Microbial Cell Factories最新文献

{"title":"Improving the bacillomycin L production in Bacillus amyloliquefaciens by atmospheric and room-temperature plasma combined with Box-Behnken design.","authors":"Andong Zhang, Bin Yang, Yudong Ma, Ruolin Li, Zhiwei Zhou, Chen Luo, Yiyang Zhang, Yuhan Zhang, Ya Dan, Qun Sun","doi":"10.1186/s12934-025-02774-1","DOIUrl":"10.1186/s12934-025-02774-1","url":null,"abstract":"<p><p>Bacillomycin L, a cyclic lipopeptide derived from Bacillus amyloliquefaciens, has great potential for developing biochemical phytofungicides. In this study, a mutant M86 with high anti-Botrytis cinerea activity was obtained by atmospheric and room-temperature plasma (ARTP) mutagenesis from the original strain 1841, with bacillomycin L yield increasing from 244.22 to 415.89 mg/L. Mass spectrometry analysis identified that the main active compounds were C14-, C15-, and C16-bacillomycin L. Re-sequencing M86 showed that ARTP mutagenesis resulted in the effective mutations in sigma factors and ABC transporter proteins. Transcriptome sequencing further revealed the significant up-regulation of the bmyDABC and ytrBCC2DEF gene cluster involved in bacillomycin L synthesis and transporter, respectively, in M86. Bacillomycin L yield was further boosted to 676.47 mg/L after optimizing the fermentation medium by adjusting glycine, serine, and K₂HPO₄ concentrations. Bacillomycin L exhibited broad inhibitory activity against 17 fungi and nine bacterial species. This investigation provides a foundation for bacillomycin L production and application.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"144"},"PeriodicalIF":4.3,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Building a cell-factory in Crithidia fasciculata: a bio-sustainable system to produce high-value polyunsaturated fatty acids.","authors":"Michela Cerone, Louise L Major, Terry K Smith","doi":"10.1186/s12934-025-02760-7","DOIUrl":"10.1186/s12934-025-02760-7","url":null,"abstract":"<p><p>Fatty acids (FAs) are a class of diverse biomolecules that are essential for a wide variety of biological processes. Recently, ω-3 and ω-6 polyunsaturated FAs (PUFAs) have received a lot of attention for their benefit and essentiality to human health. Their demand is constantly increasing alongside the ever-decreasing availability of sources of natural PUFAs, such as some plants and marine ecosystems, which are now endangered by agribusiness and climate change. Most of the novel microbial synthetic platforms to produce ω-3 and ω-6 PUFAs (e.g. yeast, microalgae, oleaginous microorganisms, bacteria etc.) are costly. On the other hand, industrial processes based on chemical total synthesis of PUFAs is often challenging. Here, we propose an innovative and bio-sustainable method to make ω-3 and ω-6 PUFAs by building a cell-factory in Crithidia fasciculata, using genetic manipulation of endogenous FA desaturases and elongases, supplementation of the culture media with cheap sources of fats (such as used cooking oils), and culturing temperature reduction. In these unusual environments, C. fasciculata show high adaptability: the cells grow rapidly producing large biomass from small volumes of culture, scavenge and remodel unusual fat sources, as well as biosynthesising high value FAs and PUFAs de novo. By genetic manipulation, allowing overexpression of either the endogenous Δ6-desaturases or Δ4-desaturases, in conjunction with the endogenous elongase Elo4, high value ω-6 and ω-3 PUFAs were yielded to a maximum of 50% of the total FA content of the cells. These bioengineered C. fasciculata provide a low-cost and bio-sustainable platform to increase the production of essential PUFAs which can be introduced into the food chain at lower costs.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"142"},"PeriodicalIF":4.3,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183914/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing an anti-CRISPR protein for powering CRISPR/Cas9-mediated genome editing in undomesticated Bacillus strains.","authors":"Man Su Kim, Da-Eun Jeong, Soo-Keun Choi","doi":"10.1186/s12934-025-02776-z","DOIUrl":"10.1186/s12934-025-02776-z","url":null,"abstract":"<p><strong>Background: </strong>Wild-type Bacillus strains have significant industrial and medical value, but their effective utilization often requires strain improvement. The CRISPR/Cas9 system has become the primary tool for genome editing, allowing precise introduction of desired mutations at specific chromosomal locations. However, the practical application of CRISPR/Cas9 in most wild-type Bacillus strains remains challenging due to cellular toxicity resulting from Cas9/sgRNA activity. Therefore, controlling Cas9 toxicity is essential for the widespread application of the CRISPR/Cas9 system in wild-type Bacillus strains.