{"title":"A quinoline derivative-based supramolecular gel for fluorescence ‘turn-off’ detection of Fe3+ and Cu2+","authors":"Shuaishuai Fu, Shang Wu, Jutao Liu, Jiajia Wang, Shuo Tian, Guangwu Zhang, Fenping Yin, Yuzhi Sun, Ping Zhang and Quanlu Yang","doi":"10.1088/2050-6120/ad4232","DOIUrl":"https://doi.org/10.1088/2050-6120/ad4232","url":null,"abstract":"In this research, we synthesized and constructed a novel gelator (named QN) combining quinoline and naphthalene that self-assembled in N, N-dimethylformamide (DMF) to form a stable supramolecular gel (named OQN). Under UV light, gel OQN exhibited extremely bright yellow fluorescence. The gel OQN showed excellent sensing performance for both Fe3+ and Cu2+, with a fluorescence ‘turn-off’ detection mechanism and the lowest detection limit of 7.58 × 10−8 M and 1.51 × 10−8 M, respectively. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectra, x-ray powder diffraction (XRD), rheological measurements, x-ray photoelectron spectroscopy (XPS), and fluorescence spectroscopy were used to characterize the gel OQN. The OQN ion-responsive membrane created is an excellent fluorescent writing material.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"17 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140839464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yang Xu, Lili Sun, Kenneth P Ghiggino, Trevor A Smith
{"title":"Resolving conjugated polymer film morphology with polarised transmission and time-resolved emission microscopy.","authors":"Yang Xu, Lili Sun, Kenneth P Ghiggino, Trevor A Smith","doi":"10.1088/2050-6120/ad388f","DOIUrl":"10.1088/2050-6120/ad388f","url":null,"abstract":"<p><p>The alignment of chromophores plays a crucial role in determining the optoelectronic properties of materials. Such alignment can make interpretation of fluorescence anisotropy microscopy (FAM) images somewhat ambiguous. The time-resolved emission behaviour can also influence the fluorescence anisotropy. This is particularly the case when probing excitation energy migration between chromophores in a condensed phase. Ideally information concerning the chromophoric alignment, emission decay kinetics and fluorescence anisotropy can be recorded and correlated. We report on the use of polarised transmission imaging (PTI) coupled with both steady-state and time-resolved FAM to enable accurate identification of chromophoric alignment and morphology in thin films of a conjugated polydiarylfluorene. We show that the combination of these three imaging modes presents a comprehensive methodology for investigating the alignment and morphology of chromophores in thin films, particularly for accurately mapping the distribution of amorphous and crystalline phases within the thin films, offering valuable insights for the design and optimization of materials with enhanced optoelectronic performance.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140306190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eduardo D Wannaz, Rocío Medina, Sheila D Pardo, Gonzalo P Barbero Medina, Andrés Blanco, María Julieta Salazar
{"title":"Measurement of elements by portable x-ray fluorescence spectrometry for the study of adsorption processes: the case of Pb<sup>2+</sup>adsorption on soybean straw biochar.","authors":"Eduardo D Wannaz, Rocío Medina, Sheila D Pardo, Gonzalo P Barbero Medina, Andrés Blanco, María Julieta Salazar","doi":"10.1088/2050-6120/ad379f","DOIUrl":"10.1088/2050-6120/ad379f","url":null,"abstract":"<p><p>This study evaluated the reliability of portable X-ray fluorescence (pXRF) in Pb<sup>2+</sup>adsorption kinetics and isotherm experiments using soybean straw biochar. The research aimed to compare pXRF results with those obtained through traditional atomic absorption spectrometry (AAS). Soybean straw biochar, produced at 400 °C, was employed as the adsorbent for Pb<sup>2+</sup>. The efficiency of adsorption was assessed using Langmuir and Freundlich models. The kinetics of Pb<sup>2+</sup>adsorption was analysed through pseudo-first-order and pseudo-second-order models. The pseudo-second-order model described the kinetics of Pb<sup>2+</sup>adsorption on biochar better than the pseudo-first order model. Importantly, the pXRF technique demonstrated comparable results to those of AAS, making it a reliable and resource-efficient method for studying Pb<sup>2+</sup>kinetics. The results of the isotherm analyses fit the Langmuir model, indicating