Methods and Applications in Fluorescence最新文献_第3页

Оn the aggregation of polycationic photosensitizer upon binding to Gram-negative bacteria. 多阳离子光敏剂与革兰氏阴性细菌结合后的聚集。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-04-05 DOI: 10.1088/2050-6120/ad3892

Igor D Romanishkin, Ekaterina V Akhlyustina, Gennady A Meerovich, Victor B Loschenov, Marina G Strakhovskaya

引用次数: 0

Portable ratiometric fluorescence detection of Cu2+ and thiram 便携式比率荧光检测 Cu2+ 和福美双

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-04-05 DOI: 10.1088/2050-6120/ad3891

Jiazhen Zhang, Yicong Jia, Xuan Tong, Hangyu Zhou, Le Zhang, Yue Yang, Xu Ji

{"title":"Portable ratiometric fluorescence detection of Cu2+ and thiram","authors":"Jiazhen Zhang, Yicong Jia, Xuan Tong, Hangyu Zhou, Le Zhang, Yue Yang, Xu Ji","doi":"10.1088/2050-6120/ad3891","DOIUrl":"https://doi.org/10.1088/2050-6120/ad3891","url":null,"abstract":"Food contaminants pose a danger to human health, but rapid, sensitive and reliable food safety detection methods can offer a solution to this problem. In this study, an optical fiber ratiometric fluorescence sensing system based on carbon dots (CDs) and o-phenylenediamine (OPD) was constructed. The ratiometric fluorescence response of Cu2+ and thiram was carried out by the fluorescence resonance energy transfer (FRET) between CDs and 2,3−diaminophenazine (ox-OPD, oxidized state o-phenylenediamine). The oxidation of OPD by Cu2+ resulted in the formation of ox-OPD, which quenched the fluorescence of CDs and exhibited a new emission peak at 573 nm. The formation of a [dithiocarbamate-Cu2+] (DTC-Cu2+) complex by reacting thiram with Cu2+, inhibits the OPD oxidation reaction triggered by Cu2+, thus turning off the fluorescence signal of OPD-Cu2+. The as-established detection system presented excellent sensitivity and selectivity for the detection of Cu2+ and thiram in the ranges of 1 ∼ 100 <italic toggle=\"yes\">μ</italic>M and 5 ∼ 50 <italic toggle=\"yes\">μ</italic>M, respectively. The lowest detection limits were 0.392 <italic toggle=\"yes\">μ</italic>M for Cu2+ and 0.522 <italic toggle=\"yes\">μ</italic>M for thiram. Furthermore, actual sample analysis indicated that the sensor had the potential for Cu2+ and thiram assays in real sample analysis.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"57 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140610195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Pr³⁺concentration in luminescence properties & upconversion mechanism of triple doped NaYF₄: Yb³⁺, Er³⁺, Pr³. Pr3+ 浓度对 Yb3+、Er3+、Pr3 三重掺杂 NaYF4 发光特性和上转换机制的影响。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-03-26 DOI: 10.1088/2050-6120/ad326e

Mahla Shahsavar Gocmen, Ayse Dulda

{"title":"Effect of Pr3+concentration in luminescence properties & upconversion mechanism of triple doped NaYF4: Yb3+, Er3+, Pr3.","authors":"Mahla Shahsavar Gocmen, Ayse Dulda","doi":"10.1088/2050-6120/ad326e","DOIUrl":"10.1088/2050-6120/ad326e","url":null,"abstract":"Lanthanide-doped fluoride nanocrystals (NCs) exhibit excellent optical features, including upconversion and downconversion luminescence (UCL and DCL), that can be utilized in a variety of applications. In this study, we have successfully demonstrated the photoluminescence behavior of triple-doped NaYF4: Yb3+, Er3+, Pr3+NCs in the Vis-NIR region. Herein, highly monodisperse hexagonal phase NaYF4: Yb0.2, Er0.02, Prxnanocrystals in various Pr3+(x = 0, 0.1, 0.5, and 1 mol %) concentration with ∼22 nm diameter synthesized by thermal decomposition technique. The photoluminescence studies for all samples were performed under 980 nm laser excitation. The luminescence intensity of Er3+including blue (407 nm), green (520 and 540 nm), red (654 nm), and near-infrared (845 nm and 1530 nm) emissions was significantly quenched and Pr3+emission intensity at 1290 nm (Pr3+:1G4→3H5) changes irregularly upon doping with Pr3+ions. Furthermore, we performed the excitation power dependence and decay time analysis to investigate the energy transfer and upconversion mechanisms of samples. These findings indicate that the presence of praseodymium strongly reduces emission intensities due to abundant cross-relaxation channels. In addition, particle size is an efficient factor, shedding light on the influence of Pr3+on the energy transfer and upconversion mechanisms of the fluorides.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calixarenes and cyclodextrins as off- and on-fluorescence probes for carbazole. 作为咔唑的荧光探针和荧光探针的钙烯类和环糊精。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-03-21 DOI: 10.1088/2050-6120/ad326d

