Metabolites最新文献

{"title":"Metabolic Reprogramming of Gastric Cancer Revealed by a Liquid Chromatography-Mass Spectrometry-Based Metabolomics Study.","authors":"Lina Zhou, Benzhe Su, Zexing Shan, Zhenbo Gao, Xingyu Guo, Weiwei Wang, Xiaolin Wang, Wenli Sun, Shuai Yuan, Shulan Sun, Jianjun Zhang, Guowang Xu, Xiaohui Lin","doi":"10.3390/metabo15040222","DOIUrl":"https://doi.org/10.3390/metabo15040222","url":null,"abstract":"<p><strong>Background/objectives: </strong>Gastric cancer (GC) is a prevalent malignant tumor worldwide, with its pathological mechanisms largely unknown. Understanding the metabolic reprogramming associated with GC is crucial for the prevention and treatment of this disease. This study aims to identify significant alterations in metabolites and pathways related to the development of GC.</p><p><strong>Methods: </strong>A liquid chromatography-mass spectrometry-based non-targeted metabolomics data acquisition was performed on paired tissues from 80 GC patients. Differences in metabolic profiles between tumor and adjacent normal tissues were first investigated through univariate and multivariate statistical analyses. Additionally, differential correlation network analysis and a newly proposed network analysis method (NAM) were employed to explore significant metabolite pathways and subnetworks related to tumorigenesis and various TNM stages of GC.</p><p><strong>Results: </strong>Over half of the annotated metabolites exhibited significant alterations. Phosphatidylcholine (PC)_30_0 and fatty acid C20_3 demonstrated strong diagnostic performance for GC, with AUCs of 0.911 and 0.934 in the discovery and validation sets, respectively. Differential correlation network analysis revealed significant fatty acid-related metabolic reprogramming in GC with elevated levels of medium-chain acylcarnitines and increased activity of medium-chain acyl-CoA dehydrogenase, firstly observed in clinical GC tissues. Of note, using NAM, two correlation subnetworks were identified as having significant alterations across different TNM stages, centered with choline and carnitine C4_0-OH, respectively.</p><p><strong>Conclusions: </strong>The identified significant alterations in fatty acid metabolism and TNM-related metabolic subnetworks in GC tissues will facilitate future investigations into the metabolic reprogramming associated with gastric cancer.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current Prognostic Biomarkers for Peripheral Arterial Disease: A Comprehensive Systematic Review of the Literature.","authors":"Hamzah Khan, Natasha R Girdharry, Sophia Z Massin, Mohamed Abu-Raisi, Gustavo Saposnik, Muhammad Mamdani, Mohammad Qadura","doi":"10.3390/metabo15040224","DOIUrl":"https://doi.org/10.3390/metabo15040224","url":null,"abstract":"<p><p><b>Background:</b> Peripheral arterial disease (PAD) is a chronic atherosclerotic disease characterized by atheromatous plaque buildup within arteries of the lower limbs. It can lead to claudication, skin ulcerations, and, in severe cases, chronic limb-threatening ischemia, requiring amputation. There are several plasma protein biomarkers that have been suggested as prognostic markers for adverse events, including major adverse cardiovascular and limb events. However, the clinical benefit and ability to clinically adapt these biomarkers remains uncertain due to inconsistent findings possibly related to heterogenous study designs and differences in methodology. <b>Objectives:</b> This review aims to evaluate the current literature on the prognostic value of plasma protein biomarkers for PAD, their predictive ability for PAD-related adverse outcomes, and their potential roles in guiding PAD management. <b>Methods:</b> To address these challenges, we conducted a systematic review of MEDLINE, Embase, and Cochrane CENTRAL libraries of the current literature (2010-2024). <b>Results:</b> We found 55 studies that evaluated the prognostic value of 44 distinct plasma proteins across various pathophysiological processes. These included markers of immunity and inflammation, markers of metabolism, cardiac biomarkers, markers of kidney function, growth factors and hormones, markers of coagulation and platelet function, extracellular matrix and tissue remodeling proteins, and transport proteins. This review summarizes the existing evidence for prognostic protein plasma biomarkers for PAD and their association with adverse events related to PAD. Conclusions: With this review, we hope to provide a comprehensive list of the prognostic markers and their value as prognostic biomarkers to guide clinical decision making in these patients.