Methods and Applications in Fluorescence最新文献_第7页

Surface plasmon enhanced fluorescence: self-consistent classical treatment in the quasi-static limit. 表面等离子体增强荧光:准静态极限下的自洽经典处理。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-04-20 DOI: 10.1088/2050-6120/acca62

Dentcho A Genov

{"title":"Surface plasmon enhanced fluorescence: self-consistent classical treatment in the quasi-static limit.","authors":"Dentcho A Genov","doi":"10.1088/2050-6120/acca62","DOIUrl":"https://doi.org/10.1088/2050-6120/acca62","url":null,"abstract":"The problem of enhanced molecular emission in close proximity to dielectric and metallic interfaces is of great importance for many physical and biological applications. Here we present an exact treatment of the problem from the view point of classical electromagnetism. Self-consistent analytical theory of the surface enhanced fluorescence (SEF) is developed for configurations consisting of an emitter in proximity to core-shell metal-dielectric nanoparticles. The dependence of the fluorescence enhancement on the excitation laser and fluorescence frequencies and distance of the emitter to the nanoparticle interface are studied. The developed theory predicts enhanced fluorescence at intermediate distances as well as emission quenching into non-radiative surface plasmon (SP) modes dominating the response for short distances. The conditions for optimal emission enhancement for two core-shell configurations are determined and a comparison to published experimental data is performed showing a good correspondence between theory and experiment. The developed model can be applied toward analyzes and optimizations of various applications related to SP enhance fluorescence spectroscopy.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9325749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual emission and itsλ-ratiometric detection in analytical fluorimetry Pt. II. Exploration in sensing and imaging. 分析荧光法中的双发射及其λ-比率检测[j]。传感与成像探索。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-04-18 DOI: 10.1088/2050-6120/acc715

Alexander P Demchenko

{"title":"Dual emission and itsλ-ratiometric detection in analytical fluorimetry Pt. II. Exploration in sensing and imaging.","authors":"Alexander P Demchenko","doi":"10.1088/2050-6120/acc715","DOIUrl":"https://doi.org/10.1088/2050-6120/acc715","url":null,"abstract":"The wavelength-ratiometric techniques demonstrate strong advantages in fluorescence sensing and imaging over techniques employing variations of intensity at single wavelength. We present different possibilities for realization of these advantages in different simplified, miniaturized and multiplexing devices. They include the smartphone-based detection systems and strips, in which the color changes are observed with naked eye. The array-based techniques and different immunoassays withλ-ratiometric detection demonstrate strongly increased stability and sensitivity. The application areas extend from on-site monitoring of environment and point-of-care diagnostics to testing in personal need. Selected examples of sensing different analytes in chemical and biological systems demonstrate multiple possibilities of coupling the analyte-sensor interaction with the generation ofλ-ratiometric output signal. Among them, simultaneous detection of several analytes and performing logical operations that can be useful in analysis. Finally, the benefits of multicolor ratiometric fluorescence imaging are demonstrated by visualization the functionally important parameters of biological membranes.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9377952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Corrigendum: Pulse-shaped broadband multiphoton excitation for single-molecule fluorescence detection in the far field (2023Methods Appl. Fluoresc.11017001). 勘误:用于远场单分子荧光检测的脉冲形宽带多光子激发(2023)Fluoresc.11017001)。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-04-14 DOI: 10.1088/2050-6120/acca63

David Nobis, Henry G Sansom, Steven W Magennis

引用次数: 0

Fluorescence quenching determination of tetracyclines based on the synergistic oxidation effect between Fe₃O₄nanoparticles and tetracyclines. 基于fe3o4纳米颗粒与四环素协同氧化效应的荧光猝灭测定四环素。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-04-13 DOI: 10.1088/2050-6120/acca61

Shiqi Guo, Yilin Chai, Yongjun Wu, Hongchao Guo, Fei Yu, Li-E Liu, Leiliang He, Songcheng Yu, Yongmei Tian, Jia Wang, Ruiying Yang, Ningge Jian, Yilin Wang