Matías E Carranza, Hugo M Elero, Pedro J Peña Hernández, Alicia V Veglia

{"title":"Calixarenes and cyclodextrins as off- and on-fluorescence probes for carbazole.","authors":"Matías E Carranza, Hugo M Elero, Pedro J Peña Hernández, Alicia V Veglia","doi":"10.1088/2050-6120/ad326d","DOIUrl":"10.1088/2050-6120/ad326d","url":null,"abstract":"Absorption and fluorescence spectra of the nitrogen polycyclic aromatic hydrocarbon carbazole (CZL) were analyzed with native cyclodextrins (CD;α,β,γ); derivatizedCD(hydroxypropyl-β-cyclodextrin,HPCD; methyl-β-cyclodextrin,MeCD) and p-sulfonated calixarenes (SCAn, with n = 6 and 8) macrocycles. The results showed a slight increase in the absorbance ofCZLwithCD, but the mixture ofCZLwithSCAshowed lower absorption than the sum of the individual spectra. Also, changes in fluorescence were observed by adding the macrocycles, quenching withSCA, and significant increases withCD. The higher fluorescence enhancement was withHPCDrationalized as a complex formation with 1:1 stoichiometry, with an average value for the association constant (KA) of (12 ± 1) x 102M-1, and a quantum yield ratio between the complexedCZLand freeCZL(ΦCZL-HPCD/ΦCZL) of (1.56 ± 0.02) at neutral pH and 25.0 °C. These increases in fluorescence were used as an on-fluorescence switch to develop a supramolecular analytical method forCZLin aqueous samples. The best analytical parameters were inHPCD(LOD = 1.41 ± 0.01 ng mL-1). The method was validated in aqueous samples of river and tap water with recoveries between 96%-104%. The proposed supramolecular method is quick, direct, selective and represents an alternative and low-cost analysis method.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140102004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving STED microscopy with SUPPOSe: enhancing resolution from a single-image. 利用 SUPPOSe 改进 STED 显微镜：提高单张图像的分辨率。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-03-19 DOI: 10.1088/2050-6120/ad31b8

Micaela Toscani, Axel M Lacapmesure

{"title":"Improving STED microscopy with SUPPOSe: enhancing resolution from a single-image.","authors":"Micaela Toscani, Axel M Lacapmesure","doi":"10.1088/2050-6120/ad31b8","DOIUrl":"10.1088/2050-6120/ad31b8","url":null,"abstract":"Here we apply the SUPPOSe algorithm on images acquired using Stimulated Emission Depletion (STED) microscopy with the aim of improving the resolution limit achieved. We processed images of the nuclear pore complex (NPC) from cell lines in which the Nup96 nucleoporin was endogenously labeled. This reference protein forms a ring whose diameter is ∼107 nm with 8 corners ∼42 nm apart from each other. The stereotypic arrangement of proteins in the NPC has been used as reference structures to characterize the performance of a variety of microscopy techniques. STED microscopy images resolve the ring arrangement but not the eightfold symmetry of the NPC. After applying the SUPPOSe algorithm to the STED images, we were able to solve the octagonal structure of the NPC. After processing 562 single NPC, the average radius of the NPC was found to beR= 54.2 ± 2.9 nm, being consistent with the theoretical distances of this structure. To verify that the solutions obtained are compatible with a NPC-type geometry, we rotate the solutions to optimally fit an eightfold-symmetric pattern and we count the number of corners that contain at least one localization. Fitting a probabilistic model to the histogram of the number of bright corners gives an effective labeling efficiency of 31%, which is in agreement with the values reported in for other cell lines and ligands used in Single Molecule Localization microscopy, showing that SUPPOSe can reliably retrieve sub-resolution, nanoscale objects from single acquisitions even in noisy conditions.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140065499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formation of gel and solid phases in acrylic cuvettes upon exposure to DMSO, oxygen and light: implications for fluorescence spectroscopy. 暴露于二甲基亚砜、氧气和光线时丙烯酸比色皿中凝胶和固相的形成：对荧光光谱学的影响

