衰老相关β-半乳糖苷酶染色法检测小鼠肌肉组织切片细胞衰老。

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-04-03 DOI:10.1007/7651_2025_612

Wenxin Zhang, Tom H Cheung

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引用次数: 0

摘要

衰老相关β-半乳糖苷酶（SA-β-gal）的染色是细胞衰老的重要指标。这种染色法是基于衰老细胞中β-半乳糖苷酶活性的升高，该酶在酸性条件下裂解X-Gal底物，产生不溶性蓝色产物。这使得X-Gal可以使用显微镜进行可视化。在这项研究中，我们描述了SA-β-gal+细胞在小鼠肌肉组织中的鉴定。

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Detection of Cellular Senescence on Murine Muscle Tissue Sections by Senescence-Associated β-Galactosidase Staining.

Staining for the presence of senescence-associated beta-galactosidase (SA-β-gal) serves as a crucial indicator for cellular senescence. This staining assay is based on the elevated activity of β-galactosidase in senescent cells, which cleaves the X-Gal substrate to produce an insoluble blue product in acidic conditions. This enables X-Gal to be visualized using microscopy. In this study, we describe the identification of SA-β-gal⁺ cells within murine muscle tissues.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.