Methods and Applications in Fluorescence最新文献_第8页

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-01-03 DOI: 10.1088/2050-6120/aca5f6

David J S Birch, Marcia Levitus, Yves Mély

引用次数: 0

MINFLUX imaging of a bacterial molecular machine at nanometer resolution. 细菌分子机器的纳米分辨率MINFLUX成像。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-12-13 DOI: 10.1088/2050-6120/aca880

Alexander Carsten, Maren Rudolph, Tobias Weihs, Roman Schmidt, Isabelle Jansen, Christian A Wurm, Andreas Diepold, Antonio Virgilio Failla, Manuel Wolters, Martin Aepfelbacher

{"title":"MINFLUX imaging of a bacterial molecular machine at nanometer resolution.","authors":"Alexander Carsten, Maren Rudolph, Tobias Weihs, Roman Schmidt, Isabelle Jansen, Christian A Wurm, Andreas Diepold, Antonio Virgilio Failla, Manuel Wolters, Martin Aepfelbacher","doi":"10.1088/2050-6120/aca880","DOIUrl":"https://doi.org/10.1088/2050-6120/aca880","url":null,"abstract":"The resolution achievable with the established super-resolution fluorescence nanoscopy methods, such as STORM or STED, is in general not sufficient to resolve protein complexes or even individual proteins. Recently, minimal photon flux (MINFLUX) nanoscopy has been introduced that combines the strengths of STED and STORM nanoscopy and can achieve a localization precision of less than 5 nm. We established a generally applicable workflow for MINFLUX imaging and applied it for the first time to a bacterial molecular machinein situ, i.e., the injectisome of the enteropathogenY. enterocolitica. We demonstrate with a pore protein of the injectisome that MINFLUX can achieve a resolution down to the single molecule levelin situ. By imaging a sorting platform protein using 3D-MINFLUX, insights into the precise localization and distribution of an injectisome component in a bacterial cell could be accomplished. MINFLUX nanoscopy has the potential to revolutionize super-resolution imaging of dynamic molecular processes in bacteria and eukaryotes.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10406921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation. 角蛋白固有荧光作为一种无创监测其糖化的机制。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-12-01 DOI: 10.1088/2050-6120/aca507

Rhona Muir, Shareen Forbes, David J S Birch, Vladislav Vyshemirsky, Olaf J Rolinski

引用次数: 0

Non-invasive assessment of intestinal permeability in healthy volunteers using transcutaneous fluorescence spectroscopy. 利用经皮荧光光谱法无创评估健康志愿者肠道通透性。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-10-10 DOI: 10.1088/2050-6120/ac9513

Jonathan Gan, Elena Monfort Sánchez, James Avery, Omar Barbouti, Jonathan Hoare, Hutan Ashrafian, Ara Darzi, Alex J Thompson

{"title":"Non-invasive assessment of intestinal permeability in healthy volunteers using transcutaneous fluorescence spectroscopy.","authors":"Jonathan Gan, Elena Monfort Sánchez, James Avery, Omar Barbouti, Jonathan Hoare, Hutan Ashrafian, Ara Darzi, Alex J Thompson","doi":"10.1088/2050-6120/ac9513","DOIUrl":"https://doi.org/10.1088/2050-6120/ac9513","url":null,"abstract":"The permeability of the intestinal barrier is altered in a multitude of gastrointestinal conditions such as Crohn's and coeliac disease. However, the clinical utility of gut permeability is currently limited due to a lack of reliable diagnostic tests. To address this issue, we report a novel technique for rapid, non-invasive measurement of gut permeability based on transcutaneous ('through-the-skin') fluorescence spectroscopy. In this approach, participants drink an oral dose of a fluorescent dye (fluorescein) and a fibre-optic fluorescence spectrometer is attached to the finger to detect permeation of the dye from the gut into the blood stream in a non-invasive manner. To validate this technique, clinical trial measurements were performed in 11 healthy participants. First, after 6 h of fasting, participants ingested 500 mg of fluorescein dissolved in 100 ml of water and fluorescence measurements were recorded at the fingertip over the following 3 h. All participants were invited back for a repeat study, this time ingesting the same solution but with 60 g of sugar added (known to transiently increase intestinal permeability). Results from the two study datasets (without and with sugar respectively) were analysed and compared using a number of analysis procedures. This included both manual and automated calculation of a series of parameters designed for assessment of gut permeability. Calculated values were compared using Student's T-tests, which demonstrated significant differences between the two datasets. Thus, transcutaneous fluorescence spectroscopy shows promise in non-invasively discriminating between two differing states of gut permeability, demonstrating potential for future clinical use.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"10 4","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10590297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Photoluminescence behavior of Eu3+ doped XAl2O4 (X = Mg, Ca, Sr and Ba) phosphors: a comparative study Eu3+掺杂XAl2O4 (X = Mg, Ca, Sr和Ba)荧光粉的光致发光行为比较研究

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-06-15 DOI: 10.1088/2050-6120/ac7942

