption processes: the case of Pb²⁺adsorption on soybean straw biochar. 利用便携式 X 射线荧光光谱仪测量元素以研究吸附过程：以大豆秸秆生物炭对 Pb2+ 的吸附为例。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-04-11 DOI: 10.1088/2050-6120/ad379f

Eduardo D Wannaz, Rocío Medina, Sheila D Pardo, Gonzalo P Barbero Medina, Andrés Blanco, María Julieta Salazar

{"title":"Measurement of elements by portable x-ray fluorescence spectrometry for the study of adsorption processes: the case of Pb2+adsorption on soybean straw biochar.","authors":"Eduardo D Wannaz, Rocío Medina, Sheila D Pardo, Gonzalo P Barbero Medina, Andrés Blanco, María Julieta Salazar","doi":"10.1088/2050-6120/ad379f","DOIUrl":"10.1088/2050-6120/ad379f","url":null,"abstract":"This study evaluated the reliability of portable X-ray fluorescence (pXRF) in Pb2+adsorption kinetics and isotherm experiments using soybean straw biochar. The research aimed to compare pXRF results with those obtained through traditional atomic absorption spectrometry (AAS). Soybean straw biochar, produced at 400 °C, was employed as the adsorbent for Pb2+. The efficiency of adsorption was assessed using Langmuir and Freundlich models. The kinetics of Pb2+adsorption was analysed through pseudo-first-order and pseudo-second-order models. The pseudo-second-order model described the kinetics of Pb2+adsorption on biochar better than the pseudo-first order model. Importantly, the pXRF technique demonstrated comparable results to those of AAS, making it a reliable and resource-efficient method for studying Pb2+kinetics. The results of the isotherm analyses fit the Langmuir model, indicating a desirable and irreversible adsorption of Pb2+on biochar. PXRF measurements on biochar allowed simultaneous observations of Pb2+adsorption and K+and Ca2+desorption, highlighting ionic exchange as the primary adsorption mechanism. In conclusion, our results showcased the applicability of pXRF for Pb+2adsorption studies in biochars, offering a valuable alternative to traditional methods. The findings contribute to the understanding of biochar as an effective adsorbent for heavy metals, emphasizing the potential of pXRF for cost-effective and efficient environmental research. In this study, we present a novel and detailed procedure that will allow other researchers to continue their studies on Pb2+adsorption on biochar or similar matrices, significantly reducing the resources and time used and enabling the simultaneous study of the behavior of other ions participating in the process.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140288542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective detection of ascorbic acid by tuning the composition and fluorescence of the cesium lead halide perovskite nanocrystals. 通过调节卤化铯铅过氧化物纳米晶体的成分和荧光来选择性检测抗坏血酸。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-04-09 DOI: 10.1088/2050-6120/ad3890

Jin-Zhou Liu, Rui-Xian Zhao, Qian-Wei Yin, Hai-Chi Zhang, Rong Sheng Li, Jian Ling, Qiue Cao

{"title":"Selective detection of ascorbic acid by tuning the composition and fluorescence of the cesium lead halide perovskite nanocrystals.","authors":"Jin-Zhou Liu, Rui-Xian Zhao, Qian-Wei Yin, Hai-Chi Zhang, Rong Sheng Li, Jian Ling, Qiue Cao","doi":"10.1088/2050-6120/ad3890","DOIUrl":"10.1088/2050-6120/ad3890","url":null,"abstract":"Lead halide perovskite nanocrystals (PNCs) have attracted intense attention due to their excellent optoelectronic properties. In this work, a series of water-stable CsPb(Br/I)3PNCs fluorescent probes were prepared using an anion exchange method. It was found that the PNCs probes could be used to detect ascorbic acid (AA) in water, and interestingly, the FL spectra of the PNCs probes can be adjusted by controlling the concentration of KI in anion exchange to improve the detection selectivity of AA. The high sensitivity and selectivity make CsPb(Br/I)3PNCs an ideal material for AA sensing. The concentration of AA can be linearly measured in the range from 0.01 to 50μM, with a detection limit of 4.2 nM. The reason for the enhanced FL of CsPb(Br/I)3PNCs was studied, and it is considered that AA causes the aggregation of CsPb(Br/I)3PNCs. This strategy of improving the selectivity of the probe to the substrate by adjusting the spectrum will significantly expand the application of PNCs in the field of analysis and detection.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140306191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Оn the aggregation of polycationic photosensitizer upon binding to Gram-negative bacteria. 多阳离子光敏剂与革兰氏阴性细菌结合后的聚集。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-04-05 DOI: 10.1088/2050-6120/ad3892

