Microbiology最新文献_第10页

Effect of Temperature on the Protective Properties of the Escherichia coli DNA-Binding Protein Dps under Desiccation Stress 温度对干燥胁迫下大肠杆菌 DNA 结合蛋白 Dps 保护特性的影响

IF 1.5 4区生物学

Microbiology Pub Date : 2024-02-07 DOI: 10.1134/s0026261723603640

N. G. Loiko, E. V. Tereshkin, V. V. Kovalenko, Y. F. Krupyanskii, K. B. Tereshkina

引用次数: 0

Transcriptome Analysis of a Wine Yeast Strain with a Deletion of the CAR1 Arginase Gene 缺失 CAR1 精氨酸酶基因的葡萄酒酵母菌株的转录组分析

IF 1.5 4区生物学

Microbiology Pub Date : 2024-02-07 DOI: 10.1134/s0026261723603901

A. V. Mardanov, E. A. Vasyagin, E. S. Mardanova, A. V. Beletsky, M. Yu. Shalamitskiy, T. N. Tanashchuk, V. N. Urakov, V. V. Kushnirov, A. L. Rakitin, N. V. Ravin

{"title":"Transcriptome Analysis of a Wine Yeast Strain with a Deletion of the CAR1 Arginase Gene","authors":"A. V. Mardanov, E. A. Vasyagin, E. S. Mardanova, A. V. Beletsky, M. Yu. Shalamitskiy, T. N. Tanashchuk, V. N. Urakov, V. V. Kushnirov, A. L. Rakitin, N. V. Ravin","doi":"10.1134/s0026261723603901","DOIUrl":"https://doi.org/10.1134/s0026261723603901","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000Abstract—</h3>Ethyl carbamate, a potential carcinogen, is formed in wine as a result of a chemical reaction between ethanol and urea during fermentation and storage. Using genome editing methods, a modified strain of wine yeast Saccharomyces cerevisiae was constructed, with a deletion of the CAR1 arginase gene, leading to a decrease in the formation of urea from arginine. Transcriptome analysis by high-throughput sequencing identified 738 genes whose transcription level reliably changed more than twofold as a result of deletion of the CAR1 gene. Expression of most genes of the central metabolic pathways in the CAR1 deletion strain was decreased; increased transcription levels were observed for the Map kinase signaling cascades. Among the genes of arginine metabolism pathways, the level of transcription of the ornithine aminotransferase gene CAR2 increased significantly. The wine material obtained using a strain with a deletion of the CAR1 gene under micro-winemaking conditions contained 25% less urea than in the case of using an unmodified strain. The resulting strain of wine yeast, having lower urea levels, may be promising for winemaking.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139761271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic Characterization and Genome Analysis of a Novel Isolate of the Spiral-Shaped Methanotroph “Methylospira mobilis” 螺旋形甲烷菌 "Methylospira mobilis "新菌株的表型特征和基因组分析

IF 1.5 4区生物学

Microbiology Pub Date : 2024-02-07 DOI: 10.1134/s0026261723603627

O. V. Danilova, I. Y. Oshkin, R. S. Suleimanov, S. N. Dedysh

{"title":"Phenotypic Characterization and Genome Analysis of a Novel Isolate of the Spiral-Shaped Methanotroph “Methylospira mobilis”","authors":"O. V. Danilova, I. Y. Oshkin, R. S. Suleimanov, S. N. Dedysh","doi":"10.1134/s0026261723603627","DOIUrl":"https://doi.org/10.1134/s0026261723603627","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Taxon “Methylospira mobilis” accommodates micro-aerobic, spiral-shaped methanotrophic bacteria, which are widely distributed in low-oxygen habitats. Due to difficulties of cultivation, these methanotrophs remained represented by a single isolate, strain Shm1, which was obtained from a boreal peat bog of European Russia. This study reports isolation of a novel member of this candidate genus, strain SPMX, obtained from a subarctic wetland. The 16S rRNA gene sequence of strain SPMX was identical to that in strain Shm1. The complete genome sequence of strain SPMX was 4.6 Mb in size and displayed 99.5% average nucleotide identity to the genome sequence of strain Shm1, thus suggesting affiliation of these bacteria to one methanotroph species. In contrast to strain Shm1, the new isolate was more tolerant to low temperatures and was less growth-dependent on the presence of СО2. The genomes of both spiral-shaped methanotrophs encoded form II ribulose bisphosphate carboxylase, which makes them different from the majority of other type X methanotrophs that possess form I of this enzyme.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140884149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methylocapsa polymorpha sp. nov., a Novel Dinitrogen-Fixing Methanotroph from a Subarctic Wetland 来自亚北极湿地的新型固氮甲烷菌 Methylocapsa polymorpha sp.

