Methods and Applications in Fluorescence最新文献_第2页

Room temperature luminescence of a triangulenium dye ADOTA in PVA films. 三角形染料ADOTA在PVA薄膜中的室温发光特性。

IF 2.4 3区化学

Methods and Applications in Fluorescence Pub Date : 2026-03-03 DOI: 10.1088/2050-6120/ae484d

Zygmunt Gryczynski, Agnieszka Jablonska, Bong Lee, Bo W Laursen, Thomas Just Sørensen, Danh Pham, Rajveer Sagoo, Trang Thien Pham, Ignacy Gryczynski

引用次数: 0

Crude oil density prediction and visualization by CLSM fluorescence spectroscopy combined with chemometric methods. CLSM荧光光谱与化学计量相结合的原油密度预测与可视化。

IF 2.4 3区化学

Methods and Applications in Fluorescence Pub Date : 2026-03-02 DOI: 10.1088/2050-6120/ae48ab

Wenjie Yang, Tiefeng Lin, Jinyou Zhang, Xianda Sun, Chengwu Xu, Ling Zhao, Baizhou Fu, Yue Zhou, Dejiang Kang, Yan Wang, Wei Wu, Tian Tian

{"title":"Crude oil density prediction and visualization by CLSM fluorescence spectroscopy combined with chemometric methods.","authors":"Wenjie Yang, Tiefeng Lin, Jinyou Zhang, Xianda Sun, Chengwu Xu, Ling Zhao, Baizhou Fu, Yue Zhou, Dejiang Kang, Yan Wang, Wei Wu, Tian Tian","doi":"10.1088/2050-6120/ae48ab","DOIUrl":"10.1088/2050-6120/ae48ab","url":null,"abstract":"This study presents a metrologically traceable, microscale measurement framework integrating Confocal Laser Scanning Microscopy (CLSM), fluorescence spectroscopy, and chemometric modeling for quantitative crude oil density mapping. Thirty-three samples from the Songliao Basin (0.764-0.9655 g cm-3) were analyzed under 488 nm excitation with emission spectra collected from 500-794 nm. Baseline correction, smoothing, and normalization improved spectral consistency, while principal component analysis with Hotelling's T2removed two outliers prior to modeling. Support Vector Regression (SVR) outperformed Partial Least Squares Regression (PLSR), achieving a calibration R2_calof 0.968 (RMSEC = 0.008) and a prediction R2_preof 0.955 (RMSEP = 0.012). The 737 nm wavelength exhibited the strongest correlation (r= 0.748, Pearson correlation coefficient) with aromatic and asphaltene content, linking molecular composition to density variation. Applied to CLSM spectral images of shale thin sections, the SVR model produced micrometre-scale density maps consistent with compositional heterogeneity-light fractions along fracture walls and heavy fractions within fracture interiors. A standalone desktop tool has been developed to enable spectral import, automated processing, and density visualization. This non-destructive, high-resolution approach addresses the limitations of bulk measurements, enabling reproducible, spatially resolved density determination in complex geological matrices and advancing measurement science.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146258689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectral properties of quinine sulfate in PVA films for front-face format emission measurements. 硫酸奎宁在PVA薄膜中的光谱特性及其正面发射测量。

IF 2.4 3区化学

Methods and Applications in Fluorescence Pub Date : 2026-02-27 DOI: 10.1088/2050-6120/ae47bd

Agnieszka Jablonska, Bong Lee, R Max Petty, Danh Pham, Rajveer Sagoo, Trang Thien Pham, Zygmunt Gryczynski, Ignacy Gryczynski

引用次数: 0

Four-membered N-heterocyclic carbenes in carbene metal amide emitters: a quantum chemical view. 卡宾金属酰胺发射体中的四元n杂环卡宾：量子化学观点。

IF 2.4 3区化学

Methods and Applications in Fluorescence Pub Date : 2026-02-24 DOI: 10.1088/2050-6120/ae428d

