Metabolites最新文献

{"title":"Integration and Analysis of Omics Data Using Genome-Scale Metabolic Models.","authors":"Miha Moškon, Tadeja Režen","doi":"10.3390/metabo14110595","DOIUrl":"10.3390/metabo14110595","url":null,"abstract":"<p><p>Constraint-based modelling and genome-scale metabolic models (GEMs) have been used extensively to analyze omics data, providing a mechanistic perspective on complex metabolic systems and networks [...].</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biostimulant-Based Molecular Priming Improves Crop Quality and Enhances Yield of Raspberry and Strawberry Fruits.","authors":"Petar Kazakov, Saleh Alseekh, Valentina Ivanova, Tsanko Gechev","doi":"10.3390/metabo14110594","DOIUrl":"10.3390/metabo14110594","url":null,"abstract":"<p><strong>Background/objectives: </strong>The biostimulant SuperFifty, produced from the brown algae <i>Ascophyllum nodosum</i>, can improve crop quality and yield and mitigate stress tolerance in model and crop plants such as <i>Arabidopsis thaliana</i>, pepper, and tomato. However, the effect of SuperFifty on raspberries and strawberries has not been well studied, especially in terms of nutritional properties and yield. The aim of this study was to investigate the effect of SuperFifty on the quality and quantity of raspberry and strawberry fruits, with a focus on metabolic composition and essential elements, which together determine the nutritional properties and total yield of these two crops.</p><p><strong>Methods: </strong>Metabolome analysis was performed by liquid chromatography-mass spectrometry analysis (LC-MS), and essential elements analysis was performed by inductively coupled plasma-mass spectrometry (ICP-MS).</p><p><strong>Results: </strong>Here, we demonstrate that SuperFifty increases the fruit size of both raspberries and strawberries and enhances the yield in these two berry crops by 42.1% (raspberry) and 33.9% (strawberry) while preserving the nutritional properties of the fruits. Metabolome analysis of 100 metabolites revealed that antioxidants, essential amino acids, organic acids, sugars, and vitamins, such as glutathione, alanine, asparagine, histidine, threonine, serine, tryptophan, sucrose, citric acid, pantothenic acid (vitamin B5), as well as other primary metabolites, remain the same in the SuperFifty-primed fruits. Secondary metabolites, such as caffeic acid, p-coumaric acid, kaempferol, and quercetin, also maintained their levels in the SuperFifty-primed fruits. Analysis of essential elements demonstrated that elements important for human health, such as Zn, Mn, Fe, B, Cu, K, and Ca, maintain the same levels in the raspberry and strawberry fruits obtained from the biostimulant-primed plants. Magnesium, an important element known as a co-factor in many enzymatic reactions related to both plant physiology and human health, increased in both raspberry and strawberry fruits primed with SuperFifty. Finally, we discuss the potential financial and health benefits of the SuperFifty-induced priming for both growers and consumers.</p><p><strong>Conclusions: </strong>We demonstrate that SuperFifty significantly enhances the yield of both raspberries and strawberries, improves the marketable grade of the fruits (larger and heavier fruits), and enhances the nutritional properties by elevating Mg content in the fruits. Altogether, this biostimulant-induced molecular priming offers an environmentally friendly, efficient, and sustainable way to enhance the yield and quality of berry crops, with clear benefits to both berry producers and customers.