Metabolites最新文献

{"title":"Argan Fruit Polyphenols Regulate Lipid Homeostasis, Prevent Liver Fat Accumulation, and Improve Antioxidant Defense in High-Calorie Diet Fed Mice: In Vivo Study and In Silico Prediction of Possible Underlying Mechanisms.","authors":"Mohammadine Moumou, Imane Mokhtari, Mohamed Harnafi, Mohammed Alrugaibah, Thamer Aljutaily, Hend F Alharbi, Abdulmalik Alhuwaymil, Abdulkarim S Almutairi, Hassan Barakat, Dragan Milenkovic, Souliman Amrani, Hicham Harnafi","doi":"10.3390/metabo15040234","DOIUrl":"https://doi.org/10.3390/metabo15040234","url":null,"abstract":"<p><p><b>Background/Objectives:</b><i>Argania spinosa</i> L. Skeels is a Moroccan endemic plant widely used by the local population as folk medicine. This study aimed to investigate the effects of Argan fruit pulp on lipid metabolism disorders and liver steatosis in hypercaloric diet-fed mice. <b>Methods:</b> Animals were treated with the Argan fruit pulp extract and its fractions for 12 weeks at 100 and 200 mg Kg^-1 BW daily. The analysis was conducted on lipid levels in plasma, liver, feces, and bile as well as on glycemia. The liver glutathione, malondialdehyde, and antioxidant enzyme activities were assessed. The hepatic steatosis was evaluated by measuring transaminases and alkaline phosphatase activities and examining histological sections. The polyphenol profiles were determined using HPLC-DAD. Possible underlying mechanisms in the hypolipidemic and hepatoprotective activities were predicted by molecular docking. <b>Results:</b> The crude extract and its aqueous fraction (rich in protocatechuic and gallic acids) significantly restored plasma lipids and glucose levels. Indeed, total cholesterol level (TCHO) was decreased in the liver but increased in bile and feces. The treatment also reduced body weight and liver and adipose tissue mass and prevented liver steatosis. The ethyl acetate fraction exhibited no effect on lipid metabolism but significantly prevented liver oxidative stress. The crude extract and its fractions appear to be nontoxic (LD50 > 5000 mg Kg^-1) in mice. The phenolic acids demonstrated strong binding affinity to key targets involved in regulating lipid homeostasis, including ABCA-1, LXR, CYP7A1, HMH-CoA reductase, and PCSK-9. However, the identified flavonoids exhibited high affinities to targets involved in oxidative stress defense (SOD, CAT, and CYP2E1). <b>Conclusions:</b> The Argan fruit pulp, particularly its polyphenols, could be a promising natural approach for preventing cardio-metabolic diseases by improving lipid metabolism and reducing liver oxidative stress.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Pretreatment Methods and Detection Techniques for Veterinary Drug Residues in Animal-Derived Foods.","authors":"Qing Dai, Shusheng Tang, Chongshan Dai","doi":"10.3390/metabo15040233","DOIUrl":"https://doi.org/10.3390/metabo15040233","url":null,"abstract":"<p><p>Veterinary drugs are extensively employed in livestock, poultry, and aquaculture, playing a crucial role in preventing and treating animal diseases, facilitating growth, and enhancing feed conversion rates. Nevertheless, veterinary drug residues in animal-derived foods pose a direct or potential threat to human life and health. Precise detection of these residues in animal-derived foods to ensure their safety has become an important mission. In this review, we sum up the current progress of applied pretreatment methods and detection techniques for veterinary drug residues in animal-derived foods. At present, sample pretreatment methods mainly consist of the following: liquid-liquid extraction; solid-phase extraction; immunoaffinity chromatography; Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method; and molecular imprinting technology. Detection techniques mainly involve chromatographic techniques, immunoassay techniques, fluorescence polarization immunoassay, and surface-enhanced Raman scattering. We also discussed the advantages and limitations of these technologies. Moreover, we point out the development direction and tendency of detection techniques in the future, providing references for the detection of veterinary drug residues in animal-derived foods.