{"title":"Fluorescence quenching determination of tetracyclines based on the synergistic oxidation effect between Fe3O4nanoparticles and tetracyclines.","authors":"Shiqi Guo, Yilin Chai, Yongjun Wu, Hongchao Guo, Fei Yu, Li-E Liu, Leiliang He, Songcheng Yu, Yongmei Tian, Jia Wang, Ruiying Yang, Ningge Jian, Yilin Wang","doi":"10.1088/2050-6120/acca61","DOIUrl":"https://doi.org/10.1088/2050-6120/acca61","url":null,"abstract":"In recent years, tetracyclines (TCs) is a hot research topic. Herein, we report an interesting discovery using the complexation of oxytetracycline and metal ions. In this study, according to the properties of Fe3O4nanoparticles (Fe3O4NPs) as a nanoenzyme, it can be used to catalyze the oxidation of KI by H2O2to produceI3-,while at the same timeI3-binds to rhodamine 6G (Rh6G) to form a conjoined particle (Rh6G ∼ I3)n, leading to a decrease in the fluorescence intensity of Rh6G. However, in the presence of TCs, Fe3O4NPs have a synergistic effect with TCs, leading to enhanced catalytic activity, as well as better selectivity compared to the activity of other reducing enzymes. Consequently,the fluorescent signal based on a resonance scattering effect between Rh6G andI3-is dependent on the concentration of TCs, thus achieving highly facile and robust detection of TCs. The limits of detection (LOD) of the method were 20 nM, 10 nM and 40 nM for oxytetracycline(OTC), tetracycline(TC) and chlortetracycline(CTC), respectively. Most importantly, the method can be successfully applied to the detection of TCs in milk, eggs, and honey. The recoveries of spiked samples ranged from 83.11 to 118.95%. Thus, a stable, hands-on strategy for the detection of TCs is proposed, which has potential applications in the field of food safety and environmental protection.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 3","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9316156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Room Temperature Phosphorescence of 5,6-Benzoquinoline. 5,6-苯并喹啉的室温磷光。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-04-03 DOI: 10.1088/2050-6120/acc716

Jose Chavez, Luca Ceresa, Emma Kitchner, Danh Pham, Zygmunt Gryczynski, Ignacy Gryczynski

{"title":"Room Temperature Phosphorescence of 5,6-Benzoquinoline.","authors":"Jose Chavez, Luca Ceresa, Emma Kitchner, Danh Pham, Zygmunt Gryczynski, Ignacy Gryczynski","doi":"10.1088/2050-6120/acc716","DOIUrl":"https://doi.org/10.1088/2050-6120/acc716","url":null,"abstract":"Phosphorescence emission of 5,6-Benzoquinoline embedded in poly (vinyl alcohol) film has been studied at room temperature. A strong green long-lived emission was observed in films doped with 5,6-Benzoquinoline while illuminated on a UV plate. A broad phosphorescence emission spectrum is centered at about 500 nm. The phosphorescence excitation spectrum follows the absorption spectrum of 5,6-Benzoquinoline, except for a long-wavelength part, which is well beyond the absorption band. This long-wavelength part of the absorption spectrum is responsible for the forbidden S0-T1transition. The excitation at 430 nm resulted in the long-lived emission with a spectrum similar to the phosphorescence spectrum obtained with UV excitation within the absorption of 5,6-Benzoquinoline. The phosphorescence anisotropy obtained with a direct S0-T1excitation is positive, while the UV excitation is negative. In contrast to fluorescence, the phosphorescence intensity strongly depends on temperature. Phosphorescence lifetimes with UV and long-wavelength excitation are similar, with a mean value of about 0.5 s.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9587122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescent carbon dots tailored iron oxide nano hybrid system forin vivooptical imaging of liver fibrosis. 荧光碳点定制氧化铁纳米混合系统用于肝纤维化的体内光学成像。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-03-21 DOI: 10.1088/2050-6120/acc009

Shaiju S Nazeer, Ariya Saraswathy, Nirmala Nimi, Elangovan Sarathkumar, A N Resmi, Sachin J Shenoy, Ramapurath S Jayasree