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aging and Pathological Conditions Similarity Revealed by Meta-Analysis of Metabolomics Studies Suggests the Existence of the Health and Age-Related Metapathway.","authors":"Petr G Lokhov, Elena E Balashova, Dmitry L Maslov, Oxana P Trifonova, Alexander I Archakov","doi":"10.3390/metabo14110593","DOIUrl":"10.3390/metabo14110593","url":null,"abstract":"<p><p><b>Background</b>: The incidence of many diseases increases with age and leads to multimorbidity, characterized by the presence of multiple diseases in old age. This phenomenon is closely related to systemic metabolic changes; the most suitable way to study it is through metabolomics. The use of accumulated metabolomic data to characterize this phenomenon at the system level may provide additional insight into the nature and strength of aging-disease relationships. <b>Methods</b>: For this purpose, metabolic changes associated with human aging and metabolic alterations under different pathological conditions were compared. To do this, the published results of metabolomic studies on human aging were compared with data on metabolite alterations collected in the human metabolome database through metabolite set enrichment analysis (MSEA) and combinatorial analysis. <b>Results</b>: It was found that human aging and pathological conditions involve the set of the same metabolic pathways with a probability of 99.96%. These data show the high identity of the aging process and the development of diseases at the metabolic level and allow to identify the set of metabolic pathways reflecting age-related changes closely associated with health. Based on these pathways, a metapathway was compiled, changes in which are simultaneously associated with health and age. <b>Conclusions</b>: The knowledge about the strength of the convergence of aging and pathological conditions has been supplemented by the rigor evidence at the metabolome level, which also made it possible to outline the age and health-relevant place in the human metabolism.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11597009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Effects of Loquat Juice (<i>Eriobotrya japonica</i> Lindl <i>Mkarkeb</i> Variety) on Lipid Homeostasis, Liver Steatosis, and Oxidative Stress in Hyperlipidemic Mice Fed a High-Fat-High-Fructose Diet.","authors":"Imane Mokhtari, Thamer Aljutaily, Huda Aljumayi, Khadija S Radhi, Abdulkarim S Almutairi, Hassan Barakat, Ibrahim Khalifa, Souliman Amrani, Hicham Harnafi","doi":"10.3390/metabo14110592","DOIUrl":"10.3390/metabo14110592","url":null,"abstract":"<p><strong>Background: </strong>Loquat fruit is consumed for its flavorful taste and a rich array of health-promoting compounds like phenolics, flavonoids, and carotenoids. This study aimed at the biochemical characterization of fresh juice from the Moroccan <i>Mkarkeb</i> variety of loquat and evaluating its effects on lipid homeostasis and liver steatosis in hyperlipidemic mice.</p><p><strong>Methods: </strong>The biochemical characterization followed AOAC methods. In vivo study involved hyperlipidemic mice fed a high-fat, high-fructose diet for 6 weeks and treated with loquat juice at 3.5 and 7 mL kg^-1 or fenofibrate at 4 mg·kg^-1. The concentrations of lipids in plasma, liver, adipose tissue, feces, and bile and blood glucose levels were quantified. Liver steatosis was visually examined and confirmed histologically, and liver injury markers (AST, ALT, ALP, LDH, and TB) were measured. Liver oxidative stress was assessed by measuring MDA content and antioxidative enzyme activities.</p><p><strong>Results: </strong>Our findings indicate that fresh loquat juice is poor in fat and protein and contains moderate sugars with a low energy value (40.82 ± 0.25 kcal/100 g). It is also rich in minerals, vitamin C, phenolic acids, flavonoids, and carotenoids. The juice effectively restored lipid metabolism by enhancing reverse cholesterol transport and lowering LDL-cholesterol, triglycerides, and the atherogenic index. The studied juice decreases blood glucose and prevents weight gain and lipid accumulation in the liver and adipose tissue. The juice prevents lipotoxicity-induced liver injury, corrects toxicity markers, and improves the liver's morphological and histological structures. It also reduces oxidative stress by lowering MDA and activating SOD and catalase.</p><p><strong>Conclusions: </strong>The juice holds high nutritional and medicinal value, potentially preventing lipid disorders and cardiovascular issues.