IF 2.4 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-03-15 DOI: 10.1088/2050-6120/ad2f39

Alain Bolaño Alvarez, Steffen B Petersen

引用次数: 0

Interactions between forsythoside E and two cholinesterases at the different conditions: fluorescence sections. 连翘苷 E 与两种胆碱酯酶在不同条件下的相互作用：荧光切片。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-03-12 DOI: 10.1088/2050-6120/ad2f3a

Conghuan Lin, Huizhi Du

{"title":"Interactions between forsythoside E and two cholinesterases at the different conditions: fluorescence sections.","authors":"Conghuan Lin, Huizhi Du","doi":"10.1088/2050-6120/ad2f3a","DOIUrl":"10.1088/2050-6120/ad2f3a","url":null,"abstract":"Forsythoside E is one secondary metabolite ofForsythia suspensa(Thunb.) Vahl. In the study, the interactions between forsythoside E and two types of cholinesterases, acetylcholinesterase and butyrylcholinesterase were investigated in the different conditions. Forsythoside E increased the fluorescence intensity of acetylcholinesterase but quenched the fluorescence of butyrylcholinesterase. Aβ25-35used in the study may not form complexes with cholinesterases, and did not affect the interaction between forsythoside E and cholinesterases. The charged quaternary group of AsCh interacted with the 'anionic' subsite in acetylcholinesterase, which did not affect the interaction between forsythoside E and acetylcholinesterase. The enhancement rate of forsythoside E to acetylcholinesterase fluorescence from high to low was acid solution (pH 6.4), neutral solution (pH 7.4) and alkaline solution (pH 8.0), while the reduction rate of forsythoside E to butyrylcholinesterase fluorescence was in reverse order. Metal ions may interact with cholinesterases, and increased the effects of forsythoside E to cholinesterases fluorescence, in order that Fe3+was the highest, followed by Cu2+, and Mg2+. A forsythoside E-butyrylcholinesterase complex at stoichiometric ratio of 1:1 was spontaneously formed, and the static quenching was the main quenching mode in the process of forsythoside E binding with butyrylcholinesterase. TheKvalues of two complexes were pretty much the same, suggesting that the interaction between cholinesterases and forsythoside E was almost unaffected by acid-base environment and metal ions. Thennumbers of two cholinesterases approximately equaled to one, indicating that there was only one site on each cholinesterase applicable for forsythoside E to bind to.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applications of machine learning in time-domain fluorescence lifetime imaging: a review. 机器学习在时域荧光寿命成像中的应用综述。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-02-08 DOI: 10.1088/2050-6120/ad12f7

Dorian Gouzou, Ali Taimori, Tarek Haloubi, Neil Finlayson, Qiang Wang, James R Hopgood, Marta Vallejo

{"title":"Applications of machine learning in time-domain fluorescence lifetime imaging: a review.","authors":"Dorian Gouzou, Ali Taimori, Tarek Haloubi, Neil Finlayson, Qiang Wang, James R Hopgood, Marta Vallejo","doi":"10.1088/2050-6120/ad12f7","DOIUrl":"10.1088/2050-6120/ad12f7","url":null,"abstract":"Many medical imaging modalities have benefited from recent advances in Machine Learning (ML), specifically in deep learning, such as neural networks. Computers can be trained to investigate and enhance medical imaging methods without using valuable human resources. In recent years, Fluorescence Lifetime Imaging (FLIm) has received increasing attention from the ML community. FLIm goes beyond conventional spectral imaging, providing additional lifetime information, and could lead to optical histopathology supporting real-time diagnostics. However, most current studies do not use the full potential of machine/deep learning models. As a developing image modality, FLIm data are not easily obtainable, which, coupled with an absence of standardisation, is pushing back the research to develop models which could advance automated diagnosis and help promote FLIm. In this paper, we describe recent developments that improve FLIm image quality, specifically time-domain systems, and we summarise sensing, signal-to-noise analysis and the advances in registration and low-level tracking. We review the two main applications of ML for FLIm: lifetime estimation and image analysis through classification and segmentation. We suggest a course of action to improve the quality of ML studies applied to FLIm. Our final goal is to promote FLIm and attract more ML practitioners to explore the potential of lifetime imaging.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10851337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138498873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hidden in plain sight: hyperspectral documentation of complex biofluorescence produced by the green sea urchin (Strongylocentrotus droebachiensis). 隐藏在众目睽睽之下：高光谱记录绿海胆（Strongylocentrotus droebachiensis）产生的复杂生物荧光。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-02-07 DOI: 10.1088/2050-6120/ad232e

