Microbiology最新文献_第2页

Effect of Environmental Factors on Recombinant Activity of Root Nodule Bacteria 环境因素对根瘤菌重组活性的影响

IF 1.5 4区生物学

Microbiology Pub Date : 2024-07-26 DOI: 10.1134/s0026261724604858

An. Kh. Baymiev, I. S. Koryakov, E. S. Akimova, A. A. Vladimirova, Al. Kh. Baymiev

{"title":"Effect of Environmental Factors on Recombinant Activity of Root Nodule Bacteria","authors":"An. Kh. Baymiev, I. S. Koryakov, E. S. Akimova, A. A. Vladimirova, Al. Kh. Baymiev","doi":"10.1134/s0026261724604858","DOIUrl":"https://doi.org/10.1134/s0026261724604858","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000Abstract—</h3>The legume–rhizobia symbiosis is a unique natural phenomenon, which supplies the plant with the necessary mineral nitrogen via fixation of atmospheric dinitrogen. This interaction involves two partners: the legume plant and root nodule bacteria (rhizobia). In the wild, members of the Fabaceae family enter into symbiosis with a polymorphic group of rhizobia specific to them; the mechanism and reasons for the formation of heterogeneity of rhizobia are currently the subject of active research. In the present work, a Rhizobium leguminosarum strain strictly specific to Phaseolus vulgaris L. was used to show that within 30 days upon its introduction into soil, genetic rearrangements occurred in the cells, as was revealed by changes in the pattern of its genetic profile. It was also found that recombination activity of the cells was also affected by the root exudates produced during seed germination, which may indicate involvement of the plant in the formation of polymorphism of its microsymbionts. These findings suggest interpretation of this process not as a spontaneous event, but rather as an event controlled by the plant.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141770041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Activities and Characterization of Bacteriocin-Like Substances Isolated from Lactic Acid Bacteria of Local Fermented Foods 从本地发酵食品的乳酸菌中分离出的类细菌素物质的抗菌活性和特性分析

IF 1.5 4区生物学

Microbiology Pub Date : 2024-07-26 DOI: 10.1134/s0026261723601999

R. C. Inovejas, C. C. Divina, J. K. Jacob, J. B. Abucay Jr., E. L. C. Inovejas

{"title":"Antimicrobial Activities and Characterization of Bacteriocin-Like Substances Isolated from Lactic Acid Bacteria of Local Fermented Foods","authors":"R. C. Inovejas, C. C. Divina, J. K. Jacob, J. B. Abucay Jr., E. L. C. Inovejas","doi":"10.1134/s0026261723601999","DOIUrl":"https://doi.org/10.1134/s0026261723601999","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Bacteriocins produced by a number of lactic acid bacteria (LAB) comprise a variety of antimicrobial proteins that may be used as antibiotic alternative in food preservation. The present study aimed to isolate and characterize LAB-derived bacteriocin-like substances (BLS) from local fermented foods. A total of twenty-one bacterial strains were isolated and subjected to an antibacterial assay. Five isolates designated as AB1, AB2, AK1, AK2, AnK1 showed inhibitory activity against selected indicator organisms and were subjected to further characterization. Upon BLAST analysis of the 16S rRNA gene sequences, the isolates showed close relationship to Lactobacillus plantarum with similarity ranging from 91 to 97%. Moreover, the antibacterial metabolite production of the isolates was observed to peak on the 5th to 7th day of incubation. Since AnK1 showed the highest zone of inhibition among 5 isolates, it was selected for characterization of the BLS isolated by ammonium sulfate precipitation and anion exchange chromatography. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the AnK1-BLS had a single band and a molecular weight around 15 kDa, and a total protein concentration of 7.06 µg/mL. Interestingly, AnK1-BLS had a broad antimicrobial spectrum on both gram-negative and gram-positive food-borne pathogens, including Escherichia coli, Salmonella enterica, Pseudomonas aeruginosa, and Bacillus subtilis. Moreover, the purified AnK1-BLS exhibited good thermal stability when heated at 80‒121°C. Likewise, its inhibitory activity was not affected by pH in the range of 3.0 to 6.5, with maximum activity recorded at pH 3.0. The results suggest that AnK1-BLS may have application prospects in the food industry. Further studies on the complete protein characterization of AnK1-BLS is recommended to fully harness its capabilities.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141770043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgae from Eroded Soils in the Northern Fergana Valley, Uzbekistan 乌兹别克斯坦费尔干纳河谷北部侵蚀土壤中的微藻类

