Methods in molecular biology最新文献_第4页

Isolation Procedure for Rat Pancreatic Ductal Cells. 大鼠胰腺导管细胞的分离程序

Methods in molecular biology Pub Date : 2024-07-06 DOI: 10.1007/7651_2024_556

Nazli Karimi, Gulbahar Boyuk Ozcan

引用次数: 0

Upstream Process Protocol for MSCs Isolated from Different Human-Based Tissue Origins. 从不同人体组织来源分离的间充质干细胞的上游工艺规程。

Methods in molecular biology Pub Date : 2024-07-06 DOI: 10.1007/7651_2024_553

Pelin Kılıç, Cansu Özdemir, Begüm Coşar, Büşra Nigar Savran, Aysun Sarıkaya, Begüm Sargon, Alım Toprakkale, İrem Songür, Özlem Kandemir Seçgin, Pınar Akpınar Oktar, Elif NazIı Çetindağ, Deniz Yurtsever Sarıca, Serpil Taşdelen, Üstün Ezer, Ahmet Emin Kürekçi, Günhan Gürman

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Establishing Neural Organoid Cultures for Investigating the Effects of Microgravity in Low-Earth Orbit (LEO). 建立用于研究低地轨道（LEO）微重力影响的神经器官培养物。

Methods in molecular biology Pub Date : 2024-05-28 DOI: 10.1007/7651_2024_550

Nicolette A Pirjanian, Kriti Kalpana, Ilya Kruglikov, Pinar Mesci, Jana Stoudemire, Paula Grisanti, Scott A Noggle, Jeanne F Loring, Valentina Fossati

{"title":"Establishing Neural Organoid Cultures for Investigating the Effects of Microgravity in Low-Earth Orbit (LEO).","authors":"Nicolette A Pirjanian, Kriti Kalpana, Ilya Kruglikov, Pinar Mesci, Jana Stoudemire, Paula Grisanti, Scott A Noggle, Jeanne F Loring, Valentina Fossati","doi":"10.1007/7651_2024_550","DOIUrl":"https://doi.org/10.1007/7651_2024_550","url":null,"abstract":"Recent findings from studies involving astronauts and animal models indicate that microgravity increases immune cell activity and potentially alters the white and gray matter of the central nervous system (CNS). To further investigate the impact of microgravity on CNS cells, we established cultures of three-dimensional neural organoids containing isogenic microglia, the brain's resident immune cells, and sent them onboard the International Space Station. When using induced pluripotent stem cell (iPSC) lines from individuals affected by neuroinflammatory and neurodegenerative diseases such as multiple sclerosis (MS) and Parkinson's disease (PD), these cultures can provide novel insights into pathogenic pathways that may be exacerbated by microgravity. We have devised a cryovial culture strategy that enables organoids to be maintained through space travel and onboard the International Space Station (ISS) without the need for medium or carbon dioxide exchange. Here, we provide a comprehensive description of all the steps involved: generating various types of neural organoids, establishing long-term cultures, arranging plans for shipment to the Kennedy Space Center (KSC), and ultimately preparing organoids for launch into low-Earth orbit (LEO) and return to Earth for post-flight analyses.","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Three-Dimensional Multicellular Model of Human Neuroblastoma Using Matrigel as an Extracellular Matrix Analogue. 利用 Matrigel 作为细胞外基质模拟物开发人神经母细胞瘤三维多细胞模型

Methods in molecular biology Pub Date : 2024-05-25 DOI: 10.1007/7651_2024_548

Kristina V Kitaeva, Valeriya V Solovyeva, Albert A Rizvanov

引用次数: 0

Telocytes: Detection, Visualization, Tissue Dissociation, and Tamoxifen-Induction of Transgenic Mice. 端细胞：转基因小鼠的检测、可视化、组织分离和他莫昔芬诱导。

Methods in molecular biology Pub Date : 2024-05-23 DOI: 10.1007/7651_2024_549

Marco Canella, Michal Shoshkes-Carmel

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Electrospinning Approach for Preparing Nanostructured Scaffolds for Stem Cell Seeding and/or Implantation in Neurotrauma. 电纺丝法制备纳米结构支架，用于干细胞播种和/或植入神经创伤。