Jasper Guhl, Tu Viet Chu, Tobias Kretschmer, Leonard Karl, Christian Ganter, Christel M Marian

{"title":"Four-membered N-heterocyclic carbenes in carbene metal amide emitters: a quantum chemical view.","authors":"Jasper Guhl, Tu Viet Chu, Tobias Kretschmer, Leonard Karl, Christian Ganter, Christel M Marian","doi":"10.1088/2050-6120/ae428d","DOIUrl":"10.1088/2050-6120/ae428d","url":null,"abstract":"Using computational chemistry, we have scanned a set of four-membered N-heterocyclic carbenes with bulky substituents for their ability to form carbene metal amides (CMAs) with excellent thermally activated delayed fluorescence (TADF) properties. In comparison to the properties of their well-known five- and six-membered analogs, the transition dipole moments of the first excited singlet states of the corresponding Cu(I)carbazolide (Cz) complexes increase. For CMAs of the most promising four-membered carbene, a lactam-based carbene (4LAC), detailed investigations of the TADF properties have been performed using advanced quantum chemical methods. Due to the small energy separation between its singlet and triplet ligand-to-ligand charge-transfer (LLCT) states, 4LAC-Ag(I)-Cz exhibits the best ratio between reverse intersystem crossing (rISC) and intersystem crossing in the coinage metal triad for a coplanar orientation of the ligands. The TADF properties of the corresponding Cu(I)and Au(I)complexes benefit from twisted ligand-ligand alignments, achieved by using tetrafluorocarbazolide (4FCz) as donor ligand. The moderate reduction of the fluorescence rate constant upon twisting by about 45-50° is overcompensated by a decrease of the singlet-triplet energy gap, thus improving the TADF performance. Overall, with fluorescence rate constants of the order of 107s-1and rISC rate constants between 109and 1010s-1, TADF should have competitive advantage over common triplet deactivation processes such as triplet-triplet annihilation. Like in other CMAs, full excited-state geometry relaxation in liquid solution is detrimental for the emission properties. In the solid state, where the formation of a perpendicular ligand-ligand alignment is sterically hindered by the environment, 4LAC-M-Cz and 4LAC-M-4FCz are predicted to be efficient TADF compounds with red to orange emission.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146125600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growing the family of fluorescence. 培养荧光族。

IF 2.4 3区化学

Methods and Applications in Fluorescence Pub Date : 2026-01-21 DOI: 10.1088/2050-6120/ae3661

David J S Birch, Marcia Levitus, Yves Mély

引用次数: 0

Mechanistic basis for 2-aminopurine quenching by morpholine- and piperazine-based Good's buffers. 基于吗啡啉和哌嗪的Good’s缓冲液猝灭2-氨基嘌呤的机理基础。

IF 2.4 3区化学

Methods and Applications in Fluorescence Pub Date : 2026-01-20 DOI: 10.1088/2050-6120/ae35d0