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormonal Crossroads in Inborn Errors of the Metabolism Impact of Puberty and Dietary Interventions on Metabolic Health.","authors":"Thomas Lundqvist, Rasmus Stenlid, Maria Halldin","doi":"10.3390/metabo15040235","DOIUrl":"https://doi.org/10.3390/metabo15040235","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Inborn errors of metabolism (IEMs) represent a diverse group of genetic disorders characterized by enzymatic defects that disrupt metabolic pathways, leading to toxic metabolite accumulation, deficits, or impaired macromolecule synthesis. While strict dietary interventions are critical for managing many of these conditions, hormonal and metabolic changes during puberty introduce new challenges. Advancements in early diagnosis and treatment have significantly extended the lifespan of individuals with IEMs. However, this increased longevity is associated with heightened risks of new medical problems, including obesity, insulin resistance, and type 2 diabetes mellitus (T2DM), as these complications share mechanistic features with those seen in obesity and T2DM. <b>Methods</b>: This mini-review examines current knowledge of the intricate interplay between pubertal hormones and metabolic pathways in IEM patients. <b>Results</b>: We address critical questions, such as if puberty intensifies the risk of metabolic derangements in these individuals and if there is a metabolic intersection where these disorders converge, leading to shared complications. We highlight the impact of puberty-induced hormonal fluctuations, such as growth hormone (GH) surges and sex steroid activity, on disorders like phenylketonuria, urea cycle defects, and fatty acid oxidation disorders. Moreover, we explore the role of dietary interventions in mitigating or exacerbating these effects, emphasizing the importance of balancing nutritional needs during growth spurts. <b>Conclusions</b>: A multidisciplinary approach integrating endocrinology, nutrition, and emerging therapies is advocated to optimize metabolic health during puberty. Addressing these challenges is critical for improving long-term outcomes for individuals with IEMs, particularly during this pivotal developmental phase.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-Free DNA (cfDNA) Regulates Metabolic Remodeling, Sustaining Proliferation, Quiescence, and Migration in MDA-MB-231, a Triple-Negative Breast Carcinoma (TNBC) Cell Line.","authors":"Isabel Lemos, Catarina Freitas-Dias, Ana Hipólito, José Ramalho, Fabrizio Carteni, Luís G Gonçalves, Stefano Mazzoleni, Jacinta Serpa","doi":"10.3390/metabo15040227","DOIUrl":"https://doi.org/10.3390/metabo15040227","url":null,"abstract":"<p><p><b>Background:</b> The clinical relevance of circulating cell-free DNA (cfDNA) in oncology has gained significant attention, with its potential as a biomarker for cancer diagnosis and monitoring. However, its precise role in cancer biology and progression remains unclear. cfDNA in cancer patients' blood has been shown to activate signaling pathways, such as those mediated by toll-like receptors (TLRs), suggesting its involvement in cancer cell adaptation to the tumor microenvironment. <b>Methods:</b> This impact of cfDNA released from MDA-MB-231, a triple-negative breast cancer (TNBC) cell line was assessed, focusing on glucose availability and culture duration. The impact of cfDNA on the proliferation of MDA-MB-231 cells was investigated using proliferation curves, while cellular migration was evaluated through wound healing assays. The metabolic alterations induced by distinct cfDNA variants in MDA-MB-231 cells were investigated through nuclear magnetic resonance (NMR) spectroscopy, and their effect on cisplatin resistance was evaluated using flow cytometry. Furthermore, the expression levels of DNA-sensitive Toll-like receptor 9 (TLR9) were quantified via immunofluorescence, alongside its colocalization with lysosome-associated membrane protein 1 (LAMP1). <b>Results:</b> This study indicates that cfDNA facilitates metabolic adaptation, particularly under metabolic stress, by modulating glucose and glutamine consumption, key pathways in tumor cell metabolism. Exposure to cfDNA induced distinct metabolic shifts, favoring energy production through oxidative phosphorylation. The anti-cancer activity of cfDNA isolated from conditioned media of cells cultured under stressful conditions is influenced by the culture duration, emphasizing the importance of adaptation and se-lection in releasing cfDNA that can drive pro-tumoral processes. Additionally, cfDNA exposure influenced cell proliferation, quiescence, and migration, processes linked to metastasis and treatment resistance. These findings underscore cfDNA as a key mediator of metabolic reprogramming and adaptive responses in cancer cells, contributing to tumor progression and therapy resistance. Furthermore, the activation of TLR9 signaling suggests a mechanistic basis for cfDNA-induced phenotypic changes. <b>Conclusions</b>: Overall, cfDNA serves as a crucial signaling molecule in the tumor microenvironment, orchestrating adaptive processes that enhance cancer cell survival and progression.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Tea Polyphenols Alleviate Acute-Heat-Stress-Induced Death of Hybrid Crucian Carp HCC2: Involvement of Modified Lipid Metabolisms in Liver.","authors":"Na Zhang, Jinsheng Tao, Qifang Yu, Gege Sun, Xiaopeng Liu, Weirong Tang, Lina Zhang, Zhe Yang","doi":"10.3390/metabo15040229","DOIUrl":"https://doi.org/10.3390/metabo15040229","url":null,"abstract":"<p><strong>Background: </strong>Global warming poses significant challenges to aquaculture, as elevated water temperatures adversely affect fish health and survival. This study investigated the effects and potential mechanisms of dietary tea polyphenols (TPs) on acute heat stress and survival in hybrid crucian carp HCC2.