A. Choudhary, A. Dwivedi, S. Rai

{"title":"Photoluminescence behavior of Eu3+ doped XAl2O4 (X = Mg, Ca, Sr and Ba) phosphors: a comparative study","authors":"A. Choudhary, A. Dwivedi, S. Rai","doi":"10.1088/2050-6120/ac7942","DOIUrl":"https://doi.org/10.1088/2050-6120/ac7942","url":null,"abstract":"In this work, the Eu3+ doped stuffed tridymite type structure of alkaline earths aluminate i.e. XAl2O4 (X = Mg, Ca, Sr and Ba) phosphor materials have been synthesized by conventional high temperature solid state reaction method at 1623 K. The Samples were structurally and morphologically characterized by x-ray diffraction (XRD) and Scanning electron microscope (SEM) measurements. The vibrational behavior of the phosphor samples were investigated by Fourier transform infrared (FTIR) measurements. The phosphor samples emit intense red emission in 610–615 nm range due to 5D0 → 7F2 transition of Eu3+ ion on excitation with charge transfer band (CTB) wavelength arising due to Eu3+-O2- and also by the discrete bands of Eu3+ ions .The decay time of 5D0 level of Eu3+ ion were recorded on excitation with 393 nm and by the CTB wavelength for all the four samples. The optimized 1 mol% Eu3+ doped CaAl2O4 phosphor exhibits optimum emission intensity and color purity under the excitation with 393 nm than others. The decay time is also found to be larger in the case of Eu3+ doped CaAl2O4 phosphor sample. Therefore, Eu3+ doped CaAl2O4 phosphor may be promising material for red color light emitting applications and white light generation.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47643624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Red fluorescent BODIPY molecular rotor for high microviscosity environments 用于高微粘度环境的红色荧光BODIPY分子转子

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-06-15 DOI: 10.1088/2050-6120/ac7943

Karolina Maleckaitė, Jelena Dodonova-Vaitkūnienė, Rugilė Žilėnaitė, S. Tumkevičius, Aurimas Vyšniauskas

{"title":"Red fluorescent BODIPY molecular rotor for high microviscosity environments","authors":"Karolina Maleckaitė, Jelena Dodonova-Vaitkūnienė, Rugilė Žilėnaitė, S. Tumkevičius, Aurimas Vyšniauskas","doi":"10.1088/2050-6120/ac7943","DOIUrl":"https://doi.org/10.1088/2050-6120/ac7943","url":null,"abstract":"Microviscosity has a strong impact for diffusion-controlled processes in biological environments. BODIPY molecular rotors are viscosity-sensitive fluorophores that provide a simple and non-invasive way to visualise microviscosity. Although green fluorescent probes are already well developed for imaging, thick biological samples require longer wavelengths for investigation. This work focuses on the examination of novel β-substituted meso-phenyl-BODIPYs possessing a red emission. We report a new red fluorescent BODIPY-based probe BP-Vinyl-NO2 suitable for sensing microviscosity in rigid environments of over 100 000 cP viscosities. Furthermore, we demonstrate that changing the methyl position from ortho to meta on the β-phenyl-substituted conjugate BP-PH-m2M-NO2 redshifts absorbance and fluorescence spectra while maintaining viscosity sensitivity. Finally, we show that nitro-substitution of meso-phenyl is a versatile approach to improve the sensitivity to viscosity while suppressing sensitivity to polarity and temperature of such derivatives. In summary, we present two nitro-substituted red fluorescent probes that could be used as lifetime-based microviscosity sensors.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43344188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile synthesis of sulfur and oxygen co-doped graphitic carbon nitride quantum dots for on-off detection of Cu2+ in real samples and living cells 硫氧共掺杂石墨氮化碳量子点的简单合成，用于实际样品和活细胞中Cu2+的开关检测

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-06-15 DOI: 10.1088/2050-6120/ac7944

Mengting Zhang, Yulu Zhang, Mingyu Gan, Liping Xie, Jing Wang, Wei‐Hua Jia, W. Bian, S. Shuang, Martin M. F. Choi

{"title":"Facile synthesis of sulfur and oxygen co-doped graphitic carbon nitride quantum dots for on-off detection of Cu2+ in real samples and living cells","authors":"Mengting Zhang, Yulu Zhang, Mingyu Gan, Liping Xie, Jing Wang, Wei‐Hua Jia, W. Bian, S. Shuang, Martin M. F. Choi","doi":"10.1088/2050-6120/ac7944","DOIUrl":"https://doi.org/10.1088/2050-6120/ac7944","url":null,"abstract":"A fluorescent sulfur and oxygen co-doped graphitic carbon nitride quantum dots (S,O-CNQDs) were prepared from ethylenediaminetetraacetic acid disodium salt dihydrate and thiourea as the carbon and sulfur sources. The morphology and surface functional groups of S,O-CNQDs were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The fluorescence of S,O-CNQDs could be quenched efficiently by Cu2+ under the optimum conditions. The S,O-CNQDs could function as an excellent fluorescent probe for Cu2+ detection with a wide linear range of 0.50–15 μM and a low detection limit of 0.58 nM. In addition, this fluorescent probe was employed for monitoring Cu2+ in samples of tap water, lake water, human serum and urine with good recoveries from 99.0% to 110.0%. Moreover, the S,O-CNQDs with high cell penetration and low cytotoxicity were utilized for Cu2+ detection in living cells. Owing to the excellent properties of S,O-CNQDs, the as-prepared S,O-CNQDs can be a potential candidate for biological applications.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45328556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