a desirable and irreversible adsorption of Pb<sup>2+</sup>on biochar. PXRF measurements on biochar allowed simultaneous observations of Pb<sup>2+</sup>adsorption and K<sup>+</sup>and Ca<sup>2+</sup>desorption, highlighting ionic exchange as the primary adsorption mechanism. In conclusion, our results showcased the applicability of pXRF for Pb<sup>+2</sup>adsorption studies in biochars, offering a valuable alternative to traditional methods. The findings contribute to the understanding of biochar as an effective adsorbent for heavy metals, emphasizing the potential of pXRF for cost-effective and efficient environmental research. In this study, we present a novel and detailed procedure that will allow other researchers to continue their studies on Pb<sup>2+</sup>adsorption on biochar or similar matrices, significantly reducing the resources and time used and enabling the simultaneous study of the behavior of other ions participating in the process.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140288542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jin-Zhou Liu, Rui-Xian Zhao, Qian-Wei Yin, Hai-Chi Zhang, Rong Sheng Li, Jian Ling, Qiue Cao
{"title":"Selective detection of ascorbic acid by tuning the composition and fluorescence of the cesium lead halide perovskite nanocrystals.","authors":"Jin-Zhou Liu, Rui-Xian Zhao, Qian-Wei Yin, Hai-Chi Zhang, Rong Sheng Li, Jian Ling, Qiue Cao","doi":"10.1088/2050-6120/ad3890","DOIUrl":"10.1088/2050-6120/ad3890","url":null,"abstract":"<p><p>Lead halide perovskite nanocrystals (PNCs) have attracted intense attention due to their excellent optoelectronic properties. In this work, a series of water-stable CsPb(Br/I)<sub>3</sub>PNCs fluorescent probes were prepared using an anion exchange method. It was found that the PNCs probes could be used to detect ascorbic acid (AA) in water, and interestingly, the FL spectra of the PNCs probes can be adjusted by controlling the concentration of KI in anion exchange to improve the detection selectivity of AA. The high sensitivity and selectivity make CsPb(Br/I)<sub>3</sub>PNCs an ideal material for AA sensing. The concentration of AA can be linearly measured in the range from 0.01 to 50<i>μ</i>M, with a detection limit of 4.2 nM. The reason for the enhanced FL of CsPb(Br/I)<sub>3</sub>PNCs was studied, and it is considered that AA causes the aggregation of CsPb(Br/I)<sub>3</sub>PNCs. This strategy of improving the selectivity of the probe to the substrate by adjusting the spectrum will significantly expand the application of PNCs in the field of analysis and detection.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140306191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Igor D Romanishkin, Ekaterina V Akhlyustina, Gennady A Meerovich, Victor B Loschenov, Marina G Strakhovskaya
{"title":"Оn the aggregation of polycationic photosensitizer upon binding to Gram-negative bacteria.","authors":"Igor D Romanishkin, Ekaterina V Akhlyustina, Gennady A Meerovich, Victor B Loschenov, Marina G Strakhovskaya","doi":"10.1088/2050-6120/ad3892","DOIUrl":"10.1088/2050-6120/ad3892","url":null,"abstract":"<p><p>Polycationic photosensitizers (PS) are not susceptible to aggregation in solutions, but their high local concentrations in Gram-negative bacteria can be sufficient for aggregation and reduced effectiveness of antibacterial photodynamic treatment. By measuring fluorescence spectra and kinetics we were able to evaluate the degree of aggregation of polycationic PS ZnPcChol<sub>8</sub>in Gram-negative bacteria E.<i>coli</i>K12 TG1. Binding of ZnPcChol<sub>8</sub>to<i>E</i>.<i>coli</i>K12 TG1 leads to an appearance of groups of molecules with shorter PS fluorescence lifetime, a decrease in fluorescence intensity and a shift in the fluorescence spectral maximum. However, we evaluated that about 88% of the fluorescing PS molecules in the bacteria were in an unaggregated state, which indicates only a small reduction in the generation of reactive oxygen species.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140306192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jiazhen Zhang, Yicong Jia, Xuan Tong, Hangyu Zhou, Le Zhang, Yue Yang, Xu Ji