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Niches and Plasticity of Sand-Dune Plant Communities Along a Trans-European Gradient.","authors":"Matthew P Davey, Rachel M George, Mark K J Ooi, Mike M Burrell, Robert P Freckleton","doi":"10.3390/metabo15040217","DOIUrl":"https://doi.org/10.3390/metabo15040217","url":null,"abstract":"<p><p><b>Background:</b> One of the greatest challenges to biologists is to understand the adaptive mechanisms of how plants will respond to climate at all levels from individual physiology to whole populations. For example, variation (plasticity) in the composition and concentration of metabolites will determine productivity, reproduction, and ultimately survival and distribution of plants, especially those subjected to rapid climate change. <b>Objectives:</b> Our aim was to study how interspecific and intraspecific metabolic variation in plant species within a single community can be elucidated. <b>Methods:</b> We used a metabolomics approach to study metabolic acclimation (by measuring the metabolome between plants under \"common garden\" controlled environment conditions) and metabolic plasticity (using field based reciprocal transplant studies) in a set of Atlantic sand dune annual communities along a latitudinal gradient from Portugal to England. <b>Results:</b> In the common garden study, metabolically phenotyping (using a fingerprinting direct injection mass spectrometry approach) five species of annual plants showed that species living together in a community have distinct metabolic phenotypes (high inter-specific metabolic variation). There was low intra-specific metabolic variation between populations growing under standard environmental conditions. The metabolic variation in one species <i>Veronica arvensis</i> was measured in the reciprocal transplant study. Metabolic phenotypes obtained from all samples were similar across all sites regardless of where the plants originated from. <b>Conclusions:</b> This implies that the metabolome is highly plastic and the measurable metabolome in this study was influenced more by local environmental factors than inherent genetic factors. This work highlights that species are fulfilling different niches within this community. Furthermore, the measurable metabolome was highly plastic to environmental variation.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trimethylamine-N-Oxide (TMAO) as a Rising-Star Metabolite: Implications for Human Health.","authors":"Eugenio Caradonna, Federico Abate, Elisabetta Schiano, Francesca Paparella, Fulvio Ferrara, Emilio Vanoli, Rossana Difruscolo, Vito Maria Goffredo, Bruno Amato, Carlo Setacci, Francesco Setacci, Ettore Novellino","doi":"10.3390/metabo15040220","DOIUrl":"https://doi.org/10.3390/metabo15040220","url":null,"abstract":"<p><p>The intestinal microbiota, hosting trillions of microorganisms that inhabit the gastrointestinal tract, functions as a symbiotic organism that plays a crucial role in regulating health by producing biologically active molecules that can enter systemic circulation. Among them, trimethylamine-N-oxide (TMAO), an organic compound derived from dietary sources and microbial metabolism, has emerged as a critical biomarker linking diet, the gut microbiota, and the host metabolism to various pathological conditions. This comprehensive review highlights TMAO's biosynthesis, physiological functions, and clinical significance, focusing on its mechanistic contributions to cardiovascular and neurodegenerative diseases. Notably, TMAO-mediated pathways include endothelial dysfunction, inflammation via NLRP3 inflammasome activation, and cholesterol metabolism disruption, which collectively accelerate atherosclerosis and disease progression. Nonetheless, this work underscores the innovative potential of targeting TMAO through dietary, nutraceutical, and microbiota-modulating strategies to mitigate its pathological effects, marking a transformative approach in the prevention and management of TMAO-related disorders.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoplasmic Reticulum Stress and Its Role in Metabolic Reprogramming of Cancer.","authors":"Salvatore Zarrella, Maria Rosaria Miranda, Verdiana Covelli, Ignazio Restivo, Sara Novi, Giacomo Pepe, Luisa Tesoriere, Manuela Rodriquez, Alessia Bertamino, Pietro Campiglia, Mario Felice Tecce, Vincenzo Vestuto","doi":"10.3390/metabo15040221","DOIUrl":"https://doi.org/10.3390/metabo15040221","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Endoplasmic reticulum (ER) stress occurs when ER homeostasis is disrupted, leading to the accumulation of misfolded or unfolded proteins. This condition activates the unfolded protein response (UPR), which aims to restore balance or trigger cell death if homeostasis cannot be achieved. In cancer, ER stress plays a key role due to the heightened metabolic demands of tumor cells. This review explores how metabolomics can provide insights into ER stress-related metabolic alterations and their implications for cancer therapy. <b>Methods:</b> A comprehensive literature review was conducted to analyze recent findings on ER stress, metabolomics, and cancer metabolism. Studies examining metabolic profiling of cancer cells under ER stress conditions were selected, with a focus on identifying potential biomarkers and therapeutic targets. <b>Results:</b> Metabolomic studies highlight significant shifts in lipid metabolism, protein synthesis, and oxidative stress management in response to ER stress. These metabolic alterations are crucial for tumor adaptation and survival. Additionally, targeting ER stress-related metabolic pathways has shown potential in preclinical models, suggesting new therapeutic strategies. <b>Conclusions:</b> Understanding the metabolic impact of ER stress in cancer provides valuable opportunities for drug development. Metabolomics-based approaches may help identify novel biomarkers and therapeutic targets, enhancing the effectiveness of antitumor therapies.