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Methods in molecular biology最新文献

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Production of Neuroepithelial Organoids from Human-Induced Pluripotent Stem Cells for Mimicking Early Neural Tube Development. 利用人体诱导多能干细胞生产神经上皮组织块，以模拟早期神经管发育。
Methods in molecular biology Pub Date : 2024-04-23 DOI: 10.1007/7651_2024_546
Chunling Tang, Xinghui Wang, Eileen Gentleman, Nicholas A Kurniawan
{"title":"Production of Neuroepithelial Organoids from Human-Induced Pluripotent Stem Cells for Mimicking Early Neural Tube Development.","authors":"Chunling Tang, Xinghui Wang, Eileen Gentleman, Nicholas A Kurniawan","doi":"10.1007/7651_2024_546","DOIUrl":"https://doi.org/10.1007/7651_2024_546","url":null,"abstract":"","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"11 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140666709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Measurement of Myonuclear Accretion In Vitro and In Vivo. 体内和体外肌核增殖测量
Methods in molecular biology Pub Date : 2024-04-23 DOI: 10.1007/7651_2024_540
Lola Lessard, Audrey Saugues, Julien Gondin, Rémi Mounier, Anita Kneppers
{"title":"Measurement of Myonuclear Accretion In Vitro and In Vivo.","authors":"Lola Lessard, Audrey Saugues, Julien Gondin, Rémi Mounier, Anita Kneppers","doi":"10.1007/7651_2024_540","DOIUrl":"https://doi.org/10.1007/7651_2024_540","url":null,"abstract":"","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"93 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140670145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Organoid Culture of Different Intestinal Segments from Human and Mouse. 人和小鼠不同肠段的类器官培养
Methods in molecular biology Pub Date : 2024-04-23 DOI: 10.1007/7651_2024_542
Yalong Wang, Ronghui Tan, Ye-Guang Chen
{"title":"Organoid Culture of Different Intestinal Segments from Human and Mouse.","authors":"Yalong Wang, Ronghui Tan, Ye-Guang Chen","doi":"10.1007/7651_2024_542","DOIUrl":"https://doi.org/10.1007/7651_2024_542","url":null,"abstract":"","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"62 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140668322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protocol for Testing the Effects of ssDNA Aptamer in HeLa and MCF-7. 在 HeLa 和 MCF-7 中测试 ssDNA Aptamer 效果的规程。
Methods in molecular biology Pub Date : 2024-04-19 DOI: 10.1007/7651_2024_539
Haregewoin Bezu Woldekidan, Zandile Nxumalo, Mutsa Takundwa, A. A. Woldesemayat, Deepak B. Thimiri Govinda Raj
{"title":"Protocol for Testing the Effects of ssDNA Aptamer in HeLa and MCF-7.","authors":"Haregewoin Bezu Woldekidan, Zandile Nxumalo, Mutsa Takundwa, A. A. Woldesemayat, Deepak B. Thimiri Govinda Raj","doi":"10.1007/7651_2024_539","DOIUrl":"https://doi.org/10.1007/7651_2024_539","url":null,"abstract":"","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" April","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140682570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Mitochondrial Phagy (Mitophagy) in Human Non-small Adenocarcinoma Tumor Cells. 评估人类非小腺癌肿瘤细胞中的线粒体吞噬作用（Mitophagy）。
Methods in molecular biology Pub Date : 2024-04-13 DOI: 10.1007/7651_2024_532
Javad Alizadeh, Simone C da Silva Rosa, Marco Cordani, Saeid Ghavami
{"title":"Evaluation of Mitochondrial Phagy (Mitophagy) in Human Non-small Adenocarcinoma Tumor Cells.","authors":"Javad Alizadeh, Simone C da Silva Rosa, Marco Cordani, Saeid Ghavami","doi":"10.1007/7651_2024_532","DOIUrl":"https://doi.org/10.1007/7651_2024_532","url":null,"abstract":"","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"24 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140707316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of Bitter Taste Receptor-Dependent Autophagy in Oral Epithelial Cells. 口腔上皮细胞中苦味受体依赖性自噬的特征。
Methods in molecular biology Pub Date : 2024-04-06 DOI: 10.1007/7651_2024_531
Nisha Singh, Saeid Ghavami, Prashen Chelikani
{"title":"Characterization of Bitter Taste Receptor-Dependent Autophagy in Oral Epithelial Cells.","authors":"Nisha Singh, Saeid Ghavami, Prashen Chelikani","doi":"10.1007/7651_2024_531","DOIUrl":"https://doi.org/10.1007/7651_2024_531","url":null,"abstract":"<p><p>Microbial dysbiosis is an important trigger in the development of oral diseases. Oral keratinocytes or gingival epithelial cells (GECs) offer protection against various microbial insults. Recent studies suggest that GECs expressed higher level of bitter taste receptor 14 (T2R14) compared to other taste receptors and toll-like receptors and act as innate immune sentinels. Macroautophagy or autophagy is a cellular conserved process involved in the regulation of host innate immune responses against microbial infection. Here, we describe a robust method for evaluation of T2R14-dependent autophagy flux in GECs. Autophagy flux was detected using Western blot analysis in GECs and further was confirmed using Acridine Orange-dependent flow cytometry analysis.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regeneration of Volumetric Muscle Loss Using MSCs Encapsulated in PRP-Derived Fibrin Microbeads. 利用包裹在 PRP 衍生的纤维蛋白微珠中的间充质干细胞再生体积损失的肌肉
Methods in molecular biology Pub Date : 2024-04-06 DOI: 10.1007/7651_2024_533
Sukran Seker, Özge Lalegül-Ülker, A. E. Elçin, YaşarMurat Elçin
