Microbial Cell Factories最新文献

{"title":"Engineered yeast Yarrowia lipolytica as a chassis for biosynthesis of fatty acids from mannitol and macroalgal biomass extracts.","authors":"Mateusz Szczepańczyk, Dorota A Rzechonek, Adam Dobrowolski, Aleksandra M Mirończuk","doi":"10.1186/s12934-025-02699-9","DOIUrl":"10.1186/s12934-025-02699-9","url":null,"abstract":"<p><strong>Background: </strong>Yarrowia lipolytica possesses the capability to utilize many unconventional carbon sources, such as crude glycerol, alkanes and fatty acids. Despite producing polyols, such as erythritol, arabitol and mannitol, the re-utilization of mannitol is not as efficient as erythritol utilization. Genes involved in mannitol uptake and metabolism in Y. lipolytica remain undescribed. However, deletion of the EYD1 gene (YALI0F01650g), believed to encode erythritol dehydrogenase, has been found to result in a high rate of growth on media containing mannitol as the sole carbon source. Therefore this unique feature was used for further fermentation studies on media containing macroalgal mannitol extracts, obtained from the brown alga Fucus vesiculosus, to produce value-added products.</p><p><strong>Results: </strong>The obtained strain AJD Δeyd1Dga1 was able to uptake pure and algal mannitol efficiently and produce high amounts of lipids, thanks to overexpression of the DGA1 gene (YALI0E32769g), encoding diacylglycerol (DAG) acyltransferase. The lipid content reached almost 32% of the overall dry biomass as compared to the wild type strain, where this value was more than 4 times lower. Additionally, the biomass at the end of the experiment was the highest among all of the tested strains, reaching 12.67 g/L, more than 50% higher than the control strain.</p><p><strong>Conclusions: </strong>The results of this study shed new light on the potential for the yeast Y. lipolytica to utilize macroalgae biomass as a carbon source for production of value-added products, including biomass and lipids. Moreover, the increased mannitol utilization capabilities can provide new insight into mannitol metabolism, including its uptake, which is especially crucial, as the metabolic pathways for all polyols produced by this organism seem to be closely intertwined.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"72"},"PeriodicalIF":4.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing D-lactic acid production from methane through metabolic engineering of Methylomonas sp. DH-1.","authors":"Seungwoo Cha, Jae-Hwan Jo, Jong Kwan Lee, Wooyoung Park, Myounghoon Moon, Gwon Woo Park, Min-Sik Kim, Ji-Sook Hahn","doi":"10.1186/s12934-025-02695-z","DOIUrl":"10.1186/s12934-025-02695-z","url":null,"abstract":"<p><strong>Background: </strong>Methane is an abundant and low-cost carbon source with great potential for conversion into value-added chemicals. Methanotrophs, microorganisms that utilize methane as their sole carbon and energy source, present a promising platform for biotechnological applications. This study aimed to engineer Methylomonas sp. DH-1 to enhance D-LA production through metabolic pathway optimization during large-scale cultivation.</p><p><strong>Results: </strong>In this study, we regulated the expression of D-lactate dehydrogenase (D-LDH) using a Ptac promoter with IPTG induction to mitigate the toxic effects of lactate accumulation. To further optimize carbon flow away from glycogen, the glgA gene was deleted. However, this modification led to growth inhibition, especially during scale-up, likely due to the accumulation of ADP-glucose caused by the rewired carbon flux under carbon-excess conditions. Deleting the glgC gene, which encodes glucose 1-phosphate adenylyltransferase, alleviated this issue. The final optimized strain, JHM805, achieved a D-LA production of 6.17 g/L in a 5-L bioreactor, with a productivity of 0.057 g/L/h, marking a significant improvement in D-LA production from methane.</p><p><strong>Conclusions: </strong>The metabolic engineering strategies employed in this study, including the use of an inducible promoter and alleviation of ADP-glucose accumulation toxicity, successfully enhanced the ability of the strain to produce D-LA from methane. Furthermore, optimizing the bioreactor fermentation process through methane and nitrate supplementation resulted in a significant increase in both the titer and productivity, exceeding previously reported values.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"70"},"PeriodicalIF":4.