A new twist on PIFE: photoisomerisation-related fluorescence enhancement.

IF 2.4 3区化学 Q3 CHEMISTRY, ANALYTICAL

Methods and Applications in Fluorescence Pub Date : 2023-10-12 DOI:10.1088/2050-6120/acfb58

Evelyn Ploetz, Benjamin Ambrose, Anders Barth, Richard Börner, Felix Erichson, Achillefs N Kapanidis, Harold D Kim, Marcia Levitus, Timothy M Lohman, Abhishek Mazumder, David S Rueda, Fabio D Steffen, Thorben Cordes, Steven W Magennis, Eitan Lerner

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Abstract

PIFE was first used as an acronym for protein-induced fluorescence enhancement, which refers to the increase in fluorescence observed upon the interaction of a fluorophore, such as a cyanine, with a protein. This fluorescence enhancement is due to changes in the rate ofcis/transphotoisomerisation. It is clear now that this mechanism is generally applicable to interactions with any biomolecule. In this review, we propose that PIFE is thereby renamed according to its fundamental working principle as photoisomerisation-related fluorescence enhancement, keeping the PIFE acronym intact. We discuss the photochemistry of cyanine fluorophores, the mechanism of PIFE, its advantages and limitations, and recent approaches to turning PIFE into a quantitative assay. We provide an overview of its current applications to different biomolecules and discuss potential future uses, including the study of protein-protein interactions, protein-ligand interactions and conformational changes in biomolecules.

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PIFE的一个新转折：与光异构化相关的荧光增强。

PIFE最初被用作蛋白质诱导荧光增强的缩写，指的是在荧光团（如花青）与蛋白质相互作用时观察到的荧光增加。这种荧光增强是由于顺式/反式光异构化速率的变化。现在很清楚，这种机制通常适用于与任何生物分子的相互作用，在这篇综述中，我们建议根据其基本工作原理将PIFE重命名为与光异构化相关的荧光增强，保持PIFE首字母缩写的完整性。我们讨论了菁荧光团的光化学、PIFE的机制、其优点和局限性，以及将PIFE转化为定量分析的最新方法。我们概述了它目前在不同生物分子中的应用，并讨论了未来的潜在用途，包括蛋白质-蛋白质相互作用、蛋白质-配体相互作用和生物分子构象变化的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods and Applications in Fluorescence CHEMISTRY, ANALYTICALCHEMISTRY, PHYSICAL&n-CHEMISTRY, PHYSICAL

CiteScore

6.20

自引率

3.10%

发文量

期刊介绍： Methods and Applications in Fluorescence focuses on new developments in fluorescence spectroscopy, imaging, microscopy, fluorescent probes, labels and (nano)materials. It will feature both methods and advanced (bio)applications and accepts original research articles, reviews and technical notes.