{"title":"Portable ratiometric fluorescence detection of Cu2+ and thiram","authors":"Jiazhen Zhang, Yicong Jia, Xuan Tong, Hangyu Zhou, Le Zhang, Yue Yang, Xu Ji","doi":"10.1088/2050-6120/ad3891","DOIUrl":"https://doi.org/10.1088/2050-6120/ad3891","url":null,"abstract":"Food contaminants pose a danger to human health, but rapid, sensitive and reliable food safety detection methods can offer a solution to this problem. In this study, an optical fiber ratiometric fluorescence sensing system based on carbon dots (CDs) and o-phenylenediamine (OPD) was constructed. The ratiometric fluorescence response of Cu<sup>2+</sup> and thiram was carried out by the fluorescence resonance energy transfer (FRET) between CDs and 2,3−diaminophenazine (ox-OPD, oxidized state o-phenylenediamine). The oxidation of OPD by Cu<sup>2+</sup> resulted in the formation of ox-OPD, which quenched the fluorescence of CDs and exhibited a new emission peak at 573 nm. The formation of a [dithiocarbamate-Cu<sup>2+</sup>] (DTC-Cu<sup>2+</sup>) complex by reacting thiram with Cu<sup>2+</sup>, inhibits the OPD oxidation reaction triggered by Cu<sup>2+</sup>, thus turning off the fluorescence signal of OPD-Cu<sup>2+</sup>. The as-established detection system presented excellent sensitivity and selectivity for the detection of Cu<sup>2+</sup> and thiram in the ranges of 1 ∼ 100 <italic toggle=\"yes\">μ</italic>M and 5 ∼ 50 <italic toggle=\"yes\">μ</italic>M, respectively. The lowest detection limits were 0.392 <italic toggle=\"yes\">μ</italic>M for Cu<sup>2+</sup> and 0.522 <italic toggle=\"yes\">μ</italic>M for thiram. Furthermore, actual sample analysis indicated that the sensor had the potential for Cu<sup>2+</sup> and thiram assays in real sample analysis.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"57 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140610195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Pr<sup>3+</sup>concentration in luminescence properties & upconversion mechanism of triple doped NaYF<sub>4</sub>: Yb<sup>3+</sup>, Er<sup>3+</sup>, Pr<sup>3</sup>.","authors":"Mahla Shahsavar Gocmen, Ayse Dulda","doi":"10.1088/2050-6120/ad326e","DOIUrl":"10.1088/2050-6120/ad326e","url":null,"abstract":"<p><p>Lanthanide-doped fluoride nanocrystals (NCs) exhibit excellent optical features, including upconversion and downconversion luminescence (UCL and DCL), that can be utilized in a variety of applications. In this study, we have successfully demonstrated the photoluminescence behavior of triple-doped NaYF<sub>4</sub>: Yb<sup>3+</sup>, Er<sup>3+</sup>, Pr<sup>3+</sup>NCs in the Vis-NIR region. Herein, highly monodisperse hexagonal phase NaYF<sub>4</sub>: Yb<sub>0.2</sub>, Er<sub>0.02</sub>, Pr<sub>x</sub>nanocrystals in various Pr<sup>3+</sup>(x = 0, 0.1, 0.5, and 1 mol %) concentration with ∼22 nm diameter synthesized by thermal decomposition technique. The photoluminescence studies for all samples were performed under 980 nm laser excitation. The luminescence intensity of Er<sup>3+</sup>including blue (407 nm), green (520 and 540 nm), red (654 nm), and near-infrared (845 nm and 1530 nm) emissions was significantly quenched and Pr<sup>3+</sup>emission intensity at 1290 nm (Pr<sup>3+</sup>:<sup>1</sup>G<sub>4</sub>→<sup>3</sup>H<sub>5</sub>) changes irregularly upon doping with Pr<sup>3+</sup>ions. Furthermore, we performed the excitation power dependence and decay time analysis to investigate the energy transfer and upconversion mechanisms of samples. These findings indicate that the presence of praseodymium strongly reduces emission intensities due to abundant cross-relaxation channels. In addition, particle size is an efficient factor, shedding light on the influence of Pr<sup>3+</sup>on the energy transfer and upconversion mechanisms of the fluorides.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Matías E Carranza, Hugo M Elero, Pedro J Peña Hernández, Alicia V Veglia
{"title":"Calixarenes and cyclodextrins as off- and on-fluorescence probes for carbazole.","authors":"Matías E Carranza, Hugo M Elero, Pedro J Peña Hernández, Alicia V Veglia","doi":"10.1088/2050-6120/ad326d","DOIUrl":"10.1088/2050-6120/ad326d","url":null,"abstract":"<p><p>Absorption and fluorescence spectra of the nitrogen polycyclic aromatic hydrocarbon carbazole (<b>CZL</b>) were analyzed with native cyclodextrins (<b>CD</b>;<i>α</i>,<i>β</i>,<i>γ</i>); derivatized<b>CD</b>(hydroxypropyl-<i>β</i>-cyclodextrin,<b>HPCD</b>; methyl-<i>β</i>-cyclodextrin,<b>MeCD</b>) and p-sulfonated calixarenes (<b>SCAn</b>, with n = 6 and 8) macrocycles. The results showed a slight increase in the absorbance of<b>CZL</b>with<b>CD</b>, but the mixture