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The catabolic nature of fermentative substrates influences proteomic rewiring in Escherichia coli under anoxic growth.","authors":"Huda Momin, Deepti Appukuttan, K V Venkatesh","doi":"10.1186/s12934-025-02658-4","DOIUrl":"10.1186/s12934-025-02658-4","url":null,"abstract":"<p><strong>Background: </strong>During anaerobic batch fermentation of substrates by Escherichia coli, there is a decline in cell proliferation rates and a huge demand is placed on cellular proteome to cater to its catabolic and anabolic needs under anoxic growth. Considering cell growth rates as a physiological parameter, previous studies have established a direct relationship between E. coli growth rate and cellular ribosomal content for fast-proliferating cells. In this study, we integrated experimental findings with a systemic coarse-grained proteome allocation model, to characterize the physiological outcomes at slow growth rate during anaerobic fermentative catabolism of different glycolytic and non-glycolytic substrates.</p><p><strong>Results: </strong>The anaerobic catabolism of substrates favored high ribosomal abundances at lower growth rates. Interestingly, a modification of the previously discussed \"growth law\", the ratio of active to inactive ribosomal proteome was found to be linearly related to the growth rate for cells proliferating at slow to moderate regime (growth rate < 0.8 h^- 1). Also, under nutrient- and oxygen-limiting growth conditions, the proteome proportion allocated for ribosomal activity was reduced, and the resources were channelized towards metabolic activities to overcome the limitations imposed during uptake and metabolizing substrate. The energy-intensive uptake mechanism or lower substrate affinity, expended more catabolic proteome, which reduced its availability to other cellular functions.</p><p><strong>Conclusions: </strong>Thus, the nature of catabolic substrates imposed either uptake limitation or metabolic limitation coupled with ribosomal limitation (arising due to anoxic and nutritional stress), which resulted in higher proteome expenditure leading to sub-optimal growth phenotype. This study can form the basis for analyzing E. coli's ability to optimize metabolic efficiency under different environmental conditions- including stress responses. It can be further extended to optimizing the industrial anaerobic conversions for improving productivity and yield.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"71"},"PeriodicalIF":4.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11938722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, expression, and in silico profiling of a thermostable xylanase from Geobacillus stearothermophilus strain NASA267: insights into structural features and agro-waste valorization.","authors":"Safaa M Ali, Nehad Noby, Nadia A Soliman, Sanaa H Omar","doi":"10.1186/s12934-025-02672-6","DOIUrl":"10.1186/s12934-025-02672-6","url":null,"abstract":"<p><strong>Background: </strong>Xylanase is an industrial enzyme with diverse applications, including nutritional supplements, agro-waste valorization, and paper pulp bleaching. This study aims to investigate the production of recombinant thermostable xylanase for converting plant biomass into fermentable sugars, a key step in various industrial processes.</p><p><strong>Results: </strong>Geobacillus stearothermophilus strain NASA267, a Gram-positive, thermophilic bacterium, was identified as the top xylanase producer from samples collected in Egypt and Saudi Arabia. The xylanase gene xyl267 was successfully cloned from the NASA267 strain and heterologously expressed in E. coli under the control of a Lambda promoter. Optimal expression conditions were determined, with the highest enzyme activity (40 U/ml) achieved after 4 h of induction at 42 ℃. SDS-PAGE analysis revealed that the molecular weight of the recombinant xylanase was approximately 40 kDa, consistent with the calculated molecular weight (38.6 kDa) based on its amino acid sequence (331 aa). Enzyme sequence and structural analysis revealed that xyl267 shows typical TIM barrel fold where Glu134 and Glu241 constitute the enzyme active site. The xyl267 demonstrated optimal activity at 65 ℃ and maintained full stability up to 60 ℃, while it displayed a half-life of 8 min at 80 ℃. It remained stable at - 20 ℃ for up to 50 days and was most active at pH 8. Although the enzyme was active in presence of various salts, solvents, and cations, the exposure to Cu²⁺, Zn²⁺, Mn²⁺, and methanol reduced the enzyme activity by 47%, 37%, 31%, and 8%, respectively. The enzyme was effective in saccharifying agro-waste, particularly pretreated banana peel, which produced the highest sugar content. These findings highlight xyl267s potential for biomass conversion and industrial applications in high-temperature and alkaline environment.</p><p><strong>Conclusion: </strong>The xyl267 from a NASA strain was cloned and successfully overexpressed in E. coli, producing a ~ 40 kDa recombinant enzyme. It showed optimal activity at 65 ℃, and was most active at pH 8. While it retained activity in various salts and solvents, it was inhibited by some heavy metals. Xyl267 effectively released fermentable sugars from pretreated banana peel, making it a promising candidate for industrial applications in high-temperature, alkaline environments and agro-waste saccharification.