Isolation, Identification, and Survival Strategy of the Halotolerant Strain Dietzia maris MX2 from the Yakshinskoe Mineral Salts Deposit

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-03-22 DOI:10.1134/s0026261723602919

M. A. Kharitonova, F. G. Kupriyanova-Ashina, T. R. Shakirov, M. S. Vafina, O. N. Ilinskaya

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Abstract

Halophilic and halotolerant microorganisms have a high biotechnological potential. They are producers of biologically active substances, stress-protective agents, hydrolytic enzymes, and are used for environmental bioremediation. At the same time, the characterization of novel halotolerant bacteria and determination of their salt tolerance strategies are important basic problems. The present work reports the isolation of a new strain, MX2, from the salt well brine of the Yakshinskoe potassium−magnesium salt deposit. The isolate was an aerobic, gram-positive, nonmotile bacterium that did not form spores. The cell morphology varied from cocci to short rods capable of producing V-shaped forms. The colonies on agar were circular, with an entire edge and raised center, glistening and orange. Bacteria of strain MX2 were halotolerant microorganisms capable of growing at NaCl concentrations up to 9%. The genome of strain MX2 was sequenced. Its estimated size was 3 747 717 bp, and the number of protein-coding genes was 3562. Strain MX2 was identified as belonging to the species Dietzia maris based on analysis of the 16S rRNA, gyrB, rpoB, recA, and ppk gene sequences and using time-of-flight mass spectrometry (MALDI-TOF-MS). D. maris MX2 had complete metabolic pathways for the synthesis of ectoine, hydroxyectoine, and trehalose, as well as the transport systems for ectoine, hydroxyectoine, trehalose, glycerol, glycerol-3-phosphate, L-proline, and glycine-betaine. Thus, to ensure the osmotic balance, D. maris MX2 used the strategy of accumulating compatible organic solutes.

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雅克辛斯科夫矿物盐矿藏中耐盐碱菌株 Dietzia maris MX2 的分离、鉴定和生存策略

摘要嗜卤和耐卤微生物具有很高的生物技术潜力。它们能产生生物活性物质、应激保护剂和水解酶，并可用于环境生物修复。同时，新型耐盐细菌的特征描述及其耐盐策略的确定也是重要的基础问题。本研究报告了从雅克辛斯克钾镁盐矿床盐井卤水中分离出的新菌株 MX2。该菌株是一种需氧、革兰氏阳性、不运动的细菌，不形成孢子。细胞形态从球菌到短杆菌不等，能形成 V 型。琼脂上的菌落呈圆形，边缘完整，中心凸起，晶莹剔透，呈橙色。菌株 MX2 是一种耐盐微生物，能在 NaCl 浓度高达 9% 的条件下生长。对菌株 MX2 的基因组进行了测序。据估计，其基因组大小为 3 747 717 bp，编码蛋白质的基因数量为 3562 个。根据 16S rRNA、gyrB、rpoB、recA 和 ppk 基因序列分析和飞行时间质谱（MALDI-TOF-MS）分析，确定菌株 MX2 属于 Dietzia maris。D. maris MX2 具有合成埃克托因、羟基埃克托因和三卤糖的完整代谢途径，以及埃克托因、羟基埃克托因、三卤糖、甘油、甘油-3-磷酸、L-脯氨酸和甘氨酸-甜菜碱的转运系统。因此，为了确保渗透平衡，D. maris MX2 采用了积累兼容有机溶质的策略。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.