{"title":"Regeneration of Volumetric Muscle Loss Using MSCs Encapsulated in PRP-Derived Fibrin Microbeads.","authors":"Sukran Seker, Özge Lalegül-Ülker, A. E. Elçin, YaşarMurat Elçin","doi":"10.1007/7651_2024_533","DOIUrl":"https://doi.org/10.1007/7651_2024_533","url":null,"abstract":"","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"11 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140734828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Standardizing Chondrocyte Isolation and Articular Cartilage Decellularization: A Versatile Bioink for Tissue Engineering Applications. 软骨细胞分离和关节软骨脱细胞标准化：组织工程应用的多功能生物墨水。
Methods in molecular biology Pub Date : 2024-03-21 DOI: 10.1007/7651_2024_534
Upasna Upadhyay, Kamma Srinivasulu, Lakshmi Kiran Chelluri
{"title":"Standardizing Chondrocyte Isolation and Articular Cartilage Decellularization: A Versatile Bioink for Tissue Engineering Applications.","authors":"Upasna Upadhyay, Kamma Srinivasulu, Lakshmi Kiran Chelluri","doi":"10.1007/7651_2024_534","DOIUrl":"https://doi.org/10.1007/7651_2024_534","url":null,"abstract":"<p><p>The extracellular matrix (ECM) is a noncellular component of tissues that provides structural and biochemical support to cells. The purpose of decellularization is to provide a tissue-specific niche to preserve the architecture, composition, and signaling molecules of the ECM. The current protocol discusses the standardization of chondrocyte isolation and the preparation of acellular ECM as a bioink additive from human native articular cartilage. Isolated chondrocytes with bioink additives provide a tissue-specific microenvironment. Herein, we discuss a standardized protocol with multiple applications in the area of organ-on-a-chip model development, spheroid formation, microfluidics platform, bioprinting, and tissue engineering. Cartilage tissue engineering is complex owing to the heterogeneous complex proteins, which are a challenge to synthesize; hence, this protocol in many ways offers cues to exploit the acellular ECM for multiple ongoing research studies.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140175414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation of Murine Neonatal and Adult Osteomacs to Examine Their Role in the Hematopoietic Niche. 分离小鼠新生儿和成年骨小梁以研究其在造血区的作用
Methods in molecular biology Pub Date : 2024-03-21 DOI: 10.1007/7651_2024_535
Safa F Mohamad, Melissa A Kacena
{"title":"Isolation of Murine Neonatal and Adult Osteomacs to Examine Their Role in the Hematopoietic Niche.","authors":"Safa F Mohamad, Melissa A Kacena","doi":"10.1007/7651_2024_535","DOIUrl":"10.1007/7651_2024_535","url":null,"abstract":"<p><p>Maintenance of hematopoietic stem cell (HSC) function is an orchestrated event between multiple cell types, and crosstalk between these cell types is an essential part of HSC regulation. Among the cell groups of the niche involved in this process are a group of bone-resident macrophages known as osteomacs (OM). Previously, it was demonstrated that OM and osteoblasts contained within neonatal calvarial cells are critical to maintain hematopoietic function. Additionally, interactions between neonatal calvarial cells and megakaryocytes further enhance this hematopoietic activity. In this chapter, we explore one such interaction involving OM and osteoblasts in the hematopoietic niche. We describe a protocol to isolate OM from both neonatal and adult mice, and subsequently use colony-forming assays to demonstrate their interaction with osteoblasts in maintaining HSC function.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140175411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Simplified Quantification of Progenitor Zone Size, an Indicator of Germ Stem Cell Niche Activity, in the Nematode Caenorhabditis elegans. 线虫 Caenorhabditis elegans 中作为生殖干细胞生态位活动指标的祖细胞区大小的简化定量。
Methods in molecular biology Pub Date : 2024-03-21 DOI: 10.1007/7651_2024_536
Sarah R Fausett, Caroline A Laury, Rachel E Magallon, Christian Braendle
{"title":"Simplified Quantification of Progenitor Zone Size, an Indicator of Germ Stem Cell Niche Activity, in the Nematode Caenorhabditis elegans.","authors":"Sarah R Fausett, Caroline A Laury, Rachel E Magallon, Christian Braendle","doi":"10.1007/7651_2024_536","DOIUrl":"https://doi.org/10.1007/7651_2024_536","url":null,"abstract":"<p><p>Germ stem cell (GSC) niches are fundamental for the maintenance of the immortal germ cell lineage across generations. In the nematode Caenorhabditis elegans, the simple GSC system has served as an important model for understanding stem cell biology and underlying genetic architecture. GSC niche activity in C. elegans is highly sensitive to subtle environmental and genetic variation. Quantifying variation in the C. elegans GSC niche is therefore essential; however, most methods to do so remain labor-intensive and time-consuming when screening large numbers of individuals. Here, we present a simple and efficient method to estimate the size of the C. elegans GSC niche progenitor pool. This method is ideal for detecting differences in progenitor pool size among different genotypes and environmental treatments during medium- to high-throughput applications such as forward genetic screens and quantitative genetics.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140175413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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