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"69"},"PeriodicalIF":4.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial biosynthesis of abietane-type diterpene ferruginol from glucose.","authors":"Hyun Jeong Lee, Chaeyeon Kim, Yu Been Heo, Seong-Eun Kim, Han Min Woo","doi":"10.1186/s12934-025-02691-3","DOIUrl":"10.1186/s12934-025-02691-3","url":null,"abstract":"<p><strong>Background: </strong>Microbial supply of plant extracts is a promising biomanufacturing strategy that requires engineering of metabolic pathways and enzymes. This study presents the engineering of Corynebacterium glutamicum for heterologous production of diterpenes miltiradiene and ferruginol.</p><p><strong>Results: </strong>Through targeted metabolic pathway modifications, including inactivation of pyruvate carboxylase and phytoene synthase, the HL01 strain was optimized to enhance pyruvate and geranylgeranyl pyrophosphate (GGPP) pools. Overexpression of key MEP pathway enzymes (Dxs and Idi) and implementation of three GGPP synthase modules further boosted diterpene synthesis. Then, combining those modules with diterpene synthase (DiTPS) and intact P450 reductase modules (CYP76AH1 and CPR1) enabled production of miltiradiene (ferruginol equivalent) at 237.46 ± 34.8 mg/L and ferruginol at 107.34 ± 1.2 mg/L under constant glucose feeding, respectively.</p><p><strong>Conclusions: </strong>Modular gene expression for heterologous metabolic pathway can be optimized for bacterial biosynthesis. This is the first demonstration of ferruginol production in bacteria. These findings pave the way for further optimization of diterpene biosynthesis through pathway engineering and module integration in bacterial systems.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"67"},"PeriodicalIF":4.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gluconobacter oxydans DSM 50049 - an efficient biocatalyst for oxidation of 5-formyl-2-furancarboxylic acid (FFCA) to 2,5-furandicarboxylic acid (FDCA).","authors":"Mahmoud Sayed, Mohamed Ismail, Anirudh Sivasubramanian, Riko Kawano, Chengsi Li, Sara Jonsdottir Glaser, Rajni Hatti-Kaul","doi":"10.1186/s12934-025-02689-x","DOIUrl":"10.1186/s12934-025-02689-x","url":null,"abstract":"<p><strong>Background: </strong>2,5-Furandicarboxylic acid (FDCA) is a promising building block for biobased recyclable polymers and a platform for other potential biobased chemicals. The common route of its production is by oxidation of sugar-derived 5-hydroxymethylfurfural (HMF). Several reports on biocatalytic oxidation using whole microbial cells or enzymes have been reported, which offers potentially a greener alternative compared to the chemical process. HMF oxidases and aryl alcohol oxidases are the only enzymes able to catalyse the complete oxidation to FDCA, however at low concentrations and are subject to inhibition by the FFCA (5-formylfuran-2-carboxylic acid) intermediate. The present report presents a study on the oxidation of FFCA to FDCA using the obligately aerobic bacterium Gluconobacter oxydans and identification of the enzymes catalyzing the reaction.</p><p><strong>Results: </strong>Screening of three different strains showed G. oxydans DSM 50049 to possess the highest FFCA oxidation efficiency. Optimal reaction conditions for obtaining 100% conversion of 10 g/L (71 mM) FFCA to FDCA at 100% reaction yield were at pH 5, 30 °C and using 200 mg wwt /mL cells harvested at mild-exponential phase. In a reaction run at a 1 L scale using a total of 15 g/L (107 mM) FFCA supplied in a fed-batch mode, FDCA was obtained at a yield of 90% in 8.5 h. The product was recovered at 82% overall yield and 99% purity using a simple recovery process. Screening of several oxidoreductase enzymes from the gene sequences identified in the bacterial genome revealed two proteins annotated as membrane-bound aldehyde dehydrogenase (MALDH) and coniferyl aldehyde dehydrogenase (CALDH) to be the enzymes catalyzing the oxidization of FFCA.</p><p><strong>Conclusion: </strong>The study shows G. oxydans DSM 50049 and its enzymes to be promising biocatalysts for use in the FDCA production process from biomass. The high reaction rate and yield motivate further studies on characterization of the identified enzymes exhibiting the FFCA oxidizing activity, which can be used to construct an enzyme cascade together e.g. with HMF oxidase or aryl alcohol oxidase for one-pot production of FDCA from 5-HMF.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"68"},"PeriodicalIF":4.