</p><p><strong>Methods: </strong>The fish in the control (CON) group and heat stress group (HS group, three replicates, each containing 20 fish, <i>n</i> = 60 per group) were fed diets with 0 mg/kg TPs, and the three experimental groups (HSLTP, HSMTP, and HSHTP, <i>n</i> = 20 × 3 replicates) were fed the diets with 100, 200, or 400 mg/kg TPs for 60 days. Further, fish in the experimental groups (HS, HSLTP, HSMTP, and HSHTP) were exposed at 38 °C for 24 h to induce acute heat stress. Survival data and serum and tissue samples were collected for the analysis. Metabolomics using UPLC-Q-TOF/MS was employed to evaluate the metabolite changes in the fish livers.</p><p><strong>Results: </strong>Notably, dietary TPs significantly improved survival rates and antioxidant enzyme levels and reduced serum ALT, AST, cortisol, glucose, MDA, and liver HSP-70 levels in the heat-stressed fish. Metabolomic analysis revealed that TPs modulated lipid metabolism, particularly glycerophospholipid and arachidonic acid pathways, which may contribute to a higher tolerance to acute heat stress.</p><p><strong>Conclusions: </strong>These findings suggest that TPs are a promising, eco-friendly feed additive for protecting fish from heat stress and optimizing aquaculture practices.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12028923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Profiling Reveals Potential Prognostic Biomarkers for SFTS: Insights into Disease Severity and Clinical Outcomes.","authors":"Zhuo-Min Zhu, Huan-Yu Liu, Na An, An-Ling Li, Jia Li, Sai-Jun Wang, Gui Yang, Yong-Wei Duan, Ying Yang, Mei Zhang, Quan-Fei Zhu, Song-Mei Liu, Yu-Qi Feng","doi":"10.3390/metabo15040228","DOIUrl":"https://doi.org/10.3390/metabo15040228","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Severe fever with thrombocytopenia syndrome (SFTS) is a viral infection primarily found in Asia, with a case fatality rate of about 10%. Despite its increasing prevalence, the underlying pathogenic mechanisms remain poorly understood, limiting the development of effective therapeutic interventions. <b>Methods:</b> We employed an untargeted metabolomics approach using liquid chromatography-mass spectrometry (LC-MS) to analyze serum samples from 78 SFTS patients during the acute phase of their illness. Differential metabolic features between survival and fatal cases were identified through multivariate statistical analysis. Furthermore, we constructed a metabolic prognostic model based on these biomarkers to predict disease severity. <b>Results:</b> Significant alterations were observed in four key metabolic pathways: sphingolipid metabolism, biosynthesis of phenylalanine, tyrosine, and tryptophan, primary bile acid biosynthesis, and phenylalanine metabolism. Elevated levels of phenyllactic acid and isocitric acid were strongly associated with adverse outcomes and demonstrated high discriminatory power in distinguishing fatal cases from survivors. The metabolic prognostic model incorporating these biomarkers achieved a sensitivity of 75% and a specificity of 90% in predicting disease severity. <b>Conclusions:</b> Our findings highlight the pivotal role of metabolic dysregulation in the pathogenesis of SFTS and suggest that targeting specific metabolic pathways could open new avenues for therapeutic development. The identification of prognostic biomarkers provides a valuable tool for early risk stratification and timely clinical intervention, potentially improving patient outcomes.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12028903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adiponectin and All-Cause Mortality in Patients with Chronic Kidney Disease: A Systematic Review and Meta-Analysis.","authors":"Hyun Suk Yang, Soo-Nyung Kim, Jung-Hoon Ro, Mina Hur","doi":"10.3390/metabo15040230","DOIUrl":"https://doi.org/10.3390/metabo15040230","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Elevated levels of adiponectin in chronic kidney disease (CKD) have been paradoxically associated with increased mortality. This meta-analysis aimed to evaluate the association between circulating adiponectin levels and all-cause mortality in patients with CKD, in total and various subgroups. <b>Methods</b>: We systematically searched PubMed, Embase, and Cochrane Library from their inception to December 2024 for studies examining baseline adiponectin levels and observed mortality outcomes in patients with CKD. Studies were included if they evaluated CKD stages 2-5 patients, measured baseline circulating adiponectin levels, and reported hazard ratios (HRs) for all-cause mortality. We excluded non-original research, studies