E. M. Tul’skaya, D. Kim, N. V. Potekhina, A. S. Shashkov, S. N. Senchenkova, L. V. Dorofeeva, L. I. Evtushenko
{"title":"细胞壁半乳糖呋喃和半乳甘露聚糖作为克拉维氏菌属及其种的化学分类学特征","authors":"E. M. Tul’skaya, D. Kim, N. V. Potekhina, A. S. Shashkov, S. N. Senchenkova, L. V. Dorofeeva, L. I. Evtushenko","doi":"10.1134/s0026261723603007","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\n<b>Abstract</b>\n</h3><p>Two types of cell wall glycopolymers of different structure were found in the type strains of two species of phytopathogenic bacteria, <i>Clavibacter insidiosus</i> VKM Ac-1402<sup>T</sup> and <i>Clavibacter nebraskensis</i> VKM Ac-1404<sup>T</sup> (family <i>Microbacteriaceae</i>, class <i>Actinomycetes</i>). The first type was represented by new, previously undescribed (1→6)-linked β-D-galactofuranans, which in the studied strains differed in the structure of side oligosaccharide chains (the structures are given in the text). The structure of the second glycopolymer, a pyruvate-containing galactomannan, was identical in both strains. The results obtained in this work, together with those published previously, indicate that the presence of the pyruvate-containing galactomannan and galactofuranans (with an identical core structure and different side oligosaccharide substituents) can be considered as a chemotaxonomic trait of the genus <i>Clavibacter</i>, and galactofuranans with di-, tri-, or tetrasaccharide substituents of different composition and structures can serve as chemotaxonomic markers of the species. The data obtained expand our understanding of the structural diversity of natural glycopolymers and structural features of the bacterial cell walls in various taxa and may be of interest for taxonomic studies and the studies aimed at elucidating the molecular mechanisms of interaction between bacteria and plant cells.</p>","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":"293 1","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cell Wall Galactofuranans and Galactomannan as Chemotaxonomic Characteristics of the Clavibacter Genus and its Species\",\"authors\":\"E. M. Tul’skaya, D. Kim, N. V. Potekhina, A. S. Shashkov, S. N. Senchenkova, L. V. Dorofeeva, L. I. Evtushenko\",\"doi\":\"10.1134/s0026261723603007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">\\n<b>Abstract</b>\\n</h3><p>Two types of cell wall glycopolymers of different structure were found in the type strains of two species of phytopathogenic bacteria, <i>Clavibacter insidiosus</i> VKM Ac-1402<sup>T</sup> and <i>Clavibacter nebraskensis</i> VKM Ac-1404<sup>T</sup> (family <i>Microbacteriaceae</i>, class <i>Actinomycetes</i>). The first type was represented by new, previously undescribed (1→6)-linked β-D-galactofuranans, which in the studied strains differed in the structure of side oligosaccharide chains (the structures are given in the text). The structure of the second glycopolymer, a pyruvate-containing galactomannan, was identical in both strains. The results obtained in this work, together with those published previously, indicate that the presence of the pyruvate-containing galactomannan and galactofuranans (with an identical core structure and different side oligosaccharide substituents) can be considered as a chemotaxonomic trait of the genus <i>Clavibacter</i>, and galactofuranans with di-, tri-, or tetrasaccharide substituents of different composition and structures can serve as chemotaxonomic markers of the species. The data obtained expand our understanding of the structural diversity of natural glycopolymers and structural features of the bacterial cell walls in various taxa and may be of interest for taxonomic studies and the studies aimed at elucidating the molecular mechanisms of interaction between bacteria and plant cells.</p>\",\"PeriodicalId\":18514,\"journal\":{\"name\":\"Microbiology\",\"volume\":\"293 1\",\"pages\":\"\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-03-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1134/s0026261723603007\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0026261723603007","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Cell Wall Galactofuranans and Galactomannan as Chemotaxonomic Characteristics of the Clavibacter Genus and its Species
Abstract
Two types of cell wall glycopolymers of different structure were found in the type strains of two species of phytopathogenic bacteria, Clavibacter insidiosus VKM Ac-1402T and Clavibacter nebraskensis VKM Ac-1404T (family Microbacteriaceae, class Actinomycetes). The first type was represented by new, previously undescribed (1→6)-linked β-D-galactofuranans, which in the studied strains differed in the structure of side oligosaccharide chains (the structures are given in the text). The structure of the second glycopolymer, a pyruvate-containing galactomannan, was identical in both strains. The results obtained in this work, together with those published previously, indicate that the presence of the pyruvate-containing galactomannan and galactofuranans (with an identical core structure and different side oligosaccharide substituents) can be considered as a chemotaxonomic trait of the genus Clavibacter, and galactofuranans with di-, tri-, or tetrasaccharide substituents of different composition and structures can serve as chemotaxonomic markers of the species. The data obtained expand our understanding of the structural diversity of natural glycopolymers and structural features of the bacterial cell walls in various taxa and may be of interest for taxonomic studies and the studies aimed at elucidating the molecular mechanisms of interaction between bacteria and plant cells.
期刊介绍:
Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.