Microbial Cell Factories最新文献

{"title":"Lecanicillium aphanocladii-derived copper oxide nanoparticles as a green nano-fungicide enhancing molecular and antioxidant defense against fusarium wilt in potato.","authors":"Noha A El-Sebaii, Fatmah A Safhi, Mohamed Halawa, Mai A El-Esawy, Doaa E Elsherif","doi":"10.1186/s12934-026-02970-7","DOIUrl":"10.1186/s12934-026-02970-7","url":null,"abstract":"","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147645736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterologous expression of 1,4-benzoquinone reductases from white-rot fungi in Saccharomyces cerevisiae confers protection against p-benzoquinone toxicity.","authors":"Mateo Bello-Villarino, Leif J Jönsson","doi":"10.1186/s12934-026-03001-1","DOIUrl":"10.1186/s12934-026-03001-1","url":null,"abstract":"<p><strong>Background: </strong>Ethanolic fermentation by Saccharomyces cerevisiae is a key part of biochemical conversion of lignocellulosic feedstocks. Although abundant, lignocellulose typically requires pretreatment for efficient bioconversion. During pretreatment, inhibitory compounds are formed. p-Benzoquinone (p-BQ) is a lignin-derived inhibitor that is highly toxic to S. cerevisiae. White-rot fungi, efficient degraders of lignin, produce 1,4-benzoquinone reductases (QRDs), which have been suggested to play a role in defense against oxidative stress caused by quinones formed during lignin biodegradation. The hypothesis that QRDs from white-rot fungi can be used to engineer S. cerevisiae with improved resistance against quinone toxicity was explored in experiments with QRD-encoding genes, with and without an N-terminal putative leader sequence, the function of which has not yet been deciphered.</p><p><strong>Results: </strong>Constitutive recombinant expression of QRDs from the white-rot fungi Trametes versicolor and Phanerochaete chrysosporium in S. cerevisiae resulted in increased tolerance against p-BQ toxicity compared to an empty vector control containing insert-less plasmid, with QRD-expressing constructs requiring 34 to 42% less time to reach half-time of maximum growth in p-BQ conditions adverse for the growth of the empty vector control (10 mg L^- 1). All QRD constructs were able to overcome p-BQ toxicity in concentrations that would otherwise be strongly inhibitory (20 mg L^- 1). Full-length constructs of both T. versicolor and P. chrysosporium QRD typically exhibited 1.5 times higher specific activity than leaderless counterparts and were better detoxifiers. An inverse correlation between QRD enzymatic activity and the time necessary to reach half-time of maximum growth (R² = 0.954) was observed during strongly inhibitory conditions. Transformants expressing QRD exhibited maintained glycerol and ethanol yields with increasing p-BQ concentrations, up to 0.44 g g^- 1 ethanol at 10 mg L^- 1 p-BQ, whereas the empty vector control exhibited increased glycerol yield and decreased ethanol yield, amounting to 0.15 g g^- 1 ethanol at 10 mg L^- 1 p-BQ.</p><p><strong>Conclusions: </strong>Heterologous expression of QRDs from white-rot fungi in S. cerevisiae confers protection against p-BQ toxicity. This investigation supports a role of QRD in the protection against toxic quinones and proposes a biodetoxification strategy against lignin-derived inhibitory p-BQ which can occur simultaneously with fermentation of sugars into ethanol in S. cerevisiae.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13088443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo biosynthesis of alpinetin enhanced by directed evolution of 5-O-methyltransferase.","authors":"Bo Peng, Ziwei Wang, Lili Zhang, Matthew R Groves, Kristina Haslinger","doi":"10.1186/s12934-026-02996-x","DOIUrl":"https://doi.org/10.1186/s12934-026-02996-x","url":null,"abstract":"","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147628020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inactivation of the sco2730/2731 copper chaperone-transporter system in Streptomyces coelicolor and its orthologs in Streptomyces venezuelae, together with chromosomal end deletion, greatly enhances secondary metabolism.","authors":"Gemma Fernández-García, Paula Valdés-Chiara, Paula García-Cancela, Nathaly González-Quiñónez, Juan Serna-Diestro, Felipe Lombó, María Montes-Bayón, Ángel Manteca","doi":"10.1186/s12934-026-03000-2","DOIUrl":"https://doi.org/10.1186/s12934-026-03000-2","url":null,"abstract":"","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147628043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal-fungal interaction between Sanghuangporus vaninii and its endophytic Fusarium solani rewires host secondary metabolism to boost bioactive metabolite production.","authors":"Yanjun Ma, Lanlan Yu, Pengcheng Di, Xuetai Zhu, Xiaoyan Ma, Weibao Kong","doi":"10.1186/s12934-026-02994-z","DOIUrl":"https://doi.org/10.1186/s12934-026-02994-z","url":null,"abstract":"","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147618679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomics analysis identifies folding and secretion related genes for improving monoclonal antibody production in Thermothelomyces heterothallica C1.","authors":"Mari Mäkinen, Antti Aalto, Tiina Pakula, Marilyn G Wiebe, Anne Huuskonen, Marika Vitikainen, Mari Valkonen, Sami Havukainen, Ellinor Englund, Veera Korja, Mark Emalfarb, Noelia Valbuena Crespo, Ronen Tchelet, Markku Saloheimo","doi":"10.1186/s12934-026-02989-w","DOIUrl":"https://doi.org/10.1186/s12934-026-02989-w","url":null,"abstract":"","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147608964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Streptomyces shaoguanensis sp. nov.: elucidating the mechanisms of efficient chicken feather degradation and its potential for biofertilizer development.","authors":"Di Zhou, Weibin Zheng, Yijie Li, Ziqi Zhang, Xia Ding, Ye Ke","doi":"10.1186/s12934-026-02990-3","DOIUrl":"10.1186/s12934-026-02990-3","url":null,"abstract":"","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"25 1","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13023109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147521355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming prokaryotic toxicity: a SUMO-fused secretory platform in Komagataella phaffii for high-yield production of phage holin Hol41.","authors":"Zhichuang Huo, Fangfang Wang, Yanze He, Zhihui Chang, Shasha Feng, Jinzhe Kang, Cuihong Zhong, Yongying Zhang, Yuxiang Shi","doi":"10.1186/s12934-026-02983-2","DOIUrl":"10.1186/s12934-026-02983-2","url":null,"abstract":"","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13147679/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147530811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the genetic toolkit: adenine and cytosine base editors for gene disruption in Aspergillus niger.","authors":"Guoliang Yuan, Shuang Deng, Ziyu Dai, Beth A Hofstad, Kyle R Pomraning","doi":"10.1186/s12934-026-02979-y","DOIUrl":"10.1186/s12934-026-02979-y","url":null,"abstract":"","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13141642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147521374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual beneficial activities of Paenibacillus ottowii NPDY10: glyphosate degradation, modulation of soil microbial communities, and antagonism against phytopathogenic fungi.","authors":"Yun Deng, Yuqing Niu, Qing Xu, DongRong Xie, Yan Su, DuanHua Liu, Yong-He Chen, YongHe Han, Dagang Tian","doi":"10.1186/s12934-026-02991-2","DOIUrl":"10.1186/s12934-026-02991-2","url":null,"abstract":"<p><strong>Background: </strong>The widespread use and environmental persistence of the herbicide glyphosate raise significant concerns for human and ecological health. Developing efficient and sustainable remediation strategies is therefore crucial. Microbial degradation and biocontrol are two promising approaches. This study aimed to isolate and characterize a bacterial strain capable of both degrading glyphosate and antagonizing soil-borne fungal pathogens, and to evaluate its impact on soil microbial communities.</p><p><strong>Results: </strong>This study isolated and characterized a novel bacterial strain, NPDY10, identified as Paenibacillus ottowii, which exhibits dual beneficial functions: efficient glyphosate degradation and broad-spectrum antagonism against soil-borne fungal pathogens. Under optimal conditions (pH 7-7.5, 30-35 °C), strain NPDY10 completely degraded 400 mg L⁻¹ of glyphosate within four days, utilizing the herbicide as a carbon source. Metabolic profiling via UPLC-MS/MS confirmed glyphosate degradation via the aminomethylphosphonic acid (AMPA) pathway In a rice paddy field trial, the application of NPDY10 reduced glyphosate levels in paddy water, and a visible foam layer formed, serving as a visual indicator of active metabolism. Furthermore, strain NPDY10 demonstrated broad-spectrum antifungal activity in vitro, inhibiting 17 plant-pathogenic fungi with inhibition rates ranging from 60.00% to 92.00%. Analysis of soil microbial communities in a subsequent maize cultivation season revealed that NPDY10 treatment increased bacterial diversity and suppressed the relative abundance of pathogenic fungal genera such as Fusarium and Gibberella.</p><p><strong>Conclusion: </strong>Paenibacillus ottowii NPDY10 represents a promising multi-functional agent for sustainable agriculture, potentially combining glyphosate bioremediation with direct and indirect biocontrol effects through modulation of soil microbial communities, thereby contributing to improved soil health.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":" ","pages":""},"PeriodicalIF":4.9,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13141297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147521407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}