</p><p><strong>Results: </strong>We employed AcrIIA4, an anti-CRISPR protein that inhibits the Cas9/sgRNA ribonucleoprotein complex from interacting with DNA, to mitigate Cas9/sgRNA-mediated toxicity, thereby enabling CRISPR/Cas9-based genome editing in wild-type strains. The newly constructed CRISPR/anti-CRISPR (CAC) plasmids harbor both cas9 and acrIIA4 genes controlled by the P_spac and P_xyl promoters, respectively, along with the repressor genes lacI and xylR. This design allows precise control of Cas9 activity through inducers. Xylose, which induces AcrIIA4 expression, effectively alleviated Cas9/sgRNA-mediated toxicity during transformation. Under xylose induction, the CAC plasmid led to a remarkable 139-fold increase in the transformation efficiency of wild-type Bacillus subtilis compared to a plasmid lacking anti-CRISPR. Meanwhile, IPTG induction promoted Cas9 expression, facilitating efficient genome editing. Upon IPTG induction, the genome editing efficiency in wild-type B. subtilis increased from 0 to 95.8% in transformants carrying the CAC plasmid. Importantly, our findings extend beyond B. subtilis, revealing that the anti-CRISPR protein dramatically enhanced transformation and genome editing efficiencies in Bacillus pumilus. Moreover, we demonstrated that the CAC system successfully enabled the generation of spo0A mutants in Bacillus mojavensis, Bacillus tequilensis, and Paenibacillus polymyxa.</p><p><strong>Conclusions: </strong>In this study, we developed a CAC system that utilizes the anti-CRISPR protein AcrIIA4 to reduce Cas9/sgRNA-mediated toxicity in Bacillus strains. This system enables precise control of AcrIIA4 and Cas9 expression through inducers, significantly enhancing the efficiency of transformation and genome editing in wild-type Bacillus strains. Therefore, the CAC system stands as a powerful tool to facilitate genome editing in diverse wild-type Bacillus species.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"143"},"PeriodicalIF":4.3,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a lysine biosensor for the dynamic regulation of cadaverine biosynthesis in E. coli.","authors":"Mingjie Li, Haorong Chen, Yukun Chen, Pengyu Chen, Shuchun Liu, Shuwen Liu, Tingyi Wen","doi":"10.1186/s12934-025-02772-3","DOIUrl":"10.1186/s12934-025-02772-3","url":null,"abstract":"<p><strong>Background: </strong>As one of the most promising monomers of biobased polyamides, cadaverine has wide industrial application prospects. However, in microbial cadaverine fermentation with glucose as the sole carbon source, the impaired coordination between precursor (lysine) utilization and cytotoxic cadaverine accumulation has been identified as the primary bottleneck limiting high-yield biosynthesis. Here, we developed a lysine biosensor in Escherichia coli to dynamically regulate cadaverine biosynthesis.</p><p><strong>Results: </strong>In this study, we developed a lysine biosensor based on the lysine transporter protein LysP, the transcription activator CadC, and the GFPuv gene under the control of the P_cad promoter. However, the engineered lysine biosensor system had a low dynamic range and a narrow pH operating range. Therefore, a multilevel optimization strategy, which included the introduction of key point mutations and engineered promoter modifications, were introduced to improve the performance of the biosensor, resulting in significant improvements in the dynamic range and lysine response. Moreover, we engineered a cadaverine-producing E. coli strain by increasing the supply of the lysine precursor, overexpressing key cadaverine synthesis genes, and knocking out genes related to metabolic bypass. The lysine biosensor was subsequently implemented to dynamically regulate cadaverine biosynthesis, resulting in a 48.10% increase in the production titre (33.19 g/L) and a 21.2% increase in cell growth compared with those resulting from the strain with constitutive expression.</p><p><strong>Conclusion: </strong>This is the first report in which a lysine biosensor constructed in E. coli could dynamically regulate cadaverine synthesis to improve its yield and biomass. This strategy provides new insights into the metabolic engineering of lysine and its derivatives in E. coli.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"141"},"PeriodicalIF":4.3,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth-independent CLA production by Bifidobacterium breve JKL2022 and the potential transcriptional regulatory role of TetR in linoleic acid isomerase expression.","authors":"Arxel G Elnar, Geun-Bae Kim","doi":"10.1186/s12934-025-02766-1","DOIUrl":"10.1186/s12934-025-02766-1","url":null,"abstract":"<p><strong>Background: </strong>Microbial production of conjugated linoleic acid (CLA) has garnered wide attention for the possibility to increase the CLA content in food products, therefore achieving higher concentrations of beneficial compounds for consumers. However, this approach has only been done using metabolically active cells, particularly in Bifidobacterium spp., thus being a major limitation given the anaerobic and fastidious nature of bifidobacteria. In this study, we aimed to investigate the capacity of Bifidobacterium breve JKL2022 (KACC81214BP) to convert free linoleic acid (LA) into CLA using growing cells and postbiotic preparations (washed cells and crude protein extracts) as catalysts.