of acute conditions, normal kidney function, kidney transplantation, and those using log-transformed or standardized HRs. HRs with a 95% confidence interval (CI) for all-cause mortality risk per 1 µg/mL increase in adiponectin were extracted and analyzed using the Comprehensive Meta-Analysis Version 4. Study quality was assessed using the Newcastle-Ottawa Scale. <b>Results</b>: Twelve studies with 2523 subjects were included. The pooled unadjusted HR was 1.003 (95% CI: 0.981-1.025) using a random-effects model (<i>I</i>² = 79%). Subgroup analyses demonstrated increased mortality risk with elevated adiponectin levels in non-Asia (HR 1.021 [95% CI: 1.006-1.037], <i>p</i> = 0.006), studies with female proportion <47% (HR 1.021 [95% CI: 1.009-1.033], <i>p</i> < 0.001), and studies with body mass index ≥25 kg/m² (HR 1.023 [95% CI: 1.008-1.038], <i>p</i> = 0.003). In contrast, higher adiponectin levels were associated with decreased mortality risk in the peritoneal dialysis group (HR 0.956 [95% CI: 0.934-0.979], <i>p</i> < 0.001) and female proportion ≥47% group (HR 0.929 [95% CI: 0.874-0.988], <i>p</i> = 0.019). <b>Discussion/Conclusions</b>: This meta-analysis revealed that elevated adiponectin levels have varying associations with the risk of all-cause mortality across CKD patient subgroups. These findings suggest that the prognostic value of adiponectin levels in CKD may be modulated by demographic and clinical factors. Limitations include poor generalizability with underrepresentation of early-stage CKD. This research received no external funding and was not registered.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12028947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolome Profiling and Predictive Modeling of Dark Green Leaf Trait in Bunching Onion Varieties.","authors":"Tetsuya Nakajima, Mari Kobayashi, Masato Fuji, Kouei Fujii, Mostafa Abdelrahman, Yasumasa Matsuoka, Jun'ichi Mano, Muneo Sato, Masami Yokota Hirai, Naoki Yamauchi, Masayoshi Shigyo","doi":"10.3390/metabo15040226","DOIUrl":"https://doi.org/10.3390/metabo15040226","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Background:&lt;/b&gt; The dark green coloration of bunching onion leaf blades is a key determinant of market value, nutritional quality, and visual appeal. This trait is regulated by a complex network of pigment interactions, which not only determine coloration but also serve as critical indicators of plant growth dynamics and stress responses. This study aimed to elucidate the mechanisms regulating the dark green trait and develop a predictive model for accurately assessing pigment composition. These advancements enable the efficient selection of dark green varieties and facilitate the establishment of optimal growth environments through plant growth monitoring. &lt;b&gt;Methods:&lt;/b&gt; Seven varieties and lines of heat-tolerant bunching onions were analyzed, including two commercial F1 cultivars, along with two purebred varieties and three F1 hybrid lines bred in Yamaguchi Prefecture. The analysis was conducted on visible spectral reflectance data (400-700 nm at 20 nm intervals) and pigment compounds (chlorophyll &lt;i&gt;a&lt;/i&gt;, chlorophyll &lt;i&gt;b&lt;/i&gt; and pheophytin &lt;i&gt;a&lt;/i&gt;, lutein, and β-carotene), whereas primary and secondary metabolites were assessed by using widely targeted metabolomics. In addition, a random forest regression model was constructed by using spectral reflectance data and pigment compound contents. &lt;b&gt;Results:&lt;/b&gt; Principal component analysis based on spectral reflectance data and the comparative profiling of 186 metabolites revealed characteristic metabolite accumulation associated with each green color pattern. The \"green\" group showed greater accumulation of sugars, the \"gray green\" group was characterized by the accumulation of phenolic compounds, and the \"dark green\" group exhibited accumulation of cyanidins. These metabolites are suggested to accumulate in response to environmental stress, and these differences are likely to influence green coloration traits. Furthermore, among the regression models for estimating pigment compound contents, the one for chlorophyll &lt;i&gt;a&lt;/i&gt; content achieved high accuracy, with an R2 value of 0.88 in the test dataset and 0.78 in Leave-One-Out Cross-Validation, demonstrating its potential for practical application in trait evaluation. However, since the regression model developed in this study is based on data obtained from greenhouse conditions, it is necessary to incorporate field trial results and reconstruct the model to enhance its adaptability. &lt;b&gt;Conclusions:&lt;/b&gt; This study revealed that cyanidin is involved in the characteristics of dark green varieties. Additionally, it was demonstrated that chlorophyll &lt;i&gt;a&lt;/i&gt; can be predicted using visible spectral reflectance. These findings suggest the potential for