IF 1.5 4区生物学

Microbiology Pub Date : 2024-07-26 DOI: 10.1134/s0026261724605013

Yu. A. Tukhtaboeva, E. S. Krivina, V. V. Red’kina, A. D. Temraleeva

{"title":"Microalgae from Eroded Soils in the Northern Fergana Valley, Uzbekistan","authors":"Yu. A. Tukhtaboeva, E. S. Krivina, V. V. Red’kina, A. D. Temraleeva","doi":"10.1134/s0026261724605013","DOIUrl":"https://doi.org/10.1134/s0026261724605013","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000Abstract\u0000</h3>For the first time, the cultivated diversity of microalgae in eroded soils in the northern part of the Fergana Valley in Uzbekistan has been studied based on both morphological and molecular genetic analysis. Ten strains of green microalgae (Chlorophyta) and one charophyte strain (Charophyta) were revealed. Only seven strains could be identified at the species level: Chlorella vulgaris, Chromochloris zofingiensis, Deuterostichococcus epilithicus, Pseudomuriella schumacherensis, and Pseudostichococcus monallantoides. Another four strains were identified only at the genus level and require further study: Bracteacoccus sp., Chlorosarcinopsis sp., Klebsormidium sp., and Tetratostichococcus sp. The low species diversity in the microalgae is likely due to both the low fertility of the eroded soils on the slopes, and the limitations of the culture-based approach that only reveals a fraction of the overall microbial diversity. Microalgal colonization of eroded soils in the arid foothill zone can be facilitated by various adaptations, such as small cell size and the production of extracellular polysaccharides, mycosporine-like aminoacids, and secondary carotenoids. The present work may contribute to the further development of highly functional microalgal consortia, which can lead to improvements and sustainable development of low-productivity, arid, and degraded terrestrial ecosystems.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141770039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Bioactive Potential and Characterization of an Anticancer Compound from the Endophytic Fungi of Ocimum sanctum 评估欧琴圣草内生真菌中一种抗癌化合物的生物活性潜力并确定其特性

IF 1.5 4区生物学

Microbiology Pub Date : 2024-07-26 DOI: 10.1134/s002626172360252x

A. Verma, K. Kumar, U. Talukdar, G. Pal, D. Kumar, P. Shukla, S. Patel, A. Kumar, A. Kumar, R. N. Kharwar, S. K. Verma

{"title":"Assessment of Bioactive Potential and Characterization of an Anticancer Compound from the Endophytic Fungi of Ocimum sanctum","authors":"A. Verma, K. Kumar, U. Talukdar, G. Pal, D. Kumar, P. Shukla, S. Patel, A. Kumar, A. Kumar, R. N. Kharwar, S. K. Verma","doi":"10.1134/s002626172360252x","DOIUrl":"https://doi.org/10.1134/s002626172360252x","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The goal of this study was to isolate endophytic fungi from Ocimum sanctum L. and to evaluate their biological potential, including antibacterial, antioxidant, and enzymatic activities and further characterization of the bioactive compounds. Nine endophytic fungi were isolated from the leaves and stem tissues of O. sanctum collected from the botanical garden of Banaras Hindu University, Varanasi, India. All isolates were identified based on their microscopic structures and molecular sequencing of the ITS rDNA. Aspergillus clavatonanicus (SS7) and Cochliobolus hawaiiensis (SL3) showed the highest colonization frequencies in the stem and leaves (16 and 14%, respectively). All fungal isolates were tested for extracellular enzymatic activities of amylase, cellulase, and pectinase. Of the nine fungal isolates, 60% tested positive for amylase and cellulase, whereas 50% showed pectinase activity. Using a disc diffusion assay, the extracted secondary metabolites were checked for antibacterial activity against three human pathogenic bacteria. Two isolates, SL2 and SS7, exhibited the highest antibacterial activity against all pathogens, including Enterococcus faecalis, Klebsiella pneumoniae, and methicillin-resistant Staphylococcus aureus (MRSA). Crude extracts of the six fungal isolates showed positive antioxidant activity. The crude extract of Aspergillus allahabadii (isolate SL2) showed strong antibacterial and antioxidant activities and crystallized during purification. X-ray crystallography confirmed the identity of the crystal as citrinin, which also exhibited strong anticancer activity against Dalton’s lymphoma cells. The results of this study suggest that endophytic fungi isolated from the leaf and stem tissues of Ocimum sanctum are potential sources of antibacterial, antioxidant, and anticancer compounds.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141770042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Electrical Stimulation of the External Circuit of Membraneless Bioelectrochemical Systems on Imidacloprid Degradation and Representation of the mtrB and DyP-Type Peroxidases Genes 无膜生物电化学系统外电路的电刺激对吡虫啉降解的影响以及 mtrB 和 DyP 型过氧化物酶基因的表征

