Petrocella pelovolcani sp. nov., an Alkaliphilic Anaerobic Bacterium Isolated from Terrestrial Mud Volcano

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-26 DOI:10.1134/s0026261724605803

A. A. Frolova, A. Yu. Merkel, D. S. Kopitsyn, A. I. Slobodkin

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Abstract

Diversity of extremophilic microorganisms in mud volcanoes is largely unexplored. Here we report the isolation of a novel alkaliphilic, mesophilic, fermentative bacterium (strain FN5suc^T) from a terrestrial mud volcano located at the Taman Peninsula, Russia. Cells of strain FN5suc^T are gram-stain-positive, non-sporeforming, motile rods. The temperature range for growth is 10–37°C, with an optimum at 30°C. The pH range for growth is 7.5–10.0, with an optimum at pH 9.0. The isolate utilizes various organic polymeric substances, organic acids, carbohydrates, and proteinaceous compounds. The end products of carbohydrates fermentation are acetate, CO₂ and trace amounts of H₂ and formate. The major cellular fatty acid compounds are C_16:0, C_{16:1 ω7c}, and monounsaturated dimethyl acetal C_14:1. Phylogenetic analysis reveals that strain FN5suc^T is most closely related to Petrocella atlantisensis = DSM 105309^T (98.4% 16S rRNA gene identity). The total size of the genome of strain FN5suc^T is 3.35 Mb, and a genomic DNA G+C content is 37.0 mol %. The genome contains complete glycolisis/glyconeogenesis pathway. We propose to assign strain FN5suc^T to the genus Petrocella, as a new species, Petrocella pelovolcani sp. nov. The type strain is FN5suc^T (=DSM 113898^T = UQM 41591^T).

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从陆地火山泥中分离出的嗜碱性厌氧细菌 Petrocella pelovolcani sp.

摘要泥火山中嗜极端微生物的多样性在很大程度上尚未得到探索。本文报告了从俄罗斯塔曼半岛的一座陆地泥火山中分离出的一种新型嗜碱、嗜中性发酵细菌（菌株 FN5sucT）。菌株 FN5sucT 的细胞为革兰氏染色阳性、无芽孢、运动棒状。生长温度范围为 10-37°C，最适温度为 30°C。生长的 pH 值范围为 7.5-10.0，最适 pH 值为 9.0。该分离菌利用各种有机聚合物质、有机酸、碳水化合物和蛋白质化合物。碳水化合物发酵的最终产物是醋酸盐、二氧化碳以及微量的 H2 和甲酸盐。细胞中的主要脂肪酸化合物是 C16:0、C16:1 ω7c 和单不饱和二甲基缩醛 C14:1。系统进化分析表明，菌株 FN5sucT 与 Petrocella atlantisensis = DSM 105309T 的亲缘关系最密切（16S rRNA 基因相同度为 98.4%）。菌株 FN5sucT 的基因组总大小为 3.35 Mb，基因组 DNA G+C 含量为 37.0 mol%。基因组包含完整的糖化/糖酮生成途径。我们建议将 FN5sucT 菌株归入石化菌属，作为一个新种--Petrocella pelovolcani sp.模式菌株为 FN5sucT（=DSM 113898T = UQM 41591T）。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.