3-aminoquinoline: a turn-on fluorescent probe for preferential solvation in binary solvent mixtures 3-氨基喹啉：一种用于二元溶剂混合物中优先溶剂化的开启荧光探针

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-06-13 DOI: 10.1088/2050-6120/ac784d

Sharmistha Das, Shirsendu Das, A. Singh, A. Datta

{"title":"3-aminoquinoline: a turn-on fluorescent probe for preferential solvation in binary solvent mixtures","authors":"Sharmistha Das, Shirsendu Das, A. Singh, A. Datta","doi":"10.1088/2050-6120/ac784d","DOIUrl":"https://doi.org/10.1088/2050-6120/ac784d","url":null,"abstract":"3-Aminoquinoline (3AQ) has been used as a fluorescent probe for preferential solvation in hexane-ethanol solvent mixtures. Results of the present experiment have been put into context by comparison with prior observations with 5-aminoquinoline (5AQ) as the probe. 3AQ exhibits a relatively small change of dipole moment (Δμ = 2.2 D) upon photoexcitation, compared to 5AQ (Δμ = 6.1D), which might appear to be a hindrance in the way of its use as a solvation probe. Indeed, the values of parameters like spectral shifts are smaller for the present experiment with 3AQ. At the smallest concentration of alcohol used, its local mole fraction around the probe is significantly lower than in the previous experiments with 5AQ. However, these apparent disadvantages are outweighed by the significant increase in fluorescence intensity and lifetime observed with increasing concentration of ethanol in the solvent mixture, as opposed to the drastic fluorescence quenching that occurs for 5AQ. This is a marked advantage in the use of 3AQ in studies like the present one. The local mole fraction of ethanol and preferential solvation index experienced by 3AQ are in line with those reported for 5AQ. The disadvantage of the smaller magnitude of Δμ persists in the time resolved fluorescence experiments, for solvent mixtures with very low ethanol content. Negligible wavelength dependence of fluorescence transients of 3AQ is observed for x p = 0.002,. However, this effect is outweighed at higher alcohol concentrations, for which nanosecond dynamics of preferential solvation is observed.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44887679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two near-infrared fluorescent probes based on dicyanoisfluorone for rapid monitoring of Zn2+ and Pb2+ 基于二氰芴酮的两种近红外荧光探针快速监测Zn2+和Pb2+

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-05-19 DOI: 10.1088/2050-6120/ac7199

Jia Li, Cuiping Zhou, Hong Yang, Xiongzhi Wu, Liqiang Yan

{"title":"Two near-infrared fluorescent probes based on dicyanoisfluorone for rapid monitoring of Zn2+ and Pb2+","authors":"Jia Li, Cuiping Zhou, Hong Yang, Xiongzhi Wu, Liqiang Yan","doi":"10.1088/2050-6120/ac7199","DOIUrl":"https://doi.org/10.1088/2050-6120/ac7199","url":null,"abstract":"Zinc (Zn2+) and lead (Pb2+) ions in the environment have important effects on human health and environmental safety. Therefore, it is necessary to effectively detect them by a convenient and reliable analysis method. In this study, two near-infrared fluorescent probes for the fast determination of Zn2+ and Pb2+ were synthesized by a simple Schiff base reaction between the dicyanoisophorone skeleton and carbohydrazide derivatives. Among them, the probe with the thiophene-2-carbohydrazide group showed a selective fluorescence response to Zn2+ and Pb2+ with a maximum emission wavelength of 670 nm. And the detection limits of the probe for Zn2+ and Pb2+ were 1.59 nM and 1.65 nM, respectively. In contrast the probe modified by the furan-2-carbohydrazide group achieved quantitative detection of Zn2+, with a detection limit of 2.7 nM. These results were attributed to the fact that the probes bind to Zn2+ and Pb2+ in stoichiometric ratios of 1:1, blocking the intramolecular PET effect. Furthermore, these two probes can be recycled through the action of EDTA and have been successfully used to detect Zn2+ and Pb2+ in real water samples.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46908505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A novel reversible fluorescent probe for Cu2+ and S2− ions and imaging in living cells 一种新型可逆的Cu2+和S2−离子荧光探针及其在活细胞中的成像

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-05-19 DOI: 10.1088/2050-6120/ac719a

Shuaici Cheng, Ran Sun, Zhuo-Hui Wu, Huihui Mei, Haocheng Yang, Qingqing Kong, Kuoxi Xu

引用次数: 3