Methods and Applications in Fluorescence最新文献_第9页

Pulse-shaped broadband multiphoton excitation for single-molecule fluorescence detection in the far field. 用于远场单分子荧光检测的脉冲型宽带多光子激发。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-01-19 DOI: 10.1088/2050-6120/aca87f

David Nobis, Henry G Sansom, Steven W Magennis

{"title":"Pulse-shaped broadband multiphoton excitation for single-molecule fluorescence detection in the far field.","authors":"David Nobis, Henry G Sansom, Steven W Magennis","doi":"10.1088/2050-6120/aca87f","DOIUrl":"https://doi.org/10.1088/2050-6120/aca87f","url":null,"abstract":"Multiphoton excitation of fluorescence has many potential advantages over resonant (one-photon) excitation, but the method has not found widespread use for ultrasensitive applications. We recently described an approach to the multiphoton excitation of single molecules that uses a pulse shaper to compress and tailor pulses from an ultrafast broadband laser in order to optimise the brightness and signal-to-background ratio following non-linear excitation. Here we provide a detailed description of the setup and illustrate its use and potential by optimising two-photon fluorescence of a common fluorophore, rhodamine 110, at the single-molecule level. We also show that a DNA oligonucleotide labelled with a fluorescent nucleobase analogue, tC, can be detected using two-photon FCS, whereas one-photon excitation causes rapid photobleaching. The ability to improve the signal-to-background ratio and to reduce the incident power required to attain a given brightness can be applied to the multiphoton excitation of any fluorescent species, from small molecules with low multiphoton cross sections to the brightest nanoparticles.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9660290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Quantity with quality. 数量与质量并重。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2023-01-03 DOI: 10.1088/2050-6120/aca5f6

David J S Birch, Marcia Levitus, Yves Mély

引用次数: 0

MINFLUX imaging of a bacterial molecular machine at nanometer resolution. 细菌分子机器的纳米分辨率MINFLUX成像。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-12-13 DOI: 10.1088/2050-6120/aca880

Alexander Carsten, Maren Rudolph, Tobias Weihs, Roman Schmidt, Isabelle Jansen, Christian A Wurm, Andreas Diepold, Antonio Virgilio Failla, Manuel Wolters, Martin Aepfelbacher

{"title":"MINFLUX imaging of a bacterial molecular machine at nanometer resolution.","authors":"Alexander Carsten, Maren Rudolph, Tobias Weihs, Roman Schmidt, Isabelle Jansen, Christian A Wurm, Andreas Diepold, Antonio Virgilio Failla, Manuel Wolters, Martin Aepfelbacher","doi":"10.1088/2050-6120/aca880","DOIUrl":"https://doi.org/10.1088/2050-6120/aca880","url":null,"abstract":"The resolution achievable with the established super-resolution fluorescence nanoscopy methods, such as STORM or STED, is in general not sufficient to resolve protein complexes or even individual proteins. Recently, minimal photon flux (MINFLUX) nanoscopy has been introduced that combines the strengths of STED and STORM nanoscopy and can achieve a localization precision of less than 5 nm. We established a generally applicable workflow for MINFLUX imaging and applied it for the first time to a bacterial molecular machinein situ, i.e., the injectisome of the enteropathogenY. enterocolitica. We demonstrate with a pore protein of the injectisome that MINFLUX can achieve a resolution down to the single molecule levelin situ. By imaging a sorting platform protein using 3D-MINFLUX, insights into the precise localization and distribution of an injectisome component in a bacterial cell could be accomplished. MINFLUX nanoscopy has the potential to revolutionize super-resolution imaging of dynamic molecular processes in bacteria and eukaryotes.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":"11 1","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10406921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation. 角蛋白固有荧光作为一种无创监测其糖化的机制。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-12-01 DOI: 10.1088/2050-6120/aca507

Rhona Muir, Shareen Forbes, David J S Birch, Vladislav Vyshemirsky, Olaf J Rolinski

引用次数: 0

Preparation, AIE and ESIPT behaviour, controllable solid-state fluorescence and application of Co²⁺probe based onα-cyanostilbene. 基于α-氰二苯乙烯的Co2+探针的制备、AIE和ESIPT行为、可控固态荧光及应用。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-11-25 DOI: 10.1088/2050-6120/aca378

