用于减少正面荧光光谱散射的简单光路修正装置。

IF 2.4 3区化学 Q3 CHEMISTRY, ANALYTICAL

Methods and Applications in Fluorescence Pub Date : 2024-06-17 DOI:10.1088/2050-6120/ad5415

Frank B Peters, Andreas O Rapp

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引用次数: 0

摘要

本技术说明介绍了一种在获得荧光信号的同时减少正面荧光光谱中散射信号的装置。商用荧光光谱仪的光束路径由两面偏转镜改变，使反射远离传感器。用两种流体和三种固体物质对这种光路修正（LPM）装置进行了测试，散射与荧光比提高了 1.7 到 7.6 倍。使用 LPM 获得的光谱更加清晰，避免了荧光峰的扭曲。硫酸奎宁的扫描结果与参考光谱十分吻合。

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A simple light path modifying device to reduce scattering in front-face fluorescence spectra.

This technical note presents a device to diminish scattering signal in front-face fluorescence spectra while obtaining fluorescence signal. The beam path in a commercial fluorescence spectrometer was modified by two deflecting mirrors, leading reflections away from the sensor. This light path modifying (LPM) device was tested with two fluid and three solid substances, where the scattering-to-fluorescence ratio improved by a factor of 1.7 to 7.6. The spectra obtained with the LPM were much clearer, and distortion of the fluorescence peaks was avoided. Scans of quinine sulphate complied well with reference spectra.

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来源期刊

Methods and Applications in Fluorescence CHEMISTRY, ANALYTICALCHEMISTRY, PHYSICAL&n-CHEMISTRY, PHYSICAL

CiteScore

6.20

自引率

3.10%

发文量

期刊介绍： Methods and Applications in Fluorescence focuses on new developments in fluorescence spectroscopy, imaging, microscopy, fluorescent probes, labels and (nano)materials. It will feature both methods and advanced (bio)applications and accepts original research articles, reviews and technical notes.