IF 1.5 4区生物学

Microbiology Pub Date : 2024-02-07 DOI: 10.1134/s0026261723603949

S. E. Belova, I. Y. Oshkin, K. K. Miroshnikov, N. E. Suzina, O. V. Danilova, S. N. Dedysh

{"title":"Methylocapsa polymorpha sp. nov., a Novel Dinitrogen-Fixing Methanotroph from a Subarctic Wetland","authors":"S. E. Belova, I. Y. Oshkin, K. K. Miroshnikov, N. E. Suzina, O. V. Danilova, S. N. Dedysh","doi":"10.1134/s0026261723603949","DOIUrl":"https://doi.org/10.1134/s0026261723603949","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>A novel isolate of aerobic, mildly acidophilic methanotrophic bacteria, strain RX1T, was obtained from a subarctic Sphagnum peat bog in European Russia. Strain RX1T was represented by highly polymorphic, gram-negative, non-motile, encapsulated cells that possessed a particulate methane monooxygenase enzyme and grew on methane, methanol and acetate. Electron microscopy of ultrathin cell sections revealed stacks of intracytoplasmic membrane vesicles located on one side of a cell as characteristic for methanotrophs of the genus Methylocapsa. Strain RX1T was capable of fixing dinitrogen, belonged to the family Beijerinckiaceae, and was most closely related to the obligate methanotroph Methylocapsa acidiphila B2T (98.8% 16S rRNA gene sequence similarity and 92.2% of PmoA sequence identity). The genome of strain RX1T consisted of a 4.02 Mb chromosome and a 291 kb plasmid, and contained one rrn operon, one pmoCAB operon and about 3800 protein-coding genes. The average nucleotide identity between the genomes of strain RX1T and M. acidiphila B2T was 80.0%. Based on the distinct cell morphology, phenotypic and genotypic differences between strain RXT and earlier described members of the genus Methylocapsa, we propose to classify it as representing a novel species of this genus, M. polymorpha, with the type strain RX1T = VKM B-3754T = UQM 41806T.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140884000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probiotics on a Mineral Zeolite-Containing Carrier 含矿物沸石载体上的益生菌

IF 1.5 4区生物学

Microbiology Pub Date : 2024-02-07 DOI: 10.1134/s002626172360372x

O. N. Ilinskaya, A. G. Galeeva, M. A. Kharitonova, A. I. Kolpakov, M. S. Glukhov, O. N. Lopatin

引用次数: 0

Isolation of Microorganisms-Destructors from Palm Oil Production Waste and Study of Their Biotechnological Potential 从棕榈油生产废弃物中分离微生物-破坏者并研究其生物技术潜力

IF 1.5 4区生物学

Microbiology Pub Date : 2024-02-07 DOI: 10.1134/s0026261723603937

A. L. Gerasimchuk, Y. S. Topilina, A. N. Sysoeva, A. A. Trifonov, E. Nurhayati, A. Y. Bagastyo, D. A. Ivasenko

引用次数: 0

Distribution and Potential Ecophysiological Roles of Multiple GroEL Chaperonins in Pink-Pigmented Facultative Methylotrophs 多种 GroEL 合子蛋白在粉色色素兼性养甲菌中的分布和潜在生态生理作用

IF 1.5 4区生物学

Microbiology Pub Date : 2024-02-01 DOI: 10.1134/s0026261723601768

{"title":"Distribution and Potential Ecophysiological Roles of Multiple GroEL Chaperonins in Pink-Pigmented Facultative Methylotrophs","authors":"","doi":"10.1134/s0026261723601768","DOIUrl":"https://doi.org/10.1134/s0026261723601768","url":null,"abstract":" <h3>Abstract</h3> The distribution and phylogeny of the GroEL chaperonin genes in the type strains of all described species of pink-pigmented methylotrophic bacteria (PPFM) belonging to the genera Methylobacterium and Methylorubrum were analyzed. Half of the bacterial strains tested (38 out of 69) were found to possess multiple groEL genes. Analysis of their translated amino acid sequences and promoter regions preceding the groESL operons that include them demonstrated that the GroEL chaperonins of these methylotrophs form three similarity groups typical of PPFM. The largest of these (GroEL1) combines, apparently, essential housekeeping chaperonins, and the other two consist of additional separately clustered proteins that differ in the composition of the elements regulating their gene expression. The strains encoding proteins of the GroEL2 group were isolated from various environments, including those contaminated with industrially produced C1-compounds, while bacteria possessing GroEL3-like chaperonins are predominantly plant symbionts. It has been proposed that GroEL3 proteins may be involved in phytosymbiotic processes, whereas GroEL2 chaperonins can participate in response to specific stresses experienced by host cells in their habitats. At the same time, the GroEL chaperonin of Methylobacterium brachiatum B0021T, atypical for PPFM, seem to be intended for folding of dinuclear iron monooxygenase, in whose gene cluster it is encoded. Further testing of these assumptions should elucidate the roles of multiple GroEL chaperonins in PPFM and allow more complete use of their biotechnological potential as plant growth stimulants and biodegradation/bioremediation agents. ","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140203384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Psychrotolerant Pseudomonads Isolated from Organogenic Clay Deposits of the Mramornaya Cave (Primorskii Krai) 从姆拉莫尔纳亚洞穴（滨海边疆区）有机粘土沉积物中分离出的精神耐受性假单胞菌的特征

