Study of the senescence of rice leaves through stationary and time-resolved photoluminescence.

IF 2.4 3区 化学 Q3 CHEMISTRY, ANALYTICAL
Gerardo Fonthal, Liliana Tirado-Mejía, Luz Angela Giraldo-Pinto
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引用次数: 0

Abstract

This work describes the relationship between the complex of photosystem I and photosystem II in the senescence process of rice leaves observed through changes in the optical response. We studied three varieties of rice plants at different aging times using time-resolved photoluminescence to measure the time decay of the emission, and stationary photoluminescence, to measure the emission wavelength. The spectra obtained with the former technique were fitted with decreasing exponential functions. Two relaxation times were obtained, one ranging between 1.0 and 1.7 ns, and the other, from 5.0 to 10.5 ns. They are associated with the electron's deexcitation of PSI and PSII, respectively, and these decay times increase as the leaf senescence process takes place. The spectra obtained with stationary photoluminescence were fitted with Voigt functions. These spectra exhibit two main peaks around 683 and 730 nm, which could be associated mainly with PSII and PSI emissions, respectively. The PSI de-excitation exhibits higher dispersive processes because chlorophyll-a molecules in it move away from each other, decreasing their concentration. Therefore, it takes longer for electrons to recombine during photosynthesis, as seen in the time-resolve response. Articulating the results of both photoluminescence techniques, the changes in the response of the photosystems of the living rice leaves during senescence are evidenced.

用固定光致发光和时间分辨光致发光研究水稻叶片衰老。
本文通过观察光响应的变化,描述了水稻叶片衰老过程中光系统I和光系统II复合体之间的关系。利用时间分辨光致发光技术测量了三种不同老化时间的水稻植株的发射时间衰减,并利用稳态光致发光技术测量了发射波长。用前一种方法得到的光谱用递减指数函数拟合。得到两个弛豫时间,一个在1.0 ~ 1.7 ns之间,另一个在5.0 ~ 10.5 ns之间。它们分别与PSI和PSII的电子去激发有关,并且随着叶片衰老过程的发生,这些衰变时间增加。用固定光致发光得到的光谱用Voigt函数拟合。这些光谱在683 nm和730 nm附近有两个主峰,分别与PSII和PSI发射有关。PSI去激发表现出更高的色散过程,因为其中的叶绿素-a分子相互远离,降低了它们的浓度。因此,在光合作用过程中,电子重组需要更长的时间,正如时间分辨响应所示。结合两种光致发光技术的结果,证明了水稻叶片在衰老过程中光系统响应的变化。
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来源期刊
Methods and Applications in Fluorescence
Methods and Applications in Fluorescence CHEMISTRY, ANALYTICALCHEMISTRY, PHYSICAL&n-CHEMISTRY, PHYSICAL
CiteScore
6.20
自引率
3.10%
发文量
60
期刊介绍: Methods and Applications in Fluorescence focuses on new developments in fluorescence spectroscopy, imaging, microscopy, fluorescent probes, labels and (nano)materials. It will feature both methods and advanced (bio)applications and accepts original research articles, reviews and technical notes.
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