Microbiology最新文献_第5页

Isolation, Identification, and Survival Strategy of the Halotolerant Strain Dietzia maris MX2 from the Yakshinskoe Mineral Salts Deposit 雅克辛斯科夫矿物盐矿藏中耐盐碱菌株 Dietzia maris MX2 的分离、鉴定和生存策略

IF 1.5 4区生物学

Microbiology Pub Date : 2024-03-22 DOI: 10.1134/s0026261723602919

M. A. Kharitonova, F. G. Kupriyanova-Ashina, T. R. Shakirov, M. S. Vafina, O. N. Ilinskaya

{"title":"Isolation, Identification, and Survival Strategy of the Halotolerant Strain Dietzia maris MX2 from the Yakshinskoe Mineral Salts Deposit","authors":"M. A. Kharitonova, F. G. Kupriyanova-Ashina, T. R. Shakirov, M. S. Vafina, O. N. Ilinskaya","doi":"10.1134/s0026261723602919","DOIUrl":"https://doi.org/10.1134/s0026261723602919","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Halophilic and halotolerant microorganisms have a high biotechnological potential. They are producers of biologically active substances, stress-protective agents, hydrolytic enzymes, and are used for environmental bioremediation. At the same time, the characterization of novel halotolerant bacteria and determination of their salt tolerance strategies are important basic problems. The present work reports the isolation of a new strain, MX2, from the salt well brine of the Yakshinskoe potassium−magnesium salt deposit. The isolate was an aerobic, gram-positive, nonmotile bacterium that did not form spores. The cell morphology varied from cocci to short rods capable of producing V-shaped forms. The colonies on agar were circular, with an entire edge and raised center, glistening and orange. Bacteria of strain MX2 were halotolerant microorganisms capable of growing at NaCl concentrations up to 9%. The genome of strain MX2 was sequenced. Its estimated size was 3 747 717 bp, and the number of protein-coding genes was 3562. Strain MX2 was identified as belonging to the species Dietzia maris based on analysis of the 16S rRNA, gyrB, rpoB, recA, and ppk gene sequences and using time-of-flight mass spectrometry (MALDI-TOF-MS). D. maris MX2 had complete metabolic pathways for the synthesis of ectoine, hydroxyectoine, and trehalose, as well as the transport systems for ectoine, hydroxyectoine, trehalose, glycerol, glycerol-3-phosphate, L-proline, and glycine-betaine. Thus, to ensure the osmotic balance, D. maris MX2 used the strategy of accumulating compatible organic solutes.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":"24 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140203359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Hydrocarbon Pollution on the Fungal Communities of the Littoral Sediments of the White and Barents Seas 碳氢化合物污染对白海和巴伦支海沿岸沉积物真菌群落的影响

IF 1.5 4区生物学

Microbiology Pub Date : 2024-03-22 DOI: 10.1134/s002626172360324x

A. Yu. Fadeev, L. A. Gavirova, M. L. Georgieva, V. V. Kozlovsky, U. V. Simakova, A. I. Shestakov, E. N. Bubnova

{"title":"Effect of Hydrocarbon Pollution on the Fungal Communities of the Littoral Sediments of the White and Barents Seas","authors":"A. Yu. Fadeev, L. A. Gavirova, M. L. Georgieva, V. V. Kozlovsky, U. V. Simakova, A. I. Shestakov, E. N. Bubnova","doi":"10.1134/s002626172360324x","DOIUrl":"https://doi.org/10.1134/s002626172360324x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The effect of hydrocarbon pollution on the fungal communities of littoral sediments of the cold-water White and Barents seas was investigated. The samples were collected at locations with different levels of pollution with oil products, from ports to relatively undisturbed areas. Using the diesel fuel-containing medium resulted in detection of hydrocarbon-degrading fungi in almost all studied samples, although in all cases they were less diverse than sugar-degrading fungi. In this relatively small group, Penicillium chrysogenum and Penicillium brevicompactum were the most common organisms. Fungal communities isolated on a sugar-containing medium exhibited higher diversity and abundance, with being the most common sugar degraders. The major factors affecting the structure of the fungal communities were the percentage of hydrocarbons in the total mass of organic carbon in the samples in the case of hydrocarbon-degrading fungi and location, for sugar degraders. In the experiment, the highest hydrocarbon-degrading activity was shown for Penicillium chrysogenum (the loss of residual hydrocarbons was 77.4%), Cadophora fastigiata (72%), and Tolypocladium inflatum (67.2%).","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":"8 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140203440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell Wall Galactofuranans and Galactomannan as Chemotaxonomic Characteristics of the Clavibacter Genus and its Species 细胞壁半乳糖呋喃和半乳甘露聚糖作为克拉维氏菌属及其种的化学分类学特征