IF 1.5 4区生物学

Microbiology Pub Date : 2024-02-01 DOI: 10.1134/s0026261723603263

{"title":"Characteristics of Psychrotolerant Pseudomonads Isolated from Organogenic Clay Deposits of the Mramornaya Cave (Primorskii Krai)","authors":"","doi":"10.1134/s0026261723603263","DOIUrl":"https://doi.org/10.1134/s0026261723603263","url":null,"abstract":" <h3>Abstract</h3> The community of cultured pseudomonads isolated from clayey organogenic deposits of the Mramornaya Cave (Primorskii krai) was investigated. The bacterial strains isolated in this work were eurythermal and psychrotolerant. Their taxonomic position was determined by high-throughput sequencing of the 16S rRNA gene fragments. Members of the genus Pseudomonas are known to inhabit all the ecological niches on Earth and, accordingly, have a wide range of adaptive functions. Microscopic techniques were used to establish the changes in the character of motility and the cell size stability with changes in the cultivation temperature. The studied strains are of scientific and applied interest due to their enzymatic activity against several substrates simultaneously at different temperatures (25 and 4°C), as well as to the ability to secrete cold-active pectinase, protease, and lipase. However, phosphate-solubilizing activity both at 4 and at 25°C became preferable for the strains. The Mramornaya Cave is of karst origin and is characterized by carbonate karst, which explains the preference for calcium phosphate in the studied strains. Analysis of the obtained data showed that the collection of cultivated bacteria obtained in the present work included both typical psychrotolerant ones, which exhibited enzymatic activity at optimal growth temperature, and unique ones, capable of synthesizing a wide range of enzymes under the temperature different from their growth optimum. ","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140203368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viability and Ultrastructural Changes of Bacterial Cells Grown in the Presence of a Pollutant 存在污染物时生长的细菌细胞的活力和超微结构变化

IF 1.5 4区生物学

Microbiology Pub Date : 2023-12-01 DOI: 10.1134/s0026261723603512

{"title":"Viability and Ultrastructural Changes of Bacterial Cells Grown in the Presence of a Pollutant","authors":"","doi":"10.1134/s0026261723603512","DOIUrl":"https://doi.org/10.1134/s0026261723603512","url":null,"abstract":" <h3>Abstract</h3> Microbial degradation of pollutants is a safe and cost-effective way to clean the environment. However, little is known on changes at the cell ultrastructure level and on the mechanisms of adaptation in general to xenobiotics under conditions of their biodegradation. Rhodococcus sp. strain 7Ba is capable of preserving long-term viability and the ability to degrade when growing with phenol at up to 1 g/L. Destructive morphological changes at the ultrastructural level were not detected in the cells growing on phenol, which indicated rapid adaptation to it. Strain 7Ba cells formed a polysaccharide matrix under all experimental conditions. Investigation of the growth of spore-forming bacteria cells on glyphosate (0.5 g/L) showed that, although the substrate was not optimal, the cells were able to grow on it. The cells of Paenibacillus sp. GP5-2 and Rossellomorea sp. GP5-7 exhibited ultrastructural changes and a delay in spore formation (for strain GP5-2). The detected changes were adaptive, and further study will improve our understanding of their strategy ensuring the preservation of survival and the ability to degrade. ","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139761270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the hps/phi Copy Number on the Growth of Methylococcus capsulatus MIR hps/phi 拷贝数对荚膜甲球菌 MIR 生长的影响

IF 1.5 4区生物学

Microbiology Pub Date : 2023-12-01 DOI: 10.1134/s0026261723603536

引用次数: 0