Methods in molecular biology Pub Date : 2024-05-23 DOI: 10.1007/7651_2024_547

Eldar F Davletshin, Albert A Rizvanov, Yana O Mukhamedshina

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A Coculture System for Modeling Intestinal Epithelial-Fibroblast Crosstalk. 用于模拟肠道上皮细胞-成纤维细胞串联的共培养系统

Methods in molecular biology Pub Date : 2024-05-04 DOI: 10.1007/7651_2024_544

Rebecca F Lee, Mei-Lan Li, Maria Figetakis, Kaelyn Sumigray

引用次数: 0

Microfluidic Chip Fabrication for Tumor Cell 3D Culture Based on Microwell Arrays. 基于微孔阵列的肿瘤细胞三维培养微流控芯片制造技术

Methods in molecular biology Pub Date : 2024-05-04 DOI: 10.1007/7651_2024_543

Lanjie Lei

{"title":"Microfluidic Chip Fabrication for Tumor Cell 3D Culture Based on Microwell Arrays.","authors":"Lanjie Lei","doi":"10.1007/7651_2024_543","DOIUrl":"https://doi.org/10.1007/7651_2024_543","url":null,"abstract":"Compared with traditional 2D cell culture, 3D cell culture more closely resembles the original state of cells in vivo and enables the establishment of in vivo-like microenvironments and cell-cell interactions, thereby providing valuable cellular materials for numerous studies. The direct establishment of in vitro patient tumor models can enhance drug testing, cancer research, and individualized precision therapy. In this study, we propose a microfluidic chip based on microwell arrays for 3D tumor cell culture. This chip combines nanoscale channels and microwell arrays to precisely control cell distribution and nutrient diffusion, thus closely mimicking the tumor microenvironment. The incorporation of microwell arrays allows for simple and rapid high-throughput preparation of tumor spheroids, while promoting the formation of cell-cell and cell-matrix interactions, ultimately enhancing cell viability and function. Preliminary experiments using tumor cell lines validate the ability of the chip to support 3D tumor growth with enhanced physiological relevance. The microfluidic chip serves as a reliable and scalable platform for studying tumor biology and evaluating therapeutic efficacy and is anticipated to expedite cancer research and drug discovery.","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Stiffness-Dependent Autophagosome Formation and Apoptosis in Embryonal Rhabdomyosarcoma Tumor Cells. 评估胚胎横纹肌肉瘤肿瘤细胞中自噬体的形成和凋亡对硬度的依赖性

Methods in molecular biology Pub Date : 2024-04-23 DOI: 10.1007/7651_2024_538

Serap Sezen, Sevin Adiguzel, Atefeh Zarepour, Arezoo Khosravi, Joseph W Gordon, Saeid Ghavami, Ali Zarrabi

{"title":"Assessment of Stiffness-Dependent Autophagosome Formation and Apoptosis in Embryonal Rhabdomyosarcoma Tumor Cells.","authors":"Serap Sezen, Sevin Adiguzel, Atefeh Zarepour, Arezoo Khosravi, Joseph W Gordon, Saeid Ghavami, Ali Zarrabi","doi":"10.1007/7651_2024_538","DOIUrl":"https://doi.org/10.1007/7651_2024_538","url":null,"abstract":"Remodeling of the extracellular matrix (ECM) eventually causes the stiffening of tumors and changes to the microenvironment. The stiffening alters the biological processes in cancer cells due to altered signaling through cell surface receptors. Autophagy, a key catabolic process in normal and cancer cells, is thought to be involved in mechano-transduction and the level of autophagy is probably stiffness-dependent. Here, we provide a methodology to study the effect of matrix stiffness on autophagy in embryonal rhabdomyosarcoma cells. To mimic stiffness, we seeded cells on GelMA hydrogel matrices with defined stiffness and evaluated autophagy-related endpoints. We also evaluated autophagy-dependent pathways, apoptosis, and cell viability. Specifically, we utilized immunocytochemistry and confocal microscopy to track autophagosome formation through LC3 lipidation. This approach suggests that the use of GelMA hydrogels with defined stiffness represents a novel method to evaluate the role of autophagy in embryonal rhabdomyosarcoma and other cancer cells.","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods for Generating Self-Organizing Human Patterned Heart Organoids Using Pluripotent Stem Cells. 利用多能干细胞生成自组织人模式化心脏有器官的方法。

Methods in molecular biology Pub Date : 2024-04-23 DOI: 10.1007/7651_2024_545

B. Volmert, Aitor Aguirre

引用次数: 0