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Metabolomic and Transcriptomic Analysis of <i>Nitraria</i> Berries Indicate the Role of Flavonoids in Adaptation to High Altitude.","authors":"Qing Zhao, Jie Zhang, Yanhong Li, Zufan Yang, Qian Wang, Qiangqiang Jia","doi":"10.3390/metabo14110591","DOIUrl":"10.3390/metabo14110591","url":null,"abstract":"<p><p><b>Background:</b> Plants of <i>Nitraria</i>, belonging to the Zygophyllaceae family, are not only widely distributed at an altitude of about 1000 m but also at an altitude of about 3000 m, which is a rare phenomenon. However, little is known about the effect of altitude on the accumulation of metabolites in plants of <i>Nitraria</i>. Furthermore, the mechanism of the high-altitude adaptation of <i>Nitraria</i> has yet to be fully elucidated. <b>Methods:</b> In this study, metabolomics and transcriptomics were used to investigate the differential accumulation of metabolites of <i>Nitraria</i> berries and the regulatory mechanisms in different altitudes. <b>Results:</b> As a result, the biosynthesis of flavonoids is the most significant metabolic pathway in the process of adaptation to high altitude, and 5 Cyanidins, 1 Pelargonidin, 3 Petunidins, 1 Peonidin, and 4 Delphinidins are highly accumulated in high-altitude <i>Nitraria</i>. The results of transcriptomics showed that the structural genes <i>C</i>4<i>H</i> (2), <i>F</i>3<i>H</i>, 4<i>CL</i> (2), <i>DFR</i> (2), <i>UFGT</i> (2), and <i>FLS</i> (2) were highly expressed in high-altitude <i>Nitraria</i>. A network metabolism map of flavonoids was constructed, and the accumulation of differential metabolites and the expression of structural genes were analyzed for correlation. <b>Conclusions:</b> In summary, this study preliminarily offers a new understanding of metabolic differences and regulation mechanisms in plants of <i>Nitraria</i> from different altitudes.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596137/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Identification of Alkaloid Genes from the Biomass of <i>Fritillaria taipaiensis</i> P.Y. Li.","authors":"Nong Zhou, Chun-Mei Mei, Fu-Gui Chen, Yu-Wei Zhao, Ming-Guo Ma, Wei-Dong Li","doi":"10.3390/metabo14110590","DOIUrl":"10.3390/metabo14110590","url":null,"abstract":"<p><strong>Background/objectives: </strong><i>Fritillaria taipaiensis</i> P.Y. Li is a valuable traditional Chinese medicinal herb that utilizes bulbs as medicine, which contain multiple alkaloids. Biomass, as a sustainable resource, has promising applications in energy, environmental, and biomedical fields. Recently, the biosynthesis and regulatory mechanisms of the main biomass components of biomass have become a prominent research topic.</p><p><strong>Methods: </strong>In this article, we explored the differences in the heterosteroidal alkaloid components of <i>F. taipaiensis</i> biomass using liquid chromatography-mass spectrometry and high-throughput transcriptome sequencing.</p><p><strong>Results: </strong>The experimental results demonstrated significant differences in the eight types of heterosteroidal alkaloid components among the biomass of <i>F. taipaiensis</i>, including peimisine, imperialine, peimine, peiminine, ebeinone, ebeiedine, ebeiedinone, and forticine. Transcriptomic analysis revealed substantial significant differences in gene expression patterns in the various samples. Three catalytic enzyme-coding genes, 3-hydroxy-3-methylglutaryl coenzyme A synthase (<i>HMGS</i>), 3-hydroxy-3-methylglutaryl coenzyme A reductase (<i>HMGR</i>), and terpene synthase (<i>TPS</i>), were speculated to contribute to the regulation of the differential accumulation of alkaloid synthesis in <i>F. taipaiensis</i> bulbs. A strong positive correlation was observed between the transcriptional level of the <i>TPS</i> gene and the alkaloid content of <i>F. taipaiensis</i> biomass, suggesting that <i>TPS</i> may be a key gene in the biosynthesis pathway of alkaloids. This finding can be used for subsequent gene function verification and molecular regulatory network analysis.</p><p><strong>Conclusions: </strong>This work provides fundamental data and novel insights for the subsequent research on alkaloid biosynthesis in <i>F. taipaiensis</i>.