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an efficient expression system for human chaperone BiP in Pichia pastoris: production optimization and functional validation.","authors":"Eimantas Žitkus, Evaldas Čiplys, Mantas Žiaunys, Andrius Sakalauskas, Rimantas Slibinskas","doi":"10.1186/s12934-025-02679-z","DOIUrl":"10.1186/s12934-025-02679-z","url":null,"abstract":"<p><strong>Background: </strong>Human BiP, or GRP78, is a molecular chaperone mainly found in the endoplasmic reticulum (ER). However, a growing amount of data also associates BiP with many distinct functions in subcellular locations outside the ER. Notably, several diseases have been BiP-related, so the protein could potentially be used for therapeutic purposes. This study aimed to optimize a high cell-density fermentation process for the production of recombinant human BiP (rhBiP) in yeast Pichia pastoris in a mineral medium.</p><p><strong>Results: </strong>P. pastoris cells successfully synthesized and secreted full-length rhBiP protein in a complex growth medium. However, secreted rhBiP titer was considerably lower when P. pastoris was cultivated in a defined mineral basal salt medium (BSM). During rhBiP synthesis optimization in shake flasks, it was found that the addition of reducing compounds (DTT or TCEP) to mineral BSM medium is essential for high-yield rhBiP production. Furthermore, rhBiP secretion in the BSM medium was significantly increased by feeding yeast with an additional carbon source. The addition of 2 mM DTT and 0.5-1.0% of glucose/glycerol to the BSM medium increased rhBiP titer ~ 8 times in the shake flasks. Glucose/methanol mixture feeding with added 2 mM DTT before induction was applied in high-density P. pastoris fermentation in bioreactor. Oxygen-limited fermentation strategy allowed to achieve ~ 70 mg/L rhBiP in BSM medium. Hydrophobic interaction and anion exchange chromatography were used for rhBiP protein purification. Approximately 45 mg rhBiP was purified from 1 L growth medium, and according to SDS-PAGE, ~ 90% purity was reached. According to data presented in this study, rhBiP protein derived from P. pastoris is a full-length polypeptide that has ATPase activity. In addition, we show that P. pastoris-derived rhBiP effectively inhibits neurodegenerative disease-related amyloid beta 1-42 (Aβ₄₂) peptide and alpha-synuclein (α-Syn) protein aggregation in vitro.</p><p><strong>Conclusions: </strong>A scalable bioprocess to produce rhBiP in P. pastoris was developed, providing a high yield of biologically active protein in a chemically defined mineral medium. It opens a source of rhBiP to accelerate further therapeutic applications of this important protein.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"66"},"PeriodicalIF":4.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11917157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetol biosynthesis enables NADPH balance during nitrogen limitation in engineered Escherichia coli.","authors":"Suresh Sudarsan, Philipp Demling, Emre Ozdemir, Aziz Ben Ammar, Philip Mennicken, Joerg M Buescher, Guido Meurer, Birgitta E Ebert, Lars M Blank","doi":"10.1186/s12934-025-02687-z","DOIUrl":"10.1186/s12934-025-02687-z","url":null,"abstract":"<p><strong>Background: </strong>Nutrient limitation strategies are commonly applied in bioprocess development to engineered microorganisms to further maximize the production of the target molecule towards theoretical limits. Biomass formation is often limited under the limitation of key nutrients, and understanding how fluxes in central carbon metabolism are re-routed during the transition from nutrient excess to nutrient-limited condition is vital to target and tailor metabolic engineering strategies. Here, we report the physiology and intracellular flux distribution of an engineered acetol-producing Escherichia coli on glycerol under nitrogen-limited, non-growing production conditions.</p><p><strong>Results: </strong>Acetol production in the engineered E. coli strain is triggered upon nitrogen depletion. During nitrogen limitation, glycerol uptake decreased, and biomass formation rates ceased. We applied ¹³C-flux analysis with 2-¹³C glycerol during exponential growth and nitrogen starvation to elucidate flux re-routing in the central carbon metabolism. The results indicate a metabolically active non-growing state with significant flux re-routing towards acetol biosynthesis and reduced flux through the central carbon metabolism. The acetol biosynthesis pathway is favorable for maintaining the NADPH/NADP⁺ balance.</p><p><strong>Conclusion: </strong>The results reported in this study illustrate how the production of a value-added chemical from a waste stream can be connected to the metabolism of the whole-cell biocatalyst, making product formation mandatory for the cell to maintain its NADPH/NADP⁺ balance. This has implications for process design and further metabolic engineering of the whole-cell biocatalyst.