Meihui Chen, Wenheng Huang, Yangping Li, Yongchun Chen, Dan Ji, Mingguang Zhu

{"title":"Preparation, AIE and ESIPT behaviour, controllable solid-state fluorescence and application of Co2+probe based onα-cyanostilbene.","authors":"Meihui Chen, Wenheng Huang, Yangping Li, Yongchun Chen, Dan Ji, Mingguang Zhu","doi":"10.1088/2050-6120/aca378","DOIUrl":"https://doi.org/10.1088/2050-6120/aca378","url":null,"abstract":"A simpleα-cyanostilbene-functioned salicylaldehyde-based Schiff-base probe, which exhibited outstanding 'aggregation-induced emission and excited state intramolecular proton transfer (AIE + ESIPT)' emission in solution, aggregation and solid states, was synthesized in high yield of 87%. Its solid-states with different morphologies emitted different fluorescence after crystallization in EtOH/H2O (1/2, v/v) mixtures or pure EtOH solvent. Besides, it exhibited an obvious spectro-photometrical fluorescence quenching for highly selective sensing of Co2+in THF/water system (ƒw= 60%, pH = 7.4), accompanied by an intense green fluorescence turn-off behavior under UV365nmillumination. The binding stochiometry between the ligand and Co2+was found to be 2:1, and the detection limit (DL) was calculated to be 0.41 × 10-8M. In addition, it could be applied to detect Co2+in real water samples and on silica gel testing strip.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40477947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A novel conjugated polymer fluorescence probe for the detection of copper ions in aqueous media. 一种用于检测水介质中铜离子的新型共轭聚合物荧光探针。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-11-16 DOI: 10.1088/2050-6120/ac9fa6

Lindan Gong, Lixiao Zhang, Rui Shen, Lin Zhang, Hengyuan Wang, Lu-An Fan

引用次数: 1

Comparative study of upconversion and photoacoustic measurements of Er³⁺/Yb³⁺doped La₂O₃phosphor under 980 nm. 980 nm下Er3+/Yb3+掺杂la2o3荧光粉上转换和光声测量的比较研究。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-11-16 DOI: 10.1088/2050-6120/ac9fa7

Minarul I Sarkar, Neeraj Kumar Mishra, Kaushal Kumar

{"title":"Comparative study of upconversion and photoacoustic measurements of Er3+/Yb3+doped La2O3phosphor under 980 nm.","authors":"Minarul I Sarkar, Neeraj Kumar Mishra, Kaushal Kumar","doi":"10.1088/2050-6120/ac9fa7","DOIUrl":"https://doi.org/10.1088/2050-6120/ac9fa7","url":null,"abstract":"The Er3+/Yb3+doped La2O3phosphor samples were synthesized by the combustion method and then photoluminescence and photoacoustic spectroscopic studies were done. Prepared samples were annealed at 800 °C, 1000 °C and 1300 °C and all samples were found in pure hexagonal phase as confirmed by XRD analysis. From FE-SEM images it is found that particle size increases with increase in annealing temperature. The frequency upconversion emission spectra of samples were recorded by exciting the sample with 980 nm diode laser and maximum emission intensity is obtained for the sample annealed at 1000 °C for 2 h. A photoacoustic cell was designed and wavelength dependent photoacoustic spectra were measured. The effect of sample storage time on radiative and non-radiative emission properties of sample was checked by measuring upconversion emission and photoacoustic spectra, simultaneously. It is observed that the emission intensity and photoacoustic signal both decreases with time. The maximum photoacoustic signal is obtained around 974 nm wavelength and it indicates its potential for photo-thermal therapy using infrared excitation.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40686724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Phasor-based multi-harmonic unmixing forin-vivohyperspectral imaging. 基于相量的体内高光谱成像多谐解混。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-11-09 DOI: 10.1088/2050-6120/ac9ae9

Alexander Vallmitjana, Paola Lepanto, Florencia Irigoin, Leonel Malacrida

{"title":"Phasor-based multi-harmonic unmixing forin-vivohyperspectral imaging.","authors":"Alexander Vallmitjana, Paola Lepanto, Florencia Irigoin, Leonel Malacrida","doi":"10.1088/2050-6120/ac9ae9","DOIUrl":"https://doi.org/10.1088/2050-6120/ac9ae9","url":null,"abstract":"Hyperspectral imaging (HSI) is a paramount technique in biomedical science, however, unmixing and quantification of each spectral component is a challenging task. Traditional unmixing relies on algorithms that need spectroscopic parameters from the fluorescent species in the sample. The phasor-based multi-harmonic unmixing method requires only the empirical measurement of the pure species to compute the pixel-wise photon fraction of every spectral component. Using simulations, we demonstrate the feasibility of the approach for up to 5 components and explore the use of adding a 6th unknown component representing autofluorescence. The simulations show that the method can be successfully used in typical confocal imaging experiments (with pixel photon counts between 101and 103). As a proof of concept, we tested the method in living cells, using 5 common commercial dyes for organelle labeling and we easily and accurately separate them. Finally, we challenged the method by introducing a solvatochromic probe, 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine (LAURDAN), intended to measure membrane dynamics on specific subcellular membrane-bound organelles by taking advantage of the linear combination between the organelle probes and LAURDAN. We succeeded in monitoring the membrane order in the Golgi apparatus, Mitochondria, and plasma membrane in the samein-vivocell and quantitatively comparing them. The phasor-based multi-harmonic unmixing method can help expand the outreach of HSI and democratize its use by the community for it does not require specialized knowledge.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33546813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photophysical, optical and lasing analysis of fluorinatedβ-keto carboxylate europium(III) complexes. 氟化β-酮羧酸铕(III)配合物的光物理、光学和激光分析。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-11-03 DOI: 10.1088/2050-6120/ac98f5