{"title":"Fluorescent carbon dots tailored iron oxide nano hybrid system forin vivooptical imaging of liver fibrosis.","authors":"Shaiju S Nazeer, Ariya Saraswathy, Nirmala Nimi, Elangovan Sarathkumar, A N Resmi, Sachin J Shenoy, Ramapurath S Jayasree","doi":"10.1088/2050-6120/acc009","DOIUrl":"https://doi.org/10.1088/2050-6120/acc009","url":null,"abstract":"Hybrid nanoparticles are innovative invention of last decade designed to overcome limitations of single-component nanoparticles by introducing multiple functionalities through combining two or more different nanoparticles. In this study, we are reporting development of magneto-fluorescent hybrid nanoparticles by combining iron oxide and carbon nanoparticles to enablein vivofluorescence imaging which also has all the required characteristic properties to use as Magnetic Resonance Imaging (MRI) contrast agent. In order to achieve dual-functional imaging, alginate and pullulan coated super paramagnetic iron oxide nanoparticles (ASPION and PSPION) and Carbon dots (Cdts) were synthesised separately. ASPIONs and PSPIONs were further chemically conjugated with Cdts and developed dual-functional nanohybrid particles ASPION-Cdts and PSPION-Cdts. Subsequently, evaluation of the materials for its size, functionalisation efficiency, fluorescence and magnetic properties, biocompatibility and cellular uptake efficiency has been carried out. Fluorescence imaging of liver fibrosis was performedin vivoin rodent model of liver fibrosis using the two nanohybrids, which is further confirmed by high fluorescence signal from the harvested liver.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10311824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Compact and robust deep learning architecture for fluorescence lifetime imaging and FPGA implementation. 用于荧光寿命成像和FPGA实现的紧凑和健壮的深度学习架构。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-03-20 DOI: 10.1088/2050-6120/acc0d9

Zhenya Zang, Dong Xiao, Quan Wang, Ziao Jiao, Yu Chen, David Day-Uei Li

{"title":"Compact and robust deep learning architecture for fluorescence lifetime imaging and FPGA implementation.","authors":"Zhenya Zang, Dong Xiao, Quan Wang, Ziao Jiao, Yu Chen, David Day-Uei Li","doi":"10.1088/2050-6120/acc0d9","DOIUrl":"https://doi.org/10.1088/2050-6120/acc0d9","url":null,"abstract":"This paper reports a bespoke adder-based deep learning network for time-domain fluorescence lifetime imaging (FLIM). By leveraging thel1-norm extraction method, we propose a 1D Fluorescence Lifetime AdderNet (FLAN) without multiplication-based convolutions to reduce the computational complexity. Further, we compressed fluorescence decays in temporal dimension using a log-scale merging technique to discard redundant temporal information derived as log-scaling FLAN (FLAN+LS). FLAN+LS achieves 0.11 and 0.23 compression ratios compared with FLAN and a conventional 1D convolutional neural network (1D CNN) while maintaining high accuracy in retrieving lifetimes. We extensively evaluated FLAN and FLAN+LS using synthetic and real data. A traditional fitting method and other non-fitting, high-accuracy algorithms were compared with our networks for synthetic data. Our networks attained a minor reconstruction error in different photon-count scenarios. For real data, we used fluorescent beads' data acquired by a confocal microscope to validate the effectiveness of real fluorophores, and our networks can differentiate beads with different lifetimes. Additionally, we implemented the network architecture on a field-programmable gate array (FPGA) with a post-quantization technique to shorten the bit-width, thereby improving computing efficiency. FLAN+LS on hardware achieves the highest computing efficiency compared to 1D CNN and FLAN. We also discussed the applicability of our network and hardware architecture for other time-resolved biomedical applications using photon-efficient, time-resolved sensors.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9139171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Multi-stimuli-responsive and dynamic color tunable security ink for multilevel anticounterfeiting. 多刺激响应和动态颜色可调安全油墨，多级防伪。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-03-15 DOI: 10.1088/2050-6120/acbe92

Charu Dubey, Anjana Yadav, Diksha Baloni, Sachin Singh, Anjani Kumar Singh, Sunil Kumar Singh, Akhilesh Kumar Singh