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exercise as a Therapeutic Strategy for Obesity: Central and Peripheral Mechanisms.","authors":"Yiyin Zhang, Ruwen Wang, Tiemin Liu, Ru Wang","doi":"10.3390/metabo14110589","DOIUrl":"10.3390/metabo14110589","url":null,"abstract":"<p><p>Obesity is a complex, multifactorial condition involving excessive fat accumulation due to an imbalance between energy intake and expenditure, with its global prevalence steadily rising. This condition significantly increases the risk of chronic diseases, including sarcopenia, type 2 diabetes, and cardiovascular diseases, highlighting the need for effective interventions. Exercise has emerged as a potent non-pharmacological approach to combat obesity, targeting both central and peripheral mechanisms that regulate metabolism, energy expenditure, and neurological functions. In the central nervous system, exercise influences appetite, mood, and cognitive functions by modulating the reward system and regulating appetite-controlling hormones to manage energy intake. Concurrently, exercise promotes thermogenesis in adipose tissue and regulates endocrine path-ways and key metabolic organs, such as skeletal muscle and the liver, to enhance fat oxidation and support energy balance. Despite advances in understanding exercise's role in obesity, the precise interaction between the neurobiological and peripheral metabolic pathways remains underexplored, particularly in public health strategies. A better understanding of these interactions could inform more comprehensive obesity management approaches by addressing both central nervous system influences on behavior and peripheral metabolic regulation. This review synthesizes recent insights into these roles, highlighting potential therapeutic strategies targeting both systems for more effective obesity interventions.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bupropion Increased More than Five Times the Systemic Exposure to Aripiprazole: An In Vivo Study in <i>Wistar albino</i> Rats.","authors":"Iulia-Maria Ciocotișan, Dana Maria Muntean, Laurian Vlase","doi":"10.3390/metabo14110588","DOIUrl":"10.3390/metabo14110588","url":null,"abstract":"<p><p><b>Background/Objectives</b>: In psychiatric disorders, antipsychotics and antidepressant medication are often administered together. Aripiprazole, a third-generation antipsychotic drug, is extensively metabolized by CYP2D6 and CYP3A4 isoenzymes, while bupropion, used in depressive disorders, is known as a moderate or strong CYP2D6 enzyme inhibitor. This in vivo experiment aimed to assess the presence of a pharmacokinetic drug interaction between aripiprazole and bupropion and its magnitude on the systemic exposure of aripiprazole. <b>Methods</b>: 24 healthy <i>Wistar albino</i> male rats were included in two study groups. A single dose of 8 mg/kg aripiprazole was given to rats in the reference group, while the test group received repeated doses of bupropion for 6 days, followed by a single dose of aripiprazole. An LC-MS/MS method was developed for the concomitant quantification of aripiprazole and its active metabolite, dehydroaripiprazole, and non-compartmental analysis was employed to assess their pharmacokinetic parameters. <b>Results</b>: The mean AUC_0-∞ of aripiprazole increased 5.65-fold (1117.34 ± 931.41 vs. 6311.66 ± 2978.71 hr·ng/mL), the mean C_max increased by 96.76% and the apparent systemic clearance decreased over 9-fold after bupropion repeated doses. The exposure to aripiprazole's active metabolite increased as well, having a 4-fold increase in the mean AUC_0-∞ (from 461.13 ± 339.82 to 1878.66 ± 1446.91 hr·ng/mL) and a 2-fold increase in the mean C_max. <b>Conclusions</b>: The total exposure to the aripiprazole parent compound and active moiety significantly increased after bupropion pretreatment in this preclinical in vivo experiment. Clinical studies should further establish the significance of this interaction in humans.