Savita Khatri, Pratibha Ahlawat, S P Khatkar, V B Taxak, Rajesh Kumar

{"title":"Photophysical, optical and lasing analysis of fluorinatedβ-keto carboxylate europium(III) complexes.","authors":"Savita Khatri, Pratibha Ahlawat, S P Khatkar, V B Taxak, Rajesh Kumar","doi":"10.1088/2050-6120/ac98f5","DOIUrl":"https://doi.org/10.1088/2050-6120/ac98f5","url":null,"abstract":"Six luminescent, bright red Eu(III) complexes with aβ-keto-carboxylic acid as prime ligand and N-donor aromatic systems as auxillary ligand were synthesised via ecologically efficient grinding method. The distinctive red peak (5D0 → 7F2) of Eu(III) ion is exhibited in emission spectra of all complexes. The luminescent properties of complexes were analysed through decay time, color coordinates, luminescence efficiency and Judd Ofelt parameters. The value of Ω2is found to be higher than Ω4which indicated hypersensitive nature of5D0 → 7F2transition. The results established the complexes as a strong contender for red light emitting display devices. The fluorescence branching ratios, stimulated emission cross section, gain band width and optical gain showed the good lasing strength of5D0 → 7F2transition of complexes. The complexes exhibited decent thermal stability and have optical energy band gap value in semiconductor range, thus can have relevance in optoelectronic devices. Energy transfer mechanism was investigated for complexes which affirmed the efficacious transfer of energy from ligands to Eu(III) ion. The synthesised complexes were also assayed for antimicrobial and antioxidant properties. All complexes are reported to show better antioxidant behaviour than the prime ligand and also exhibited upstanding antibacterial activities.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33519561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

One-pot ultrasonicated synthesized lead-less hybrid perovskite nanocrystals: structural, morphological and optical analysis. 一锅超声合成无铅杂化钙钛矿纳米晶体:结构、形态和光学分析。

IF 3.2 3区化学

Methods and Applications in Fluorescence Pub Date : 2022-10-18 DOI: 10.1088/2050-6120/ac9775

Rajan Kumar Singh, Sourabh Gouraha, Anupriya Singh, Neha Jain, Jai Singh

{"title":"One-pot ultrasonicated synthesized lead-less hybrid perovskite nanocrystals: structural, morphological and optical analysis.","authors":"Rajan Kumar Singh, Sourabh Gouraha, Anupriya Singh, Neha Jain, Jai Singh","doi":"10.1088/2050-6120/ac9775","DOIUrl":"https://doi.org/10.1088/2050-6120/ac9775","url":null,"abstract":"In contemporary years, hybrid lead halide perovskites nanocrystals (HPNCs) have emerged as core materials for low-cost solution-processable photovoltaic, light-emitting devices as well as in other optoelectronic fields, such as high-efficiency perovskite fluorescent quantum dots (quantum dot, QD). Although the high efficiency makes them an attractive active material, reducing the Pb-toxicity and enhancing the stability while sustaining the efficiency of the HPNCs devices is important for their successful commercialization in future. Here, we report for the first time the fabrication of excellent quality Pb-less, MAPb1-xSnxBr3(x = 0 to 0.50) perovskite NCs by one-pot ultrasonication method. Interestingly, an outstanding photoluminescence quantum yield (PLQY) of 94% and better lifetime performance than 100% Pb-based HPNCs is obtained for Pb-less HPNCs. The successful incorporation of Sn MAPb1-xSnxBr3HPNCs is confirmed by energy-dispersive x-ray (EDX) and x-ray photoelectron spectroscopy (XPS) analysis. Although the particle size for Pb-less HPNCs was different, the change in morphology and structure was minimal as confirmed by transmission electron microscopy (TEM) analysis. The optical analysis indicated bandgap tuning, which is evident by the blue shift of the band edge in absorbance spectra and photoluminescence peak after incorporating Sn2+. To the best of our knowledge, this is the highest achieved PLQY for Sn-substituted hybrid Pb-based HPNCs. The synthesis by using one pot ultrasonication method might be helpful for large-scale HPNCs production and can pave the way for future research on less-toxic and stable alternatives to Pb-based HPNCs.","PeriodicalId":18596,"journal":{"name":"Methods and Applications in Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33486930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0