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-Term Supplementation with 100% Bilberry Products and Its Effects on Body Composition and Lipid Profile in Overweight/Obese Women.","authors":"Marta Habanova, Maros Bihari, Radek Latal, Martina Gažarova, Petra Lenártová, Jana Pastrnakova, Jadwiga Hamulka","doi":"10.3390/metabo15040218","DOIUrl":"https://doi.org/10.3390/metabo15040218","url":null,"abstract":"<p><p><b>Introduction</b>: Overweight and obesity are major public health concerns, often leading to increased cardiovascular risk. <b>Methods</b>: This eight-week interventional study examined whether regular consumption of two natural bilberry products could improve body composition and lipid profiles in overweight/obese women. A total of 30 participants (aged 50-60 years) were assigned to consume either 125 mL/day of 100% bilberry juice or 10 g/day of 100% bilberry fibre, while maintaining their habitual diets and lifestyles. <b>Results</b>: Although no significant changes were found in anthropometric parameters or blood pressure in either group, both interventions reduced low-density lipoprotein cholesterol (LDL-C) and increased high-density lipoprotein cholesterol (HDL-C). Surprisingly, total cholesterol (TC) levels rose in the bilberry juice group (from 6.41 ± 1.23 mmol/L to 6.94 ± 1.30 mmol/L (<i>p</i> < 0.001)), and in the fibre group (from 6.06 ± 1.39 mmol/L to 6.43 ± 1.05 mmol/L (<i>p</i> = 0.046)), likely due to elevated HDL-C (<i>p</i> < 0.001) overshadowing the drop in LDL-C (<i>p</i> < 0.05). Triglyceride (TG) levels did not change significantly and were still within the reference range. <b>Conclusions</b>: Notably, the bilberry juice group experienced a significant reduction in atherogenic small dense LDL (sdLDL) subfractions, suggesting a favourable shift in cardiovascular risk factors. These findings highlight the potential of bilberry-based products as a supportive strategy for improving lipid profiles in overweight/obese women.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycotoxins in Feed: Hazards, Toxicology, and Plant Extract-Based Remedies.","authors":"Xiangnan Zhang, Jiashun Chen, Xiaokang Ma, Xiongzhuo Tang, Bie Tan, Peng Liao, Kang Yao, Qian Jiang","doi":"10.3390/metabo15040219","DOIUrl":"https://doi.org/10.3390/metabo15040219","url":null,"abstract":"<p><p><b>Background:</b> Mycotoxins, which are secondary metabolites produced by fungi, are prevalent in animal feed and pose a serious risk to the healthy growth of livestock and poultry. <b>Methods:</b> This review aims to conclude current knowledge on the detrimental effects of mycotoxins on animal health and to demonstrate the potential of plant extracts as a means to counteract mycotoxin toxicity in feed. A systematic review of the literature was conducted to identify studies on the impact of mycotoxins on livestock and poultry health, as well as research into the use of plant extracts as feed additives to mitigate mycotoxin effects. Studies were selected based on their relevance to the topic, and data were extracted regarding the mechanisms of action and the efficacy of plant extracts. <b>Results:</b> Excessive mycotoxins in feed can lead to reduced appetite, impaired digestion, and general health issues in animals, resulting in decreased food intake, slowed weight gain, and instances of acute poisoning. Plant extracts with antioxidant, anti-inflammatory, and anti-mutagenic properties have shown the potential to improve production efficiency and reduce the toxic effects of mycotoxins. <b>Conclusion:</b> This comprehensive review not only consolidates the well-documented adverse effects of mycotoxins on animal health but also introduces a novel perspective by highlighting the potential of plant extracts as a promising and natural solution to counteract mycotoxin toxicity.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secondary Metabolites from <i>Croton</i> Species and Their Biological Activity on Cell Cycle Regulators.","authors":"Jorge Augusto Alamillo-Vásquez, Claudia-Anahí Pérez-Torres, Enrique Ibarra-Laclette, Feliza Ramón-Farías, Pilar Nicasio-Torres, Fulgencio Alatorre-Cobos","doi":"10.3390/metabo15040216","DOIUrl":"https://doi.org/10.3390/metabo15040216","url":null,"abstract":"<p><p>Plant-based traditional medicine integrates beliefs, knowledge, and practices to prevent and treat multiple diseases. <i>Croton</i> is a large and worldwide-spread genus belonging to Euphorbiaceae, a family well known for comprising many species with medicinal properties due to its high diversity of phytochemical constituents with biological activities. Among the various benefits of <i>Croton</i> species in traditional medicine, its use in cancer treatment has recently received significant attention from