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Online Direct Infusion Mass Spectrometry of Liquid-Liquid Extraction Phases for Metabolite and Lipid Profiling with the Direct Infusion Probe.","authors":"Cátia Marques, Lena Blaase, Ingela Lanekoff","doi":"10.3390/metabo14110587","DOIUrl":"10.3390/metabo14110587","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Profiling of metabolites and lipids in biological samples can provide invaluable insights into life-sustaining chemical processes. The ability to detect both metabolites and lipids in the same sample can enhance these understandings and connect cellular dynamics. However, simultaneous detection of metabolites and lipids is generally hampered by chromatographic systems tailored to one molecular type. This void can be filled by direct infusion mass spectrometry (MS), where all ionizable molecules can be detected simultaneously. However, in direct infusion MS, the high chemical complexity of biological samples can introduce limitations in detectability due to matrix effects causing ionization suppression. <b>Methods:</b> Decreased sample complexity and increased detectability and molecular coverage was provided by combining our direct infusion probe (DIP) with liquid-liquid extraction (LLE) and directly sampling the different phases for direct infusion. Three commonly used LLE methods for separating lipids and metabolites were evaluated. <b>Results:</b> The butanol-methanol (BUME) method was found to be preferred since it provides high molecular coverage and have low solvent toxicity. The established BUME DIP-MS method was used as a fast and sensitive analysis tool to study chemical changes in insulin-secreting cells upon glucose stimulation. By analyzing the metabolome at distinct time points, down to 1-min apart, we found high dynamics of the intracellular metabolome. <b>Conclusions:</b> The rapid workflow with LLE DIP-MS enables higher sensitivity of phase separated metabolites and lipids. The application of BUME DIP-MS provides novel information on the dynamics of the intracellular metabolome of INS-1 during the two phases of insulin release for both metabolite and lipid classes.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Irisin, Myostatin, and Myonectin Correlate with Metabolic Health Markers, Liver Disease Progression, and Blood Pressure in Patients with Metabolic Dysfunction-Associated Fatty Liver Disease and Hypertension.","authors":"Anna F Sheptulina, Elvira M Mamutova, Anastasia Yu Elkina, Yuriy S Timofeev, Victoria A Metelskaya, Anton R Kiselev, Oxana M Drapkina","doi":"10.3390/metabo14110584","DOIUrl":"10.3390/metabo14110584","url":null,"abstract":"<p><strong>Background/objectives: </strong>Recent data indicate the involvement of skeletal muscles in the regulation of metabolism and in the pathogenesis of chronic noncommunicable diseases. The goal of our study was to describe the serum concentrations of myokines in patients with metabolic dysfunction-associated steatotic liver disease (MASLD) and hypertension (HTN) and their correlation with laboratory parameters, blood pressure (BP), and MASLD severity.</p><p><strong>Methods: </strong>A total of 67 patients with MASLD and HTN underwent anthropometric measurements, laboratory tests, and point shear-wave elastography. The serum concentrations of myokines were measured using enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>Patients with detectable serum myonectin concentrations had significantly higher maximum systolic blood pressure (<i>p</i> = 0.022) and higher blood levels of uric acid (<i>p</i> = 0.029). Serum irisin concentration ≥ 6.1 μg/mL was associated with higher FLI values (<i>p</i> = 0.042) and liver stiffness (<i>p</i> = 0.034), as well as with slightly higher waist circumference (<i>p</i> = 0.082) and triglyceride level (<i>p</i> = 0.062). Patients with serum myostatin concentration ≥ 4.98 ng/mL were significantly older (<i>p</i> = 0.033) and had a lower blood albumin level (<i>p</i> = 0.043).</p><p><strong>Conclusions: </strong>In conclusion, the myokine profile in patients with MASLD and HTN correlates both with the severity of MASLD and the parameters characteristic of metabolic health, suggesting the possible contribution of altered irisin, myonectin, and myostatin concentrations to the occurrence of cardiometabolic risks in patients with MASLD.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}