IF 1.5 4区生物学

Microbiology Pub Date : 2024-07-26 DOI: 10.1134/s0026261723604396

A. A. Samkov, S. M. Samkova, M. N. Kruglova

{"title":"Effect of Electrical Stimulation of the External Circuit of Membraneless Bioelectrochemical Systems on Imidacloprid Degradation and Representation of the mtrB and DyP-Type Peroxidases Genes","authors":"A. A. Samkov, S. M. Samkova, M. N. Kruglova","doi":"10.1134/s0026261723604396","DOIUrl":"https://doi.org/10.1134/s0026261723604396","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\u0000Abstract\u0000</h3>In all variants where carbon felt was present, imidacloprid degradation by the microflora of bottom sediments was many times higher than in the control, reaching 84.0 ± 1.7% under the polar connection of an external voltage source (1.2 V). When Shewanella oneidensis MR-1 was introduced, almost complete degradation of the pollutant was observed, while in the control without electrodes, it was 29.7 ± 6.0%. The relative representation of the genes of the MtrB transmembrane protein of the respiratory chain, which is associated with exoelectrogenesis, depended on the external chain and had a maximum value when the voltage source was connected polarly, correlating with the pesticide degradation by the autochthonous microflora, similar to the DyP-type peroxidase genes. The introduction of S. oneidensis MR-1 resulted in an almost tenfold increase in the relative representation of DyP-type peroxidase genes. In all experimental variants, the values of the DyP relative representation were significantly higher than in the control without carbon felt, as well as the degree of imidacloprid degradation under these experimental conditions.","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141770047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Search for Bacteriophages Specific against Members of the Genus Rhodococcus 寻找特异性噬菌体来对付 Rhodococcus 属的成员

IF 1.5 4区生物学

Microbiology Pub Date : 2024-07-26 DOI: 10.1134/s0026261723604979

A. D. Novikov, I. P. Tokmakova, A. A. Samarin, K. V. Lavrov, A. S. Yanenko

引用次数: 0

The Effect of Probiotic Lactobacilli on the Morphological and Physiological Parameters and Intestinal Microbiota of Quails 益生菌乳酸杆菌对鹌鹑形态、生理参数和肠道微生物群的影响

IF 1.5 4区生物学

Microbiology Pub Date : 2024-07-26 DOI: 10.1134/s0026261723604669

E. A. Gavrilova, O. S. Karaseva, Y. N. Monir, A. M. Ezhkova, V. O. Ezhkov, R. A. Volkov, A. M. Senina, D. R. Khusnutdinova, E. V. Nikitina, D. R. Yarullina, A. R. Kayumov

引用次数: 0

Petrocella pelovolcani sp. nov., an Alkaliphilic Anaerobic Bacterium Isolated from Terrestrial Mud Volcano 从陆地火山泥中分离出的嗜碱性厌氧细菌 Petrocella pelovolcani sp.

IF 1.5 4区生物学

Microbiology Pub Date : 2024-07-26 DOI: 10.1134/s0026261724605803

A. A. Frolova, A. Yu. Merkel, D. S. Kopitsyn, A. I. Slobodkin

{"title":"Petrocella pelovolcani sp. nov., an Alkaliphilic Anaerobic Bacterium Isolated from Terrestrial Mud Volcano","authors":"A. A. Frolova, A. Yu. Merkel, D. S. Kopitsyn, A. I. Slobodkin","doi":"10.1134/s0026261724605803","DOIUrl":"https://doi.org/10.1134/s0026261724605803","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Diversity of extremophilic microorganisms in mud volcanoes is largely unexplored. Here we report the isolation of a novel alkaliphilic, mesophilic, fermentative bacterium (strain FN5sucT) from a terrestrial mud volcano located at the Taman Peninsula, Russia. Cells of strain FN5sucT are gram-stain-positive, non-sporeforming, motile rods. The temperature range for growth is 10–37°C, with an optimum at 30°C. The pH range for growth is 7.5–10.0, with an optimum at pH 9.0. The isolate utilizes various organic polymeric substances, organic acids, carbohydrates, and proteinaceous compounds. The end products of carbohydrates fermentation are acetate, CO2 and trace amounts of H2 and formate. The major cellular fatty acid compounds are C16:0, C16:1 ω7c, and monounsaturated dimethyl acetal C14:1. Phylogenetic analysis reveals that strain FN5sucT is most closely related to Petrocella atlantisensis = DSM 105309T (98.4% 16S rRNA gene identity). The total size of the genome of strain FN5sucT is 3.35 Mb, and a genomic DNA G+C content is 37.0 mol %. The genome contains complete glycolisis/glyconeogenesis pathway. We propose to assign strain FN5sucT to the genus Petrocella, as a new species, Petrocella pelovolcani sp. nov. The type strain is FN5sucT (=DSM 113898T = UQM 41591T).","PeriodicalId":18514,"journal":{"name":"Microbiology","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141770133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pymetrozine Degradation by an Enrichment Culture from Paddy Soil 稻田土壤富集培养物对百菌清的降解作用

IF 1.5 4区生物学

Microbiology Pub Date : 2024-05-14 DOI: 10.1134/s002626172360249x

H. D. Duc, N. T. Oanh

引用次数: 0

Antimicrobial Activity of Terpens and Oxygen-Containing Terpenoids against Staphylococcus aureus 萜类和含氧萜类化合物对金黄色葡萄球菌的抗菌活性

IF 1.5 4区生物学

Microbiology Pub Date : 2024-05-14 DOI: 10.1134/S002626172360427X

A. Kolesnikova, A. Kayumov, I. R. Gilfanov, L. L. Frolova, L. E. Nikitina, E. Trizna

引用次数: 0