IF 1.5 4区生物学

Microbiology Pub Date : 2024-03-22 DOI: 10.1134/s0026261723603007

E. M. Tul’skaya, D. Kim, N. V. Potekhina, A. S. Shashkov, S. N. Senchenkova, L. V. Dorofeeva, L. I. Evtushenko

{"title":"Cell Wall Galactofuranans and Galactomannan as Chemotaxonomic Characteristics of the Clavibacter Genus and its Species","authors":"E. M. Tul’skaya, D. Kim, N. V. Potekhina, A. S. Shashkov, S. N. Senchenkova, L. V. Dorofeeva, L. I. Evtushenko","doi":"10.1134/s0026261723603007","DOIUrl":"https://doi.org/10.1134/s0026261723603007","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000Abstract\u0000</h3>Two types of cell wall glycopolymers of different structure were found in the type strains of two species of phytopathogenic bacteria, Clavibacter insidiosus VKM Ac-1402T and Clavibacter nebraskensis VKM Ac-1404T (family Microbacteriaceae, class Actinomycetes). The first type was represented by new, previously undescribed (1→6)-linked β-D-galactofuranans, which in the studied strains differed in the structure of side oligosaccharide chains (the structures are given in the text). The structure of the second glycopolymer, a pyruvate-containing galactomannan, was identical in both strains. The results obtained in this work, together with those published previously, indicate that the presence of the pyruvate-containing galactomannan and galactofuranans (with an identical core structure and different side oligosaccharide substituents) can be considered as a chemotaxonomic trait of the genus Clavibacter, and galactofuranans with di-, tri-, or tetrasaccharide substituents of different composition and structures can serve as chemotaxonomic markers of the species. The data obtained expand our understanding of the structural diversity of natural glycopolymers and structural features of the bacterial cell walls in various taxa and may be of interest for taxonomic studies and the studies aimed at elucidating the molecular mechanisms of interaction between bacteria and plant cells.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":"293 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140203441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ecological Diversity of Micromycetes in Aerial Environments of Russian Libraries 俄罗斯图书馆空中环境中小霉菌的生态多样性

IF 1.5 4区生物学

Microbiology Pub Date : 2024-03-22 DOI: 10.1134/s0026261723603287

T. D. Velikova, E. A. Popikhina, E. S. Trepova, S. S. Khazova

{"title":"Ecological Diversity of Micromycetes in Aerial Environments of Russian Libraries","authors":"T. D. Velikova, E. A. Popikhina, E. S. Trepova, S. S. Khazova","doi":"10.1134/s0026261723603287","DOIUrl":"https://doi.org/10.1134/s0026261723603287","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The structure of micromycete communities in the library aerial environment was investigated in 57 Russian cities located in seven federal districts (Northwestern, Central, Southern, Volga, Ural, Siberian, and Far Eastern). A total of 107 micromycete species belonging to 41 genera were isolated and identified. Due to mostly similar conditions, the aerial ecosystem of internal spaces of the library storage facilities was relatively stable, with moderate diversity and high evenness, as was confirmed by the relevant indices: the Shannon index varied from 2.7 to 3.4, the McIntosh diversity index, from 22.7 to 140.8, and the Menhinick index, from 2.1 to 3.0. The Simpson’s dominance index and the Berger–Parker index did not exceed 0.11 and 0.24, respectively. The McIntosh and Pielou’s evenness indices were 0.71–0.78 and 0.79–0.85, respectively. High similarity of the taxonomic structures, independent of climatic conditions of the studied regions, was revealed, as was confirmed by the values of the Stugren–Radulescu and Morisita–Horn coefficients: 0.08‒0.77 and 0.04‒0.47, respectively. The typical members of the studied mycobiota were Aspergillus versicolor (7.5‒14.3), Cladosporium cladosporioides (17.5‒40.5), C. herbarum (0.8‒53.6), and Penicillium aurantiogriseum (6.5‒32.4). Most other species were scarce, with frequencies of occurrence not exceeding 7.1%.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":"364 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140203373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitin Degradation by Microbial Communities of the Kandalaksha Bay, White Sea 白海干达拉沙湾微生物群落的甲壳素降解作用

IF 1.5 4区生物学

Microbiology Pub Date : 2024-03-22 DOI: 10.1134/s0026261723603111

A. M. Dukat, A. M. Kuznetsova, S. D. Klyagin, V. O. Trushin, A. A. Klyukina, A. G. El’cheninov, I. V. Danilova

引用次数: 0

Effect of Knockout of the PPN1 Polyphosphatase and VTC4 Polyphosphate Synthetase Genes on Saccharomyces cerevisiae Growth on Ethanol and Its Mitochondrial Polyphosphates 敲除 PPN1 多磷酸酶和 VTC4 多磷酸合成酶基因对酿酒酵母在乙醇及其线粒体多磷酸盐上生长的影响