Souvik Poddar, Benjamin Glennon, Marcia Levitus

{"title":"Mechanistic basis for 2-aminopurine quenching by morpholine- and piperazine-based Good's buffers.","authors":"Souvik Poddar, Benjamin Glennon, Marcia Levitus","doi":"10.1088/2050-6120/ae35d0","DOIUrl":"10.1088/2050-6120/ae35d0","url":null,"abstract":"2-Aminopurine (2AP) is the most widely used fluorescent nucleobase analog in DNA and RNA research. While quenching of 2AP by DNA bases has been extensively characterized, the effect of extrinsic quenchers has received far less attention. This study examines the fluorescence quenching mechanisms of 2AP by commonly used buffers in biochemical research. We systematically investigated four Good's buffers-MES, MOPS, HEPES, and PIPES-along with their parent compounds morpholine and piperazine across a range of pH conditions and concentrations. For morpholine-containing buffers (MES and MOPS), quenching occurs predominantly at pH values at or above their respective pKa values and is negligible at more than two pH units below the pKa. In contrast, piperazine-containing buffers (HEPES and PIPES) exhibit substantial quenching even below their pKa values due to the presence of two basic nitrogen atoms in the piperazine ring, one of which remains unprotonated and reactive across the investigated pH range. Time-resolved fluorescence measurements demonstrate that quenching is primarily dynamic for MES, MOPS, and HEPES, while PIPES shows significant static quenching contributions. Results are consistent with a mechanism involving photoinduced electron transfer from unprotonated tertiary amines to excited-state 2AP. The thermodynamic feasibility of this mechanism is supported by the low oxidation potentials of these tertiary amines compared to primary amine-containing buffers such as TRIS, which does not quench 2AP fluorescence. These results have significant practical implications for fluorescence-based studies using 2AP as a structural or dynamic probe in nucleic acid research. Buffer selection can substantially alter both quantum yields and fluorescence lifetimes of 2AP, potentially leading to misinterpretation of experimental data if these effects are not properly accounted for.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145934037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beam shaping on a fluorescent on-chip imaging system. 荧光片上成像系统的光束整形。

IF 2.4 3区化学

Methods and Applications in Fluorescence Pub Date : 2026-01-08 DOI: 10.1088/2050-6120/ae2b4d

Çağlar Arpali, Serap Altay Arpali

{"title":"Beam shaping on a fluorescent on-chip imaging system.","authors":"Çağlar Arpali, Serap Altay Arpali","doi":"10.1088/2050-6120/ae2b4d","DOIUrl":"10.1088/2050-6120/ae2b4d","url":null,"abstract":"The fluorescent on-chip imaging system differs from a conventional fluorescent microscope in terms of the imaging method because the sample is directly placed on the imaging sensor (i.e., charge-coupled device (CCD)). While this imaging modality presents several advantages, including a wide field of view and rapid scanning speed, it can be difficult to detect certain particles in dense and scattering environments, such as whole blood and tissue. These difficulties lead to a decreased signal-to-noise ratio (SNR) in the captured images, influenced by both the medium's light-transmitting capability and the excitation techniques used. In this paper, we quantitatively examine the effect of beam shaping techniques on a fluorescent on-chip imaging system from the SNR perspective. An experimental comparison is conducted between a Gaussian beam and plane-wave illumination generated by a novel phase modulation schema using our developed imaging platform. The results indicate that the Gaussian beam produces higher SNR images than plane waves when detecting fluorescent particles in a microchannel. Gaussian beam's higher energy confinement ability enhances the image quality of on-chip fluorescent imaging systems, particularly involving scattering-like medium limitations.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145724550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uranium speciation by time-resolved laser-induced fluorescence spectroscopy. 时间分辨激光诱导荧光光谱法测定铀的形态。

IF 2.4 3区化学

Methods and Applications in Fluorescence Pub Date : 2025-12-09 DOI: 10.1088/2050-6120/ae2566