the scientific community. This review provides a general overview of different studies on the <i>Croton</i> genus in the research for alternative cancer treatments and the impact of its secondary metabolite catalog on cell cycle targets. Our analysis indicates that just under 30 secondary metabolites have been identified so far in latex and extracts obtained from leaves, twigs, or bark from 22 different <i>Croton</i> species. Based on standard assays using cell lines or human platelets, these molecules show multiple biological activities mainly compromising cell viability and cell cycle progression, supporting the ethnobotanical use of <i>Croton</i> species for cancer treatment. Several studies indicate that <i>Croton</i> metabolites target CDK-cyclin complexes and signaling routes that trigger apoptosis; however, further studies are needed to better understand the molecular mechanisms underlying <i>Croton</i> metabolites' effects and their accurate future applications in cancer treatment.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the Dietary Fat Concentration and Source on the Fecal Microbiota of Healthy Adult Cats.","authors":"Nadine Paßlack, Kathrin Büttner, Wilfried Vahjen, Jürgen Zentek","doi":"10.3390/metabo15040215","DOIUrl":"https://doi.org/10.3390/metabo15040215","url":null,"abstract":"<p><strong>Background/objectives: </strong>The dietary fat supply might interact with the intestinal microbiota via different mechanisms. Research on this topic, however, remains scarce in cats. For this reason, the present study was conducted to evaluate the impact of the fat concentration and fatty acid profile in the diet on the fecal microbiota of healthy cats.</p><p><strong>Methods: </strong>A low-fat basal diet was fed to ten healthy adult cats. The diet was offered without or with the daily addition of 0.5 g or 1 g of sunflower oil, fish oil or lard per kg body weight of the cats, using a randomized cross-over design. Each feeding period lasted for 21 days, and the fecal samples were collected on the last days of each period. The fecal microbiota was analyzed by 16S rDNA sequencing. Additionally, microbial metabolites (short-chain fatty acids, lactate, ammonium, biogenic amines) were measured in the fecal samples.</p><p><strong>Results: </strong>The dietary treatment had no impact on the alpha-diversity of the fecal microbiota or on the relative abundance of bacterial phyla in the samples. Only a few changes were observed in the relative abundance of bacterial genera and the concentrations of microbial metabolites in the feces, probably being of minor physiological relevance.</p><p><strong>Conclusions: </strong>The balanced intestinal microbiota of cats seems to be relatively resistant to moderate variations in the dietary fat supply over a short feeding period. Longer-term treatments and higher dietary fat levels should be evaluated in future studies to further clarify the relevance of fat intake for the feline gut microbiome.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12028789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double-Weighted Bayesian Model Combination for Metabolomics Data Description and Prediction.","authors":"Jacopo Troisi, Martina Lombardi, Alessio Trotta, Vera Abenante, Andrea Ingenito, Nicole Palmieri, Sean M Richards, Steven J K Symes, Pierpaolo Cavallo","doi":"10.3390/metabo15040214","DOIUrl":"https://doi.org/10.3390/metabo15040214","url":null,"abstract":"<p><strong>Background/objectives: </strong>This study presents a novel double-weighted Bayesian Ensemble Machine Learning (DW-EML) model aimed at improving the classification and prediction of metabolomics data. This discipline, which involves the comprehensive analysis of metabolites in a biological system, provides valuable insights into complex biological processes and disease states. As metabolomics assumes an increasingly prominent role in the diagnosis of human diseases and in precision medicine, there is a pressing need for more robust artificial intelligence tools that can offer enhanced reliability and accuracy in medical applications. The proposed DW-EML model addresses this by integrating multiple classifiers within a double-weighted voting scheme, which assigns weights based on the cross-validation accuracy and classification confidence, ensuring a more reliable prediction framework.</p><p><strong>Methods: </strong>The model was applied to publicly available datasets derived from studies on critical illness in children, chronic typhoid carriage, and early detection of ovarian cancer.</p><p><strong>Results: </strong>The results demonstrate that the DW-EML approach outperformed methods traditionally used in metabolomics, such as the Partial Least Squares Discriminant Analysis in terms of accuracy and predictive power.</p><p><strong>Conclusions: </strong>The DW-EML model is a promising tool for metabolomic data analysis, offering enhanced robustness and reliability for diagnostic and prognostic applications and potentially contributing to the advancement of personalized and precision medicine.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}