研究表皮细胞粘附的RNA干扰方法。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-17 DOI:10.1007/7651_2024_584

Anamika Dutta, Michele Calder, Lina Dagnino

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引用次数: 0

摘要

在本章中，我们提供了一种通过RNA干扰使表皮细胞靶基因沉默的方法。具体来说，我们描述了一种转染介导的小干扰RNA寡核苷酸（siRNA）递送方案。功能分析对于表征基因敲低的生物学后果是必不可少的，我们还提供了一种方法来分析细胞粘附特性的变化，这是由于基因敲低导致的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

RNA Interference Approaches to Study Epidermal Cell Adhesion.

In this chapter, we provide a method for silencing target genes in epidermal cells via RNA interference. Specifically, we describe a protocol for transfection-mediated delivery of small interfering RNA oligonucleotides (siRNA). Functional assays are indispensable to characterize the biological consequences of gene knockdowns, and we also provide a method to analyze alterations in cell adhesion properties, consequent to knockdown of genes involved in this process.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.