{"title":"Multi-stimuli-responsive and dynamic color tunable security ink for multilevel anticounterfeiting.","authors":"Charu Dubey, Anjana Yadav, Diksha Baloni, Sachin Singh, Anjani Kumar Singh, Sunil Kumar Singh, Akhilesh Kumar Singh","doi":"10.1088/2050-6120/acbe92","DOIUrl":"https://doi.org/10.1088/2050-6120/acbe92","url":null,"abstract":"Luminescent security features have been used for anticounterfeiting for a long time. However, constant effort is required to strengthen these security features to be ahead of counterfeiters. Here, we developed a multi-stimuli-responsive luminescent security ink containing Tb(ASA)3Phen, K2SiF6:Mn4+,and NaYF4:Yb3+/Er3+luminescent materials in PVC gold medium. Tb(ASA)3Phen complex shows a broad excitation band in the UV region; upon UV light radiation it shows strong greenish emission of Tb3+ions through the antenna effect. K2SiF6:Mn4+, on the other hand, has three excitation bands with maxima at 248, 354, and 454 nm which emit red light after excitation through these bands. NaYF4:Yb3+/Er3+is used as an upconverting nanophosphor showing green emission under 976 nm laser excitation. Thus, the multi-stimuli-responsive luminescent security ink shows greenish, red, and green emissions under 367 nm, 450 nm, and 976 nm excitations, respectively. Furthermore, the distinct lifetimes of the activators in Tb(ASA)3Phen and K2SiF6:Mn4+, i.e. 0.1708 ms and 8.165 ms, respectively, under 380 nm excitation make this ink suitable for dynamic anticounterfeiting as well. The ink shows a change in the emission color with time delay, after the removal of the 380 nm excitation source, from greenish yellow (at 0 delays) to reddish color after a delay of 7.5 ms. These unique optical features along with excellent photo-, chemical- and environmental stability make this ink useful for advanced-level anticounterfeiting.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9118341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tutorial: multiphoton microscopy to advance neuroscience research. 教程:多光子显微镜推进神经科学研究。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-02-22 DOI: 10.1088/2050-6120/acba66

Iván Coto Hernández, Jenny Yau, Lars Rishøj, Nanke Cui, Steven Minderler, Nate Jowett

引用次数: 0

Enhanced emission intensity in (Li⁺/Ca²⁺/Bi³⁺) ions co-doped NaLa(MoO₄)₂: Dy³⁺phosphors and their Judd-Ofelt analysis for WLEDs applications. 增强(Li+/Ca2+/Bi3+)离子共掺杂的 NaLa(MoO4)2：Dy3+ 磷光体及其用于 WLED 应用的 Judd-Ofelt 分析。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-02-13 DOI: 10.1088/2050-6120/acbbb9

Sonali Sonali, C Shivakumara

{"title":"Enhanced emission intensity in (Li+/Ca2+/Bi3+) ions co-doped NaLa(MoO4)2: Dy3+phosphors and their Judd-Ofelt analysis for WLEDs applications.","authors":"Sonali Sonali, C Shivakumara","doi":"10.1088/2050-6120/acbbb9","DOIUrl":"10.1088/2050-6120/acbbb9","url":null,"abstract":"In the present study, we have synthesized a series of Dy3+ ion doped NaLa(MoO4)2phosphors by the conventional solid-state method at 750 °C for 4h. All the compounds were crystallized in the tetragonal scheelite type structure with space group (I41/a, No.88). The morphology and functional group were confirmed by the field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared(FTIR)spectroscopy. Upon near-Ultraviolet (n-UV) excitation, the PL spectra exhibit the two characteristic emissions of Dy3+ ions, blue (4F9/2→6H15/2) at 487 nm and yellow (4F9/2→6H13/2) at 574nm respectively. The optimum concentration of Dy3+ionis 3 mol% and then quenching occurred due to multipolar interaction. Further, enhanced the emission intensity by co-doping with monovalent (Li+), divalent (Ca2+) and trivalent (Bi3+) ions. Among them, Li+ ion co-doped samples are shown maximum intensity (50 times) more than Dy3+ doped phosphors as relaxation of parity restriction of electric dipole transition because of local distortion of crystal field around the Dy3+ ions. In addition, by incorporation of Eu3+ ions into NaLa(MoO4)2:Dy3+system, tuned the emission color from white to red, owing to energy transfer from Dy3+ to Eu3+ ions. The intensity parameters (Ω2, Ω4) and radiative properties such as transition probabilities (AT), radiative lifetime (rad), and branching ratio were calculated using the Judd-Ofelt theory. CIE color coordinates, CCT values indicates that these phosphors exhibit an excellent white emission. The determined radiative properties, CIE and CCT results revealed that the Dy3+-activated NaLa(MoO4)2phosphors are potential materials for developing white LEDs, and optoelectronic device fabrications.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9260665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0