Alena Zavadilová, Martin Daňo, Zdeněk Zapletálek, David Vyhlídal

{"title":"Uranium speciation by time-resolved laser-induced fluorescence spectroscopy.","authors":"Alena Zavadilová, Martin Daňo, Zdeněk Zapletálek, David Vyhlídal","doi":"10.1088/2050-6120/ae2566","DOIUrl":"10.1088/2050-6120/ae2566","url":null,"abstract":"Uranium(VI) speciation in aqueous carbonate solutions was systematically investigated using time-resolved laser-induced fluorescence spectroscopy (TRLFS) across a broad pH range (4.3-13.0) at room temperature. Distinct uranyl complexes were identified based on their luminescence lifetimes and emission spectra, and their formation was correlated with the theoretical speciation models. Particular emphasis was placed on alkaline conditions, where uranium speciation is less understood due to weak luminescence signals. This study revealed the presence of multiple hydroxo and carbonato complexes, including non-luminescent species at high pH. These findings provide new insights into uranium(VI) behaviour in cementitious environments relevant to deep geological repositories. Moreover the dynamics of complex formation were investigated, and the quantity of precipitates were quantified using the methods based on the luminescent properties of uranium and the presence of a complexing agent. The luminescence intensity was shown to be independent of pH and linearly correlated with uranium concentration, confirming TRLFS as a robust tool for uranium quantification in variable geochemical settings. This work contributes to a more accurate understanding of uranium mobility and stability in nuclear waste management scenarios and in locations contaminated with elevated uranium levels as a resulting of ore extraction or processing.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145635758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A call to action: democratizing single molecule fluorescence microscopy. 行动呼吁：使单分子荧光显微镜大众化。

IF 2.4 3区化学

Methods and Applications in Fluorescence Pub Date : 2025-11-05 DOI: 10.1088/2050-6120/ae1928

Rutuparna Kulkarni, Thorsten Wohland

{"title":"A call to action: democratizing single molecule fluorescence microscopy.","authors":"Rutuparna Kulkarni, Thorsten Wohland","doi":"10.1088/2050-6120/ae1928","DOIUrl":"10.1088/2050-6120/ae1928","url":null,"abstract":"Fluorescence-based optical techniques are developing rapidly, giving access to high spatiotemporal information on live biological systems with single molecule sensitivity. However, these techniques are typically restricted to expert labs and are not easilyaccessibleto the general user. While the development of customized systems and their wider distribution is difficult, as it requires expert manpower, software developments are easy to distribute. However, in reality only few users outside an expert community are exploring and using these tools. This is due to theusabilityof the software which often requires expert skills to operate and is neither intuitive nor easy to use. These issues of accessibility and usability limit the spread of state-of-the-art techniques. And while accessibility of custom instrumentation is difficult to solve, the accessibility and usability of software is an easier target. In this perspective, therefore, we concentrate on the software issue and examine the major translational barriers that prevent biologists from adopting the available fluorescence microscopy techniques. We discuss key developments in the field such as open-source tools, standardized file formats and AI-driven analysis platforms, and suggest a roadmap to bring advanced tools to a wider community.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and limitations of molecular resolution fluorescence imaging. 分子分辨率荧光成像的挑战和局限性。

IF 2.4 3区化学

Methods and Applications in Fluorescence Pub Date : 2025-09-17 DOI: 10.1088/2050-6120/ae042b

Dominic A Helmerich, Markus Sauer

{"title":"Challenges and limitations of molecular resolution fluorescence imaging.","authors":"Dominic A Helmerich, Markus Sauer","doi":"10.1088/2050-6120/ae042b","DOIUrl":"10.1088/2050-6120/ae042b","url":null,"abstract":"Super-resolution microscopy (SRM) has revolutionized fluorescence imaging enabling insights into the molecular organization of cells that were previously unconceivable. Latest developments now allow the visualization of individual molecules with nanometer precision and imaging with molecular resolution. However, translating these achievements to imaging under physiological conditions in cells remains challenging. The higher the spatial resolution is pushed by the development of improved SRM methods the more challenging the problems we are confronted when aiming to use them for sub-10 nm fluorescence imaging in cells. It turns out that most developed SRM methods that demonstrate nanometer resolution cannot be directly implemented for molecular resolution imaging in cells. Particularly, fluorescence labeling, i.e. high-density covalent labeling of the molecules of interest with fluorophores with minimal linkage error represents currently a nearly insurmountable obstacle. In addition, even if high labeling densities can be realized it has to be considered that fluorophores can interact via different energy pathways and thus impede super-resolution imaging in the sub-10 nm range. Here, we describe the boundaries, discuss the challenges we must accept and show strategies to circumvent them and achieve true molecular resolution fluorescence imaging under physiological conditions in cells.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0