</p><p><strong>Results: </strong>Bifidobacterium breve JKL2022 demonstrated high CLA production as early as 6 h and continued to increase until 12-15 h of incubation. Moreover, CLA production was observed in JKL2022 washed cells (97.42 ± 3.64%) and crude protein fractions (33.87 ± 4.05%- 103.65 ± 2.70%) obtained after cell lysis, highlighting its superior CLA-converting activity compared to the B. breve JCM strains. In vitro CLA reaction conditions were optimal at pH 7.0, following the first-order kinetics within the first 5 min of reaction, and the extraction efficiency of the isopropanol-hexane protocol increased after adjusting the pH to 5.0-5.5. Finally, RT-qPCR and in silico analysis revealed a strong correlation between the expression levels of lai (JKL2022_00014) and tetR (JKL2022_00217) genes, suggesting the potential role of TetR in upregulating the lai gene expression in JKL2022 that could explain the LA conversion in washed JKL2022 cells.</p><p><strong>Conclusions: </strong>The ability of B. breve JKL2022 strain to convert free LA to CLA during growth, as well as using washed cells and crude protein extracts, suggests strain specificity and superior enzymatic activity. In addition to its potential application as a probiotic strain with CLA-enhancing properties, washed JKL2022 cells or crude protein extracts can be developed as postbiotic preparations for the same purpose.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"140"},"PeriodicalIF":4.3,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing the power of Bacillus subtilis MR42: direct production of defined xylooligosaccharides (XOS) from corn stalks with enhanced prebiotic properties.","authors":"Lusha Wei, Shuo Sun, Yuan Chen, Xin An, Hanning Wu, Yifei Wu","doi":"10.1186/s12934-025-02756-3","DOIUrl":"10.1186/s12934-025-02756-3","url":null,"abstract":"<p><p>The production of value-added bioproducts from renewable carbon sources has gained increasing attention in recent years. The aim of this study was to develop economical approaches for the production of prebiotic XOS with defined degree of polymerization (DP) by Bacillus subtilis MR42 from corn stalks. The MR42 strain has been genetically modified to delete the xynC gene, which enables it to convert xylan into specific XOS without generating xylose as a byproduct. Under optimal conditions, a high yield of XOS (703.4 mg/g xylan) was obtained. The purified XOS were further characterized using various techniques including TLC, HPLC, ESI-MS and ¹HNMR spectroscopy. The analysis revealed that the XOS primarily consisted of xylotriose (257 ± 0.14 mg/g XOS), xylotetraose (267 ± 0.24 mg/g XOS) and xylopentaose (289 ± 0.29 mg/g XOS), accounting for 81.3% of the total XOS. The prebiotic effect of XOS was evaluated by examining its impact on the growth of Lactobacillus, the production of short-chain fatty acids, and its antioxidant activity. B. subtilis MR42 showed a promising ability to produce XOS of DP 3-5, without the generation of xylose.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"139"},"PeriodicalIF":4.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: A highly efficient heterologous expression platform to facilitate the production of microbial natural products in Streptomyces.","authors":"Xiuling Wang, Ping Lin, Qiyao Shen, Xueyan Feng, Shouying Xu, Qijun Zhang, Yang Liu, Cailing Ren, Daojing Yong, Qiong Duan, Liujie Huo, Youming Zhang, Gang Li, Jun Fu, Ruijuan Li","doi":"10.1186/s12934-025-02761-6","DOIUrl":"10.1186/s12934-025-02761-6","url":null,"abstract":"","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"138"},"PeriodicalIF":4.3,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12178030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Red Sea- and Mediterranean Sea-derived Actinomycetes for Antimicrobial and Antitumor activities: LC-ESI-HRMS-based Metabolomics Study.","authors":"Mahmoud A Abdel-Razik, Ahmed F Azmy, Tarek Dishisha, Ahmed O El-Gendy, Adlin Afzan, Nurkhalida Kamal, Ahmed Tawfike, Mohamed Sebak","doi":"10.1186/s12934-025-02759-0","DOIUrl":"10.1186/s12934-025-02759-0","url":null,"abstract":"","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"136"},"PeriodicalIF":4.