developing markers for the dark green trait, selecting high-pigment-accumulating varieties, and facilitating the simple real-time diagnosis of plant growth conditions and stress status, thereby enabling the establishment of optimal environmental conditions. Future studies will aim to elucidat","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12028952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling and Discrimination of Euryale Ferox Seeds from Different Processing Methods Using Liquid Chromatography High-Resolution Mass Spectrometry Combined with Molecular Networking and Statistical Analysis.","authors":"Xiaoyu Xie, Chuntao Zeng, Ruonan Zhang, Wenting Zhu, Huijie Li, Zhi Huang","doi":"10.3390/metabo15040225","DOIUrl":"https://doi.org/10.3390/metabo15040225","url":null,"abstract":"<p><strong>Background: </strong>Euryale ferox seeds (EFSs) serve both medicinal and culinary purposes. They possess high nutritional value and are rich in polysaccharides, polyphenols, glycolipids, cyclic peptides, and other beneficial components. EFSs are known for their effects in tonifying the kidneys and strengthening essence, invigorating the spleen and alleviating diarrhea, as well as removing dampness and leucorrhea. Processing can alter the chemical composition of EFSs, with different methods yielding varying effects on their chemical makeup and, consequently, their efficacy. However, to date, no studies have systematically investigated the overall chemical composition of EFSs using different processing methods.</p><p><strong>Methods: </strong>In this study, we employed liquid chromatography high-resolution mass spectrometry (LC-HRMS) to identify the compounds in EFSs by searching databases and Global Natural Products Social Molecular Networking (GNPS), and we comprehensively explored the changes in the chemical composition of EFSs resulting from various processing methods via statistical analysis.</p><p><strong>Results: </strong>A total of 438 compounds were identified from EFSs, of which 283 were identified through database searches and 155 were identified via GNPS propagation. Statistical analysis revealed 32 and 38 differential compounds in dry-fried Euryale ferox seeds (DFEFSs) and bran-fried Euryale ferox seeds (BFEFSs), respectively. Additionally, we found a significant increase in the lipid content of the fried EFSs.</p><p><strong>Conclusions: </strong>This study provides valuable data to support the quality evaluation of processed EFSs and contributes to the research on the material basis of their medicinal efficacy.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12028916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Extraction Method: Microwave-Assisted Water Extraction Followed by HILIC-HRMS Analysis to Quantify Hydrophilic Compounds in Plants.","authors":"Alexandra Louis, Jean François Chich, Hadrien Chepca, Isabelle Schmitz, Philippe Hugueney, Alessandra Maia-Grondard","doi":"10.3390/metabo15040223","DOIUrl":"https://doi.org/10.3390/metabo15040223","url":null,"abstract":"<p><p><b>Background:</b> Hydrophilic compounds, such as amino acids, organic acids and sugars, among others, are present in large amounts in plant cells. The analysis and quantification of these major hydrophilic compounds are particularly relevant in plant science because they have a considerable impact on the quality of plant-derived products and on plant-pathogen relationships. Our objective was to develop and validate a complete analysis workflow combining a water-based extraction procedure with a fast separation using hydrophilic interaction liquid chromatography coupled to high-resolution mass spectrometry (HILIC-HRMS) for quantitative analysis of hydrophilic compounds in plant tissues. <b>Results:</b> Water-based microwave-assisted extraction (MAE) methods for hydrophilic compounds were compared using HILIC-HRMS. The newly developed method involved 20 s MAE time followed by a 10 min HILIC-HRMS analysis. This bioanalytical method was validated for 24 polar metabolites, including amino acids, organic acids, and sugars, to ensure the reliability of analytical results: selectivity, limits of detection and quantification, calibration range and precision. Depending on the compounds, quantification limit was as low as 0.10 µM up to 4.50 µM. Between-run RSDs evaluated on <i>Vitis vinifera</i> and <i>Arabidopsis</i> samples were all below 20% except for three compounds. <b>Conclusions:</b> A water-based MAE method, coupled with HILIC-HRMS, was developed for the absolute quantification of free amino acids, organic acids, and sugars in plant tissues. Its effectiveness was demonstrated in both lignified plants, such as <i>Vitis vinifera</i>, and non-lignified plants, such as <i>Arabidopsis</i>. This method is suitable for medium- to high-throughput analysis of key polar metabolites from small amounts of plant material.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}