of<b>CZL</b>with<b>SCA</b>showed lower absorption than the sum of the individual spectra. Also, changes in fluorescence were observed by adding the macrocycles, quenching with<b>SCA</b>, and significant increases with<b>CD</b>. The higher fluorescence enhancement was with<b>HPCD</b>rationalized as a complex formation with 1:1 stoichiometry, with an average value for the association constant (<i>K</i><sub><i>A</i></sub>) of (12 ± 1) x 10<sup>2</sup>M<sup>-1</sup>, and a quantum yield ratio between the complexed<b>CZL</b>and free<b>CZL</b>(Φ<sup>CZL-HPCD</sup>/Φ<sup>CZL</sup>) of (1.56 ± 0.02) at neutral pH and 25.0 °C. These increases in fluorescence were used as an on-fluorescence switch to develop a supramolecular analytical method for<b>CZL</b>in aqueous samples. The best analytical parameters were in<b>HPCD</b>(LOD = 1.41 ± 0.01 ng mL<sup>-1</sup>). The method was validated in aqueous samples of river and tap water with recoveries between 96%-104%. The proposed supramolecular method is quick, direct, selective and represents an alternative and low-cost analysis method.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Improving STED microscopy with SUPPOSe: enhancing resolution from a single-image.","authors":"Micaela Toscani, Axel M Lacapmesure","doi":"10.1088/2050-6120/ad31b8","DOIUrl":"10.1088/2050-6120/ad31b8","url":null,"abstract":"<p><p>Here we apply the SUPPOSe algorithm on images acquired using Stimulated Emission Depletion (STED) microscopy with the aim of improving the resolution limit achieved. We processed images of the nuclear pore complex (NPC) from cell lines in which the Nup96 nucleoporin was endogenously labeled. This reference protein forms a ring whose diameter is ∼107 nm with 8 corners ∼42 nm apart from each other. The stereotypic arrangement of proteins in the NPC has been used as reference structures to characterize the performance of a variety of microscopy techniques. STED microscopy images resolve the ring arrangement but not the eightfold symmetry of the NPC. After applying the SUPPOSe algorithm to the STED images, we were able to solve the octagonal structure of the NPC. After processing 562 single NPC, the average radius of the NPC was found to be<i>R</i>= 54.2 ± 2.9 nm, being consistent with the theoretical distances of this structure. To verify that the solutions obtained are compatible with a NPC-type geometry, we rotate the solutions to optimally fit an eightfold-symmetric pattern and we count the number of corners that contain at least one localization. Fitting a probabilistic model to the histogram of the number of bright corners gives an effective labeling efficiency of 31%, which is in agreement with the values reported in for other cell lines and ligands used in Single Molecule Localization microscopy, showing that SUPPOSe can reliably retrieve sub-resolution, nanoscale objects from single acquisitions even in noisy conditions.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140065499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Formation of gel and solid phases in acrylic cuvettes upon exposure to DMSO, oxygen and light: implications for fluorescence spectroscopy.","authors":"Alain Bolaño Alvarez, Steffen B Petersen","doi":"10.1088/2050-6120/ad2f39","DOIUrl":"10.1088/2050-6120/ad2f39","url":null,"abstract":"<p><p>We here report the formation of a turbid-gel phase in acrylic cuvettes upon exposure to pure Dimethyl Sulfoxide (DMSO) at room temperature. The observed phenomenon occurred over a 10 h to 14 h incubation in the presence of environmental oxygen. After the turbid gel was removed from the cuvette, it became a white solid exhibiting unique emission behavior. The formation of the turbid-gel phase was accelerated upon exposure to UV 295 LED pulses of light from 6 h to 8 h. Surprisingly, subsequent exposure of the white solid to a few microliters of pure DMSO and vortexing resulted in its transformation into a transparent gel state in just a few minutes, eventually acquiring transparent and liquid properties. Additionally, the white-solid phase can load other molecules, such as Resveratrol and Quercetin, leading to shifts in the respective emission spectra compared with the same molecule in liquid and pure DMSO. These novel findings highlight the interaction between UV photons, oxygen, DMSO and Acrylic, and potentially distort fluorescence spectroscopy experiments.</p>","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}