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity reduction in continuous, high productivity ethanol fermentation by Parageobacillus thermoglucosidasius using in situ microbubble gas stripping.","authors":"Christopher Ibenegbu, William B Zimmerman, Michael Hines, Pratik D Desai, H C Hemaka Bandulasena, David J Leak","doi":"10.1186/s12934-025-02754-5","DOIUrl":"10.1186/s12934-025-02754-5","url":null,"abstract":"<p><p>Ethanol concentrations above 4% (v/v) are required for economic bioethanol production due to the cost of recovery from dilute solutions. Although thermophilic bacteria have many potential advantages over Saccharomyces cerevisiae as process organisms for second generation bioethanol production, they are known to be less tolerant to ethanol, typically to concentrations less than 4% (v/v). To address this issue we have investigated the application of in situ gas-stripping of ethanol using microbubbles to increase the surface area per unit volume of gas, using fed-batch and continuous cultures of the engineered ethanologenic thermophile Parageobacillus thermoglucosidasius TM242. By using microbubbles generated at room temperature using a Desai-Zimmerman Fluid Oscillator, we initially operated a mixed batch and fed-batch fermentation, followed by a continuous fermentation and finally a chemostat fermentation, under conditions which would have generated in excess of 4% (v/v) ethanol. In all cases, gas stripping maintained the actual dissolved ethanol concentration below, or close to toxic levels. As the focus of this study was on demonstrating the efficiency of in situ microbubble gas stripping, to simplify the operation the latter two processes involved a combination of produced and supplemented ethanol, with the chemostat culture producing a nominal maximum 7.1% v/v based on glucose used (5.1-5.3% (v/v) based on ethanol recovered). This offers a practical way to produce second generation bio-ethanol from thermophiles.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"137"},"PeriodicalIF":4.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12177972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liposome-encapsulated antibiotics and biosurfactants: an effective strategy to boost biofilm eradication in cooling towers.","authors":"Marcus Vinícius Dias-Souza, Ihtisham Ul Haq, Sérgio Pagnin, Andrea Azevedo Veiga, Vera Lúcia Dos Santos","doi":"10.1186/s12934-025-02746-5","DOIUrl":"10.1186/s12934-025-02746-5","url":null,"abstract":"<p><p>An excessive amount of water is needed for cooling towers in oil refineries to cool the machinery. However, water has been observed to favor microbial growth and biofilms significantly. The microbial biofilms are usually treated with synthetic biocides, which are ineffective and generate toxic by-products harmful to the environment. This study explores using rhamnolipid and free or encapsulated antimicrobials in liposomes to control several bacterial species exhibiting low antimicrobial susceptibility in planktonic and biofilm forms. The antimicrobial efficacy of rhamnolipid was evaluated through minimum inhibitory concentration (MIC) tests, showing values between 0.244 and 31.25 µg/mL. Biofilm inhibition assays revealed that rhamnolipid significantly reduced biofilm viability, performing comparably to meropenem and more effectively than chloramphenicol. Liposomes were produced with initial diameters of 100 and 200 nm, and encapsulation efficiencies were 56.7% for rhamnolipid, 47.3% for meropenem, and 31.25% for chloramphenicol. Among the formulations, 100 nm rhamnolipid-loaded liposomes exhibited the highest antibiofilm efficacy, achieving up to 92% biofilm reduction in Stenotrophomonas maltophilia 94 (p < 0.01). Meropenem liposomes of 100 nm also performed better than their 200 nm counterparts, with up to 85% reduction in Pseudomonas aeruginosa biofilms (p < 0.05). No significant size-dependent differences were observed for chloramphenicol liposomes, with maximum inhibition around 60% at both sizes. Long-term stability and antibiofilm activity were evaluated exclusively for S. maltophilia 94 over 90 days of refrigerated storage (4 °C). Dynamic light scattering revealed significant vesicle size increases over time for both formulations (p < 0.05), yet their antibiofilm activity remained stable. Rhamnolipid liposomes (100 nm) maintained significantly higher efficacy than 200 nm vesicles throughout the period (p < 0.01). Meropenem liposomes retained considerable activity, though a moderate decrease was noted after 60 days. Scanning electron microscopy (SEM) at days 0 and 90 confirmed the antimicrobial impact of liposomal treatments: biofilms showed disrupted architecture, reduced extracellular matrix, and evident morphological damage to bacterial cells, supporting quantitative results. These findings demonstrate that liposome-encapsulated rhamnolipids and antibiotics are effective against resilient biofilms. The successful formulation and long-term stability of rhamnolipid liposomes highlight their potential as a sustainable and eco-friendly alternative for industrial biofilm control, reducing reliance on conventional biocides and minimizing environmental impact.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"135"},"PeriodicalIF":4.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}