Specific Response of Bacterial Cells to the Action of β-Ionone

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-02 DOI:10.1134/s0026261723604268

D. E. Sidorova, O. E. Melkina, O. A. Koksharova, E. N. Vagner, I. A. Khmel, V. A. Plyuta

引用次数: 0

Abstract

The biological activity and mechanisms of action of the unsaturated ketone β-ionone, a volatile compound of serious interest for biotechnology, medicine, and agriculture, have been studied. Using specific lux-biosensors based on Escherichia coli MG1655, we found that β-ionone causes oxidative stress in E. coli cells by inducing expression from the PkatG and Pdps promoters, but not from PsoxS promoter. The effects of β-ionone on the induction of heat shock (expression from PibpA and PgrpE promoters) in E. coli cells were significantly weaker as well as its ability to cause DNA damage (expression from PcolD and PdinI promoters, SOS response). β-Ionone did not cause oxidative stress in the cells of the gram-positive bacterium Bacillus subtilis.

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细菌细胞对 β-酮作用的特殊反应

摘要我们研究了不饱和酮β-酮的生物活性和作用机制，β-酮是一种挥发性化合物，对生物技术、医药和农业具有重大意义。我们利用基于大肠杆菌 MG1655 的特异性勒克斯生物传感器发现，β-酮通过诱导 PkatG 和 Pdps 启动子（而非 PsoxS 启动子）的表达，导致大肠杆菌细胞产生氧化应激。β-ionone 对大肠杆菌细胞热休克（PibpA 和 PgrpE 启动子的表达）的诱导作用明显较弱，对 DNA 损伤（PcolD 和 PdinI 启动子的表达、SOS 反应）的诱导作用也明显较弱。在革兰氏阳性细菌枯草芽孢杆菌的细胞中，β-壬酮不会引起氧化应激。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.