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"65"},"PeriodicalIF":4.3,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The comparison of gut microbiota between different types of epilepsy in children.","authors":"Siwei Fang, Nanfei Hu, Changci Zhou, Jiajia You, Liwen Wu, Xiongfeng Pan, Zhenghui Xiao, Jun Qiu","doi":"10.1186/s12934-025-02684-2","DOIUrl":"10.1186/s12934-025-02684-2","url":null,"abstract":"<p><strong>Objective: </strong>To better understand the variations in gut microbiota in children with different types of epilepsy.</p><p><strong>Methods: </strong>Thirty-seven children with epilepsy were included in the case group, which was further divided into focal (group A, n = 28) and generalized epilepsy groups (group B, n = 9) based on the origin and extent of the seizures. The focal epilepsy group was subdivided into the benign childhood epilepsy with centrotemporal spikes (BECT) (group C, n = 9) and non-BECT groups (group D, n = 19) based on the appearance of typical centrotemporal spikes or spike-wave complexes on the electroencephalogram (EEG). Additionally, 14 healthy children were selected as the control group (group E, n = 14).</p><p><strong>Results: </strong>Significant differences were observed in the diversity and composition of gut microbiota between the case and control groups. At the genus level, the abundance of Megamonas (P = 0.001), Streptococcus (P<0.001), Romboutsia (P = 0.001), Bacteroides (P<0.05), and Escherichia/Shigella (P<0.05) was significantly higher in the focal epilepsy group than in the control group (0.027 vs. 0.00009, P = 0.001; 0.016 vs. 0.002, P<0.001; 0.013 vs. 0.002, P = 0.001; 0.030 vs. 0.002, P<0.05, respectively). Additionally, Escherichia/Shigella (P<0.05) was more abundant in the case group compared to the control group (0.033 vs. 0.002, P<0.05). Bacteroides (P<0.05) was more abundant in the control group than in the case group. Megamonas (P<0.001) and Collinsella (P<0.05) were significantly more prevalent in the BECT group than in the control group (0.034 vs. 0.00009, P<0.001; 0.014 vs. 0.001, P<0.05, respectively). In the non-BECT group, compared to the control group, Megamonas (P = 0.013), Streptococcus (P<0.001), Romboutsia (P = 0.001), and Escherichia/Shigella (P<0.05) were found in greater abundance (0.023 vs. 0.00009, P = 0.013; 0.018 vs. 0.002, P<0.001; 0.014 vs. 0.002, P = 0.001; 0.037 vs. 0.002, P<0.05, respectively).</p><p><strong>Conclusions: </strong>Though, there were no statistically significant differences in gut microbiota between the different types of epilepsy, the gut microbiota of children with epilepsy significantly differed from that of healthy controls. The increased abundance of Escherichia/Shigella may lead to the worsening of clinical phenotypes and poor prognosis, and it could be a candidate biomarker to identify the focal epilepsy or even non-benign childhood epilepsy with centrotemporal spikes, potentially providing new therapeutic targets for the future.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"64"},"PeriodicalIF":4.3,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioremediation of non-point hydrogen sulfide emissions using bacterial cellulose/activated carbon membrane.","authors":"Mingbo Yang, Yan Zhang, Xueqing Zhao, Ge Gao, Yucheng Shi, Yifan Wang, Mengyue Duan, Ziye Guo, Xiaodong Ma, Ting Ma, Guoqiang Li","doi":"10.1186/s12934-025-02686-0","DOIUrl":"10.1186/s12934-025-02686-0","url":null,"abstract":"<p><strong>Background: </strong>Hydrogen sulfide (H₂S) gas, characterized by its low odor threshold and toxicity, poses significant challenges in non-point source odor management. Traditional biotechnologies are effective in removing malodorous gases from point sources but they are limited for non-point source odor control.</p><p><strong>Results: </strong>In this study, the sqr and pdo genes from Cupriavidus pinatubonensis JMP134 were introduced into the bacterial cellulose-producing strain Kosakonia oryzendophytica FY-07. This genetic modification enhanced the strain's sulfur oxidation capacity, which increased over time, with an average transformation capacity of approximately 275 mg·L^- 1·day^- 1. By incorporating 1% activated carbon, an efficient, naturally degradable bio-composite membrane was developed, achieving a maximum H₂S adsorption capacity of 7.3 g·m^- 3·day^- 1. FY-07 remained stable in soil and improved the microbial community for H₂S treatment.</p><p><strong>Conclusion: </strong>The resulting bio-composite membrane is environment-friendly and efficient, making it suitable for emergency odor control in landfills. This study offers recommendations for using membrane materials in managing non-point hydrogen sulfide emissions.</p>","PeriodicalId":18582,"journal":{"name":"Microbial Cell Factories","volume":"24 1","pages":"63"},"PeriodicalIF":4.3,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11899930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}