Igor D Romanishkin, Ekaterina V Akhlyustina, Gennady A Meerovich, Victor B Loschenov, Marina G Strakhovskaya

引用次数: 0

Portable ratiometric fluorescence detection of Cu2+ and thiram 便携式比率荧光检测 Cu2+ 和福美双

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-04-05 DOI: 10.1088/2050-6120/ad3891

Jiazhen Zhang, Yicong Jia, Xuan Tong, Hangyu Zhou, Le Zhang, Yue Yang, Xu Ji

{"title":"Portable ratiometric fluorescence detection of Cu2+ and thiram","authors":"Jiazhen Zhang, Yicong Jia, Xuan Tong, Hangyu Zhou, Le Zhang, Yue Yang, Xu Ji","doi":"10.1088/2050-6120/ad3891","DOIUrl":"https://doi.org/10.1088/2050-6120/ad3891","url":null,"abstract":"Food contaminants pose a danger to human health, but rapid, sensitive and reliable food safety detection methods can offer a solution to this problem. In this study, an optical fiber ratiometric fluorescence sensing system based on carbon dots (CDs) and o-phenylenediamine (OPD) was constructed. The ratiometric fluorescence response of Cu2+ and thiram was carried out by the fluorescence resonance energy transfer (FRET) between CDs and 2,3−diaminophenazine (ox-OPD, oxidized state o-phenylenediamine). The oxidation of OPD by Cu2+ resulted in the formation of ox-OPD, which quenched the fluorescence of CDs and exhibited a new emission peak at 573 nm. The formation of a [dithiocarbamate-Cu2+] (DTC-Cu2+) complex by reacting thiram with Cu2+, inhibits the OPD oxidation reaction triggered by Cu2+, thus turning off the fluorescence signal of OPD-Cu2+. The as-established detection system presented excellent sensitivity and selectivity for the detection of Cu2+ and thiram in the ranges of 1 ∼ 100 <italic toggle=\"yes\">μ</italic>M and 5 ∼ 50 <italic toggle=\"yes\">μ</italic>M, respectively. The lowest detection limits were 0.392 <italic toggle=\"yes\">μ</italic>M for Cu2+ and 0.522 <italic toggle=\"yes\">μ</italic>M for thiram. Furthermore, actual sample analysis indicated that the sensor had the potential for Cu2+ and thiram assays in real sample analysis.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"57 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140610195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Pr³⁺concentration in luminescence properties & upconversion mechanism of triple doped NaYF₄: Yb³⁺, Er³⁺, Pr³. Pr3+ 浓度对 Yb3+、Er3+、Pr3 三重掺杂 NaYF4 发光特性和上转换机制的影响。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-03-26 DOI: 10.1088/2050-6120/ad326e

Mahla Shahsavar Gocmen, Ayse Dulda

{"title":"Effect of Pr3+concentration in luminescence properties & upconversion mechanism of triple doped NaYF4: Yb3+, Er3+, Pr3.","authors":"Mahla Shahsavar Gocmen, Ayse Dulda","doi":"10.1088/2050-6120/ad326e","DOIUrl":"10.1088/2050-6120/ad326e","url":null,"abstract":"Lanthanide-doped fluoride nanocrystals (NCs) exhibit excellent optical features, including upconversion and downconversion luminescence (UCL and DCL), that can be utilized in a variety of applications. In this study, we have successfully demonstrated the photoluminescence behavior of triple-doped NaYF4: Yb3+, Er3+, Pr3+NCs in the Vis-NIR region. Herein, highly monodisperse hexagonal phase NaYF4: Yb0.2, Er0.02, Prxnanocrystals in various Pr3+(x = 0, 0.1, 0.5, and 1 mol %) concentration with ∼22 nm diameter synthesized by thermal decomposition technique. The photoluminescence studies for all samples were performed under 980 nm laser excitation. The luminescence intensity of Er3+including blue (407 nm), green (520 and 540 nm), red (654 nm), and near-infrared (845 nm and 1530 nm) emissions was significantly quenched and Pr3+emission intensity at 1290 nm (Pr3+:1G4→3H5) changes irregularly upon doping with Pr3+ions. Furthermore, we performed the excitation power dependence and decay time analysis to investigate the energy transfer and upconversion mechanisms of samples. These findings indicate that the presence of praseodymium strongly reduces emission intensities due to abundant cross-relaxation channels. In addition, particle size is an efficient factor, shedding light on the influence of Pr3+on the energy transfer and upconversion mechanisms of the fluorides.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0