Thomas Juhasz-Dora, Philip James, Tor Evensen, Stein-Kato Lindberg

{"title":"Hidden in plain sight: hyperspectral documentation of complex biofluorescence produced by the green sea urchin (Strongylocentrotus droebachiensis).","authors":"Thomas Juhasz-Dora, Philip James, Tor Evensen, Stein-Kato Lindberg","doi":"10.1088/2050-6120/ad232e","DOIUrl":"10.1088/2050-6120/ad232e","url":null,"abstract":"Biofluorescence in echinoderms is largely unexplored, and even though the green sea urchinStrongylocentrotus droebachiensisis a well-studied species, the presence and/or function of fluorescence remains very poorly understood. Hyperspectral imaging was conducted on adult sea urchins (N = 380) while fluorospectrometric analysis was conducted on sea urchin coelomic fluid (N = 30). Fluorescence was documented in both the spines and coelomic fluid ofS. droebachiensis. Intact spines exhibited a low intensity green emission (∼550-600 nm), while broken spines averaged a high emission peak in the green spectrum (∼580 nm). Sea urchins produce a red exudate with a pronounced emission peak (∼680 nm) with a shoulder peak (∼730 nm). The sampled coelomic fluid exhibited high variability, with a majority exhibiting a low-level green fluorescence while pronounced emission peaks (N = 5) were found in the red spectrum (∼680 nm). The complex fluorescence produced byS. droebachiensiswarrants further investigation on its applicability for monitoring welfare of sea urchins in aquaculture facilities.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139567266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3-aminophenylboronic acid modified carbon nitride quantum dots as fluorescent probe for selective detection of dopamine and cell imaging. 3-aminophenylboronic Acid Modified Carbon Nitride Quantum Dots as Fluorescent Probe for Selective Detection of Dopamine and Cell Imaging.

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2024-01-03 DOI: 10.1088/2050-6120/ad17a1

Mingyu Gan, Rui Yao, Baoping Wang, Jiarong Li, Ning Wang, Martin M F Choi, Wei Bian

{"title":"3-aminophenylboronic acid modified carbon nitride quantum dots as fluorescent probe for selective detection of dopamine and cell imaging.","authors":"Mingyu Gan, Rui Yao, Baoping Wang, Jiarong Li, Ning Wang, Martin M F Choi, Wei Bian","doi":"10.1088/2050-6120/ad17a1","DOIUrl":"10.1088/2050-6120/ad17a1","url":null,"abstract":"Dopamine (DA) is the most abundant catecholamine neurotransmitter in the brain and plays an extremely essential role in the physiological activities of the living organism. There is a critical need for accurately and efficiently detecting DA levels in organisms in order to reflect physiological states. Carbon nitride quantum dots (C3N4) were, in recent years, used enormously as electrochemical and fluorescence probes for the detection of metal ions, biomarkers and other environmental or food impurities due to their unique advantageous optical and electronic properties. 3-Aminophenylboronic acid (3-APBA) can specifically combine with DA through an aggregation effect, providing an effective DA detection method. In this work, 3-APBA modified carbon nitride quantum dots (3-APBA-CNQDs) were synthesized from urea and sodium citrate. The structure, chemical composition and optical properties of 3-APBA-CNQDs were investigated by XRD, TEM, UV-visible, and FT-IR spectroscopy. The addition of DA could induce fluorescence quenching of 3-APBA-CNQDs possibly through the inner filter effect (IFE). 3-APBA-CNQDs shows better selectivity and sensitivity to DA than other interfering substances. By optimizing the experiment conditions, good linearity was obtained at 0.10-51μM DA with a low detection limit of 22.08 nM. More importantly, 3-APBA-CNQDs have been successfully applied for the detection of DA in human urine and blood samples as well as for bioimaging of intracellular DA. This study provides a promising novel method for the rapid detection of DA in real biological samples.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0