IF 1.5 4区生物学

Microbiology Pub Date : 2024-03-22 DOI: 10.1134/s0026261723603299

A. A. Tomashevski, T. V. Kulakovskaya

{"title":"Effect of Knockout of the PPN1 Polyphosphatase and VTC4 Polyphosphate Synthetase Genes on Saccharomyces cerevisiae Growth on Ethanol and Its Mitochondrial Polyphosphates","authors":"A. A. Tomashevski, T. V. Kulakovskaya","doi":"10.1134/s0026261723603299","DOIUrl":"https://doi.org/10.1134/s0026261723603299","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>One of the functions of inorganic polyphosphates (polyP) as compounds with phosphoester bonds is participation in energy metabolism. While yeast mitochondria contain their own pool of polyphosphates, the ways in which these polymers are involved in the functioning of mitochondria in these microorganisms are not well understood. The goal of the present work was to identify the effect of knockout mutations of the VTC4 gene of polyphosphate synthetase and the PPN1 gene of one of the polyphosphatases on the content of polyphosphates and polyphosphatase activity in mitochondria of S. cerevisiae and on the growth characteristics of the mutant strains on ethanol. Knockout of the VTC4 gene was shown to result in a significant decrease in the polyP content in the mitochondria. Knockout of the PPN1 gene led to the disappearance of polyphosphatase activity, but only to a slight increase in the content of mitochondrial polyphosphates during growth on glucose. When grown on ethanol, the polyP content in the mitochondria of this strain was similar to that of the parental strain, and in both strains it was approximately two times less than when grown on glucose. Both mutants were able to grow with ethanol as the carbon source; however, they exhibited a longer lag phase upon transition from glucose consumption to ethanol consumption. It was suggested that mitochondrial polyphosphates may represent the energy reserve of these organelles, which is necessary for the formation of full-fledged mitochondria during transition from glycolysis to oxidative phosphorylation.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":"18 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140203358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immobilization of Azospirillum Bacteria on Various Carriers 将 Azospirillum 细菌固定在各种载体上

IF 1.5 4区生物学

Microbiology Pub Date : 2024-03-22 DOI: 10.1134/s0026261723603123

M. A. Kupryashina, E. G. Ponomareva, T. E. Pylaev

引用次数: 0

Green Approach: Characterization, Antibacterial, Antitumor and Detoxification Potentials of Exopolysaccharides-Mediated Silver Nanoparticles Produced by Penicillium citrinum AUMC 11627 绿色方法：柠檬青霉 AUMC 11627 产生的外多糖介导的银纳米粒子的特性、抗菌、抗肿瘤和解毒潜力

IF 1.5 4区生物学

Microbiology Pub Date : 2024-03-22 DOI: 10.1134/s0026261723601525

M. M. Housseiny, E. M. Fawzy, M. A. Abu-Tahon, W. E. Abdallah, O. M. El-Mahdy

{"title":"Green Approach: Characterization, Antibacterial, Antitumor and Detoxification Potentials of Exopolysaccharides-Mediated Silver Nanoparticles Produced by Penicillium citrinum AUMC 11627","authors":"M. M. Housseiny, E. M. Fawzy, M. A. Abu-Tahon, W. E. Abdallah, O. M. El-Mahdy","doi":"10.1134/s0026261723601525","DOIUrl":"https://doi.org/10.1134/s0026261723601525","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Developing a green effective approach and a suitable applicative process for increasing the efficiency of microbial nanomaterial synthesis is an emerging prospect for future industrial production. This was accomplished by employing exopolysaccharides (EPS) to obtain potent silver nanoparticles using Penicillium citrinum (EPS−AgNPs) as a reducing and stabilizing agent. EPS−AgNPs were analyzed by UV–Vis spectrophotometry, which showed a peak of absorbance at 420 nm. TEM was used to determine the morphology of the EPS−AgNPs and showed that the particles were 14.4 nm in size, well dispersed, and spherical. The stabilization of (EPS−AgNPs) was caused by amides and amines groups, which were confirmed via Fourier transform infrared. Moreover, the EPS−AgNPs showed excellent antibacterial activities against various human pathogenic bacterial strains in comparison to the potent antibiotics. Additionally, EPS−AgNPs were examined for their anticancer properties. Furthermore, EPS−AgNPs had a significant impact on the ochratoxin production. Finally, our findings confirm the benefits of adopting a bio green technique for the synthesis of antibacterial and detoxifying nanoparticles, which are predicted to provide new pathways for various cancers and infectious microbial diseases treatment.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":"294 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140203371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-Abundance Sulfidogenic Bacteria Carry out Intensive Sulfate Reduction in Terrestrial Hydrotherms of the Barguzin Valley 低丰度硫酸化细菌在巴尔古津河谷陆地水圈中进行密集的硫酸盐还原作用

IF 1.5 4区生物学

Microbiology Pub Date : 2024-02-07 DOI: 10.1134/s0026261723603603

I. I. Rusanov, V. V. Kadnikov, A. P. Lukina, E. V. Danilova, L. O. Sokolyanskaya, N. V. Ravin, O. V. Karnachuk

引用次数: 0

Genetic Screening using High-Throughput Barcode Sequencing of Saccharomyces cerevisiae Knockout Collection: Search for Regulators of mtDNA Intracellular Selection 利用高通量条形码测序对酿酒酵母剔除集进行遗传筛选：寻找mtDNA胞内选择的调控因子

IF 1.5 4区生物学

Microbiology Pub Date : 2024-02-07 DOI: 10.1134/s0026261723604001

A. A. Burlaka, E. S. Glagoleva, N. D. Kashko, D. A. Knorre

引用次数: 0