教程:多光子显微镜推进神经科学研究。

IF 2.4 3区化学 Q3 CHEMISTRY, ANALYTICAL

Methods and Applications in Fluorescence Pub Date : 2023-02-22 DOI:10.1088/2050-6120/acba66

Iván Coto Hernández, Jenny Yau, Lars Rishøj, Nanke Cui, Steven Minderler, Nate Jowett

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引用次数: 0

摘要

多光子显微镜(MPM)采用超快红外激光对活体生物样品进行高分辨率深三维成像。本教程的目标是为新手显微镜开发人员和生命科学用户提供MPM成像的实用指南。讨论了MPM的原理、显微镜设置和标记策略。利用MPM实现前所未有的全植体成像深度和通过哺乳动物神经系统植入式玻璃窗的活体成像。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Tutorial: multiphoton microscopy to advance neuroscience research.

Multiphoton microscopy (MPM) employs ultrafast infrared lasers for high-resolution deep three-dimensional imaging of live biological samples. The goal of this tutorial is to provide a practical guide to MPM imaging for novice microscopy developers and life-science users. Principles of MPM, microscope setup, and labeling strategies are discussed. Use of MPM to achieve unprecedented imaging depth of whole mounted explants and intravital imaging via implantable glass windows of the mammalian nervous system is demonstrated.

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来源期刊

Methods and Applications in Fluorescence CHEMISTRY, ANALYTICALCHEMISTRY, PHYSICAL&n-CHEMISTRY, PHYSICAL

CiteScore

6.20

自引率

3.10%

发文量

期刊介绍： Methods and Applications in Fluorescence focuses on new developments in fluorescence spectroscopy, imaging, microscopy, fluorescent probes, labels and (nano)materials. It will feature both methods and advanced (bio)applications and accepts original research articles, reviews and technical notes.